FastQCFastQC Report
Fri 27 May 2016
SRR939450_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939450_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2895290
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129200.44624199993783004No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114440.39526265071892625No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78900.27251156188153863No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29400.10154423218399539No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA104750.035.5635221
GTATCAA155050.031.647391
GTACATG162000.025.9835071
TACATGG164700.025.2061182
ATCAACG199350.024.4603863
TATCAAC200200.024.4098242
TCAACGC199450.024.3826734
CAACGCA202700.023.9279635
AACGCAG205550.023.6423246
ACATGGG168950.023.4430833
CGTATAG1805.215121E-621.0914571
AGTACTT123000.020.9257512-13
ACGCAGA234900.020.6479267
CATGGGG67250.020.4596044
CGCAGAG239300.020.1890248
AGTACAT124900.020.0188752
GAGTACT121650.019.98902312-13
CATGGGA106750.019.9558374
GAGTACA145050.019.3356551
GCAGAGT243500.019.1398439
Produced by FastQC (version 0.11.5)