FastQCFastQC Report
Fri 27 May 2016
SRR939449_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939449_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1786522
Sequences flagged as poor quality0
Sequence length101
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123980.6939741016343487No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115080.6441566350708248No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT88930.4977828428645155No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83680.4683961350601896No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA57590.3223581909430726No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA56140.3142418621209255No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT54920.30741295097401544No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA50840.28457528090893924No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT39790.22272325781602467No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC34540.19333655001169872No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT32720.1831491579728657No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT32330.18096614539311578No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG28940.16199072835375103No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28800.16120708281230234No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA28400.15896809555102037No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC27560.1542662223023282No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT27010.1511876148180655No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT26890.15051591863968092No Hit
GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC25700.14385493153736703No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA25350.1418958176837453No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC24640.13792161529496977No Hit
ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG24350.13629834953054035No Hit
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT24320.1361304254859442No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23490.1314845269187841No Hit
TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC23180.12974931179129057No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT23170.1296933371097585No Hit
CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT22900.12818202070839319No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC22570.12633485621783555No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC22450.12566316003945094No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA22240.12448769172727792No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC22080.12359209682276512No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT21910.12264052723672028No Hit
GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT21600.12090531210922675No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT21530.1205134893385024No Hit
CCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCAATTAATTCCATA21130.11827450207722043No Hit
GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA20430.11435627436997696No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC20000.11194936306409885No Hit
ATACTATGTAGTAGCCCATTTCCACTATGTTCTATCAATGGGAGCAGTGT19370.10842295812757974No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA19340.10825503408298358No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA18730.10484057850952858No Hit
CTATGTAGTAGCCCATTTCCACTATGTTCTATCAATGGGAGCAGTGTTTG18480.10344121147122733No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG17910.10025065462390052No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTCG150.00972793748.22379764-65
CATTCCG6100.042.7150349
AACGTCG250.001500530638.57903764-65
GACATAT8050.038.2701231
GTGCGCG500.001637135937.899889
GGCATTC7150.036.4421927
TAGGCAT8500.032.883725
CGGAGAT300.00367001132.149266-67
CATATAG9950.031.9006563
TTAGGCA9050.031.4087414
CCTTTAG8500.031.2256531
CTTTAGG8500.031.216912
CGCATAG2700.030.3631366-67
GTACATG90900.027.895381
GCGTATC700.00848293427.0834731
CGTATCA700.00849452627.0758912
GCGAGCT700.00850148727.0713469
AACGCAT3500.026.86754464-65
AGGCTTA13700.026.6267436
TCGGACT458.5555663E-426.59401170-71
Produced by FastQC (version 0.11.5)