FastQCFastQC Report
Fri 27 May 2016
SRR939453_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939453_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1321390
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126970.9608821014235011No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100870.763362822482386No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64630.489106168504378No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50340.3809624713370012No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26420.19994097124997162No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20030.1515828029574917No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17930.1356904471806204No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA15710.11888995678792788No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA104700.035.8549271
GTATCAA162400.034.7045671
TATCAAC200550.027.793662
ATCAACG208850.027.1139033
TCAACGC211900.026.7225554
CAACGCA212800.026.2901925
AACGCAG215400.026.0925316
GTACATG132000.025.7092861
TACATGG131900.025.4222642
GAGTACT112300.023.9716912-13
CGCAGAG234950.023.5267478
ACGCAGA235400.023.4967677
AACGCGG2703.092282E-1123.3686146
ACATGGG136100.023.0916393
AGTACTT113350.022.8833212-13
GCAGAGT228050.022.2028649
GTACTTT122950.022.19307514-15
CAACGCG2456.184564E-921.6911935
CATGGGA104450.021.2176254
ACTTTTT143600.020.55672316-17
Produced by FastQC (version 0.11.5)