FastQCFastQC Report
Fri 27 May 2016
SRR939446_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939446_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2811263
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54920.19535703347570116No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45170.16067511292966896No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28220.10038192798041308No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA132150.030.2670271
GGTATCA91750.027.328661
TATCAAC155550.025.0137772
ATCAACG157500.024.9491043
TCAACGC158900.024.6885014
CAACGCA160900.024.4206855
AACGCAG163100.024.1601946
GTACATG96000.023.0306021
GAGTACT71700.021.85210212-13
TACATGG99000.021.7246952
ACGCAGA178350.021.659537
CGCAGAG178700.021.0754178
ACATGGG101900.020.7678683
AGAGTAC121100.020.51301210-11
GTACTTT80000.019.86834314-15
GCAGAGT176300.019.3278149
GTGTAGC15900.018.3296741
AGTACTT79450.018.29895212-13
CATGGGA53550.017.5357994
ATCGACG1650.001347051217.4896343
Produced by FastQC (version 0.11.5)