FastQCFastQC Report
Fri 27 May 2016
SRR939447_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939447_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2828595
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81190.28703296159400693No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74100.2619675139070811No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45790.16188248936309368No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT34470.12186262084179601No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA97750.029.2849861
GTATCAA136600.026.8543781
GTACATG111750.022.3081251
ATCAACG164500.022.0334343
TCAACGC165300.021.8981344
TATCAAC168450.021.6878262
TACATGG115000.021.4258582
CAACGCA168800.021.387955
AACGCAG172200.021.0206836
ACATGGG112700.020.641643
GAGTACT89400.019.45014612-13
AGAGTAC148650.019.10781110-11
ACGCAGA193300.018.6771147
CGCAGAG195550.018.389538
GTACTTT92900.018.30935714-15
AGTACTT95300.018.19627612-13
CATGGGA60050.016.9633674
GCAGAGT206350.016.7152779
TCTATAC7400.016.0064453
TACTTTT108400.015.909862514-15
Produced by FastQC (version 0.11.5)