FastQCFastQC Report
Fri 27 May 2016
SRR939455_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939455_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1687624
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128890.7637364721051609No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120470.7138438419932401No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87810.5203173218679042No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA56400.334197664882699No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA31450.18635667660568941No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA30970.18351244115987922No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30670.18173479400624784No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30580.18120149986015843No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28210.16715808734647056No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT17840.10571075073594592No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA107400.030.2343351
GTATCAA147950.025.0236231
GTACATG99650.022.2629471
TACATGG99750.021.9021282
GAGTACT101400.021.30636412-13
ACATGGG100950.020.7436683
ATCGCAT704.365398E-420.68011364-65
TATCAAC178950.020.550782
ATCAACG180700.020.2670693
TCAACGC181150.020.1905694
GTACTTT108300.020.1457614-15
AGTACTT103750.019.95610412-13
CAACGCA183600.019.8953365
AACGCAG186800.019.6306046
TACCGTC1300.00767829818.2220087
CATGGGA72600.018.0764334
ACTTTTT125250.017.9490316-17
ACCGTCC1600.001212187317.7664598
ACGCAGA205100.017.763577
CGCAGAG205250.017.6813418
Produced by FastQC (version 0.11.5)