FastQCFastQC Report
Fri 27 May 2016
SRR939452_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939452_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1312456
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181701.3844273636601911No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160891.2258696672497973No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103890.7915693935644319No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA79520.6058869783063203No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42260.3219917467709394No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32580.24823689327489834No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27230.2074736219728509No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA20070.15291941215553131No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA18940.14430959971229512No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15690.11954686480918218No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15390.11726107389504867No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14030.10689882175097679No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA110900.037.2824251
GTATCAA174200.033.163471
TATCAAC219550.026.2630042
ATCAACG221200.025.9967883
TCAACGC223950.025.5986714
CAACGCA227000.025.2268185
AACGCAG231100.024.7587496
AACGCGG1201.7344525E-423.7023495
GAGTACT134200.023.43206412-13
GTACATG140650.022.8827131
AGTACTT136150.022.53949712-13
TACATGG139950.022.3806462
GTACTTT142650.022.1104914-15
ACGCAGA258900.022.045297
CGCAGAG259550.022.0266118
AGTATCG1954.3048203E-721.8790915
ACGGAGC1302.9820358E-421.8790918
GCAGAGT263400.020.7867989
ACATGGG143800.020.720633
ACTTTTT161450.020.22568916-17
Produced by FastQC (version 0.11.5)