FastQCFastQC Report
Fri 27 May 2016
SRR939454_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939454_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1682417
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92400.5492098570092908No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70140.41690020963887076No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50680.3012332852081262No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36920.2194461896188638No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA22330.13272571544391193No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA18350.10906927355108752No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18130.10776163103439873No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16930.10062903548882353No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA126250.029.4589751
GGTATCA88450.027.5765651
GTACATG92550.026.6816271
TACATGG93200.025.7396952
TATCAAC149800.024.707752
ACATGGG97250.024.1048853
ATCAACG158150.023.8623163
CAACGCG2252.2046152E-923.7177526
TCAACGC161800.023.5805024
GAGTACT81600.023.57440412-13
CAACGCA162050.022.983075
AACGCAG163650.022.6782116
CATGGGA68150.022.183754
AGTACTT84250.022.11936412-13
ACGCGTT653.0435572E-421.81455486-87
GTACTTT91600.021.39359514-15
AACGCCG2507.4451236E-921.3459786
AACGCGG3001.2551027E-1021.023847
ACGCAGA177150.020.4582927
CATGGGG28800.020.2026774
Produced by FastQC (version 0.11.5)