##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939447_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2828595 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.01282615574163 31.0 31.0 33.0 30.0 34.0 2 31.286667762617128 31.0 31.0 34.0 30.0 34.0 3 31.28813244738112 31.0 31.0 34.0 28.0 34.0 4 35.21231459434808 35.0 35.0 37.0 33.0 37.0 5 34.83072231973824 35.0 35.0 37.0 32.0 37.0 6 34.78409033460075 35.0 35.0 37.0 32.0 37.0 7 34.654303284846364 35.0 35.0 37.0 30.0 37.0 8 34.668398975463084 35.0 35.0 37.0 30.0 37.0 9 36.04199646821125 37.0 35.0 39.0 30.0 39.0 10-11 35.89483648242325 37.0 35.0 39.0 30.0 39.0 12-13 35.86503352371054 37.0 35.0 39.0 30.0 39.0 14-15 36.75716672058036 38.0 36.0 40.0 31.0 41.0 16-17 36.759734956754144 38.0 36.0 40.0 31.0 41.0 18-19 36.69364967413151 38.0 35.0 40.0 30.0 41.0 20-21 36.57926408694069 38.0 35.0 40.0 30.0 41.0 22-23 36.440347946595395 38.0 35.0 40.0 30.0 41.0 24-25 36.23473261460195 38.0 34.0 40.0 29.5 41.0 26-27 35.87399786819958 38.0 34.0 40.0 28.0 41.0 28-29 35.6138379654917 38.0 34.0 40.0 27.0 41.0 30-31 35.42001753520741 38.0 34.0 40.0 27.0 41.0 32-33 35.244125440368805 38.0 34.0 40.0 27.0 41.0 34-35 35.25811683892533 38.0 34.0 40.0 27.0 41.0 36-37 35.30253217586824 38.0 34.0 40.0 27.0 41.0 38-39 35.287341772151905 38.0 34.0 40.0 27.0 41.0 40-41 35.10029785105326 38.0 33.5 40.0 26.5 41.0 42-43 34.831504863722095 38.0 33.0 40.0 25.5 41.0 44-45 34.6859624654643 38.0 33.0 40.0 25.0 41.0 46-47 34.428748371541346 38.0 33.0 40.0 24.5 41.0 48-49 34.15642942874466 37.0 33.0 40.0 24.0 41.0 50-51 33.91768757987623 37.0 32.5 40.0 23.5 41.0 52-53 33.56435385765725 37.0 32.0 40.0 23.0 41.0 54-55 33.35389389431856 37.0 31.5 39.5 22.5 41.0 56-57 32.95424565906396 36.0 31.0 39.0 21.0 40.5 58-59 32.52031026004076 36.0 31.0 39.0 19.5 40.0 60-61 32.23716862965536 35.5 30.5 39.0 19.0 40.0 62-63 31.851278638334577 35.0 30.0 39.0 18.0 40.0 64-65 31.420509122019944 35.0 29.5 38.0 14.5 40.0 66-67 29.66814814422001 33.5 26.5 37.5 10.0 39.5 68-69 25.88089563900099 30.5 17.0 35.5 6.5 39.0 70-71 25.26148900779362 29.5 16.0 35.0 6.0 38.0 72-73 27.165457409067045 31.0 22.5 35.0 6.5 38.0 74-75 27.810162289051632 32.0 25.0 35.0 6.5 38.0 76-77 27.885579589867053 31.5 26.0 34.5 6.0 36.5 78-79 28.74691162927178 33.0 27.5 35.0 2.0 37.0 80-81 28.602585382495548 33.0 27.5 35.0 2.0 37.0 82-83 28.19438060238387 33.0 26.5 35.0 2.0 36.0 84-85 27.85423134100145 33.0 26.0 35.0 2.0 36.0 86-87 27.419810895515262 32.5 26.0 35.0 2.0 35.5 88-89 26.93847829045869 32.0 25.0 34.5 2.0 35.0 90-91 26.49733843127065 32.0 25.0 34.0 2.0 35.0 92-93 25.938625890238793 32.0 23.5 34.0 2.0 35.0 94-95 24.525877688393003 31.0 18.0 34.0 2.0 35.0 96-97 23.727382499085234 31.0 8.5 34.0 2.0 35.0 98-99 23.422994808376597 31.0 2.0 34.0 2.0 35.0 100-101 22.153160491339342 30.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3922905506795331 1101 2 0.25700785518190017 1101 3 0.32096421779008466 1101 4 0.26265778649317184 1101 5 0.36448522919445026 1101 6 0.53902888289781 1101 7 0.4189475356032304 1101 8 0.47961129282106896 1101 9 0.6517994537753822 1101 10-11 0.6756170605442975 1101 12-13 0.598255693300132 1101 14-15 0.7125813507687084 1101 16-17 0.6850574305904544 1101 18-19 0.7506708502174106 1101 20-21 0.7157178922587093 1101 22-23 0.792564863221024 1101 24-25 0.7356885141230549 1101 26-27 0.703468647120232 1101 28-29 0.6238624566224473 1101 30-31 0.7182598124823869 1101 32-33 0.6223626406273652 1101 34-35 0.7146475314851557 1101 36-37 0.700028689515868 1101 38-39 0.731249988915259 1101 40-41 0.911639684757084 1101 42-43 0.8900785431956919 1101 44-45 0.5496430350459747 1101 46-47 0.6513421009539897 1101 48-49 0.6465932349539827 1101 50-51 0.6345623309061992 1101 52-53 0.6563803935470389 1101 54-55 0.6177469945343077 1101 56-57 0.5821710934484585 1101 58-59 0.4381994585394082 1101 60-61 0.33022916245216294 1101 62-63 0.2785194644991087 1101 64-65 0.017216104864477444 1101 66-67 -2.9721142889832173 1101 68-69 -8.101144444291677 1101 70-71 -11.586421154336755 1101 72-73 -11.699627970230733 1101 74-75 -9.865119766838625 1101 76-77 -4.678816307539396 1101 78-79 -1.5728043938755363 1101 80-81 -1.0349697814371623 1101 82-83 -0.7014386181744037 1101 84-85 -0.9118964381574592 1101 86-87 -0.8147974153040778 1101 88-89 -0.6413092140676078 1101 90-91 -0.7840261608898054 1101 92-93 -1.4576076913639753 1101 94-95 -5.516771463961064 1101 96-97 -2.1287038178055653 1101 98-99 -1.166036029100237 1101 100-101 -0.8210254091144478 1102 1 0.3541838268531663 1102 2 0.34451898542854664 1102 3 0.42558197772860495 1102 4 0.25623387880052917 1102 5 0.4336729256616323 1102 6 0.5249962845851286 1102 7 0.5728454752923398 1102 8 0.5402551264715285 1102 9 0.6057229332332241 1102 10-11 0.7500832330820302 1102 12-13 0.6996032440285944 1102 14-15 0.6499105390978812 1102 16-17 0.6404878335632489 1102 18-19 0.7516430589367289 1102 20-21 0.8243793846145238 1102 22-23 0.9081876786448788 1102 24-25 0.8883833455220014 1102 26-27 0.8527860177123117 1102 28-29 0.8280272521731291 1102 30-31 0.5811212850853948 1102 32-33 0.6491300157718385 1102 34-35 0.7709931995237156 1102 36-37 0.9744862973577355 1102 38-39 0.8172962475320418 1102 40-41 0.8424782224934475 1102 42-43 0.9491456240541467 1102 44-45 0.890958703501191 1102 46-47 0.7748843665706744 1102 48-49 0.6887137919215576 1102 50-51 0.6681598581010348 1102 52-53 0.759026923147708 1102 54-55 0.6852979467587446 1102 56-57 0.5327248280416512 1102 58-59 0.47103696542588835 1102 60-61 0.46364146845407816 1102 62-63 0.17973244919530984 1102 64-65 0.2631089088224492 1102 66-67 -3.0341167071595088 1102 68-69 -8.25475438643754 1102 70-71 -11.466397551269512 1102 72-73 -9.726366603490458 1102 74-75 -8.024848451162452 1102 76-77 -4.374144613164784 1102 78-79 -1.5067738680649363 1102 80-81 -0.7054849671830397 1102 82-83 -0.5069401621344412 1102 84-85 -0.48671870142849727 1102 86-87 -0.5071618346254141 1102 88-89 -0.43663690241761444 1102 90-91 -0.44327009114385874 1102 92-93 -0.8933614771910214 1102 94-95 -4.638088644740961 1102 96-97 -1.3765962081890315 1102 98-99 -0.6636516100566752 1102 100-101 -0.5897307820336479 1103 1 0.3582021326617131 1103 2 0.2633712905207197 1103 3 0.3849824833639701 1103 4 0.22745282865081862 1103 5 0.41639820986417675 1103 6 0.33731306131616634 1103 7 0.3421954173911743 1103 8 0.4051811068790272 1103 9 0.6778542175957369 1103 10-11 0.5462255030162027 1103 12-13 0.5276611382346488 1103 14-15 0.6859353200993397 1103 16-17 0.5952245136880236 1103 18-19 0.5413662114028384 1103 20-21 0.7209432202100032 1103 22-23 0.6024126029212837 1103 24-25 0.7971794534511574 1103 26-27 0.7402225959449495 1103 28-29 0.8346545877807756 1103 30-31 0.8444111664662515 1103 32-33 0.7861549393527483 1103 34-35 0.7722598960972817 1103 36-37 0.7791915926188864 1103 38-39 0.7241262372037269 1103 40-41 0.7640947429292453 1103 42-43 0.8036333117982224 1103 44-45 0.6257744282741982 1103 46-47 0.7654432605715584 1103 48-49 0.6310482345924484 1103 50-51 0.6448168224109665 1103 52-53 0.6643393981528405 1103 54-55 0.6894127545025484 1103 56-57 0.683497799849178 1103 58-59 0.684698770481539 1103 60-61 0.6368463585809749 1103 62-63 0.5441776587001392 1103 64-65 0.2910883843387744 1103 66-67 -2.9000272681226456 1103 68-69 -7.748354395479161 1103 70-71 -11.871982144344326 1103 72-73 -9.353516597056991 1103 74-75 -7.527824362192884 1103 76-77 -3.8898496510874345 1103 78-79 -1.0795137231903205 1103 80-81 -0.4242083422121645 1103 82-83 -0.09004832428551168 1103 84-85 -0.21378297345614783 1103 86-87 -0.07404550023838397 1103 88-89 0.017434696802023808 1103 90-91 -0.15075751111680447 1103 92-93 -0.028611982304756367 1103 94-95 0.7988525568907043 1103 96-97 0.9426630568661416 1103 98-99 0.6233838331045547 1103 100-101 0.7940834960797112 1104 1 0.34783890021189734 1104 2 0.2055750014489739 1104 3 0.34935763281497145 1104 4 0.16147328801280736 1104 5 0.3182251804856051 1104 6 0.4830119124606469 1104 7 0.5510115586779918 1104 8 0.48611243477792243 1104 9 0.6471448763069887 1104 10-11 0.5603385624798847 1104 12-13 0.6160894168825592 1104 14-15 0.7408387689423535 1104 16-17 0.7952476954888326 1104 18-19 0.7206744528080904 1104 20-21 0.8989025729521174 1104 22-23 0.8028813040281051 1104 24-25 0.8607093492952558 1104 26-27 0.9439955099077437 1104 28-29 1.1026934007928944 1104 30-31 1.0824911537333364 1104 32-33 0.8725772429009666 1104 34-35 0.9235393579228628 1104 36-37 0.9658617156882556 1104 38-39 0.8841126752811377 1104 40-41 0.8808016884490826 1104 42-43 1.1010636888543885 1104 44-45 0.9031230012702594 1104 46-47 0.7988540309841738 1104 48-49 0.8740893548766948 1104 50-51 0.8172119738481669 1104 52-53 1.0608658557109365 1104 54-55 0.9905998353342795 1104 56-57 0.9267351212584884 1104 58-59 0.9417936678010577 1104 60-61 0.7320762762315169 1104 62-63 0.985799643014861 1104 64-65 0.7026408033321836 1104 66-67 -2.4811339297318113 1104 68-69 -7.306161023406425 1104 70-71 -9.015536441784473 1104 72-73 -8.381835623406186 1104 74-75 -7.105214110392431 1104 76-77 -3.4730292532274056 1104 78-79 -0.4564205899261715 1104 80-81 0.24121804120021295 1104 82-83 0.42060520235975574 1104 84-85 0.5264532817231213 1104 86-87 0.7050325071852939 1104 88-89 0.6586084964727554 1104 90-91 0.5798440689448725 1104 92-93 0.700296159619846 1104 94-95 1.546967873639563 1104 96-97 1.6386371539544449 1104 98-99 0.9965370313251718 1104 100-101 0.9354406918709799 1105 1 0.46301234344623055 1105 2 0.3065231034153939 1105 3 0.3991271731000623 1105 4 0.3407085093928828 1105 5 0.4434414985276405 1105 6 0.5663997541287955 1105 7 0.5368130904003152 1105 8 0.5281387421012482 1105 9 0.8006588342524736 1105 10-11 0.83847262973174 1105 12-13 0.82342235074222 1105 14-15 0.8624963693101151 1105 16-17 0.900075895128083 1105 18-19 0.9852625317971544 1105 20-21 1.0443970397843714 1105 22-23 1.0153485037329162 1105 24-25 1.0863442665721834 1105 26-27 0.9900072150961776 1105 28-29 1.1637497399216414 1105 30-31 1.0327436740767624 1105 32-33 1.130556567470279 1105 34-35 1.279505180815761 1105 36-37 1.0920067242494937 1105 38-39 1.0759118772486218 1105 40-41 1.0788129347002027 1105 42-43 1.1258626941344474 1105 44-45 0.9529148404195951 1105 46-47 0.9828543481235812 1105 48-49 0.8736132645956403 1105 50-51 0.8883027065926612 1105 52-53 1.188520373498946 1105 54-55 1.159633898088238 1105 56-57 1.1889952083498514 1105 58-59 1.2237271661078708 1105 60-61 1.3315415513728155 1105 62-63 1.3817117847273828 1105 64-65 1.2420374170304704 1105 66-67 1.9304518358930203 1105 68-69 -5.125721996887165 1105 70-71 -5.364123341792556 1105 72-73 -1.605677912115869 1105 74-75 1.0668472137713039 1105 76-77 1.3577524325057446 1105 78-79 1.1735540103333335 1105 80-81 1.1475491366424038 1105 82-83 1.2702989932124176 1105 84-85 1.1613058061832575 1105 86-87 1.2867192981004685 1105 88-89 1.3867089153102263 1105 90-91 1.2669265846531843 1105 92-93 1.274774318549376 1105 94-95 2.1250584009295643 1105 96-97 2.314811570334083 1105 98-99 1.857117670879468 1105 100-101 1.5826336365103373 1106 1 0.16416566773388297 1106 2 0.12753169681204568 1106 3 0.2580428221276492 1106 4 0.13628083714804973 1106 5 0.26598126183232296 1106 6 0.3345099606718378 1106 7 0.3981738999887412 1106 8 0.31295047887194016 1106 9 0.3832666286140025 1106 10-11 0.4412824442952825 1106 12-13 0.3144685402492229 1106 14-15 0.3681781874919281 1106 16-17 0.33177393899963903 1106 18-19 0.31478678552849715 1106 20-21 0.42405415195081275 1106 22-23 0.46488349921008165 1106 24-25 0.5703824091924119 1106 26-27 0.7675871186089225 1106 28-29 0.6701830091814074 1106 30-31 0.6574303731571192 1106 32-33 0.625675668932665 1106 34-35 0.7191865090232028 1106 36-37 0.5016841891974835 1106 38-39 0.5958241529911277 1106 40-41 0.6429336191567074 1106 42-43 0.6224517187085539 1106 44-45 0.5900581956133237 1106 46-47 0.4395010389120628 1106 48-49 0.37561085241411973 1106 50-51 0.24188517304826007 1106 52-53 0.5469837384921661 1106 54-55 0.5341321454876038 1106 56-57 0.4153963465980013 1106 58-59 0.6582354956721659 1106 60-61 0.3784141957268048 1106 62-63 0.5036918465395352 1106 64-65 0.6405703206939712 1106 66-67 1.307689699604765 1106 68-69 -5.60399822936116 1106 70-71 -6.664390472050357 1106 72-73 -2.385427365605995 1106 74-75 0.5491162662362541 1106 76-77 0.5993898891618556 1106 78-79 0.5406366840857828 1106 80-81 0.49225081763139755 1106 82-83 0.48302089506678314 1106 84-85 0.5534831769054058 1106 86-87 0.5744779480326265 1106 88-89 0.526229117330427 1106 90-91 0.5576839719590794 1106 92-93 0.5245128418420038 1106 94-95 1.5919168171816374 1106 96-97 1.7175634360057224 1106 98-99 1.3752113879004249 1106 100-101 1.3192111655318932 1107 1 -0.029421387960795187 1107 2 -0.04364111393475767 1107 3 -0.030470514311694785 1107 4 -0.031908705621091826 1107 5 0.15873992631184564 1107 6 0.08567875948637749 1107 7 0.11379604876671578 1107 8 0.10765946009998117 1107 9 0.08439749202064917 1107 10-11 0.1267823423792649 1107 12-13 0.15816121248730042 1107 14-15 0.19159230140493122 1107 16-17 0.10190447643679335 1107 18-19 0.15685183464231045 1107 20-21 0.2031084076726657 1107 22-23 0.1772834637821319 1107 24-25 0.2700385235065994 1107 26-27 0.3879163720659733 1107 28-29 0.26831529698578294 1107 30-31 0.13134967912519357 1107 32-33 0.23115652651554086 1107 34-35 0.24476237498583941 1107 36-37 0.2371772322157497 1107 38-39 0.03384960692185501 1107 40-41 0.09858275950315942 1107 42-43 0.35899475962379057 1107 44-45 0.0819800365402088 1107 46-47 0.008890885488007427 1107 48-49 0.08049764474485954 1107 50-51 0.16711056113799572 1107 52-53 0.2979288882601807 1107 54-55 0.1474994629608588 1107 56-57 0.30687135452480163 1107 58-59 0.2869552282315837 1107 60-61 0.15829055379823131 1107 62-63 0.10423665238142732 1107 64-65 0.18969990934149905 1107 66-67 0.9451887694188592 1107 68-69 -5.689288592958697 1107 70-71 -6.339580775954225 1107 72-73 -2.3138120492457226 1107 74-75 0.21895233916929868 1107 76-77 0.21351197161353497 1107 78-79 0.14879880254778755 1107 80-81 0.04095616061925256 1107 82-83 0.18112592437977426 1107 84-85 0.12993091016367586 1107 86-87 0.2665681121659489 1107 88-89 0.2813544416822076 1107 90-91 0.2697774807173836 1107 92-93 0.17997754250009024 1107 94-95 1.1063584589354996 1107 96-97 1.017955145380057 1107 98-99 0.5440649014148136 1107 100-101 0.5085084464771583 1108 1 -0.02734685381721036 1108 2 -0.042572186768211395 1108 3 -0.02198807995002028 1108 4 -0.08921635999210764 1108 5 -0.06262148453199501 1108 6 0.08785707696495137 1108 7 -0.00620590334839477 1108 8 -0.07104710302221662 1108 9 0.12996517141910147 1108 10-11 0.06484783542938999 1108 12-13 0.05493355076908557 1108 14-15 -0.021694807160471896 1108 16-17 -0.08937325497482362 1108 18-19 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-0.003208031523055155 2316 84-85 0.3927695263149964 2316 86-87 -0.06479995422943219 2316 88-89 0.5821314505214907 2316 90-91 0.6315164872528634 2316 92-93 0.9026538469636449 2316 94-95 1.152925228762232 2316 96-97 1.075618013470514 2316 98-99 0.28074338036023505 2316 100-101 0.22834653329402244 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 23.0 4 49.0 5 148.0 6 353.0 7 943.0 8 2229.0 9 3689.0 10 5725.0 11 8407.0 12 11575.0 13 14996.0 14 17915.0 15 22560.0 16 27003.0 17 30794.0 18 30152.0 19 27298.0 20 27141.0 21 27622.0 22 29257.0 23 33470.0 24 41127.0 25 51367.0 26 65430.0 27 81707.0 28 100831.0 29 120545.0 30 143179.0 31 166237.0 32 194080.0 33 225426.0 34 263484.0 35 305694.0 36 330586.0 37 284550.0 38 124740.0 39 8262.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.17773943817114 20.099167486756276 15.565695631064699 23.157397444007888 2 15.972662502912776 20.89190564405173 39.205963670463504 23.92946818257199 3 19.555150171728368 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26.951610768513202 28.001050324262845 22.376294945774532 48-49 22.636589630284465 26.98524736972208 27.92777505180576 22.450387948187696 50-51 22.508155276175266 27.133341685015594 27.987000394227714 22.37150264458142 52-53 22.745643174226682 27.21998480767414 27.552117921319034 22.482254096780142 54-55 22.557849779305712 26.994747698868064 27.73667458975131 22.71072793207491 56-57 22.649940292843578 27.20878017916921 27.628489747574804 22.512789780412408 58-59 22.777271753297036 26.986955411778602 27.67498566198492 22.560787172939445 60-61 22.68776996833126 27.0349119855067 27.9345559817575 22.34276206440454 62-63 22.55988395484053 27.02131548487319 27.943783289977848 22.475017270308435 64-65 22.456513745898857 27.06801886525625 27.764930280574724 22.710537108270167 66-67 21.912603385454208 27.129419024698887 28.195353504222766 22.76262408562414 68-69 21.791807517184566 26.77863770412854 28.846096947932807 22.58345783075409 70-71 21.48119947707344 26.66764905690537 29.1199377169346 22.73121374908659 72-73 22.36780012501405 26.907855853057793 27.92330249818914 22.80104152373902 74-75 22.079478879493184 27.26087042732476 27.784678399927486 22.874972293254565 76-77 22.376849565845383 27.077069405513583 27.499741443092283 23.046339585548747 78-79 22.331885669756986 27.10397809606695 27.557882235599866 23.006253998576202 80-81 22.468661538249414 27.09615128300583 27.435211572843077 22.999975605901675 82-83 22.42364141914979 27.117774018549845 27.49958901857636 22.958995543724004 84-85 22.32184176242976 27.28766401694127 27.253742582448176 23.136751638180794 86-87 22.21187744556648 27.1988179261172 27.500470199265497 23.08883442905082 88-89 22.26019969401581 27.08921710647843 27.235580428678375 23.415002770827385 90-91 22.211668916094148 27.104673064056094 27.36413944152328 23.319518578326477 92-93 22.352177117179128 27.005027849118918 27.41764274253322 23.22515229116873 94-95 21.74942737109471 26.912087997754842 27.77512409321945 23.563360537930993 96-97 21.51204810844706 26.862587262473937 27.97149554312213 23.65386908595687 98-99 21.932672903243486 26.812813416271613 27.294054626472775 23.960459054012127 100-101 21.801020757211568 26.631594894693645 27.526874383151835 24.04050996494296 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 726.0 1 500.0 2 306.5 3 349.0 4 364.5 5 415.0 6 585.5 7 1055.0 8 1648.5 9 2069.0 10 2194.5 11 2158.5 12 2199.0 13 2330.5 14 2506.0 15 2663.5 16 2865.0 17 3135.5 18 3418.0 19 3753.0 20 4245.0 21 4919.0 22 5828.5 23 7088.0 24 8598.0 25 10698.5 26 13860.5 27 17401.5 28 21118.5 29 25568.5 30 30374.0 31 35976.0 32 42546.0 33 48802.0 34 55482.0 35 61952.0 36 68507.0 37 74568.0 38 78488.5 39 81287.0 40 84652.5 41 88613.0 42 92120.0 43 96382.5 44 100135.5 45 102981.5 46 105739.5 47 109886.5 48 115071.0 49 114731.0 50 109726.5 51 105501.0 52 102792.5 53 100800.0 54 97309.0 55 91046.0 56 83328.5 57 75067.5 58 67002.0 59 58265.5 60 48506.0 61 38680.0 62 29708.0 63 22500.0 64 16319.0 65 11458.5 66 8037.0 67 5864.0 68 4280.0 69 2964.0 70 2060.5 71 1502.5 72 1088.0 73 786.0 74 553.0 75 352.0 76 226.0 77 141.0 78 91.5 79 52.0 80 29.5 81 18.5 82 13.5 83 9.0 84 7.5 85 4.0 86 1.0 87 1.0 88 1.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02305031296456368 2 0.019020043519839356 3 0.0 4 0.0 5 7.070648148639166E-5 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 5.302986111479374E-4 18-19 0.001219686805640256 20-21 1.7676620371597915E-5 22-23 0.0 24-25 0.0 26-27 5.479752315195353E-4 28-29 0.017499854167881933 30-31 0.0046312745373586536 32-33 0.00813124537093504 34-35 0.00973981782475045 36-37 0.10004967130324419 38-39 0.10312540324790222 40-41 0.11896365510085395 42-43 0.10234763195155193 44-45 0.09762797431233528 46-47 0.09902442732169152 48-49 0.10236530857192351 50-51 0.09959007917358265 52-53 0.07664582593124855 54-55 0.06483784352302115 56-57 0.036926459956268046 58-59 0.015873605093694926 60-61 0.00897972314877174 62-63 0.001449482870471029 64-65 0.00406562268546752 66-67 0.43106206438178674 68-69 0.8242961611683539 70-71 0.9158964079339743 72-73 0.2867854889088045 74-75 0.15459972176999534 76-77 0.014052913195420342 78-79 0.008643867361711379 80-81 0.0014848361112142247 82-83 0.0 84-85 0.0 86-87 2.474726852023708E-4 88-89 6.186817130059269E-4 90-91 0.0034469409724615934 92-93 0.0017499854167881933 94-95 0.23060918936786637 96-97 0.23759145441464755 98-99 0.013699380787988383 100-101 0.007229737731983547 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2828595.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.36186746298654 #Duplication Level Percentage of deduplicated Percentage of total 1 75.49322766860743 43.30432519879727 2 14.083537458452591 16.157160182035273 3 4.659780747289606 8.018811768978082 4 2.0134998982089707 4.619924571911994 5 1.0896043262722837 3.125086947536373 6 0.6263316432759354 2.1556531625681257 7 0.4027024689833008 1.6169835956986283 8 0.2754573638072818 1.2640599035533568 9 0.206732574476455 1.0672709883660365 >10 1.041052379078562 10.87270112229472 >50 0.07485376132309694 2.9186693294356867 >100 0.031357472603420235 3.221449065299134 >500 0.0012414917474264078 0.48824513844685097 >1k 4.965966989319391E-4 0.6167157086778496 >5k 1.2414917473298477E-4 0.5529433164005961 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8119 0.28703296159400693 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7410 0.2619675139070811 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4579 0.16188248936309368 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3447 0.12186262084179601 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 3.5353240743195824E-5 0.0 4 0.0 0.0 0.0 3.5353240743195824E-5 0.0 5 0.0 0.0 0.0 3.5353240743195824E-5 3.5353240743195824E-5 6 0.0 0.0 0.0 3.5353240743195824E-5 3.5353240743195824E-5 7 0.0 0.0 0.0 3.5353240743195824E-5 3.5353240743195824E-5 8 0.0 0.0 0.0 3.5353240743195824E-5 3.5353240743195824E-5 9 0.0 0.0 0.0 3.5353240743195824E-5 3.5353240743195824E-5 10-11 0.0 0.0 0.0 3.5353240743195824E-5 3.5353240743195824E-5 12-13 0.0 0.0 0.0 5.302986111479374E-5 1.414129629727833E-4 14-15 0.0 0.0 0.0 8.838310185798956E-5 1.5908958334438122E-4 16-17 0.0 0.0 0.0 1.2373634260118537E-4 2.1211944445917496E-4 18-19 0.0 0.0 0.0 1.414129629727833E-4 2.2979606483077288E-4 20-21 0.0 0.0 0.0 1.767662037159791E-4 2.828259259455666E-4 22-23 0.0 0.0 0.0 2.1211944445917496E-4 3.1817916668876243E-4 24-25 1.7676620371597912E-5 0.0 0.0 3.1817916668876243E-4 3.1817916668876243E-4 26-27 3.5353240743195824E-5 0.0 0.0 4.7726875003314365E-4 3.535324074319583E-4 28-29 3.5353240743195824E-5 0.0 0.0 0.0012727166667550497 3.535324074319583E-4 30-31 3.5353240743195824E-5 0.0 0.0 0.004560568055872262 3.535324074319583E-4 32-33 3.5353240743195824E-5 0.0 0.0 0.012108484954544571 3.535324074319583E-4 34-35 3.5353240743195824E-5 0.0 0.0 0.02548968657584419 3.535324074319583E-4 36-37 3.5353240743195824E-5 0.0 0.0 0.048893531947839824 3.535324074319583E-4 38-39 3.5353240743195824E-5 0.0 0.0 0.08115336412600602 3.535324074319583E-4 40-41 3.5353240743195824E-5 0.0 0.0 0.11565812709136514 3.535324074319583E-4 42-43 3.5353240743195824E-5 0.0 0.0 0.1516300495475669 3.535324074319583E-4 44-45 3.5353240743195824E-5 0.0 0.0 0.19857915325453096 3.535324074319583E-4 46-47 3.5353240743195824E-5 0.0 0.0 0.2549675722399283 3.535324074319583E-4 48-49 3.5353240743195824E-5 0.0 0.0 0.3149796984015032 3.535324074319583E-4 50-51 3.5353240743195824E-5 0.0 0.0 0.3786862382207421 3.535324074319583E-4 52-53 3.5353240743195824E-5 0.0 0.0 0.440589762762078 3.888856481751541E-4 54-55 3.5353240743195824E-5 0.0 0.0 0.5106245326743489 4.242388889183499E-4 56-57 3.5353240743195824E-5 1.7676620371597912E-5 0.0 0.5931036433282247 4.242388889183499E-4 58-59 5.302986111479374E-5 3.5353240743195824E-5 0.0 0.6763958785191941 4.242388889183499E-4 60-61 7.070648148639165E-5 3.5353240743195824E-5 0.0 0.7585744866267529 4.242388889183499E-4 62-63 7.070648148639165E-5 3.5353240743195824E-5 0.0 0.8423086373270121 4.5959212966154576E-4 64-65 1.0605972222958748E-4 3.5353240743195824E-5 0.0 0.9369315861761758 4.5959212966154576E-4 66-67 1.0605972222958748E-4 3.5353240743195824E-5 0.0 1.0326504854883785 4.5959212966154576E-4 68-69 1.414129629727833E-4 3.5353240743195824E-5 0.0 1.1472303387370761 4.5959212966154576E-4 70-71 1.414129629727833E-4 3.5353240743195824E-5 0.0 1.3015118813403828 5.126219907763394E-4 72-73 1.414129629727833E-4 3.5353240743195824E-5 0.0 1.4670357545000257 6.01005092634329E-4 74-75 1.414129629727833E-4 3.5353240743195824E-5 0.0 1.6482918197903906 6.363583333775249E-4 76-77 1.414129629727833E-4 3.5353240743195824E-5 0.0 1.8506007399433289 6.363583333775249E-4 78-79 1.414129629727833E-4 3.5353240743195824E-5 0.0 2.0897830901914203 6.363583333775249E-4 80-81 1.414129629727833E-4 3.5353240743195824E-5 0.0 2.3486925487742147 6.363583333775249E-4 82-83 1.414129629727833E-4 3.5353240743195824E-5 0.0 2.618968074255947 6.717115741207207E-4 84-85 1.414129629727833E-4 3.5353240743195824E-5 0.0 2.8890314802932195 7.247414352355144E-4 86-87 1.414129629727833E-4 3.5353240743195824E-5 0.0 3.170248833784971 8.13124537093504E-4 88-89 1.414129629727833E-4 3.5353240743195824E-5 0.0 3.4787588891304697 8.484777778366998E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9775 0.0 29.284986 1 GTATCAA 13660 0.0 26.854378 1 GTACATG 11175 0.0 22.308125 1 ATCAACG 16450 0.0 22.033434 3 TCAACGC 16530 0.0 21.898134 4 TATCAAC 16845 0.0 21.687826 2 TACATGG 11500 0.0 21.425858 2 CAACGCA 16880 0.0 21.38795 5 AACGCAG 17220 0.0 21.020683 6 ACATGGG 11270 0.0 20.64164 3 GAGTACT 8940 0.0 19.450146 12-13 AGAGTAC 14865 0.0 19.107811 10-11 ACGCAGA 19330 0.0 18.677114 7 CGCAGAG 19555 0.0 18.38953 8 GTACTTT 9290 0.0 18.309357 14-15 AGTACTT 9530 0.0 18.196276 12-13 CATGGGA 6005 0.0 16.963367 4 GCAGAGT 20635 0.0 16.715277 9 TCTATAC 740 0.0 16.006445 3 TACTTTT 10840 0.0 15.9098625 14-15 >>END_MODULE