FastQCFastQC Report
Fri 27 May 2016
SRR939451_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939451_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2922938
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132580.4535847151051442No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120800.4132827997035859No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80480.27533940165682613No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31280.10701561237357754No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA107150.038.131861
GTATCAA160650.032.307721
GTACATG168350.028.1834321
TACATGG171050.027.0933272
ATCAACG201850.025.5601843
ACATGGG174050.025.5325183
TCAACGC203750.025.321834
TATCAAC204850.025.2831272
CAACGCA209200.024.6621575
AACGCAG211850.024.3308816
CATGGGA109250.022.8102044
ACGCAGA242150.021.2276887
GAGTACT125000.021.11142212-13
CGCAGAG245700.020.8824168
CATGGGG68850.020.7122554
GTACTTT130950.020.00745814-15
GCAGAGT253200.019.7399569
AGTACTT127350.019.62437212-13
AGTACAT126400.018.3319382
AGAGTAC228000.018.09842310-11
Produced by FastQC (version 0.11.5)