FastQCFastQC Report
Fri 27 May 2016
SRR939454_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939454_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1682417
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128790.7655058169288589No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115470.6863340063729741No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86880.5163999174996449No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA55120.3276238887267544No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30800.18306995233643028No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA30380.18057354389547894No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA29580.1758184801984288No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28780.17106341650137866No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27000.16048339977544213No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT16970.10086678867367602No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA105250.029.1226861
GTATCAA144150.025.0490381
GTACATG99050.023.233161
TACATGG97400.022.8418622
GAGTACT100850.021.47876512-13
ACATGGG101250.021.3161223
GCACCGT2701.027729E-921.068726
AGTACTT103250.020.68110512-13
TCGGACG1150.003767603820.6107049
TATCAAC177300.020.3339862
ATCAACG177850.020.3232383
TCAACGC179700.020.1428054
CAACGCA181700.019.9323775
GTACTTT108650.019.89317714-15
TAGCGCG1200.004799436319.7666114
CGCGCGT1200.004820117719.7519257
AACGCAG184900.019.664336
GAGTCCG1250.00610271418.961859
CATGGGA71300.018.4303554
TACCTGG9750.018.001752
Produced by FastQC (version 0.11.5)