FastQCFastQC Report
Fri 27 May 2016
SRR939453_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939453_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1321390
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183791.390883842014848No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167961.2710857506110989No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106670.8072559955804115No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA79450.6012607935582985No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45710.34592361074323247No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32510.24602880300289848No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27820.21053587510121916No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA21460.1624047404626946No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA18590.1406851875676371No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15420.11669529813302659No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14950.11313843755439347No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14010.10602471639712727No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA118400.037.3455621
GCGTATC753.1199033E-431.594961
GTATCAA177500.031.1588571
CACGCGA350.00831738627.19359890-91
CGTATCA950.001244341124.938672
TATCAAC224300.024.6951732
ATCAACG224450.024.610683
TCAACGC226050.024.394574
CAACGCA228900.024.0287465
AACGCAG232550.023.6108576
GAGTACT129800.023.21365512-13
GTACATG147400.022.7959541
TACATGG145650.022.6425652
GAACCGT1050.00223042122.5592886
AGTACTT131400.021.9935612-13
GTACTTT138100.021.69841214-15
ACGCAGA259700.021.0695237
CGCAGAG259300.021.0106758
ACATGGG149200.020.9247993
ATAACGC2057.0066926E-720.7985633
Produced by FastQC (version 0.11.5)