FastQCFastQC Report
Fri 27 May 2016
SRR939455_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939455_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1687624
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89000.5273686555773087No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75180.4454783767000232No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51200.3033851142197551No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36510.21633965859693866No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA22670.1343308699094111No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA20310.12034671230084426No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17940.10630329978715639No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17740.10511820168473546No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17500.10369608396183036No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA134450.027.9041351
GGTATCA96050.027.832851
GTACATG93650.025.975371
TACATGG94300.025.2777162
TATCAAC158500.023.4214652
ACATGGG98400.023.28173
ATCAACG162650.022.8954333
TCAACGC165650.022.4369494
GAGTACT87050.022.3548612-13
CAACGCA166900.022.0978935
AACGCAG170150.021.6464886
GTACTTT95400.020.70515814-15
TACCTGG9950.020.6184442
CATGGGA70800.020.09174
GTCTACG1756.543405E-520.0626261
ACGCAGA181850.019.9435217
AGTACTT89550.019.67009412-13
CGCAGAG182800.019.6541678
GTATAGG6000.019.2266851
CATGGGG27450.019.018564
Produced by FastQC (version 0.11.5)