FastQCFastQC Report
Fri 27 May 2016
SRR939448_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939448_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1771765
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92140.5200463944146092No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68030.3839673997398075No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT54770.3091267747133508No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52850.29829012312580955No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA40470.22841629674364264No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36870.20809757501700282No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT35730.2016633131369002No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA32030.18078018247340927No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT26180.14776225966761958No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC22580.12744353794097976No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT20660.11660688635343852No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC20140.11367195988181276No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA17780.10035190897212667No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTCCG6600.034.524089
GTACATG77150.031.4832121
GGCATTC7700.030.8513037
CATGGGG15400.030.3359414
GACATAT7150.029.5400411
TTAGGCA8300.028.7173294
CTTTAGG8250.028.4493872
TACATGG82000.028.2650722
ACATGGG80600.027.630613
CCTTTAG8700.027.1672971
TAGGCAT9200.025.8414275
GTATGGT4400.025.1442011
ACTCCCG4250.025.0965776
CATGGGA54000.024.8947334
CATATAG8950.024.1843263
TCTCGCG500.001688212223.6673994-95
GATTGGC12350.023.1607849
GAGTACT77300.022.91739512-13
CTCCCGC4950.022.526357
GCATTCC10950.022.137918
Produced by FastQC (version 0.11.5)