FastQCFastQC Report
Fri 27 May 2016
SRR939451_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939451_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2922938
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93210.31889147152625197No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73100.2500908332643388No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50340.1722239746446897No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA95600.035.8721581
GTATCAA144300.033.5412981
GTACATG146650.031.0664081
TACATGG149550.029.903112
ACATGGG156300.027.7506243
TATCAAC180950.026.5447372
TCAACGC185050.025.8272194
ATCAACG185400.025.718943
CAACGCA186250.025.3025635
AACGCAG188700.024.902986
CATGGGA98950.024.4644784
CATGGGG63950.024.0140694
GAGTACT108400.022.27832212-13
ACGCAGA209950.021.8167117
CGCAGAG214500.021.3059358
AGTACTT111250.020.6964412-13
ATGGGAG42900.020.5759535
GTACTTT118100.020.4009914-15
AGTACAT109150.019.9500452
GCAGAGT214300.019.9336649
Produced by FastQC (version 0.11.5)