FastQCFastQC Report
Fri 27 May 2016
SRR939456_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939456_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1975143
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88500.44806882337126985No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77570.39273105795377855No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54170.2742586232996801No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT48030.2431722665143739No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT30030.1520396244727597No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21260.10763777610026211No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT20700.10480253834785633No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC19760.10004338926346093No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCATA2150.024.58187364-65
GTACATG98950.023.4571511
TACATGG100700.022.6689662
GAGTACT80850.021.6903112-13
CGCATAG2450.021.57489266-67
ACATGGG102200.021.0776423
GGTATCA138200.021.0196551
GTACTTT86400.020.18729414-15
AGTACTT85450.019.16372912-13
CATGGGG38850.018.932234
GTATCAA172500.018.601151
AACGCAT3100.018.59860264-65
CGCGTGA650.00707749218.48198766-67
CATGGGA68850.018.4021434
CGATTCG1559.765287E-418.3483319
AGTACAT76700.018.0058562
AGAGTAC150900.017.33867310-11
ATAGGAC5200.017.3367353
ACTTTTT105050.017.21222516-17
CGCGGAA2550.016.72893782-83
Produced by FastQC (version 0.11.5)