FastQCFastQC Report
Fri 27 May 2016
SRR939447_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939447_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2828595
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56220.19875591945824694No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47310.16725618195605946No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91900.030.4452291
GTATCAA133300.030.2850271
TATCAAC157400.025.4658582
GTACATG94250.025.180191
ATCAACG158050.025.1262113
TACATGG93550.024.6030752
TCAACGC160850.024.5515754
CAACGCA160950.024.3596385
AACGCAG162300.024.0570476
GAGTACT80150.022.7025712-13
ACATGGG99250.022.2503763
GTACTTT83950.021.63796814-15
ACGCAGA178600.021.4658577
CGCAGAG178550.021.4406038
AGAGTAC126500.020.34148610-11
GCAGAGT182550.019.5729189
AGTACTT86050.018.86443312-13
ACTTTTT109050.017.94740916-17
CAGAGTA174650.017.42757210-11
CATGGGG54150.017.237834
Produced by FastQC (version 0.11.5)