FastQCFastQC Report
Fri 27 May 2016
SRR939448_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939448_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1771765
Sequences flagged as poor quality0
Sequence length101
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119110.6722674846833525No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108510.6124401373771352No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT86530.4883830530572621No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83430.4708863760148778No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT54050.30506303036802285No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA53740.3033133626637844No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA53690.30303115819535886No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA48480.2736254525854162No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT39270.2216433895014294No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT32700.1845617223503117No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC32540.18365866805134992No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT31370.17705508349019197No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27820.1570185662319777No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG27620.15588974835827552No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC27410.15470448959088817No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT27110.15301126278033486No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT27020.15250329473716887No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA25900.14618191464443647No Hit
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT24770.139804093658019No Hit
ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG24180.13647408093059746No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA23980.13534526305689526No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC23360.13184592764841838No Hit
GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC22990.1297576145820693No Hit
TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC22840.12891100117679263No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC22720.12823371045257131No Hit
CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT22630.1277257424094053No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT22550.12727421525992444No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC22140.12496013861883488No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21950.12388776163881779No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA21820.12315403002091135No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT21550.12163012589141335No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC21320.12033198533665582No Hit
GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA20320.11468789596814477No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC20160.11378484166918298No Hit
CCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCAATTAATTCCATA20150.11372840077549788No Hit
GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT19630.11079347430387214No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT19590.11056771072913169No Hit
CTATGTAGTAGCCCATTTCCACTATGTTCTATCAATGGGAGCAGTGTTTG19420.10960821553648481No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA19340.10915668838700392No Hit
ATACTATGTAGTAGCCCATTTCCACTATGTTCTATCAATGGGAGCAGTGT19050.10751990247013571No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA18180.10260954471953108No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACATAT7750.039.197731
CATTCCG6950.034.7928289
CGCAAGT351.9321784E-434.2840664-65
CCGCAAT501.1984994E-633.59837764-65
TCGAGTC300.0037541332.00074466-67
TGTCGCG300.003755443531.99845564-65
GCACCGT600.003986834531.6091696
GGCATTC8050.030.0385257
CATATAG10500.029.8239733
CCTTTAG8300.029.7377381
CTTTAGG8750.029.285862
TGGGCGA650.00589704929.1776946
GACAATG6700.027.5990527
CCGATAG350.00797496327.42920968-69
TCGACGA350.00797496327.42920966-67
CGCGTAG350.00804749827.37835562-63
TTAGGCA9400.027.2630844
TAGGCAT9250.027.16685
GTATGGT3150.027.123431
GTACATG90750.026.5705011
Produced by FastQC (version 0.11.5)