##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939453_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1321390 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.982868797251378 31.0 31.0 31.0 30.0 34.0 2 31.196241836248195 31.0 31.0 34.0 30.0 34.0 3 31.144108855069284 31.0 31.0 34.0 28.0 34.0 4 35.11888011866292 35.0 35.0 37.0 32.0 37.0 5 34.764742430319586 35.0 35.0 37.0 32.0 37.0 6 34.7616759624335 35.0 35.0 37.0 32.0 37.0 7 34.625797077320094 35.0 35.0 37.0 30.0 37.0 8 34.65369799983351 35.0 35.0 37.0 30.0 37.0 9 35.94428594131937 37.0 35.0 39.0 30.0 39.0 10-11 35.808376406662674 37.0 35.0 39.0 30.0 39.0 12-13 35.76213078652026 37.0 35.0 39.0 30.0 39.0 14-15 36.47054011306276 38.0 35.0 40.0 30.0 41.0 16-17 36.52103920871204 38.0 35.0 40.0 30.5 41.0 18-19 36.62434746743959 38.0 35.0 40.0 30.0 41.0 20-21 36.59900256548029 38.0 35.0 40.0 30.0 41.0 22-23 36.454097200675044 38.0 35.0 40.0 30.0 41.0 24-25 36.223658420299834 38.0 34.5 40.0 29.5 41.0 26-27 35.82320246104481 38.0 34.0 40.0 28.0 41.0 28-29 35.52438114409826 38.0 34.0 40.0 27.0 41.0 30-31 35.19583771634415 37.5 34.0 40.0 27.0 41.0 32-33 34.822430925010785 37.0 33.0 40.0 27.0 41.0 34-35 34.62272833909746 37.0 33.0 40.0 25.5 41.0 36-37 34.45866890168686 37.0 33.0 40.0 25.0 41.0 38-39 34.3556614625508 37.0 33.0 40.0 24.5 41.0 40-41 34.0588615019033 37.0 33.0 40.0 24.0 41.0 42-43 33.6259843800846 37.0 33.0 40.0 22.5 41.0 44-45 33.24779285449413 37.0 32.0 40.0 20.5 41.0 46-47 32.663600451040196 36.5 31.0 40.0 16.5 41.0 48-49 32.15247391004927 36.0 31.0 40.0 11.0 41.0 50-51 31.82626817215205 36.0 30.5 39.0 8.5 41.0 52-53 31.43958104723057 36.0 30.0 39.0 7.0 40.5 54-55 31.217368452917004 35.0 30.0 39.0 4.0 40.0 56-57 30.83464684915127 35.0 29.0 39.0 2.0 40.0 58-59 30.42257774010701 35.0 28.0 38.5 2.0 40.0 60-61 30.14048237083677 35.0 28.0 38.0 2.0 40.0 62-63 29.77840531561462 34.0 28.0 38.0 2.0 40.0 64-65 29.356772413897488 34.0 27.0 38.0 2.0 40.0 66-67 27.762452417530024 33.0 22.5 36.5 2.0 39.5 68-69 24.306718304209962 29.0 11.5 35.0 2.0 38.0 70-71 23.74322304542943 28.5 10.5 34.5 2.0 38.0 72-73 25.46398073241057 31.0 18.5 35.0 2.0 38.0 74-75 26.02226102815974 31.0 20.5 35.0 2.0 37.0 76-77 26.02204950847214 31.0 23.0 34.5 2.0 36.0 78-79 26.801009164591832 32.0 25.0 35.0 2.0 37.0 80-81 26.665079575295714 32.5 24.5 35.0 2.0 36.5 82-83 26.264589182603167 32.0 23.5 35.0 2.0 36.0 84-85 25.931440377178575 32.0 22.5 35.0 2.0 36.0 86-87 25.49253286312141 32.0 20.5 34.5 2.0 35.0 88-89 25.010403060413655 31.5 19.5 34.0 2.0 35.0 90-91 24.55296241079469 31.0 17.5 34.0 2.0 35.0 92-93 24.024980891334124 31.0 9.5 34.0 2.0 35.0 94-95 22.740922437736018 30.0 2.0 34.0 2.0 35.0 96-97 21.98266484535224 29.0 2.0 34.0 2.0 35.0 98-99 21.667029415993767 29.5 2.0 34.0 2.0 35.0 100-101 20.525119381863036 28.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4487399412256252 1101 2 0.3400689085415003 1101 3 0.34667527763327755 1101 4 0.388632613679853 1101 5 0.4428441663154459 1101 6 0.6342559233502385 1101 7 0.6037100150064063 1101 8 0.6115868312404515 1101 9 0.7055876304090347 1101 10-11 0.9145285255388771 1101 12-13 0.6697988573319762 1101 14-15 0.8566748228677099 1101 16-17 0.7432064875929925 1101 18-19 0.7460817264766959 1101 20-21 0.7152754177356329 1101 22-23 0.8488362185546521 1101 24-25 0.7561175242144742 1101 26-27 0.757450360519961 1101 28-29 0.6681485821560358 1101 30-31 0.823649421770206 1101 32-33 0.7464685384938647 1101 34-35 0.8714571980612789 1101 36-37 0.6536543078683721 1101 38-39 0.8127377257109174 1101 40-41 0.6789135636150334 1101 42-43 0.8896494010001064 1101 44-45 0.6895035912741037 1101 46-47 0.7600834299296295 1101 48-49 0.618309533691459 1101 50-51 0.7032588355054905 1101 52-53 0.8380109407792808 1101 54-55 0.650521504004427 1101 56-57 0.4660131198547681 1101 58-59 0.4247320862860313 1101 60-61 0.28427423703034194 1101 62-63 0.2442748992475039 1101 64-65 -0.031883096012577994 1101 66-67 -2.8453537458515257 1101 68-69 -7.787705597880354 1101 70-71 -10.839678599487886 1101 72-73 -10.81790182942288 1101 74-75 -9.40672436150987 1101 76-77 -4.5751473977444626 1101 78-79 -1.633167652758548 1101 80-81 -0.9364500409631802 1101 82-83 -0.8211038021169621 1101 84-85 -1.1089958616231463 1101 86-87 -1.1079099681519686 1101 88-89 -0.9503452024360115 1101 90-91 -1.1324263758075261 1101 92-93 -1.6116878744035397 1101 94-95 -4.987315151656038 1101 96-97 -2.129030501670467 1101 98-99 -1.3148257531135208 1101 100-101 -0.9289762134392134 1102 1 0.354841323993508 1102 2 0.16132465428501064 1102 3 0.22746223133765042 1102 4 0.11985686914896121 1102 5 0.3929267761808859 1102 6 0.5054802167137851 1102 7 0.3655406572033755 1102 8 0.490989690754418 1102 9 0.6563949713187256 1102 10-11 0.6111305316701987 1102 12-13 0.5141853653978004 1102 14-15 0.4250742756720882 1102 16-17 0.603421500609727 1102 18-19 0.6493154479753471 1102 20-21 0.7468916911212347 1102 22-23 0.6831072812282528 1102 24-25 0.5988324140742947 1102 26-27 0.720205816371049 1102 28-29 0.6856539157975874 1102 30-31 0.4387589745779934 1102 32-33 0.25381247974233645 1102 34-35 0.46924331050922063 1102 36-37 0.673955777035566 1102 38-39 0.6459951122834013 1102 40-41 0.49705249218781944 1102 42-43 0.6406161827395351 1102 44-45 0.3535935943435504 1102 46-47 0.16541271361115406 1102 48-49 0.013215479707049838 1102 50-51 0.1572204143391751 1102 52-53 0.1165994489133162 1102 54-55 -0.038973874769386185 1102 56-57 -0.15753078653082397 1102 58-59 0.06740036599242316 1102 60-61 -0.16620152758459383 1102 62-63 -0.29578425396347896 1102 64-65 -0.35849143051432364 1102 66-67 -3.0052227826887012 1102 68-69 -7.665476154663267 1102 70-71 -10.814323433908157 1102 72-73 -9.16005777638583 1102 74-75 -7.646668599850699 1102 76-77 -4.2694302645341615 1102 78-79 -1.8913623240432855 1102 80-81 -1.2562193775453316 1102 82-83 -1.1256694182236124 1102 84-85 -1.3187211838205677 1102 86-87 -1.2341093269781531 1102 88-89 -0.8313524175361877 1102 90-91 -0.8652502500586365 1102 92-93 -1.4201390910043301 1102 94-95 -4.800151227914796 1102 96-97 -2.0588779576276615 1102 98-99 -1.3153396963703976 1102 100-101 -1.0163285672519606 1103 1 0.26014001394499076 1103 2 0.09134618298963915 1103 3 0.18723150582215453 1103 4 0.14106440178910873 1103 5 0.4004710784787022 1103 6 0.25953096373947204 1103 7 0.2944737182963948 1103 8 0.2914604344850886 1103 9 0.4900084431416687 1103 10-11 0.6362129534736098 1103 12-13 0.5809687770033136 1103 14-15 0.7152675156958566 1103 16-17 0.5308537536821518 1103 18-19 0.6957863509036528 1103 20-21 0.7229653033713106 1103 22-23 0.6240935476547733 1103 24-25 0.8361683340608224 1103 26-27 0.9256073368936555 1103 28-29 0.847037287788794 1103 30-31 0.8357854032380061 1103 32-33 0.8320215617828097 1103 34-35 0.9284317912638969 1103 36-37 0.9600422731879803 1103 38-39 0.8158409497278001 1103 40-41 0.7387936516279652 1103 42-43 0.7679405146184948 1103 44-45 0.5528544359807697 1103 46-47 0.5421127500785232 1103 48-49 0.5337728010400937 1103 50-51 0.3469034814483898 1103 52-53 0.43472863595018296 1103 54-55 0.5111275674501989 1103 56-57 0.41470051653891815 1103 58-59 0.6389763671327238 1103 60-61 0.23622697693865646 1103 62-63 0.41288637166475084 1103 64-65 0.27660257939665556 1103 66-67 -2.5558397661154864 1103 68-69 -7.279331625416255 1103 70-71 -10.970660610815614 1103 72-73 -8.635218316102868 1103 74-75 -6.6606391186684135 1103 76-77 -3.275245606374078 1103 78-79 -0.7324136578788938 1103 80-81 -0.20141373750601232 1103 82-83 -0.1203575852108294 1103 84-85 -0.06415397001998357 1103 86-87 -0.15249415581291714 1103 88-89 -0.017406796489517262 1103 90-91 -0.054325681974802364 1103 92-93 0.08476780614103419 1103 94-95 0.8427684568738698 1103 96-97 0.835378709699242 1103 98-99 0.6173067423227749 1103 100-101 0.7616300561697145 1104 1 0.4046864424739418 1104 2 0.16486255701627073 1104 3 0.2262436203968825 1104 4 0.08815726067686569 1104 5 0.3523824108806366 1104 6 0.41167862229895036 1104 7 0.6184299444339061 1104 8 0.44770148533592646 1104 9 0.5607379434715014 1104 10-11 0.6565886507638652 1104 12-13 0.5476539252355295 1104 14-15 0.6516376355790001 1104 16-17 0.6924665813525266 1104 18-19 0.6912199403256167 1104 20-21 0.7654145774209056 1104 22-23 0.6213983955889901 1104 24-25 0.5674472858449775 1104 26-27 0.8484351353640776 1104 28-29 0.8569866830664807 1104 30-31 0.7878202510532972 1104 32-33 0.47125591260866884 1104 34-35 0.5711309781665008 1104 36-37 0.6440880159776583 1104 38-39 0.530462933092565 1104 40-41 0.961449397702232 1104 42-43 1.045647080423386 1104 44-45 0.6716982219203231 1104 46-47 0.6025520440827208 1104 48-49 0.36028373764374777 1104 50-51 0.35224140589538067 1104 52-53 0.42338671875308975 1104 54-55 0.4680979996901016 1104 56-57 0.21818269051998485 1104 58-59 0.3519853306920169 1104 60-61 0.05854570892027411 1104 62-63 0.12039889932991699 1104 64-65 0.2062639824768553 1104 66-67 -2.660788296785281 1104 68-69 -7.121621884807773 1104 70-71 -8.630230630788514 1104 72-73 -7.882627237209455 1104 74-75 -6.834000549470964 1104 76-77 -3.596894610336637 1104 78-79 -0.8473822620906759 1104 80-81 -0.26796521461311684 1104 82-83 -0.14948343460803315 1104 84-85 -0.041369264704883335 1104 86-87 0.185733590140579 1104 88-89 0.05128355585878097 1104 90-91 0.1529542249952165 1104 92-93 0.1820748499048328 1104 94-95 0.8538379539648524 1104 96-97 1.0197263790548874 1104 98-99 0.5643794727728029 1104 100-101 0.6640926790332387 1105 1 0.5725447228621867 1105 2 0.37371626093727883 1105 3 0.3978569508245222 1105 4 0.3982897491239399 1105 5 0.3744391389580173 1105 6 0.5614000668756418 1105 7 0.6839966384811476 1105 8 0.63299392662136 1105 9 0.9405372271062973 1105 10-11 0.9598857746861071 1105 12-13 0.9446666488202666 1105 14-15 0.9672120129503554 1105 16-17 0.9195178891939904 1105 18-19 0.9665141417907535 1105 20-21 0.9283066364958898 1105 22-23 0.9733630914131766 1105 24-25 1.0499418795787392 1105 26-27 0.9811030928292013 1105 28-29 0.8814077795187387 1105 30-31 1.005518159512775 1105 32-33 1.0815961302004027 1105 34-35 1.266686245015336 1105 36-37 1.0369740960295104 1105 38-39 0.8412722525456218 1105 40-41 0.9684740513938621 1105 42-43 1.029353543915029 1105 44-45 1.0345907000857082 1105 46-47 0.9896798219536223 1105 48-49 0.968930131510497 1105 50-51 0.5798480085250297 1105 52-53 0.9441240105350026 1105 54-55 0.9654381725788603 1105 56-57 1.1632518124976414 1105 58-59 1.3245502757352945 1105 60-61 1.3443919083290758 1105 62-63 1.228873466130132 1105 64-65 1.2007075421748858 1105 66-67 1.7965643649975824 1105 68-69 -4.519393860511705 1105 70-71 -4.805643015748199 1105 72-73 -1.3732605666312772 1105 74-75 1.1110140907538906 1105 76-77 1.1876957148216398 1105 78-79 0.9766102445728606 1105 80-81 1.05318027122442 1105 82-83 1.2697976577092014 1105 84-85 1.3320835932122925 1105 86-87 1.2536974813111819 1105 88-89 1.4757620676966923 1105 90-91 1.427257277649737 1105 92-93 1.4826017862422027 1105 94-95 2.0723370235841507 1105 96-97 1.9952235599734216 1105 98-99 2.048963232181382 1105 100-101 1.8671412823446119 1106 1 0.11315579735502368 1106 2 0.15403337519192917 1106 3 0.19766907090992802 1106 4 0.08840337844560509 1106 5 0.21461868117325622 1106 6 0.22729493299360115 1106 7 0.24577307546672955 1106 8 0.2226903892960692 1106 9 0.4370444071048354 1106 10-11 0.4576224836609626 1106 12-13 0.2353650438131396 1106 14-15 0.23278252566252888 1106 16-17 0.2668598250049783 1106 18-19 0.17280811892968728 1106 20-21 0.32173337459635576 1106 22-23 0.24259895253414498 1106 24-25 0.25118042228410076 1106 26-27 0.38369496244560963 1106 28-29 0.6061601836492798 1106 30-31 0.25081271127693583 1106 32-33 0.37005588108346643 1106 34-35 0.5435830716193593 1106 36-37 0.570307992720906 1106 38-39 0.48476729781144456 1106 40-41 0.373499627850002 1106 42-43 0.6039949665068107 1106 44-45 0.43937825775949335 1106 46-47 0.30231256319763844 1106 48-49 0.25292315713765134 1106 50-51 0.10399776036580377 1106 52-53 0.4507624811305284 1106 54-55 0.5517192769860166 1106 56-57 0.4158242462608186 1106 58-59 0.5851639416752299 1106 60-61 0.6184332137423851 1106 62-63 0.5454553474080548 1106 64-65 0.45815583998849263 1106 66-67 1.2614115259546175 1106 68-69 -5.04298518367079 1106 70-71 -5.945749462254536 1106 72-73 -2.2728490335649774 1106 74-75 0.35191515381055893 1106 76-77 0.659251766132293 1106 78-79 0.564399036000804 1106 80-81 0.8798582332202862 1106 82-83 0.8239835489698315 1106 84-85 0.7458771337487242 1106 86-87 0.8671873718507115 1106 88-89 0.8452221453274333 1106 90-91 0.6820500463856156 1106 92-93 0.4753006657230152 1106 94-95 1.4350727185144905 1106 96-97 1.612371052748351 1106 98-99 1.3818423580750085 1106 100-101 1.1391594281078774 1107 1 0.07156147702473703 1107 2 -0.09407918927669456 1107 3 -0.03628290398428646 1107 4 -0.01984817673267969 1107 5 0.22212555139571322 1107 6 0.23071182366595622 1107 7 0.18534084094027037 1107 8 0.07847359465677783 1107 9 0.24708757911119505 1107 10-11 0.09646742884923043 1107 12-13 0.2342289383825502 1107 14-15 0.15838691231564894 1107 16-17 0.030719239460523795 1107 18-19 0.1915409969634041 1107 20-21 0.08300975689369494 1107 22-23 0.16242206306220197 1107 24-25 0.5186325022144729 1107 26-27 0.46856418170538205 1107 28-29 0.5454214150160794 1107 30-31 0.252418264045815 1107 32-33 0.28094019982749785 1107 34-35 0.44209455914577944 1107 36-37 0.564813958346214 1107 38-39 0.45489201217738184 1107 40-41 0.5151955545904912 1107 42-43 0.8429115077731595 1107 44-45 0.7847285855151895 1107 46-47 0.7536315172946857 1107 48-49 0.8878924822910292 1107 50-51 0.9167461494540525 1107 52-53 1.0559101105286537 1107 54-55 0.6880069131578956 1107 56-57 0.8343676304831007 1107 58-59 0.6390517802024966 1107 60-61 0.8366169026981538 1107 62-63 0.8717834117845022 1107 64-65 0.8387597336147294 1107 66-67 1.6040394878672188 1107 68-69 -5.0374568517736655 1107 70-71 -5.075698487561816 1107 72-73 -1.4817338067511727 1107 74-75 0.9489857166757751 1107 76-77 0.8821878055401484 1107 78-79 0.9257362964004585 1107 80-81 0.8333122080887776 1107 82-83 0.9136222674429071 1107 84-85 0.9125523747959967 1107 86-87 0.9930507047981969 1107 88-89 0.7726893161676784 1107 90-91 0.6059481312977084 1107 92-93 0.8432552043570283 1107 94-95 1.3728355768700133 1107 96-97 1.778733328903428 1107 98-99 1.5730253224956137 1107 100-101 1.3235557702771104 1108 1 -0.04442939310355243 1108 2 -0.11718577838238176 1108 3 -0.008731584231281175 1108 4 -0.06081573325178624 1108 5 -0.060552258102880785 1108 6 -0.00827997900670141 1108 7 0.17112466713880536 1108 8 0.1112237896324828 1108 9 0.14672100092892038 1108 10-11 0.22545286350792537 1108 12-13 0.12093099880474512 1108 14-15 0.13227209249227911 1108 16-17 -0.11305739827580652 1108 18-19 0.14923234147295972 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86-87 0.15428636028143572 2316 88-89 0.49664639401338917 2316 90-91 0.6845498741884519 2316 92-93 0.9954976117766101 2316 94-95 1.5583409494439522 2316 96-97 1.5000996890180645 2316 98-99 -0.03204362127558369 2316 100-101 0.07179352219640833 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 13.0 4 54.0 5 164.0 6 349.0 7 885.0 8 1823.0 9 3253.0 10 5251.0 11 7781.0 12 10162.0 13 12502.0 14 15549.0 15 20243.0 16 24487.0 17 26960.0 18 22491.0 19 15965.0 20 14612.0 21 14281.0 22 15339.0 23 16927.0 24 20075.0 25 24849.0 26 31125.0 27 38451.0 28 46114.0 29 55472.0 30 64743.0 31 75193.0 32 87014.0 33 99756.0 34 116211.0 35 133653.0 36 134718.0 37 113006.0 38 48804.0 39 3108.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.735120973815945 21.0964437187706 16.19095415764936 18.977481149764092 2 16.045092452698796 22.924923624715014 39.093927218667964 21.936056703918226 3 19.235880398671096 28.910843884091754 31.2219708034721 20.63130491376505 4 12.622011669529815 19.06734574955161 37.049243599542905 31.26139898137567 5 12.002597266966806 40.26808154146887 32.56194807130981 15.16737312025452 6 27.915452667267044 38.261149244356325 17.44783901800377 16.375559070372862 7 25.658662469066662 37.26046057560599 19.942560485549308 17.138316469778037 8 27.078455263018487 33.928741703811895 19.037680018768118 19.9551230144015 9 28.015574508661334 16.62226897433763 20.392768221342678 34.96938829565836 10-11 24.76399851671346 28.819273643663113 25.01203278365963 21.404695055963796 12-13 27.093931390429777 27.02241578943385 26.685838397445117 19.197814422691255 14-15 23.986559607685844 26.791484724418986 27.92267990525129 21.299275762643884 16-17 19.74053041196076 30.547613569973393 28.33179959254965 21.3800564255162 18-19 19.473851898436255 27.09427362516526 33.9618443487362 19.47003012766228 20-21 21.677030126242112 25.468001675509 34.04200653932849 18.8129616589204 22-23 20.779784923451817 26.029597620687305 33.90656808360893 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20.299407684272005 27.77732746598105 31.332547518205434 20.590717331541512 50-51 20.52011211271873 28.243996666919173 30.865464737519886 20.37042648284221 52-53 20.585545585876925 28.4865295735927 30.33654765533733 20.59137718519304 54-55 20.10143539220549 28.47032853859581 30.51096327652598 20.917272792672723 56-57 20.596049728347378 28.728291732168266 29.81350166345357 20.862156876030788 58-59 20.55852259567569 28.10191934192261 30.18494045483514 21.154617607566557 60-61 20.645673260312787 28.233134434090783 30.40982477491387 20.711367530682555 62-63 20.927756251135193 28.16000181631047 30.322887025488892 20.589354907065445 64-65 20.570861821302667 28.268100487543496 30.427308187733765 20.73372950342007 66-67 20.26173520125278 28.361588103926483 30.584957920892723 20.791718773928014 68-69 20.539579364931004 28.20896473292851 30.579190585172515 20.67226531696797 70-71 20.11276623611185 28.392829118218586 30.696686241274385 20.79771840439518 72-73 20.71258417058065 28.46587757103037 29.66648225597267 21.155056002416313 74-75 20.190834140507405 28.876557694377368 29.85551205152575 21.077096113589477 76-77 20.23599757795943 28.8617544656373 29.804533757190434 21.097714199212835 78-79 20.149227467632507 28.75502877342015 29.82880505634156 21.266938702605785 80-81 19.92209582954622 29.240551640838618 29.549739020272188 21.287613509342975 82-83 20.13220926448664 29.153088792862064 29.16137552123143 21.553326421419868 84-85 20.037498391844952 28.8753887951324 29.01172250433256 22.07539030869009 86-87 20.142638108773667 28.86908140391982 29.09747795118156 21.890802536124948 88-89 20.261030332705452 28.595315954014705 29.088815547999474 22.054838165280362 90-91 19.85302798674073 28.723114414155326 29.423237016967622 22.000620582136314 92-93 19.928013616263836 28.94122045375942 29.25377697692001 21.876988953056735 94-95 19.49597856854637 28.774889338579957 29.490320349262305 22.238811743611368 96-97 19.49035295509727 28.554183420144224 29.40332968492464 22.552133939833872 98-99 19.741546249309817 28.520171192401474 28.94977950028137 22.78850305800734 100-101 19.777693095680217 28.44972854005751 29.290503197085556 22.482075167176717 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6548.0 1 4225.0 2 1956.5 3 2138.5 4 2039.0 5 2009.0 6 3096.5 7 5488.0 8 7904.5 9 9206.0 10 8357.0 11 6483.0 12 5513.0 13 5070.0 14 4972.0 15 4877.5 16 4748.5 17 4836.0 18 5060.5 19 5277.0 20 5676.5 21 6136.0 22 6617.5 23 7428.0 24 8359.0 25 9381.5 26 11014.5 27 12822.0 28 14344.5 29 16235.0 30 18460.5 31 21073.5 32 24256.0 33 27171.0 34 29743.5 35 32090.0 36 34858.0 37 37138.0 38 37640.5 39 37503.0 40 37560.0 41 38390.0 42 39384.5 43 40745.0 44 42007.0 45 42765.0 46 43113.0 47 44181.0 48 49347.5 49 49412.0 50 43155.5 51 38795.0 52 35857.5 53 34446.5 54 32956.5 55 30867.5 56 28578.0 57 26199.0 58 23995.0 59 21714.0 60 19207.0 61 16258.5 62 13047.5 63 9464.0 64 6376.5 65 4624.5 66 3420.0 67 2568.5 68 1934.0 69 1379.5 70 1079.0 71 922.5 72 808.5 73 722.5 74 552.0 75 416.0 76 286.5 77 148.0 78 79.0 79 53.0 80 34.0 81 19.5 82 15.0 83 9.5 84 7.0 85 4.5 86 1.5 87 0.5 88 0.0 89 0.5 90 0.5 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02414124520391406 2 0.021341163471798635 3 0.0 4 0.0 5 7.567788465176821E-5 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 1.5135576930353642E-4 18-19 9.838125004729868E-4 20-21 3.7838942325884105E-5 22-23 0.0 24-25 0.0 26-27 4.5406730791060923E-4 28-29 0.016270745200130166 30-31 0.004616350963757861 32-33 0.008892151446582766 34-35 0.010178675485662824 36-37 0.09932722360544578 38-39 0.10178675485662825 40-41 0.11461415630510297 42-43 0.10216514427988709 44-45 0.0964136250463527 46-47 0.09788934379706218 48-49 0.09966777408637872 50-51 0.097624471200781 52-53 0.0754886899401388 54-55 0.06394781253074414 56-57 0.03734703607564761 58-59 0.015741000007567788 60-61 0.008929990388908649 62-63 0.0022703365395530467 64-65 0.005448807694927312 66-67 0.42337992568431726 68-69 0.8155427239497801 70-71 0.9047669499542148 72-73 0.2784189376338553 74-75 0.14923678853328692 76-77 0.01437879808383596 78-79 0.008967829331234533 80-81 0.001740591346990669 82-83 0.0 84-85 0.0 86-87 3.0271153860707284E-4 88-89 8.324567311694504E-4 90-91 0.0037838942325884107 92-93 0.002194658654901278 94-95 0.22355247126132333 96-97 0.227563399147867 98-99 0.014113925487554774 100-101 0.008211050484716851 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1321390.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.73741873093381 #Duplication Level Percentage of deduplicated Percentage of total 1 71.00756964424619 31.76695376240587 2 14.005547662219156 12.53144100641499 3 5.6738515496611495 7.615004177830456 4 2.92273264451147 5.230220566243165 5 1.707018106444503 3.8183791904646736 6 1.0543505024630626 2.83013519507163 7 0.7338108233963245 2.298016145290088 8 0.5072876991606141 1.8155793771520303 9 0.38158268595575456 1.536392196186931 >10 1.8120791943771235 14.363240739261524 >50 0.11822426334223311 3.6432633128225174 >100 0.06977361038862277 5.591684732702347 >500 0.0037715417873100056 1.188772811409811 >1k 0.0017143371760500025 1.6457217411117244 >5k 1.7143371760500024E-4 0.609341934622642 >10k+ 5.143011528150008E-4 3.515853111009585 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18379 1.390883842014848 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16796 1.2710857506110989 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10667 0.8072559955804115 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7945 0.6012607935582985 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4571 0.34592361074323247 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3251 0.24602880300289848 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2782 0.21053587510121916 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2146 0.1624047404626946 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1859 0.1406851875676371 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1542 0.11669529813302659 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1495 0.11313843755439347 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1401 0.10602471639712727 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 3.7838942325884105E-5 0.0 14-15 0.0 0.0 0.0 1.1351682697765232E-4 0.0 16-17 0.0 0.0 0.0 4.540673079106093E-4 0.0 18-19 0.0 0.0 0.0 5.297451925623775E-4 0.0 20-21 0.0 0.0 0.0 9.081346158212186E-4 0.0 22-23 0.0 0.0 0.0 0.0018162692316424371 0.0 24-25 0.0 0.0 0.0 0.003140632213048381 0.0 26-27 0.0 0.0 0.0 0.004654189906083745 3.7838942325884105E-5 28-29 3.7838942325884105E-5 0.0 0.0 0.007302915868895633 7.567788465176821E-5 30-31 7.567788465176821E-5 0.0 0.0 0.01566532212291602 7.567788465176821E-5 32-33 7.567788465176821E-5 0.0 0.0 0.02959005289884137 7.567788465176821E-5 34-35 7.567788465176821E-5 0.0 0.0 0.053579942333451896 7.567788465176821E-5 36-37 7.567788465176821E-5 0.0 0.0 0.0869160505225558 7.567788465176821E-5 38-39 7.567788465176821E-5 0.0 0.0 0.13727968275830754 7.567788465176821E-5 40-41 7.567788465176821E-5 0.0 0.0 0.19733008422948561 1.5135576930353642E-4 42-43 1.5135576930353642E-4 0.0 0.0 0.2509100265629375 1.5135576930353642E-4 44-45 1.5135576930353642E-4 0.0 0.0 0.3102036491875979 1.5135576930353642E-4 46-47 1.5135576930353642E-4 0.0 0.0 0.3900816564375393 1.8919471162942054E-4 48-49 1.5135576930353642E-4 0.0 0.0 0.47820855311452337 2.2703365395530464E-4 50-51 1.5135576930353642E-4 0.0 0.0 0.5780276829702056 2.2703365395530464E-4 52-53 1.5135576930353642E-4 0.0 0.0 0.6759170267672678 2.2703365395530464E-4 54-55 1.5135576930353642E-4 0.0 0.0 0.7807687359522927 2.2703365395530464E-4 56-57 1.5135576930353642E-4 0.0 0.0 0.893604461968079 2.2703365395530464E-4 58-59 1.5135576930353642E-4 0.0 0.0 1.009542981254588 2.2703365395530464E-4 60-61 1.5135576930353642E-4 0.0 0.0 1.134297974103028 2.2703365395530464E-4 62-63 1.5135576930353642E-4 0.0 0.0 1.256025851565397 2.2703365395530464E-4 64-65 1.5135576930353642E-4 0.0 0.0 1.3761266545077533 2.2703365395530464E-4 66-67 1.5135576930353642E-4 0.0 0.0 1.5039844406269156 2.2703365395530464E-4 68-69 1.5135576930353642E-4 0.0 0.0 1.656664572911858 2.2703365395530464E-4 70-71 1.5135576930353642E-4 0.0 0.0 1.8647787557042206 2.2703365395530464E-4 72-73 1.5135576930353642E-4 0.0 0.0 2.088747455331129 2.2703365395530464E-4 74-75 1.5135576930353642E-4 0.0 0.0 2.328646349677234 2.2703365395530464E-4 76-77 1.5135576930353642E-4 0.0 0.0 2.588183655090473 2.2703365395530464E-4 78-79 1.5135576930353642E-4 0.0 0.0 2.884084184078887 2.2703365395530464E-4 80-81 1.5135576930353642E-4 0.0 0.0 3.2232724630881116 2.2703365395530464E-4 82-83 1.5135576930353642E-4 0.0 0.0 3.5664338310415546 2.2703365395530464E-4 84-85 1.5135576930353642E-4 0.0 0.0 3.900703047548415 2.2703365395530464E-4 86-87 1.5135576930353642E-4 0.0 0.0 4.248594283292594 2.2703365395530464E-4 88-89 1.5135576930353642E-4 0.0 0.0 4.62463769212723 2.2703365395530464E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11840 0.0 37.345562 1 GCGTATC 75 3.1199033E-4 31.59496 1 GTATCAA 17750 0.0 31.158857 1 CACGCGA 35 0.008317386 27.193598 90-91 CGTATCA 95 0.0012443411 24.93867 2 TATCAAC 22430 0.0 24.695173 2 ATCAACG 22445 0.0 24.61068 3 TCAACGC 22605 0.0 24.39457 4 CAACGCA 22890 0.0 24.028746 5 AACGCAG 23255 0.0 23.610857 6 GAGTACT 12980 0.0 23.213655 12-13 GTACATG 14740 0.0 22.795954 1 TACATGG 14565 0.0 22.642565 2 GAACCGT 105 0.002230421 22.559288 6 AGTACTT 13140 0.0 21.99356 12-13 GTACTTT 13810 0.0 21.698412 14-15 ACGCAGA 25970 0.0 21.069523 7 CGCAGAG 25930 0.0 21.010675 8 ACATGGG 14920 0.0 20.924799 3 ATAACGC 205 7.0066926E-7 20.798563 3 >>END_MODULE