##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939451_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2922938 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.97095969876884 31.0 31.0 31.0 30.0 34.0 2 31.22299822986324 31.0 31.0 34.0 30.0 34.0 3 31.214483851521997 31.0 31.0 34.0 28.0 34.0 4 35.157014962342686 35.0 35.0 37.0 33.0 37.0 5 34.78235870894285 35.0 35.0 37.0 32.0 37.0 6 34.741332864398764 35.0 35.0 37.0 32.0 37.0 7 34.59289523075755 35.0 35.0 37.0 30.0 37.0 8 34.611426243047234 35.0 35.0 37.0 30.0 37.0 9 35.95331580758812 37.0 35.0 39.0 30.0 39.0 10-11 35.810053275163554 37.0 35.0 39.0 30.0 39.0 12-13 35.76641174051588 37.0 35.0 39.0 30.0 39.0 14-15 36.62851076553796 38.0 35.5 40.0 30.0 41.0 16-17 36.63419357509465 38.0 35.0 40.0 30.5 41.0 18-19 36.57492666625156 38.0 35.0 40.0 30.0 41.0 20-21 36.465743542969435 38.0 35.0 40.0 30.0 41.0 22-23 36.32046882280774 38.0 34.5 40.0 30.0 41.0 24-25 36.09823711621663 38.0 34.0 40.0 29.0 41.0 26-27 35.7249645733163 38.0 34.0 40.0 27.5 41.0 28-29 35.43507850662587 38.0 34.0 40.0 27.0 41.0 30-31 35.200882639317015 38.0 33.5 40.0 27.0 41.0 32-33 34.99388953853965 37.5 33.0 40.0 26.5 41.0 34-35 34.95712755453588 38.0 33.0 40.0 26.0 41.0 36-37 34.95979251013877 38.0 33.0 40.0 25.5 41.0 38-39 34.91426366210984 38.0 33.0 40.0 26.0 41.0 40-41 34.6797987162232 38.0 33.0 40.0 25.0 41.0 42-43 34.36191924016178 37.0 33.0 40.0 24.5 41.0 44-45 34.15938808828651 37.0 33.0 40.0 24.0 41.0 46-47 33.82122097697591 37.0 32.5 40.0 23.5 41.0 48-49 33.48669626245921 37.0 32.0 40.0 22.5 41.0 50-51 33.202413633132146 37.0 31.5 40.0 21.5 41.0 52-53 32.80553453408864 36.0 31.0 39.0 20.0 41.0 54-55 32.56372338380082 36.0 31.0 39.0 19.5 40.0 56-57 32.14833414188053 35.5 30.5 39.0 18.0 40.0 58-59 31.690324769119293 35.0 30.0 39.0 15.0 40.0 60-61 31.375357602521845 35.0 29.0 38.0 13.5 40.0 62-63 30.968296282712807 34.5 29.0 38.0 11.5 40.0 64-65 30.500807577854886 34.0 28.5 38.0 8.0 40.0 66-67 28.757192249715867 33.0 25.0 36.5 4.5 39.5 68-69 25.015175484392756 29.5 15.5 35.0 2.0 38.0 70-71 24.404416549376002 28.5 13.0 34.0 2.0 37.0 72-73 26.289372884406035 31.0 21.0 35.0 2.0 37.0 74-75 26.926994003978187 31.0 23.5 35.0 2.0 37.0 76-77 27.006437016453994 31.5 25.0 34.0 2.0 36.0 78-79 27.86660373911455 32.5 26.0 35.0 2.0 36.0 80-81 27.743811021650135 33.0 26.0 35.0 2.0 36.0 82-83 27.359975134607716 32.5 26.0 35.0 2.0 36.0 84-85 27.04068953908704 32.0 25.5 35.0 2.0 35.5 86-87 26.607066759541254 32.0 25.0 34.0 2.0 35.0 88-89 26.13594130289455 32.0 24.0 34.0 2.0 35.0 90-91 25.694251126777235 31.5 23.0 34.0 2.0 35.0 92-93 25.12668862630682 31.0 20.0 34.0 2.0 35.0 94-95 23.717551655218138 31.0 10.0 34.0 2.0 35.0 96-97 22.91583160504944 30.0 3.5 34.0 2.0 35.0 98-99 22.59792920684599 30.0 2.0 34.0 2.0 35.0 100-101 21.352895613933654 28.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.32527966052583324 1101 2 0.22680226966506112 1101 3 0.2980367003786881 1101 4 0.2245992914213062 1101 5 0.26919868043608375 1101 6 0.4677600581113026 1101 7 0.47706964542805963 1101 8 0.36869438807435273 1101 9 0.6493081224478843 1101 10-11 0.6917082673671544 1101 12-13 0.5939817197174975 1101 14-15 0.7056569732270148 1101 16-17 0.7011004121845232 1101 18-19 0.687542002370094 1101 20-21 0.683537900051256 1101 22-23 0.7883136929193313 1101 24-25 0.6087609684874664 1101 26-27 0.701797885737065 1101 28-29 0.5483468836851202 1101 30-31 0.6756749915864191 1101 32-33 0.5774058879275046 1101 34-35 0.6946657763968744 1101 36-37 0.7722215305870037 1101 38-39 0.6758719324294233 1101 40-41 0.7447187624500771 1101 42-43 0.7488508250960493 1101 44-45 0.5419653272173335 1101 46-47 0.6331452460700646 1101 48-49 0.5123270767387353 1101 50-51 0.6077835978442891 1101 52-53 0.790055519876212 1101 54-55 0.5804893435218759 1101 56-57 0.23919666996837563 1101 58-59 0.3478162255448396 1101 60-61 0.18248923440463471 1101 62-63 0.20550288400152183 1101 64-65 -0.39896695932059956 1101 66-67 -3.126066090439469 1101 68-69 -7.916900602743834 1101 70-71 -11.17314206711538 1101 72-73 -11.118070109861922 1101 74-75 -9.823643136734958 1101 76-77 -4.939593752587317 1101 78-79 -2.007443944213172 1101 80-81 -1.3637955509570254 1101 82-83 -1.0079496773761214 1101 84-85 -1.198992784071784 1101 86-87 -1.178905304510483 1101 88-89 -1.2355207895973273 1101 90-91 -1.301911969851183 1101 92-93 -1.843292419859349 1101 94-95 -5.4096391308741865 1101 96-97 -2.4757834150313265 1101 98-99 -1.687775276015497 1101 100-101 -1.6820655312571802 1102 1 0.3665806219845926 1102 2 0.3044606859503567 1102 3 0.4067093555804533 1102 4 0.15896888123619135 1102 5 0.4276151342052614 1102 6 0.45311359822981245 1102 7 0.3837309163704674 1102 8 0.4730537210711816 1102 9 0.7734360907420523 1102 10-11 0.7264003370676946 1102 12-13 0.6970374276502298 1102 14-15 0.6743348865643171 1102 16-17 0.6888538506109327 1102 18-19 0.8010900156675689 1102 20-21 0.7962030570607652 1102 22-23 0.8592823489729113 1102 24-25 0.75482189883882 1102 26-27 0.8711411865590222 1102 28-29 0.8759003815498545 1102 30-31 0.5778187053093262 1102 32-33 0.4286320730044437 1102 34-35 0.6633891782505188 1102 36-37 0.9109771315711725 1102 38-39 0.7483794481431758 1102 40-41 0.9039706043980402 1102 42-43 1.0588359419274056 1102 44-45 0.7765851544148816 1102 46-47 0.868591869171091 1102 48-49 0.7220608105507225 1102 50-51 0.6871989659542095 1102 52-53 0.8570441413450425 1102 54-55 0.7702375292039747 1102 56-57 0.6220706479087958 1102 58-59 0.7418069229747708 1102 60-61 0.589610192076055 1102 62-63 0.4733877471754333 1102 64-65 0.4046096679384128 1102 66-67 -2.6666124479549467 1102 68-69 -7.486667603824099 1102 70-71 -11.12511065847418 1102 72-73 -9.539082249567603 1102 74-75 -7.807240104099815 1102 76-77 -4.2950011746958126 1102 78-79 -1.4013397054247427 1102 80-81 -0.7146766550947312 1102 82-83 -0.3644743479887218 1102 84-85 -0.3851527681946081 1102 86-87 -0.43736612643068895 1102 88-89 -0.38693196973117594 1102 90-91 -0.5777327680095361 1102 92-93 -0.9520008340892332 1102 94-95 -4.519019132505033 1102 96-97 -1.2125617144481957 1102 98-99 -0.6161910929454493 1102 100-101 -0.38354857482304894 1103 1 0.3322164478615264 1103 2 0.3132823012899806 1103 3 0.4417381535716167 1103 4 0.3035574514670287 1103 5 0.43362618907087125 1103 6 0.41948508992541633 1103 7 0.5359796876676413 1103 8 0.40748627831384 1103 9 0.6479113385773871 1103 10-11 0.6208441184352935 1103 12-13 0.5156773089573292 1103 14-15 0.7846413960954663 1103 16-17 0.6816519414193181 1103 18-19 0.6140202881189012 1103 20-21 0.7101993949195062 1103 22-23 0.6594894442269137 1103 24-25 0.886187985223323 1103 26-27 0.7171207699244064 1103 28-29 0.7609709869321222 1103 30-31 0.8022903003973809 1103 32-33 0.8401966708965176 1103 34-35 0.9461381125583586 1103 36-37 0.6957570940170754 1103 38-39 0.9255456593836939 1103 40-41 0.8891968981730258 1103 42-43 0.866869478089562 1103 44-45 0.6810769683607916 1103 46-47 0.7840185658302659 1103 48-49 0.7708561462398791 1103 50-51 0.7615917984169087 1103 52-53 0.8190825773049184 1103 54-55 0.860998611601488 1103 56-57 0.760182532354829 1103 58-59 0.9174456484033406 1103 60-61 0.7589281549619642 1103 62-63 0.7020759797850644 1103 64-65 0.5748655607088509 1103 66-67 -2.8194131573344023 1103 68-69 -7.492742739032366 1103 70-71 -11.515874559064768 1103 72-73 -9.093594071845637 1103 74-75 -7.233019844025883 1103 76-77 -3.7821687505792276 1103 78-79 -1.037770990622274 1103 80-81 -0.33538925870002245 1103 82-83 -0.007268752499907549 1103 84-85 -0.012895086039314663 1103 86-87 0.03616642743181586 1103 88-89 0.09460119104933895 1103 90-91 -0.013602435973549376 1103 92-93 0.21602608602916717 1103 94-95 0.8837092138958518 1103 96-97 1.0080546596058717 1103 98-99 0.7005796805135134 1103 100-101 0.632724644970093 1104 1 0.3930189850585535 1104 2 0.27956854925871966 1104 3 0.38320427365315624 1104 4 0.2160920330424716 1104 5 0.42944146849655596 1104 6 0.5145122789232701 1104 7 0.597033345090388 1104 8 0.4500424552198439 1104 9 0.626371074555621 1104 10-11 0.661029449701708 1104 12-13 0.6910168572149971 1104 14-15 0.7475228926543167 1104 16-17 0.7578373789515425 1104 18-19 0.779775442451907 1104 20-21 0.8927318099839994 1104 22-23 0.7169591107616142 1104 24-25 0.7636291247694089 1104 26-27 0.9181111521122318 1104 28-29 0.7995437934280503 1104 30-31 0.828355898851683 1104 32-33 0.863820804029416 1104 34-35 0.8797734767427912 1104 36-37 0.9624991920319772 1104 38-39 0.804024992323626 1104 40-41 0.8844033770916724 1104 42-43 1.0124481689704865 1104 44-45 0.8467596534488777 1104 46-47 0.9364020935877093 1104 48-49 0.8975953135379839 1104 50-51 0.9048598805654251 1104 52-53 1.2156315952247425 1104 54-55 1.0431464856842396 1104 56-57 1.1020134451285166 1104 58-59 1.224323307623191 1104 60-61 1.0044275164002983 1104 62-63 1.0608016846178288 1104 64-65 0.8376841048656125 1104 66-67 -2.375272484945917 1104 68-69 -6.815682592669713 1104 70-71 -8.408269584378981 1104 72-73 -7.840052095464724 1104 74-75 -6.740968467534788 1104 76-77 -3.392517047690685 1104 78-79 -0.3715786668303629 1104 80-81 0.32310808394020185 1104 82-83 0.7030456976069068 1104 84-85 0.6255753135108719 1104 86-87 0.758443725532878 1104 88-89 0.759073210984937 1104 90-91 0.8102299999994074 1104 92-93 0.9108440978480452 1104 94-95 1.8650971445382432 1104 96-97 1.9090314475049546 1104 98-99 1.2988404942994194 1104 100-101 1.2429227491511092 1105 1 0.44226158965529905 1105 2 0.3287692085929024 1105 3 0.4470506315573459 1105 4 0.2868831660406954 1105 5 0.4381558802085763 1105 6 0.5456640051408712 1105 7 0.5344283402270023 1105 8 0.5225352054913515 1105 9 0.7929085044673556 1105 10-11 0.8228253644598666 1105 12-13 0.7552084132935377 1105 14-15 0.9265067289907023 1105 16-17 0.909910495212479 1105 18-19 0.8521916709606359 1105 20-21 1.0051819574726082 1105 22-23 1.034929708266212 1105 24-25 0.9871440924262131 1105 26-27 1.0665083896199192 1105 28-29 1.0394695616498666 1105 30-31 0.8811627171107972 1105 32-33 0.9644083778020658 1105 34-35 1.1218979702820917 1105 36-37 1.0727517478588524 1105 38-39 1.0105441930509826 1105 40-41 0.9774192641432791 1105 42-43 1.0104875113594645 1105 44-45 0.9701280394178511 1105 46-47 0.9467467022610805 1105 48-49 0.8041877484264646 1105 50-51 0.7872039858956441 1105 52-53 1.0226958576343108 1105 54-55 1.0729741019916545 1105 56-57 1.1217532257888045 1105 58-59 1.1989594608118175 1105 60-61 1.1063630068537833 1105 62-63 1.2468255231308376 1105 64-65 1.1899126453291906 1105 66-67 1.7837329361958538 1105 68-69 -4.8844711227943485 1105 70-71 -5.580063209152531 1105 72-73 -1.7588817772929026 1105 74-75 1.0679663268540658 1105 76-77 1.2656840446999915 1105 78-79 1.1139207425349937 1105 80-81 1.071588985241867 1105 82-83 1.220820286033188 1105 84-85 1.1298140523415299 1105 86-87 1.3485087066801817 1105 88-89 1.4257914589880016 1105 90-91 1.4482236787349763 1105 92-93 1.5620822766527631 1105 94-95 2.269272058550456 1105 96-97 2.361028495465394 1105 98-99 2.035250796055905 1105 100-101 2.0844410769635395 1106 1 0.1558094621741759 1106 2 0.16014534803471392 1106 3 0.19107759180850792 1106 4 0.10810646362832443 1106 5 0.2427449236023307 1106 6 0.35206901278675673 1106 7 0.25854192132366194 1106 8 0.2065406512196688 1106 9 0.3056256653295364 1106 10-11 0.3815398284311513 1106 12-13 0.3545085419694374 1106 14-15 0.4296776320054434 1106 16-17 0.21896639942307417 1106 18-19 0.3939961853308773 1106 20-21 0.537916931667084 1106 22-23 0.5044736176974283 1106 24-25 0.6300124826355855 1106 26-27 0.6756794419809395 1106 28-29 0.7112700068749547 1106 30-31 0.4017557300855259 1106 32-33 0.4987940072263157 1106 34-35 0.7071719936601966 1106 36-37 0.567515429006697 1106 38-39 0.4851650645190233 1106 40-41 0.6999308898858203 1106 42-43 0.762629223668732 1106 44-45 0.5886548790015453 1106 46-47 0.5109664166200787 1106 48-49 0.31064778693183115 1106 50-51 0.24558155494936784 1106 52-53 0.5594515476736674 1106 54-55 0.3267507773485434 1106 56-57 0.521036742396003 1106 58-59 0.7769341844941344 1106 60-61 0.6610716354977555 1106 62-63 0.6604173337597459 1106 64-65 0.6346445092249375 1106 66-67 1.2598867715275048 1106 68-69 -5.506675614654434 1106 70-71 -6.256124893497255 1106 72-73 -2.532821824499319 1106 74-75 0.2700250870418124 1106 76-77 0.5745080833914962 1106 78-79 0.4567067217325693 1106 80-81 0.4910859263485641 1106 82-83 0.5469546955255531 1106 84-85 0.5874045772937215 1106 86-87 0.8011902841665552 1106 88-89 0.7511577632193713 1106 90-91 0.7438722366234352 1106 92-93 0.8146766272507442 1106 94-95 1.8142204204489722 1106 96-97 1.8204642045951083 1106 98-99 1.4929722695626317 1106 100-101 1.6005923907238504 1107 1 -0.05836936480368493 1107 2 -0.07722821273976876 1107 3 0.01084818801248133 1107 4 0.013597055155038618 1107 5 0.06605692727796963 1107 6 0.041705761910804995 1107 7 0.078328872897103 1107 8 0.008627709159412689 1107 9 0.22478802978206147 1107 10-11 0.18254000627229772 1107 12-13 0.20439350920418065 1107 14-15 0.1318211629680377 1107 16-17 0.08178536202641595 1107 18-19 0.3216083540265515 1107 20-21 0.14376549899311897 1107 22-23 0.14699759711297133 1107 24-25 0.4509409262022288 1107 26-27 0.4075914373096978 1107 28-29 0.42858436497181884 1107 30-31 0.30882837462092994 1107 32-33 0.38278631155499454 1107 34-35 0.3648320215507965 1107 36-37 0.3972135464848705 1107 38-39 0.3834280866829971 1107 40-41 0.40522630733688914 1107 42-43 0.4505832496651223 1107 44-45 0.36884595107533613 1107 46-47 0.12510678028078104 1107 48-49 0.4075939574630709 1107 50-51 0.37629846155117264 1107 52-53 0.5985837430492751 1107 54-55 0.5794872938503559 1107 56-57 0.7232672200066226 1107 58-59 0.6094543551765206 1107 60-61 0.5343324675461574 1107 62-63 0.5973375876101201 1107 64-65 0.6070620245848879 1107 66-67 1.3351001339019604 1107 68-69 -5.470841376135997 1107 70-71 -6.100547919300762 1107 72-73 -2.2556695612733684 1107 74-75 0.3639372425403913 1107 76-77 0.49217195761382015 1107 78-79 0.5453614719146422 1107 80-81 0.41546546566856435 1107 82-83 0.4605865011921786 1107 84-85 0.5608531389047755 1107 86-87 0.6996382911989905 1107 88-89 0.5896477377538751 1107 90-91 0.5654051708857537 1107 92-93 0.8326078459663968 1107 94-95 1.7138643433764535 1107 96-97 1.8339519326162161 1107 98-99 1.1873567756511534 1107 100-101 1.189953617669829 1108 1 0.06406905418268494 1108 2 -0.019218917201087038 1108 3 0.06290802997288125 1108 4 -0.019721351787282515 1108 5 0.0037625350973371496 1108 6 0.08505740580828558 1108 7 0.10248742977244518 1108 8 0.0033009177029725834 1108 9 0.26789396726879033 1108 10-11 0.21148829404797453 1108 12-13 0.32124331368132886 1108 14-15 0.21447724347790142 1108 16-17 0.039006003681087975 1108 18-19 0.16320077476548533 1108 20-21 0.24238255599777858 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0.7220224971480995 2311 62-63 0.9584066230790782 2311 64-65 0.9388774736138394 2311 66-67 2.034976538847353 2311 68-69 4.480087704033611 2311 70-71 4.832846939550773 2311 72-73 2.858620686847452 2311 74-75 1.7977935979154331 2311 76-77 1.1673070762972735 2311 78-79 1.0067986680271765 2311 80-81 0.8410715644360991 2311 82-83 0.7166461655799985 2311 84-85 0.668684200079916 2311 86-87 0.8483238697027993 2311 88-89 1.1727625448560453 2311 90-91 0.9979780476378401 2311 92-93 0.9896996084574603 2311 94-95 1.680092501964527 2311 96-97 1.9652352877323302 2311 98-99 1.7067212138869294 2311 100-101 1.4678718967255833 2312 1 -0.20488256738666166 2312 2 -0.15556936530872179 2312 3 -0.05960803527674585 2312 4 -0.13200401421286045 2312 5 0.10451260494625103 2312 6 0.26024492327051973 2312 7 0.1503231881167011 2312 8 0.21301743858241906 2312 9 -1.502433140556434 2312 10-11 -0.9689964573973597 2312 12-13 -1.8960421427007148 2312 14-15 -2.054988573539447 2312 16-17 0.09419094210658585 2312 18-19 -1.9982077016685622 2312 20-21 -1.7356397526699396 2312 22-23 -0.6211195682264545 2312 24-25 -1.1021284681185861 2312 26-27 -1.3413650933521382 2312 28-29 -0.044434356646902984 2312 30-31 0.47362179902831514 2312 32-33 0.39155971818668434 2312 34-35 0.8010779343860079 2312 36-37 0.7296937838545645 2312 38-39 0.5500335655652222 2312 40-41 0.9154577753490827 2312 42-43 0.6796890549675467 2312 44-45 0.7476036618882418 2312 46-47 0.7767599871709692 2312 48-49 0.7583968128793614 2312 50-51 0.6212365676750693 2312 52-53 0.8584497740593449 2312 54-55 0.7141521348169064 2312 56-57 0.7479907459468116 2312 58-59 1.0382614975267508 2312 60-61 0.9694697038101694 2312 62-63 0.37112785902888845 2312 64-65 1.1032518945298655 2312 66-67 2.2543114443538244 2312 68-69 4.346083595146705 2312 70-71 5.126557890302717 2312 72-73 3.1502202724504933 2312 74-75 2.2791505585179017 2312 76-77 1.154881131511143 2312 78-79 1.065738847447168 2312 80-81 1.1038586857138668 2312 82-83 1.2660777029008088 2312 84-85 1.2489150232570978 2312 86-87 1.2317720930617817 2312 88-89 1.0179842973987512 2312 90-91 1.129160717340799 2312 92-93 1.3276468735322382 2312 94-95 1.9985380783330946 2312 96-97 2.47866030037088 2312 98-99 2.2203244466884158 2312 100-101 1.9503158073671152 2313 1 -0.2464377389556489 2313 2 -0.11308551279996237 2313 3 -0.09443497728256034 2313 4 0.15642597609993913 2313 5 0.10527290700670733 2313 6 0.08259849640884909 2313 7 -3.444520652376127 2313 8 -1.0320424376396247 2313 9 -0.5738645255138408 2313 10-11 0.09904689778338138 2313 12-13 -1.1636997414360977 2313 14-15 -0.1425897415434747 2313 16-17 0.01656308453520694 2313 18-19 0.38720084003183075 2313 20-21 0.3432342817486074 2313 22-23 -0.8978543684615801 2313 24-25 -2.691193428603647 2313 26-27 -2.010660100940015 2313 28-29 -0.29034900457056523 2313 30-31 0.20993657836541502 2313 32-33 -2.2410844232238745 2313 34-35 -2.355662999341966 2313 36-37 -0.875448221438333 2313 38-39 -0.12048175604709854 2313 40-41 -0.08823606336905243 2313 42-43 -0.21086997589730316 2313 44-45 0.34256686093112165 2313 46-47 0.3626937064915907 2313 48-49 -0.09811386597753113 2313 50-51 0.05841111813808908 2313 52-53 0.6129552076328011 2313 54-55 0.6531759312109102 2313 56-57 0.4936017491819449 2313 58-59 0.44679172737906825 2313 60-61 1.0238907098761345 2313 62-63 0.7034070208223824 2313 64-65 0.41475931402182553 2313 66-67 1.9198255020424213 2313 68-69 4.942882080689063 2313 70-71 4.898144582051632 2313 72-73 3.246946796582346 2313 74-75 2.255208185648282 2313 76-77 1.3370622093610507 2313 78-79 0.9926056021845007 2313 80-81 0.9988261916201147 2313 82-83 0.854781016718352 2313 84-85 0.896176745500334 2313 86-87 1.0874614379251 2313 88-89 1.2095067238206454 2313 90-91 1.1854183084580114 2313 92-93 1.3148215105653769 2313 94-95 2.2728771587679866 2313 96-97 2.2251289970490973 2313 98-99 1.963274500924598 2313 100-101 1.6354935098481285 2314 1 -0.23398538498428678 2314 2 -0.22981141506980052 2314 3 -0.21986507927081433 2314 4 -0.07609694550302493 2314 5 -1.3019340913793371 2314 6 -2.8430299160769863 2314 7 -0.7680612244718645 2314 8 -0.2189771239484699 2314 9 0.09103365792766738 2314 10-11 0.3652637172607385 2314 12-13 0.05831401152568105 2314 14-15 -2.583800980855308 2314 16-17 -2.7983737308214103 2314 18-19 -0.3979275896237482 2314 20-21 0.13046257061231614 2314 22-23 0.048646867759700285 2314 24-25 -2.34813687147993 2314 26-27 -1.1310792142946369 2314 28-29 -0.12036943656121935 2314 30-31 0.18536796808417222 2314 32-33 0.19548540610891507 2314 34-35 0.06286529638963145 2314 36-37 0.286099287232382 2314 38-39 -1.111076172447632 2314 40-41 -3.6660836647563677 2314 42-43 -5.2105523357063745 2314 44-45 -2.0622805859285265 2314 46-47 -2.9103322026461385 2314 48-49 -0.6332719134347045 2314 50-51 -0.31798735718899707 2314 52-53 -2.098435627159965 2314 54-55 -1.0669238249787796 2314 56-57 -3.529021529551624 2314 58-59 -4.63392708378813 2314 60-61 -2.1654928106073648 2314 62-63 -1.014065335896138 2314 64-65 -0.09601899029464889 2314 66-67 -0.11650953109517559 2314 68-69 1.9084661952455697 2314 70-71 1.9362588295019272 2314 72-73 -0.23537867301004312 2314 74-75 0.3287427483350065 2314 76-77 0.30609014452811323 2314 78-79 0.6376246605959572 2314 80-81 0.9863352057336421 2314 82-83 0.6654206753839205 2314 84-85 0.43875684233766776 2314 86-87 0.3658983906396358 2314 88-89 0.6069960785818154 2314 90-91 0.8821204306378831 2314 92-93 1.094058143922748 2314 94-95 1.1477183386725223 2314 96-97 1.4436195193525876 2314 98-99 1.0408117592605066 2314 100-101 1.0020438515259578 2315 1 -0.37084813008232587 2315 2 -0.2778179510175143 2315 3 -0.462250700185848 2315 4 -0.2201492330847259 2315 5 -1.3601367057584284 2315 6 -0.37159200758025435 2315 7 -0.17528344669408824 2315 8 -0.029173202379844554 2315 9 0.0847918278623041 2315 10-11 -1.7123833415627914 2315 12-13 -1.2335323937030793 2315 14-15 -0.15800032726053814 2315 16-17 -1.7718704628475521 2315 18-19 -2.9268164785126345 2315 20-21 -2.086106056838659 2315 22-23 -1.6633792760311508 2315 24-25 -1.096110727689073 2315 26-27 -0.34959228619005955 2315 28-29 -0.41592499211677847 2315 30-31 -2.961069940412557 2315 32-33 -2.5415407376819346 2315 34-35 -1.5332948343293253 2315 36-37 -4.901319013421208 2315 38-39 -2.19035721819926 2315 40-41 -1.4709856255406777 2315 42-43 -1.3784118128305565 2315 44-45 -1.0406302591311416 2315 46-47 -1.1076361242147712 2315 48-49 -4.5014418480752205 2315 50-51 -3.935634416012526 2315 52-53 -2.4292689604932995 2315 54-55 -3.6850610798807395 2315 56-57 -6.257648980532014 2315 58-59 -7.224188521696629 2315 60-61 -5.7832215687773 2315 62-63 -6.787643767268687 2315 64-65 -6.015300036046284 2315 66-67 -4.164973583382761 2315 68-69 -1.9538867459308982 2315 70-71 0.5249353000901635 2315 72-73 -1.2404276926178888 2315 74-75 -3.0925807810767587 2315 76-77 -5.622488286844437 2315 78-79 -4.348796908031492 2315 80-81 -3.9762628334820462 2315 82-83 -7.085020501086671 2315 84-85 -5.020066687074095 2315 86-87 -5.557157164915921 2315 88-89 -3.984507189392044 2315 90-91 -3.643042968054928 2315 92-93 -4.6717425096720255 2315 94-95 -2.834324144987608 2315 96-97 -1.474828193065715 2315 98-99 -3.0936490250532174 2315 100-101 -2.3883973249446306 2316 1 -0.40712075121484403 2316 2 -0.3603125997818637 2316 3 -0.8861746153444372 2316 4 -0.2697269719444293 2316 5 -0.3070154759471748 2316 6 -2.224902963019055 2316 7 -0.5339835829073607 2316 8 -0.391207644878385 2316 9 -0.05970807484250429 2316 10-11 0.004694883772877745 2316 12-13 -0.6604160286515111 2316 14-15 -1.0658238337793193 2316 16-17 -1.5759646445949755 2316 18-19 -0.3364564862962496 2316 20-21 -4.143338981532182 2316 22-23 -4.6383680870838795 2316 24-25 -2.9507526813765637 2316 26-27 -2.310864420846613 2316 28-29 -1.4300892264036094 2316 30-31 -0.349608080128462 2316 32-33 -2.157532175704894 2316 34-35 -2.640470354858607 2316 36-37 -5.06750893363915 2316 38-39 -2.2860538517855673 2316 40-41 -1.7333761684478617 2316 42-43 -3.160884570176343 2316 44-45 -0.7018727187536413 2316 46-47 -0.648033088604727 2316 48-49 -0.13708059102131642 2316 50-51 0.07898905158817371 2316 52-53 -2.769779760259791 2316 54-55 -0.6701603209782299 2316 56-57 0.07861342460517662 2316 58-59 0.38739301167562346 2316 60-61 -0.8792032772809009 2316 62-63 -0.3778101420497748 2316 64-65 0.6462245796376997 2316 66-67 1.7620613455917535 2316 68-69 4.154426155411773 2316 70-71 4.395663554614391 2316 72-73 2.626398051690341 2316 74-75 1.6265596158876292 2316 76-77 0.22417546096642127 2316 78-79 0.549888147426767 2316 80-81 0.23817236791900243 2316 82-83 0.4953968952939505 2316 84-85 0.7581878371524091 2316 86-87 0.24858227855557047 2316 88-89 0.7559895025713743 2316 90-91 0.7881620329180237 2316 92-93 0.954050932631926 2316 94-95 1.5670248528718993 2316 96-97 1.448640380586749 2316 98-99 0.7010882779240148 2316 100-101 0.6367340235667527 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 39.0 4 114.0 5 238.0 6 626.0 7 1457.0 8 3145.0 9 5041.0 10 7862.0 11 11483.0 12 15289.0 13 19097.0 14 23299.0 15 28673.0 16 34132.0 17 38644.0 18 35960.0 19 31605.0 20 31119.0 21 31398.0 22 32535.0 23 37502.0 24 45838.0 25 56711.0 26 71851.0 27 89427.0 28 109115.0 29 129903.0 30 153018.0 31 177483.0 32 204326.0 33 235848.0 34 273324.0 35 310883.0 36 320782.0 37 254118.0 38 96039.0 39 5007.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.18422217028314 19.336793479767316 14.079672797493146 23.399311552456396 2 16.843079586847136 20.746474222429814 37.57432662481757 24.836119565905477 3 19.849685487683967 24.301678653464425 29.76108285567467 26.08755300317694 4 13.699572142823419 16.233289929516122 34.91880429896221 35.148333628698246 5 14.07524565222672 37.78341823093447 32.989979585573806 15.151356531265 6 33.589114787929134 35.05739772790254 16.109099816691288 15.244387667477039 7 29.67924738738899 32.77941577960258 19.3885056747697 18.15283115823873 8 27.784236271860713 34.385778966231925 18.612266151386038 19.21771861052133 9 27.302323894656677 15.756817284526733 19.478791544671832 37.46206727614476 10-11 26.388431776520747 26.13972653542429 25.74105232474996 21.730789363305004 12-13 26.796001146791344 23.41607998527509 26.985040394288212 22.802878473645354 14-15 23.584677471776686 24.63298913627316 25.327273448838124 26.455059943112037 16-17 23.03884211777767 28.056303237598673 25.911685992358674 22.993168652264984 18-19 22.81063536542454 25.971049042417853 28.761112433044005 22.457203159113604 20-21 23.59619389740629 25.149716680565355 28.739718177347275 22.51437124468108 22-23 23.382329697037708 25.131186497968827 28.68976351876092 22.79672028623255 24-25 23.248816772712935 25.397339936734888 28.762053796556753 22.591789493995424 26-27 23.028853465082662 25.739460598451995 28.703820437373594 22.52786549909175 28-29 22.68176186970464 25.760227919954954 28.97645606164323 22.581554148697172 30-31 22.743923165470925 25.61101929467988 28.84557122526837 22.79948631458083 32-33 22.687228437803338 25.598809719732586 28.65809825457723 23.05586358788684 34-35 23.24249771268599 25.57229041394738 28.48354331457633 22.7016685587903 36-37 23.223528138580026 25.48189888967619 28.405741313491045 22.888831658252737 38-39 23.115593120381003 25.591507296041506 28.604619861630876 22.688279721946618 40-41 23.01209224738027 25.55166115332346 28.75317377606778 22.683072823228486 42-43 23.05507151673564 25.66377818784085 28.285242250013358 22.99590804541015 44-45 22.92500703758929 25.852644181540352 28.132754618610605 23.089594162259754 46-47 23.095956338199162 26.052679299018028 27.617530816463553 23.23383354631926 48-49 23.342487699997054 26.181163306640805 27.335271058981792 23.14107793438035 50-51 23.09328248376576 26.343469856271547 27.279056693945915 23.284190966016784 52-53 23.548582702076402 26.253908406000352 27.073646489727555 23.12386240219569 54-55 22.97781682136509 26.11932684960246 27.304351458017795 23.598504871014654 56-57 23.18175743212591 26.600955464315028 27.04803897426455 23.169248129294512 58-59 23.33053092469904 26.062964886743682 27.07213995208189 23.534364236475383 60-61 23.245412869541298 26.081099737721846 27.445284869727427 23.22820252300943 62-63 23.26089496456486 26.186028108278986 27.290676595701736 23.26240033145442 64-65 23.06296116102924 26.16081026801141 27.249514675381644 23.526713895577714 66-67 22.43902246257812 26.35037828219986 27.78988491575459 23.420714339467423 68-69 22.279085895275077 26.03017918784966 28.493127998468097 23.197606918407164 70-71 21.708287307700346 26.125228516512333 28.72723015452698 23.439254021260343 72-73 22.801041232583863 26.31064628235259 27.203822496367643 23.684489988695905 74-75 22.71245715836516 26.50236130138512 27.09996826926306 23.68521327098666 76-77 22.96061198278666 26.48127861740314 26.89830164271999 23.659807757090203 78-79 22.81525494594378 26.64382361776687 26.88904224795035 23.65187918833899 80-81 22.677160586116727 26.834471529806535 26.857000532690318 23.631367351386416 82-83 22.84348829841755 26.81644290778662 26.556875308337023 23.78319348545881 84-85 22.741536084583387 26.571432579137838 26.473620035731173 24.213411300547598 86-87 22.8792791350332 26.37746058496722 26.819858683041996 23.92340159695758 88-89 22.68193746631152 26.492424272230465 26.521898217895423 24.303740043562595 90-91 22.622003613958853 26.701452545962677 26.684209004720934 23.99233483535754 92-93 22.659049974194957 26.620966283750764 26.67678454646678 24.0431991955875 94-95 22.13113151936069 26.421013250045178 27.07676000577475 24.371095224819374 96-97 22.120444882486527 26.25251799893416 27.057111679468427 24.569925439110893 98-99 22.360490621759556 26.307934905175696 26.47458808469971 24.856986388365037 100-101 22.323832307547818 26.13671958598832 26.77443045766109 24.76501764880277 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1851.0 1 1281.0 2 802.5 3 1003.5 4 1035.5 5 1096.0 6 1590.5 7 2818.0 8 4201.5 9 4889.0 10 4719.0 11 4315.5 12 4175.0 13 4193.0 14 4353.0 15 4435.5 16 4629.0 17 4934.5 18 5183.5 19 5483.5 20 6049.5 21 6764.5 22 7534.5 23 8651.0 24 9870.0 25 11583.5 26 13993.0 27 16652.5 28 19493.0 29 22550.0 30 25849.0 31 29476.5 32 33820.5 33 37493.0 34 41014.0 35 44855.5 36 49973.0 37 56460.0 38 61659.0 39 64303.5 40 66345.5 41 72378.0 42 79110.5 43 84352.5 44 89879.5 45 95345.0 46 100541.5 47 107184.5 48 117376.0 49 121167.5 50 118553.5 51 117087.0 52 116511.5 53 118123.5 54 116679.0 55 109691.0 56 101895.0 57 93554.0 58 85845.0 59 77689.0 60 67031.0 61 54923.5 62 43242.5 63 33436.5 64 25476.5 65 19659.5 66 14902.0 67 11398.0 68 8174.5 69 5363.5 70 3852.0 71 3010.0 72 2418.0 73 2000.0 74 1571.0 75 1138.5 76 738.5 77 445.5 78 298.0 79 184.5 80 91.5 81 59.5 82 46.0 83 27.5 84 13.5 85 6.0 86 5.0 87 5.0 88 1.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024324840280567018 2 0.018235077172351927 3 0.0 4 0.0 5 1.7106076146671602E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 5.302883605468197E-4 18-19 0.0010092584926536246 20-21 1.7106076146671605E-5 22-23 0.0 24-25 0.0 26-27 4.6186405596013326E-4 28-29 0.017396879441165018 30-31 0.00470417094033469 32-33 0.00906622035773595 34-35 0.010126797078829589 36-37 0.1019180016818694 38-39 0.10492867108368362 40-41 0.11993069986431461 42-43 0.10475761032221691 44-45 0.09935209025986867 46-47 0.10087453103692244 48-49 0.10332069992589647 50-51 0.10101137964609581 52-53 0.07903007179762281 54-55 0.0679453344545796 56-57 0.038061019426344316 58-59 0.01546389283659113 60-61 0.009493872261402738 62-63 0.0021382595183339504 64-65 0.005200247148588167 66-67 0.43822345872543306 68-69 0.8410373398272559 70-71 0.9360273806697234 72-73 0.2910598856356173 74-75 0.1584706894227657 76-77 0.014967816628337651 78-79 0.008210916550402368 80-81 0.001334273939440385 82-83 0.0 84-85 0.0 86-87 1.7106076146671602E-4 88-89 5.98712665133506E-4 90-91 0.003575169914654365 92-93 0.002035623061453921 94-95 0.23253999913785375 96-97 0.2371586396974551 98-99 0.013736179145777298 100-101 0.007475355276095491 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2922938.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.253382506636164 #Duplication Level Percentage of deduplicated Percentage of total 1 65.02905806273976 23.575233159947572 2 14.393394466516 10.436184703270095 3 6.637689395731114 7.219160778710481 4 3.8103871073915037 5.525576852104765 5 2.3775355806304024 4.309685341386564 6 1.6328986706061093 3.551886006003658 7 1.1295870766146532 2.8665946654144765 8 0.8557768154491295 2.481984338463062 9 0.654836649624103 2.136603918436802 >10 3.149983120873233 19.899858089345486 >50 0.19368667381494267 4.83684688060044 >100 0.12425675709221536 8.577803632364398 >500 0.007751574177758633 1.9428597391228677 >1k 0.002870953399157956 1.4814305510765695 >5k 9.569844663859853E-5 0.2792166996502162 >10k+ 1.9139689327719707E-4 0.8790746441025321 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13258 0.4535847151051442 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12080 0.4132827997035859 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8048 0.27533940165682613 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3128 0.10701561237357754 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.0263645688002962E-4 2 0.0 0.0 0.0 0.0 1.0263645688002962E-4 3 0.0 0.0 0.0 0.0 1.0263645688002962E-4 4 0.0 0.0 0.0 0.0 1.0263645688002962E-4 5 0.0 0.0 0.0 0.0 1.368486091733728E-4 6 0.0 0.0 0.0 0.0 1.7106076146671602E-4 7 0.0 0.0 0.0 0.0 1.7106076146671602E-4 8 0.0 0.0 0.0 0.0 1.7106076146671602E-4 9 0.0 0.0 0.0 0.0 1.7106076146671602E-4 10-11 0.0 0.0 0.0 1.71060761466716E-5 1.7106076146671602E-4 12-13 0.0 0.0 0.0 3.42121522933432E-5 5.302883605468197E-4 14-15 0.0 0.0 0.0 5.13182284400148E-5 6.671369697201925E-4 16-17 0.0 0.0 0.0 2.052729137600592E-4 7.69773426600222E-4 18-19 0.0 0.0 0.0 2.736972183467456E-4 8.553038073335802E-4 20-21 0.0 0.0 0.0 4.4475797981346165E-4 0.001077682797240311 22-23 0.0 0.0 0.0 0.0011461071018269973 0.0011290010256803258 24-25 0.0 0.0 0.0 0.0017790319192538466 0.0011290010256803258 26-27 0.0 0.0 0.0 0.0026001235742940834 0.0013513800155870565 28-29 0.0 0.0 0.0 0.004362049417401259 0.0014026982440270713 30-31 0.0 0.0 0.0 0.010862358353136467 0.0014026982440270713 32-33 0.0 0.0 0.0 0.024051143062220275 0.0014369103963204144 34-35 0.0 0.0 0.0 0.04449290405749284 0.0015395468532004442 36-37 0.0 0.0 0.0 0.0761391449288353 0.0015395468532004442 38-39 0.0 0.0 0.0 0.12128207987990165 0.001590865081640459 40-41 0.0 0.0 0.0 0.17557676556943733 0.0016079711577871307 42-43 0.0 0.0 0.0 0.22846875301494593 0.0016421833100804739 44-45 0.0 0.0 0.0 0.2931126147732179 0.0017106076146671603 46-47 0.0 0.0 0.0 0.37356249089101445 0.001847456223840533 48-49 0.0 0.0 0.0 0.4583573103500656 0.0020014109091605775 50-51 0.0 0.0 0.0 0.5530736539741863 0.0020869412898939354 52-53 0.0 0.0 0.0 0.650646712314801 0.0022922142036539946 54-55 0.0 0.0 0.0 0.7561740960636182 0.0024290628128273675 56-57 0.0 0.0 0.0 0.8696215930683442 0.002446168888974039 58-59 0.0 0.0 0.0 0.9827611807024302 0.002463274965120711 60-61 0.0 1.71060761466716E-5 0.0 1.1015457734649177 0.0025145931935607255 62-63 0.0 3.42121522933432E-5 0.0 1.2174565454347646 0.0026001235742940834 64-65 0.0 1.0263645688002962E-4 0.0 1.3384991402486128 0.0026001235742940834 66-67 0.0 1.0263645688002962E-4 0.0 1.4660420439981965 0.0026001235742940834 68-69 0.0 1.0263645688002962E-4 0.0 1.6192440619677873 0.0026001235742940834 70-71 0.0 1.0263645688002962E-4 0.0 1.8245340818039932 0.002617229650440755 72-73 0.0 1.0263645688002962E-4 0.0 2.047751269441911 0.002754078259614128 74-75 0.0 1.0263645688002962E-4 0.0 2.288040321074207 0.0028909268687875005 76-77 0.0 1.0263645688002962E-4 0.0 2.5494211645953486 0.0029593511733741872 78-79 0.0 1.0263645688002962E-4 0.0 2.8518565908685027 0.0030277754779608735 80-81 0.0 1.0263645688002962E-4 0.0 3.184586877997412 0.003079093706400888 82-83 0.0 1.0263645688002962E-4 0.0 3.5314981022519123 0.003352790924747634 84-85 0.0 1.0263645688002962E-4 0.0 3.873893322403691 0.003352790924747634 86-87 0.0 1.0263645688002962E-4 0.0 4.224140231506793 0.003489639533921007 88-89 0.0 1.0263645688002962E-4 0.0 4.608428232141769 0.00362648814309438 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10715 0.0 38.13186 1 GTATCAA 16065 0.0 32.30772 1 GTACATG 16835 0.0 28.183432 1 TACATGG 17105 0.0 27.093327 2 ATCAACG 20185 0.0 25.560184 3 ACATGGG 17405 0.0 25.532518 3 TCAACGC 20375 0.0 25.32183 4 TATCAAC 20485 0.0 25.283127 2 CAACGCA 20920 0.0 24.662157 5 AACGCAG 21185 0.0 24.330881 6 CATGGGA 10925 0.0 22.810204 4 ACGCAGA 24215 0.0 21.227688 7 GAGTACT 12500 0.0 21.111422 12-13 CGCAGAG 24570 0.0 20.882416 8 CATGGGG 6885 0.0 20.712255 4 GTACTTT 13095 0.0 20.007458 14-15 GCAGAGT 25320 0.0 19.739956 9 AGTACTT 12735 0.0 19.624372 12-13 AGTACAT 12640 0.0 18.331938 2 AGAGTAC 22800 0.0 18.098423 10-11 >>END_MODULE