FastQCFastQC Report
Fri 27 May 2016
SRR939450_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939450_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2895290
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92450.31931170970783584No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67070.23165209702655No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49100.16958577551816914No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA137350.036.0422551
GGTATCA87350.035.621511
GTACATG139850.030.131251
TACATGG141700.028.8980032
TATCAAC173350.028.0984522
ATCAACG175450.028.001783
TCAACGC178800.027.8011884
CAACGCA180150.027.084845
AACGCAG180600.026.808166
ACATGGG150650.026.197643
ACGCAGA202650.023.4858917
CATGGGA92200.023.3643684
CGCAGAG206750.022.9503028
CATGGGG65750.021.455694
GAGTACT103150.021.32491912-13
AGTACAT101650.021.3182372
GCAGAGT203600.021.2839939
GAGTACA120600.020.814891
AGTACTT106200.020.50897412-13
GTACTTT113450.019.88650314-15
Produced by FastQC (version 0.11.5)