FastQCFastQC Report
Fri 27 May 2016
SRR939452_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939452_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1312456
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127340.9702420500192006No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93940.7157573282456707No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65840.5016549126218327No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA48740.371364830516223No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24270.18492048495340033No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20860.15893866156274952No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18180.13851892939649024No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA14900.11352761540196395No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13200.10057480022187411No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA161250.034.987591
GGTATCA103900.033.6164631
TATCAAC199850.028.0236722
ATCAACG208250.027.1897093
TCAACGC210250.026.9637534
CAACGCA211150.026.6462465
AACGCAG214200.026.0579516
GTACATG125800.025.8440171
TACATGG128750.024.5039562
GAGTACT105800.024.1798512-13
ACGCAGA232300.023.61017
CGCAGAG233200.023.3318258
GTATAGG4550.023.2282261
AGTACTT108800.023.20374112-13
ACATGGG135650.022.6972523
GTACTTT116450.022.55121214-15
GCAGAGT226750.022.4565729
CAACGCG2204.4805347E-822.0658665
ATAACGC2004.7670983E-721.648393
ATGGGAG36300.021.5309375
Produced by FastQC (version 0.11.5)