FastQCFastQC Report
Fri 27 May 2016
SRR939449_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939449_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1786522
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92830.5196129686620148No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75990.4253516049620436No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT61530.3444122154667001No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54240.3036066726298361No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA42910.24018735845402406No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37040.20733022039471105No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT36370.20357991673206374No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA34080.19076171466122444No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT27160.15202723504104623No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC21780.12191285637680364No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT20490.11469212245916927No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC18940.1060160468217016No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG18170.1017059963437338No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTCCG5950.038.74829
ATTGCCG1251.640201E-731.3613268
GTACATG81350.030.1976871
GACATAT8250.029.7161061
GGCATTC7800.028.6355937
TACATGG86600.028.2277582
CATGGGG14600.028.1973154
ACATGGG83950.027.3321633
TACCTGG3600.027.1071622
GTGGTAC5300.026.4321171
ACCGGTC459.3539985E-426.19068578-79
ACTCCCG5250.025.883076
GACAATG5550.025.3716077
TAGGCAT8650.025.128915
CATGGGA58300.025.0434074
GGTACTC5450.024.6105183
CGCGGAG2001.4768375E-824.5010368
CCTTTAG9250.024.3399981
GTATGGT4850.023.726621
CTTTAGG9050.023.7225112
Produced by FastQC (version 0.11.5)