##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939455_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1687624 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.066547998843344 31.0 26.0 31.0 10.0 33.0 2 27.17744710907169 31.0 26.0 31.0 16.0 34.0 3 27.675584727403734 31.0 26.0 31.0 16.0 34.0 4 31.395672258749578 35.0 32.0 37.0 19.0 37.0 5 31.70393701440605 35.0 32.0 37.0 19.0 37.0 6 31.74039596497798 35.0 32.0 37.0 17.0 37.0 7 31.19019520935943 35.0 30.0 37.0 17.0 37.0 8 31.13059544069058 35.0 30.0 37.0 17.0 37.0 9 32.13249159765445 35.0 30.0 39.0 17.0 39.0 10-11 31.820251134138886 35.0 30.0 39.0 16.5 39.0 12-13 31.654065123510925 35.0 30.0 39.0 16.5 39.0 14-15 32.35565949524302 36.0 30.0 39.0 16.5 41.0 16-17 32.338950797096985 36.0 30.0 39.0 17.0 40.0 18-19 32.53961842211298 36.5 30.5 39.0 17.5 41.0 20-21 32.36247203168479 36.0 30.0 39.0 16.0 41.0 22-23 32.19296982029172 36.0 30.0 39.0 15.5 41.0 24-25 32.069529705669034 36.0 30.0 39.0 13.5 41.0 26-27 32.060555550288456 36.0 30.0 39.0 15.5 41.0 28-29 32.08158481984139 36.0 30.0 39.0 15.0 41.0 30-31 31.7386061705688 36.0 29.5 39.0 12.5 40.5 32-33 31.148096969467133 35.5 28.0 39.0 9.5 40.0 34-35 30.71610263897645 35.0 27.0 39.0 9.0 40.0 36-37 30.458456682294162 35.0 26.5 39.0 9.0 40.0 38-39 30.047275933501773 35.0 25.0 38.0 8.5 40.0 40-41 29.940817089588677 35.0 25.5 38.0 8.0 40.0 42-43 29.45072747247017 35.0 25.0 38.0 6.5 40.0 44-45 29.244460258920235 34.5 24.5 38.0 2.0 40.0 46-47 28.59312263869203 34.0 24.0 38.0 2.0 40.0 48-49 28.0132289538428 33.0 22.5 38.0 2.0 40.0 50-51 27.544900404355474 33.0 22.5 37.5 2.0 39.5 52-53 27.9274518494641 33.5 23.0 38.0 2.0 39.5 54-55 27.815325570150698 33.5 22.5 38.0 2.0 40.0 56-57 27.35683214981536 33.0 21.0 38.0 2.0 40.0 58-59 26.873345306774496 33.0 19.5 38.0 2.0 40.0 60-61 26.331651481609647 32.5 18.0 37.0 2.0 39.0 62-63 25.771734699198397 31.5 14.0 37.0 2.0 39.0 64-65 25.234145757585814 31.0 13.0 36.0 2.0 39.0 66-67 24.812056773309692 31.0 8.0 36.0 2.0 39.0 68-69 24.363134797798562 31.0 8.0 35.5 2.0 38.5 70-71 23.84917197195584 30.0 6.5 35.0 2.0 38.0 72-73 23.185740129317907 29.5 2.0 35.0 2.0 37.5 74-75 22.52049153128896 29.0 2.0 34.0 2.0 37.0 76-77 21.8181789901068 28.5 2.0 34.0 2.0 36.0 78-79 21.043155347399654 27.5 2.0 34.0 2.0 36.0 80-81 20.29963427872559 26.0 2.0 33.0 2.0 35.0 82-83 19.44458866429963 26.0 2.0 32.5 2.0 35.0 84-85 18.61516753731874 24.5 2.0 32.0 2.0 34.5 86-87 17.96695561333567 23.5 2.0 32.0 2.0 34.0 88-89 17.067789092831106 20.0 2.0 31.0 2.0 34.0 90-91 16.185000331827467 15.5 2.0 31.0 2.0 34.0 92-93 15.291410290443842 4.5 2.0 30.5 2.0 34.0 94-95 14.365043990841562 2.0 2.0 30.0 2.0 34.0 96-97 13.31074457343579 2.0 2.0 29.0 2.0 34.0 98-99 12.24985571430603 2.0 2.0 29.0 2.0 33.0 100-101 10.91312875379824 2.0 2.0 26.0 2.0 32.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 3.685104303455521 1101 2 3.534959064800475 1101 3 3.2118431655603423 1101 4 3.0681209632541133 1101 5 2.329755039399135 1101 6 2.306934949154975 1101 7 2.8964956987690726 1101 8 2.9111587574621787 1101 9 2.994762969094783 1101 10-11 3.047507183613444 1101 12-13 3.09150793787445 1101 14-15 2.890065713497627 1101 16-17 2.9138207750742353 1101 18-19 2.9014450557485816 1101 20-21 2.6046653052627704 1101 22-23 2.9477786305977 1101 24-25 3.1099660521539256 1101 26-27 3.05583890706518 1101 28-29 2.4886550173828645 1101 30-31 2.8257465374034574 1101 32-33 3.066204769164802 1101 34-35 3.060346970925611 1101 36-37 3.126686801964457 1101 38-39 3.1421620533371453 1101 40-41 2.993876154840663 1101 42-43 2.9182119673782907 1101 44-45 2.5979747766238184 1101 46-47 2.674285718440416 1101 48-49 2.7124616641691794 1101 50-51 2.74453729074704 1101 52-53 2.4861424042757108 1101 54-55 2.50253705160247 1101 56-57 2.653495980677029 1101 58-59 2.5952116404662 1101 60-61 2.387820104129645 1101 62-63 2.3064967482323233 1101 64-65 2.488941592393889 1101 66-67 2.5637955547092943 1101 68-69 2.3335632488061755 1101 70-71 2.06174596540993 1101 72-73 2.1328632360223203 1101 74-75 1.989367112090882 1101 76-77 1.8254889137084866 1101 78-79 1.6662341493873107 1101 80-81 1.709132776450101 1101 82-83 1.7411874459945516 1101 84-85 1.7556835598706897 1101 86-87 1.4984751935229284 1101 88-89 1.1954930331945697 1101 90-91 1.390893265251897 1101 92-93 1.0597291192234142 1101 94-95 0.6860610921085755 1101 96-97 0.6499211986004312 1101 98-99 0.1753767036039342 1101 100-101 0.028449521780844478 1102 1 3.793896126425473 1102 2 3.751260313332171 1102 3 3.241202132632452 1102 4 2.893878323717903 1102 5 2.6362006553288033 1102 6 2.3365615263972153 1102 7 3.1136518789062393 1102 8 2.9925205379934177 1102 9 3.327879609237254 1102 10-11 3.3966792498481517 1102 12-13 3.537614683545325 1102 14-15 3.4847849122775116 1102 16-17 3.1237283903597515 1102 18-19 3.035360263584586 1102 20-21 3.343695064460988 1102 22-23 3.5272357608693774 1102 24-25 3.504184251540078 1102 26-27 3.4034195216686456 1102 28-29 3.0609944275984695 1102 30-31 3.384884832655626 1102 32-33 3.4690930644169704 1102 34-35 3.9146356015193753 1102 36-37 3.700141649260882 1102 38-39 3.720559399970469 1102 40-41 3.403691527505586 1102 42-43 3.6611581895752607 1102 44-45 3.3616134223742655 1102 46-47 3.4321849549225476 1102 48-49 3.299921048997337 1102 50-51 3.3111491328523037 1102 52-53 3.2388860005774767 1102 54-55 3.098145521345945 1102 56-57 3.253150574099031 1102 58-59 3.2293790649669774 1102 60-61 3.3521130922695406 1102 62-63 3.1817270587548485 1102 64-65 3.1294120652823736 1102 66-67 2.9306488380255793 1102 68-69 2.773716260249472 1102 70-71 2.385421650302508 1102 72-73 2.6843314211990865 1102 74-75 2.5646496421200595 1102 76-77 2.3902339972596955 1102 78-79 2.608374107138058 1102 80-81 2.332210364453587 1102 82-83 2.3089117648911426 1102 84-85 2.1752820972508573 1102 86-87 1.7577604375455138 1102 88-89 1.536649819599024 1102 90-91 1.4713444607352066 1102 92-93 1.2161022672663098 1102 94-95 1.3668850472447946 1102 96-97 1.157569402533591 1102 98-99 0.627223450601015 1102 100-101 0.42938817037316923 1103 1 3.789395706394071 1103 2 3.8771076886624023 1103 3 3.043761633181184 1103 4 2.9994910664644685 1103 5 2.6926597789022786 1103 6 2.560477459276431 1103 7 3.0319586960510065 1103 8 3.0881244823105938 1103 9 3.178567805535831 1103 10-11 3.3252247711901894 1103 12-13 3.3307737876967316 1103 14-15 3.422901943088398 1103 16-17 3.1050542504881165 1103 18-19 3.179386574632467 1103 20-21 3.3105356912086705 1103 22-23 3.287149496481252 1103 24-25 3.2983855366723738 1103 26-27 3.2341167386973524 1103 28-29 3.054364600454843 1103 30-31 3.17521896237162 1103 32-33 3.44469263291953 1103 34-35 3.607362447727045 1103 36-37 3.48910833690962 1103 38-39 3.3820866294714165 1103 40-41 3.223475240583184 1103 42-43 3.2681500744281493 1103 44-45 3.0228063232585534 1103 46-47 3.0223325860156365 1103 48-49 3.0300488251241084 1103 50-51 2.952400487131829 1103 52-53 2.6715391131388735 1103 54-55 2.729562115277364 1103 56-57 2.7878081373552916 1103 58-59 2.8738356449519564 1103 60-61 3.045596638504584 1103 62-63 2.9111899801610086 1103 64-65 2.5720426786686197 1103 66-67 2.620328409055343 1103 68-69 2.6786838628412646 1103 70-71 2.4240513768257976 1103 72-73 2.3528953467025957 1103 74-75 2.418370196046709 1103 76-77 2.3377736545421506 1103 78-79 2.0958416102795177 1103 80-81 1.8391759827187535 1103 82-83 1.7860984436946516 1103 84-85 1.8304421654854863 1103 86-87 1.716965326289838 1103 88-89 1.9900506114576686 1103 90-91 1.8859192579150275 1103 92-93 1.710813849602994 1103 94-95 1.4076307258353626 1103 96-97 0.8675271887925948 1103 98-99 0.28265583724518706 1103 100-101 0.04119608381160056 1104 1 3.6524667241652757 1104 2 3.4030104534512517 1104 3 2.8877139296040006 1104 4 2.859641192981524 1104 5 2.4420489561943803 1104 6 2.14107547147054 1104 7 2.65291570848391 1104 8 2.738525678429088 1104 9 3.028359531755502 1104 10-11 3.141969724330064 1104 12-13 3.0279980587385005 1104 14-15 2.79175563149294 1104 16-17 2.5488347401579503 1104 18-19 2.8093130053457003 1104 20-21 2.9805823219494414 1104 22-23 2.955394244347051 1104 24-25 2.743412963405998 1104 26-27 2.836976400636246 1104 28-29 2.84832824871037 1104 30-31 2.804950706213102 1104 32-33 2.9043598065955045 1104 34-35 3.2387160920182403 1104 36-37 3.1068847775630637 1104 38-39 2.999042267920842 1104 40-41 2.8806375412056013 1104 42-43 2.776437794568416 1104 44-45 2.7255856401017304 1104 46-47 2.815656296759176 1104 48-49 2.9902177145149125 1104 50-51 2.5699710655341725 1104 52-53 2.41557587190243 1104 54-55 2.540504399736651 1104 56-57 2.7731269238737823 1104 58-59 2.848965426396827 1104 60-61 2.796530959035639 1104 62-63 2.446012756289928 1104 64-65 2.4529698940111224 1104 66-67 2.639071284751015 1104 68-69 2.581235437216943 1104 70-71 2.5076714468660164 1104 72-73 2.8263591143582225 1104 74-75 2.674193745041336 1104 76-77 2.289761872163254 1104 78-79 2.3113826221571827 1104 80-81 2.3435734944911317 1104 82-83 2.177158787214047 1104 84-85 1.6372125043818073 1104 86-87 1.5997729462516546 1104 88-89 1.77323854722394 1104 90-91 1.6688698639146793 1104 92-93 1.5695564665004031 1104 94-95 1.498278933128061 1104 96-97 1.271197771547456 1104 98-99 0.7377266950320163 1104 100-101 0.5637506461308082 1105 1 3.710875668866368 1105 2 3.353308095890263 1105 3 2.106276047362705 1105 4 2.747006598925058 1105 5 2.651490552780441 1105 6 2.4820901629266707 1105 7 3.189565462655999 1105 8 3.026362945882287 1105 9 3.455510052957248 1105 10-11 3.628785110333734 1105 12-13 3.720927267868337 1105 14-15 3.4437274944580736 1105 16-17 3.1758411703990035 1105 18-19 2.903962282125512 1105 20-21 3.114719071548457 1105 22-23 3.2510597262658187 1105 24-25 3.096700566052615 1105 26-27 3.201491745595824 1105 28-29 3.298849887097358 1105 30-31 3.2131745158616702 1105 32-33 3.6217795304648313 1105 34-35 3.768607106855246 1105 36-37 3.9314086224338602 1105 38-39 3.9228086891962306 1105 40-41 3.484284061336126 1105 42-43 3.4759847085475712 1105 44-45 3.2640435175108884 1105 46-47 3.210168614353119 1105 48-49 3.4523690944405665 1105 50-51 3.192583449206193 1105 52-53 2.829609021556177 1105 54-55 3.1952647866572654 1105 56-57 3.1811476962595506 1105 58-59 3.3113568123772126 1105 60-61 3.430824607680748 1105 62-63 3.605663503088632 1105 64-65 3.589309343205233 1105 66-67 3.628644338868085 1105 68-69 3.66621737490561 1105 70-71 3.588590892741667 1105 72-73 3.3722941482559854 1105 74-75 3.2484221306695 1105 76-77 3.2348670003459894 1105 78-79 3.0718463912856215 1105 80-81 3.1825534469265584 1105 82-83 3.047649395103246 1105 84-85 3.107272149306745 1105 86-87 2.7865362266351994 1105 88-89 2.894405654896275 1105 90-91 3.0659855704115806 1105 92-93 2.8867824680781258 1105 94-95 2.7567586133916766 1105 96-97 2.6045696746833986 1105 98-99 2.30174834651819 1105 100-101 1.9358523073977043 1106 1 3.3023121921555685 1106 2 2.5683921794160227 1106 3 2.480621666205817 1106 4 1.3164509763493122 1106 5 1.5507699990454675 1106 6 2.0914665866128352 1106 7 2.5555795104024384 1106 8 2.5278178200869768 1106 9 2.945428119596759 1106 10-11 2.683602119205471 1106 12-13 2.9749842678271783 1106 14-15 2.693028300450713 1106 16-17 1.4384876193620286 1106 18-19 2.463868725099296 1106 20-21 1.7509521239174006 1106 22-23 2.72931552355136 1106 24-25 2.8282502320318876 1106 26-27 2.799150832989042 1106 28-29 2.262717601549735 1106 30-31 1.6236000498903316 1106 32-33 1.7234250100794206 1106 34-35 2.441402052050865 1106 36-37 2.810296696041899 1106 38-39 1.2388113119720856 1106 40-41 1.130807535038116 1106 42-43 2.656988459627975 1106 44-45 2.765681049319852 1106 46-47 2.8140215969800693 1106 48-49 2.9193239636778756 1106 50-51 2.6631210756791965 1106 52-53 2.2881119672116377 1106 54-55 2.3235278930854797 1106 56-57 2.638964859829649 1106 58-59 2.753053555338081 1106 60-61 3.115805242000789 1106 62-63 2.5254471524655138 1106 64-65 2.54911771593461 1106 66-67 2.5096153052502856 1106 68-69 2.6099967267904454 1106 70-71 2.3203971593025514 1106 72-73 2.3642543751658387 1106 74-75 2.4139683932848683 1106 76-77 2.5168129921682656 1106 78-79 2.4055767364332716 1106 80-81 2.3157761259899097 1106 82-83 2.5009177172788988 1106 84-85 2.331163253015678 1106 86-87 2.142830817727699 1106 88-89 1.9116118025239288 1106 90-91 1.892617815510551 1106 92-93 1.9584888477533173 1106 94-95 1.7068787529892049 1106 96-97 1.9335499286441742 1106 98-99 1.6138704531976114 1106 100-101 1.511159468796345 1107 1 2.527800718405942 1107 2 2.961384503396964 1107 3 -0.2658360869186822 1107 4 1.6394882401419828 1107 5 0.5076392880642757 1107 6 1.3559624091092068 1107 7 2.229624035916288 1107 8 1.5892432349602679 1107 9 -1.7412829881897345 1107 10-11 -1.0206087584155377 1107 12-13 1.394323059918694 1107 14-15 1.2932240849697862 1107 16-17 -0.5341221120348436 1107 18-19 0.7123956659697868 1107 20-21 0.9263448437829851 1107 22-23 0.714519191238427 1107 24-25 0.011625091055531556 1107 26-27 0.5539271984458054 1107 28-29 0.7963328396559355 1107 30-31 1.2541313581509748 1107 32-33 0.11035045947754085 1107 34-35 1.0709114255404089 1107 36-37 1.9443617638256363 1107 38-39 0.2468412104691069 1107 40-41 0.8464314561373847 1107 42-43 0.6087201672257407 1107 44-45 0.8203765691486069 1107 46-47 0.8541007451375933 1107 48-49 0.6548839563125775 1107 50-51 1.4837034806783898 1107 52-53 1.4858182242604556 1107 54-55 1.136020556560517 1107 56-57 1.1069308586327864 1107 58-59 1.24260389828466 1107 60-61 1.7582768165629048 1107 62-63 1.9440515727937289 1107 64-65 2.177070883011737 1107 66-67 1.4626037164179166 1107 68-69 0.9303142976238732 1107 70-71 0.811846079826406 1107 72-73 1.3959281673724355 1107 74-75 1.112169362281577 1107 76-77 1.0107863965460133 1107 78-79 1.8446404232240745 1107 80-81 2.239121811537821 1107 82-83 2.0881363399686172 1107 84-85 2.180907625469473 1107 86-87 2.1267193773520674 1107 88-89 2.3176040171762615 1107 90-91 2.2121651950764587 1107 92-93 2.179675724867961 1107 94-95 1.898370397981953 1107 96-97 1.9261368736576383 1107 98-99 1.7260584351066033 1107 100-101 1.7347114401637587 1108 1 2.8124766465269424 1108 2 3.2037918098309888 1108 3 2.714252576492253 1108 4 2.7095181107028843 1108 5 2.3737527961957063 1108 6 2.340948896236423 1108 7 2.1259654848755574 1108 8 2.432244989263051 1108 9 2.762509506239166 1108 10-11 -0.23975199486710252 1108 12-13 2.1840242357756985 1108 14-15 1.694049970418554 1108 16-17 1.7824430105292848 1108 18-19 0.37048442420789485 1108 20-21 -0.23380581289521984 1108 22-23 1.0912500835621621 1108 24-25 0.043871665423740325 1108 26-27 0.9666153711260961 1108 28-29 0.4103859310219242 1108 30-31 0.37063751006411394 1108 32-33 1.8125338552387653 1108 34-35 -0.38044549210219003 1108 36-37 0.8911435664902783 1108 38-39 1.6723248290741992 1108 40-41 2.1768443099612895 1108 42-43 2.023219835330618 1108 44-45 2.1125328798972696 1108 46-47 2.5819873436867375 1108 48-49 2.081789982579707 1108 50-51 2.2832047489918885 1108 52-53 1.9605506338190324 1108 54-55 2.1964471010581725 1108 56-57 2.5431906969524505 1108 58-59 2.4856498064919528 1108 60-61 2.0958491222857205 1108 62-63 2.224401899947491 1108 64-65 2.409013121407259 1108 66-67 2.473966975628006 1108 68-69 2.4943872434301433 1108 70-71 1.9104660480593019 1108 72-73 2.115632958515576 1108 74-75 2.082045731348952 1108 76-77 1.9692823189773812 1108 78-79 2.0200944083362096 1108 80-81 1.8987728475247359 1108 82-83 1.819086152684939 1108 84-85 2.09800644724179 1108 86-87 1.3818480459784936 1108 88-89 1.5775078268581169 1108 90-91 1.838800963887799 1108 92-93 1.8699805349765395 1108 94-95 1.8462468618765069 1108 96-97 1.9625019108773056 1108 98-99 1.8032237544371235 1108 100-101 1.5628081401779834 1109 1 2.468376530553659 1109 2 2.8261034383082055 1109 3 2.4019808787243697 1109 4 2.400088843551952 1109 5 2.002924270640225 1109 6 0.7667711655701375 1109 7 -0.9171317584565664 1109 8 -2.8178070725917124 1109 9 -2.525589359076374 1109 10-11 -2.0261435246526425 1109 12-13 0.17292919458669687 1109 14-15 1.8974755818007125 1109 16-17 1.2467611351527452 1109 18-19 -0.1094405245954988 1109 20-21 -1.6424547095065378 1109 22-23 0.43376209298550705 1109 24-25 0.7872065747891916 1109 26-27 -0.8962729149859108 1109 28-29 -0.844943296415348 1109 30-31 -0.5544819975568593 1109 32-33 -0.31535176471966864 1109 34-35 -0.5353748258336353 1109 36-37 1.3014033008838943 1109 38-39 -0.4876847151103334 1109 40-41 -0.24605252309408598 1109 42-43 0.8387582311684838 1109 44-45 0.7371347900247223 1109 46-47 0.09644103444694707 1109 48-49 0.7991848894931088 1109 50-51 0.6858801710448823 1109 52-53 0.8528591473841551 1109 54-55 1.6203200572270724 1109 56-57 0.49531974817134383 1109 58-59 1.5166600469854608 1109 60-61 2.034989693986482 1109 62-63 2.1874989907603783 1109 64-65 2.2788496853131157 1109 66-67 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-2.2164784480919444 2312 66-67 1.116541843508216 2312 68-69 -0.5232392140818121 2312 70-71 -1.184913953618409 2312 72-73 -1.1994773510222139 2312 74-75 -0.9879895005946757 2312 76-77 -1.79164671382188 2312 78-79 0.13574410215383992 2312 80-81 0.6206602730762825 2312 82-83 -0.6306512854494457 2312 84-85 -1.4169142808850452 2312 86-87 -0.46709751228004137 2312 88-89 -2.5037630396799244 2312 90-91 -2.598988651077388 2312 92-93 -1.904776616269162 2312 94-95 -1.587791809375906 2312 96-97 -1.312776169820621 2312 98-99 -0.542858788973664 2312 100-101 -0.07056447956868261 2313 1 -2.0518339995510644 2313 2 -0.7973980244840746 2313 3 -2.3024046985236595 2313 4 -1.5910920087670206 2313 5 -1.4761493009126134 2313 6 -2.8017473048070656 2313 7 0.7927786342676661 2313 8 -2.351366107208797 2313 9 -3.1741280665799074 2313 10-11 -4.649209049348137 2313 12-13 -3.5138654805694287 2313 14-15 -3.0919353218509684 2313 16-17 -1.5267720231387756 2313 18-19 -3.6678128357273216 2313 20-21 -6.256997244466572 2313 22-23 -4.865950947762705 2313 24-25 -1.3628597157975513 2313 26-27 -4.81760888527813 2313 28-29 -5.718125904485003 2313 30-31 -5.280282533880705 2313 32-33 -4.705367982416757 2313 34-35 0.4684516308095219 2313 36-37 0.8096559290106988 2313 38-39 -2.354237664591505 2313 40-41 -6.163518842876677 2313 42-43 -2.3967651245150066 2313 44-45 -0.8088348822818112 2313 46-47 -2.8664332374064507 2313 48-49 -2.6672228806035356 2313 50-51 -2.019055328114238 2313 52-53 -4.229693130192434 2313 54-55 -3.487705603129154 2313 56-57 -2.7549317161207263 2313 58-59 -0.5324435802994678 2313 60-61 1.3353234465936126 2313 62-63 1.0233498999312722 2313 64-65 1.7910265561974477 2313 66-67 1.0319850805685533 2313 68-69 -1.983212047939407 2313 70-71 -0.043957419446261525 2313 72-73 -1.7070953092721446 2313 74-75 -2.2016640788045727 2313 76-77 -1.2847708201987729 2313 78-79 -0.4344932441429883 2313 80-81 -0.6987964040755621 2313 82-83 -0.7305540592555637 2313 84-85 -1.1241948070503298 2313 86-87 -1.1084157850853025 2313 88-89 -1.6011722475724035 2313 90-91 -3.261161942470011 2313 92-93 -3.2633954323972727 2313 94-95 -2.33568285884688 2313 96-97 -1.8844862069953425 2313 98-99 -1.1776600471949976 2313 100-101 -0.9558675965260743 2314 1 -1.8229897954280503 2314 2 -0.28672155720909487 2314 3 -2.135664808921316 2314 4 -0.8185815885800203 2314 5 -2.0442381822914584 2314 6 -2.277634428835455 2314 7 -0.5157152262708031 2314 8 0.6079191242883333 2314 9 -1.426279694382096 2314 10-11 -2.0513564527501877 2314 12-13 -1.0141090876438916 2314 14-15 -3.4433124762950342 2314 16-17 -1.4516945758029607 2314 18-19 -1.108931738509792 2314 20-21 -1.2531347346096382 2314 22-23 -2.110390229051429 2314 24-25 -2.2803050802224654 2314 26-27 -1.819121220526526 2314 28-29 -1.4905166333348383 2314 30-31 -1.5885975614801175 2314 32-33 -8.15817720850665 2314 34-35 -8.766218021099906 2314 36-37 -4.814366826424699 2314 38-39 -3.0820454825480255 2314 40-41 -2.038400559672894 2314 42-43 -1.1930420198625349 2314 44-45 -1.0604781739343956 2314 46-47 -2.4240695447174794 2314 48-49 -2.059542921157778 2314 50-51 -1.243396321693151 2314 52-53 -0.6342822368726146 2314 54-55 0.4402613338991195 2314 56-57 -0.07952335744099415 2314 58-59 -2.637443861926144 2314 60-61 -3.8092791781670066 2314 62-63 -2.936819639327485 2314 64-65 -3.2244854410989348 2314 66-67 -3.1891749397085682 2314 68-69 -4.326619167461768 2314 70-71 -6.017460573665026 2314 72-73 -6.022624204169066 2314 74-75 -6.08344987355505 2314 76-77 -6.9876490448242095 2314 78-79 -6.308900419990682 2314 80-81 -5.2790600066439985 2314 82-83 -4.346810959109121 2314 84-85 -4.477829199299961 2314 86-87 -4.226928514611043 2314 88-89 -4.082387748304633 2314 90-91 -4.790865107953845 2314 92-93 -5.125009716502262 2314 94-95 -4.435868732452828 2314 96-97 -4.317851927396379 2314 98-99 -4.5983075069223815 2314 100-101 -4.267149561153763 2315 1 -24.654807977246232 2315 2 -12.91721719151829 2315 3 -8.28186408986636 2315 4 -5.747947279386388 2315 5 -2.340886872584214 2315 6 -2.4532122408539436 2315 7 -8.018745529301107 2315 8 -1.8021348048333934 2315 9 -5.470861101675368 2315 10-11 -3.836673607347006 2315 12-13 -4.127019976190372 2315 14-15 -3.765153770593958 2315 16-17 -6.159251072978101 2315 18-19 -7.255972056948416 2315 20-21 -5.714581832709278 2315 22-23 -7.29125181097233 2315 24-25 -23.976151253822877 2315 26-27 -10.342927075688312 2315 28-29 -7.249793726298375 2315 30-31 -5.599723652902821 2315 32-33 -6.1676340652092705 2315 34-35 -6.780567019559076 2315 36-37 -6.021942584000453 2315 38-39 -4.283188266317925 2315 40-41 -4.082815043494158 2315 42-43 -3.8928365755892926 2315 44-45 -8.667662303364288 2315 46-47 -4.79349044867228 2315 48-49 -4.49933490883112 2315 50-51 -6.432071206130747 2315 52-53 -9.828998466970202 2315 54-55 -5.885631064662771 2315 56-57 -12.59005237644459 2315 58-59 -7.898852439224552 2315 60-61 -5.166013898249183 2315 62-63 -5.657325545850341 2315 64-65 -5.696634901807716 2315 66-67 -8.896359069138459 2315 68-69 -6.215807662582463 2315 70-71 -6.333414605508889 2315 72-73 -6.274627285833166 2315 74-75 -5.562102929538614 2315 76-77 -6.4371225938740295 2315 78-79 -5.939607918706654 2315 80-81 -5.24223412066557 2315 82-83 -4.5128677130896016 2315 84-85 -4.8195022861001675 2315 86-87 -4.427808072905854 2315 88-89 -5.872231097046287 2315 90-91 -5.317919550686254 2315 92-93 -5.378296824874116 2315 94-95 -5.625275512113847 2315 96-97 -5.938454135922317 2315 98-99 -5.455440275283964 2315 100-101 -4.947722237065422 2316 1 -1.9808420405065732 2316 2 0.07966309220475054 2316 3 -4.6552733026174025 2316 4 -0.1726348953705532 2316 5 -5.425387264352949 2316 6 -0.7933499784926106 2316 7 -3.203417062147821 2316 8 -3.829168909332548 2316 9 -2.9005221186245187 2316 10-11 -1.7037093613987189 2316 12-13 -3.7689835623176364 2316 14-15 -3.803747114649383 2316 16-17 -1.3656544298175604 2316 18-19 -6.569922116744696 2316 20-21 -2.381278397516077 2316 22-23 -4.950505800827585 2316 24-25 -1.747145934015876 2316 26-27 -2.171382888299142 2316 28-29 -4.769666710751341 2316 30-31 -8.61886464840779 2316 32-33 -6.33514303465131 2316 34-35 -2.917487098737599 2316 36-37 -4.499071537771744 2316 38-39 -5.448612613713692 2316 40-41 -2.817647407715363 2316 42-43 -3.1550968749830837 2316 44-45 -0.3239182314437947 2316 46-47 -2.272822586080011 2316 48-49 -2.3512648853249978 2316 50-51 0.14113696740217918 2316 52-53 1.0395703399909095 2316 54-55 1.4278055718995653 2316 56-57 -0.684765560493414 2316 58-59 -2.994900729875713 2316 60-61 -5.796762588916842 2316 62-63 -1.640817648617464 2316 64-65 -4.007652881930607 2316 66-67 -3.91100915780191 2316 68-69 -0.5725326821709018 2316 70-71 1.0520456196550363 2316 72-73 1.589861971455793 2316 74-75 1.571175762366206 2316 76-77 0.5420899509731818 2316 78-79 0.421018292683506 2316 80-81 1.1070050232166544 2316 82-83 0.5905839756398308 2316 84-85 0.3738324905916528 2316 86-87 0.12170840200032629 2316 88-89 -1.4316799402975562 2316 90-91 -1.2996961187921556 2316 92-93 -1.9621806963739719 2316 94-95 -2.9593480511876216 2316 96-97 -4.76121899220505 2316 98-99 -2.8595909619102162 2316 100-101 -1.9574061415563282 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 25270.0 3 7931.0 4 8139.0 5 9133.0 6 10006.0 7 12358.0 8 15676.0 9 18639.0 10 21380.0 11 24140.0 12 27337.0 13 29872.0 14 32146.0 15 34794.0 16 37069.0 17 38230.0 18 36493.0 19 36747.0 20 39002.0 21 41774.0 22 45405.0 23 48781.0 24 52865.0 25 56811.0 26 61068.0 27 66178.0 28 70615.0 29 75569.0 30 81263.0 31 86667.0 32 92044.0 33 96975.0 34 100620.0 35 99675.0 36 87157.0 37 51386.0 38 8348.0 39 61.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.172589343602034 19.962265958073832 14.69527888563626 21.16986581268787 2 15.50225265370597 21.27101298733228 40.90382736006195 22.322906998899793 3 19.501797818897973 26.60196954459447 30.311951377728846 23.584281258778706 4 12.21364183644845 17.55815685258876 37.92485320602117 32.30334810494162 5 12.57588203234323 38.57610099616574 34.41513028048308 14.432886691007946 6 29.668959977503924 36.84729777109646 18.552977616044355 14.93076463535527 7 27.112809876972193 34.36640738770108 21.199343176040646 17.321439559286084 8 25.89630545731164 34.851346356948156 20.350125766830807 18.902222418909396 9 27.11044865743359 17.081652887854332 21.233512745673366 34.574385709038715 10-11 24.41660431568551 27.66177834974411 27.298087570706013 20.623529763864365 12-13 26.022696280346768 25.41387150245385 28.012332428306035 20.551099788893346 14-15 23.108455621435873 26.19008002251938 27.538559069835124 23.16290528620962 16-17 20.840742646016118 29.74333962865326 28.289067325509578 21.12685039982105 18-19 20.4569426918276 27.781537682803336 31.748595178492963 20.012924446876102 20-21 21.757674547379086 26.66242911824867 31.831199573396386 19.74869676097586 22-23 21.246492951327696 26.971659559425564 31.380622939473845 20.40122454977289 24-25 20.824732730510174 27.158100506304173 31.885928973238208 20.13123778994745 26-27 20.916603004195636 27.246547900652697 31.720715683948715 20.116133411202952 28-29 20.42464034026814 27.29063808293917 32.110697607296615 20.17402396949607 30-31 20.54633108864543 27.48325093508871 31.999880061643566 19.970537914622298 32-33 20.402537849645444 27.557974459376677 31.570644109850754 20.46884358112713 34-35 20.849542948519055 27.403338981837276 31.536134513950216 20.210983555693453 36-37 20.988587756392242 27.26793609333714 31.511802911081706 20.231673239188908 38-39 20.94359615401182 27.40366091923122 31.667494971501466 19.9852479552555 40-41 20.841206769858438 27.325215649490943 31.83168260957121 20.001894971079416 42-43 20.556675703050878 27.635424051031226 31.380655013174596 20.427245232743296 44-45 20.637522298382706 28.000714616610267 30.932542031950604 20.429221053056427 46-47 20.758096861005185 27.92169063674971 30.307279196422076 21.01293330582303 48-49 21.155771075437986 27.99096930888505 30.052355115772055 20.800904499904913 50-51 21.126055820240627 28.406716626221524 29.801628733752157 20.665598819785693 52-53 20.869972775481184 28.543063509818467 29.916378016275306 20.670585698425043 54-55 20.568637389160603 28.43507810378543 30.039519820220768 20.956764686833203 56-57 20.84706258162217 29.066027157563923 29.35049312481204 20.736417136001865 58-59 20.838734554387763 28.493304959545384 29.572906020683103 21.09505446538375 60-61 20.948416229426357 28.422272335762568 29.83155241630303 20.797759018508042 62-63 21.165941214160284 28.33372061724449 29.765068534413814 20.73526963418141 64-65 20.95474083888878 28.25152122594267 29.869443871547848 20.924294063620703 66-67 20.639627890853205 28.63150403017332 29.671442486853483 21.057425592119987 68-69 20.73893039503276 28.576571934512142 29.68662671747727 20.99787095297783 70-71 20.478996555527722 28.732075624036028 29.50705220376173 21.281875616674522 72-73 20.679814745566226 28.557999233106283 29.236263558380898 21.52592246294659 74-75 20.65957643917682 28.678513273243173 29.27432754879406 21.387582738785948 76-77 20.84130070215778 28.479671629595142 29.351994593670128 21.32703307457695 78-79 20.59574339217512 28.51474172002964 29.333950491192734 21.555564396602506 80-81 20.3470276953482 28.991483607681793 29.118834582693214 21.54265411427679 82-83 20.61817737988224 28.86847145097543 28.7920025729179 21.721348596224427 84-85 20.51544295229288 28.426432408601798 28.892860482756472 22.16526415634885 86-87 20.598816010895334 28.405527103127216 29.210655130149455 21.785001755828 88-89 20.89484008245229 28.134113210414824 29.046948172845838 21.924098534287044 90-91 20.440271552054305 28.49230180921883 29.23281734959447 21.834609289132395 92-93 20.398066959391482 28.54862539961212 29.05070654421345 22.002601096782946 94-95 20.245030118112165 28.43206791442056 28.840144657193267 22.482757310274003 96-97 20.45987859076917 28.195277500449894 28.633561141443952 22.711282767336986 98-99 20.330351958633983 28.154433526432165 28.56387631180734 22.951338203126507 100-101 20.484801480999437 28.153609118962823 28.809094002160286 22.552495397877454 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4924.0 1 3337.5 2 1770.5 3 1812.5 4 1624.0 5 1598.0 6 2486.5 7 4290.5 8 6103.0 9 6788.0 10 5986.0 11 4780.0 12 4297.0 13 4145.0 14 3987.5 15 3933.5 16 3936.0 17 3995.5 18 4186.5 19 4503.0 20 4968.0 21 5569.5 22 6486.0 23 7551.5 24 8757.0 25 10442.5 26 12900.0 27 15713.5 28 18128.5 29 20715.0 30 23803.0 31 27219.5 32 31064.0 33 35067.0 34 39561.5 35 43646.5 36 47486.5 37 51212.0 38 53305.0 39 54422.5 40 54867.5 41 55678.0 42 57006.0 43 58104.0 44 59386.0 45 60412.0 46 60573.0 47 61361.5 48 66174.0 49 66182.0 50 60085.5 51 55421.0 52 51160.5 53 47837.5 54 44150.0 55 39595.0 56 34935.5 57 30068.0 58 25843.0 59 22496.0 60 18893.0 61 15363.0 62 12216.5 63 9336.5 64 7040.0 65 5213.5 66 3786.5 67 2746.0 68 1995.0 69 1464.5 70 1064.5 71 780.0 72 599.5 73 477.5 74 376.5 75 289.5 76 193.0 77 127.0 78 83.0 79 56.5 80 46.0 81 31.0 82 18.0 83 15.5 84 12.5 85 5.0 86 1.5 87 3.0 88 1.5 89 1.0 90 2.0 91 1.5 92 0.5 93 0.0 94 0.5 95 0.5 96 0.5 97 1.5 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.487506695804279 2 3.5940470152119195 3 1.0725730375960523 4 0.931664873218205 5 0.954892796025655 6 0.1183320455267287 7 1.7746844083753253 8 0.9173844410840329 9 0.13670106611425292 10-11 1.0057927595246334 12-13 0.8192286907510204 14-15 0.6426787009428641 16-17 0.1330272619967481 18-19 0.1449078704735178 20-21 0.15924755751281092 22-23 0.5990374633212137 24-25 1.3635738766455086 26-27 0.2140583447497784 28-29 0.2237168942845089 30-31 0.2029776774921428 32-33 0.22265030599233007 34-35 0.6911492133318796 36-37 0.27298734789265855 38-39 0.26472128862827266 40-41 0.25011495451593485 42-43 0.19684479481211453 44-45 0.16618038141197328 46-47 0.2018518342948429 48-49 0.6063554441036628 50-51 0.22884244357747932 52-53 0.24975942508520857 54-55 0.27677966182040553 56-57 0.20238512844093234 58-59 0.1899415983655127 60-61 0.17806098988874297 62-63 0.16671367555806269 64-65 0.16096594976132125 66-67 0.17521675444293278 68-69 0.18176442145880836 70-71 0.1887861277156523 72-73 0.24943352310704278 74-75 0.21983569799908037 76-77 0.21758401160448063 78-79 0.21094746223092345 80-81 0.21894687442226465 82-83 0.23301991438851305 84-85 0.20392575597407955 86-87 0.191600735708902 88-89 0.19293397107412552 90-91 0.20899205036192897 92-93 0.19758548112612762 94-95 0.20765881499670544 96-97 0.23091636525671594 98-99 0.24392281693078555 100-101 0.294882035334885 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1687624.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.3460364280519 #Duplication Level Percentage of deduplicated Percentage of total 1 84.28395676934709 57.604743796581474 2 9.691688757405988 13.247770257260214 3 2.8439654923179942 5.831213074142645 4 1.1629431686683722 3.1793022467825067 5 0.5769118114733135 1.9714817841364247 6 0.32950253448998557 1.351211533523279 7 0.21868042401394444 1.0462158158031218 8 0.14961525036324905 0.8180487481214981 9 0.10784109874772584 0.6633460497107891 >10 0.5710188758105261 7.156600988225223 >50 0.04222051968550022 1.9665056456834737 >100 0.019720318138932044 2.3582612440537125 >500 9.214188273918298E-4 0.44920299138764525 >1k 7.371350619134639E-4 0.9997332802858361 >5k 2.76425648217549E-4 1.3563625443021314 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8900 0.5273686555773087 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7518 0.4454783767000232 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5120 0.3033851142197551 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3651 0.21633965859693866 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2267 0.1343308699094111 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2031 0.12034671230084426 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1794 0.10630329978715639 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1774 0.10511820168473546 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1750 0.10369608396183036 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 1.1850981024209184E-4 0.0 0.0 0.0 0.0 6 1.1850981024209184E-4 0.0 0.0 5.925490512104592E-5 0.0 7 1.7776471536313777E-4 5.925490512104592E-5 0.0 5.925490512104592E-5 0.0 8 1.7776471536313777E-4 5.925490512104592E-5 0.0 5.925490512104592E-5 0.0 9 1.7776471536313777E-4 5.925490512104592E-5 0.0 5.925490512104592E-5 0.0 10-11 1.7776471536313777E-4 5.925490512104592E-5 0.0 1.7776471536313777E-4 0.0 12-13 1.7776471536313777E-4 5.925490512104592E-5 0.0 2.370196204841837E-4 0.0 14-15 2.370196204841837E-4 5.925490512104592E-5 0.0 2.962745256052296E-4 0.0 16-17 2.370196204841837E-4 5.925490512104592E-5 0.0 4.1478433584732143E-4 0.0 18-19 2.370196204841837E-4 5.925490512104592E-5 0.0 4.444117884078444E-4 0.0 20-21 2.370196204841837E-4 5.925490512104592E-5 0.0 7.999412191341199E-4 0.0 22-23 2.370196204841837E-4 5.925490512104592E-5 0.0 0.001451745175465625 0.0 24-25 4.1478433584732143E-4 5.925490512104592E-5 0.0 0.0019257844164339926 0.0 26-27 4.1478433584732143E-4 5.925490512104592E-5 0.0 0.002251686394599745 0.0 28-29 5.925490512104592E-4 5.925490512104592E-5 0.0 0.0033775295918996176 0.0 30-31 7.110588614525511E-4 5.925490512104592E-5 0.0 0.006992078804283419 0.0 32-33 7.110588614525511E-4 5.925490512104592E-5 0.0 0.013687883082961608 0.0 34-35 7.110588614525511E-4 5.925490512104592E-5 0.0 0.025598119012291837 0.0 36-37 7.703137665735969E-4 5.925490512104592E-5 0.0 0.045981806373931636 0.0 38-39 8.295686716946429E-4 5.925490512104592E-5 0.0 0.07347608235009695 0.0 40-41 8.591961242551658E-4 5.925490512104592E-5 0.0 0.10479229970656971 0.0 42-43 9.184510293762118E-4 5.925490512104592E-5 0.0 0.13830094855252117 0.0 44-45 0.0011258431972998725 5.925490512104592E-5 0.0 0.18049044099870587 0.0 46-47 0.001392490270344579 5.925490512104592E-5 0.0 0.23337544381923936 0.0 48-49 0.0015998824382682398 5.925490512104592E-5 0.0 0.29106009395457755 0.0 50-51 0.0015998824382682398 5.925490512104592E-5 0.0 0.3531888619739942 0.0 52-53 0.0015998824382682398 5.925490512104592E-5 0.0 0.41386588481794523 0.0 54-55 0.0016591373433892857 5.925490512104592E-5 0.0 0.48277933947372165 0.0 56-57 0.0017183922485103317 5.925490512104592E-5 0.0 0.5651732850445359 0.0 58-59 0.0017183922485103317 5.925490512104592E-5 0.0 0.6638327020710775 0.0 60-61 0.0017183922485103317 5.925490512104592E-5 0.0 0.7770688257573962 0.0 62-63 0.0017183922485103317 8.888235768156889E-5 0.0 0.8856238119391523 0.0 64-65 0.0017183922485103317 1.1850981024209184E-4 0.0 0.9932603470915322 0.0 66-67 0.0018369020587524236 1.1850981024209184E-4 0.0 1.1160661379549 0.0 68-69 0.0018961569638734695 1.1850981024209184E-4 0.0 1.264677439998483 0.0 70-71 0.0018961569638734695 1.1850981024209184E-4 0.0 1.4287838997312197 0.0 72-73 0.0018961569638734695 1.1850981024209184E-4 0.0 1.5900164965655856 0.0 74-75 0.0018961569638734695 1.1850981024209184E-4 0.0 1.7578263878683877 0.0 76-77 0.0018961569638734695 1.1850981024209184E-4 0.0 1.9365095542609017 0.0 78-79 0.0018961569638734695 1.1850981024209184E-4 0.0 2.1435758202063964 0.0 80-81 0.0020739216792366073 1.1850981024209184E-4 0.0 2.374699577631036 0.0 82-83 0.002133176584357653 1.1850981024209184E-4 0.0 2.6044012173327706 0.0 84-85 0.002222058942039222 1.1850981024209184E-4 0.0 2.8439095438320385 0.0 86-87 0.002251686394599745 1.1850981024209184E-4 0.0 3.103357145904538 0.0 88-89 0.002251686394599745 1.1850981024209184E-4 0.0 3.3777073566149802 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 13445 0.0 27.904135 1 GGTATCA 9605 0.0 27.83285 1 GTACATG 9365 0.0 25.97537 1 TACATGG 9430 0.0 25.277716 2 TATCAAC 15850 0.0 23.421465 2 ACATGGG 9840 0.0 23.2817 3 ATCAACG 16265 0.0 22.895433 3 TCAACGC 16565 0.0 22.436949 4 GAGTACT 8705 0.0 22.35486 12-13 CAACGCA 16690 0.0 22.097893 5 AACGCAG 17015 0.0 21.646488 6 GTACTTT 9540 0.0 20.705158 14-15 TACCTGG 995 0.0 20.618444 2 CATGGGA 7080 0.0 20.0917 4 GTCTACG 175 6.543405E-5 20.062626 1 ACGCAGA 18185 0.0 19.943521 7 AGTACTT 8955 0.0 19.670094 12-13 CGCAGAG 18280 0.0 19.654167 8 GTATAGG 600 0.0 19.226685 1 CATGGGG 2745 0.0 19.01856 4 >>END_MODULE