##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939446_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2811263 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.94096994838263 31.0 26.0 31.0 10.0 33.0 2 27.021452279633746 31.0 26.0 31.0 16.0 34.0 3 27.309972777360212 31.0 26.0 31.0 16.0 34.0 4 31.115583991963753 35.0 30.0 37.0 19.0 37.0 5 31.51448868355611 35.0 31.0 37.0 19.0 37.0 6 31.32252869973389 35.0 30.0 37.0 17.0 37.0 7 31.289216981833434 35.0 30.0 37.0 17.0 37.0 8 31.212416269840283 35.0 30.0 37.0 17.0 37.0 9 32.28904837434278 35.0 30.0 39.0 17.0 39.0 10-11 31.396229915166245 35.0 29.5 38.5 14.0 39.0 12-13 31.14856560912302 35.0 28.5 38.0 13.0 39.0 14-15 31.982309374825476 36.0 29.0 39.0 16.5 40.0 16-17 31.88158525189568 36.0 29.0 39.0 16.5 40.0 18-19 31.778346245086283 36.0 28.0 39.0 15.5 40.0 20-21 31.627607057753046 36.0 27.0 39.0 13.0 40.0 22-23 31.8356898660851 36.0 29.0 39.0 13.5 40.0 24-25 31.926054054707794 36.0 29.5 39.0 13.5 41.0 26-27 32.04039198751593 36.0 30.0 39.0 15.5 41.0 28-29 31.649100955691445 36.0 29.0 39.0 10.0 40.5 30-31 31.375889946974013 36.0 28.5 39.0 10.0 40.0 32-33 31.26799555929132 35.5 29.0 39.0 9.5 40.0 34-35 30.84515625183414 35.0 27.0 39.0 9.0 40.0 36-37 30.511613641270845 35.0 26.5 38.5 9.0 40.0 38-39 30.1458015489835 35.0 26.0 38.0 9.0 40.0 40-41 30.014246977248305 35.0 25.5 38.0 8.5 40.0 42-43 29.782549516000458 35.0 25.0 38.0 8.0 40.0 44-45 29.456387929553372 34.0 25.0 38.0 4.5 40.0 46-47 28.924880738657322 34.0 24.0 38.0 2.0 40.0 48-49 28.629330126708176 34.0 24.0 38.0 2.0 40.0 50-51 27.990961891505705 33.0 23.0 37.5 2.0 39.5 52-53 28.10300886114177 33.0 23.0 38.0 2.0 39.5 54-55 28.23057714628621 34.0 23.0 38.0 2.0 40.0 56-57 27.785947810645965 33.5 22.5 38.0 2.0 40.0 58-59 27.394425566017837 33.0 22.0 38.0 2.0 40.0 60-61 26.77732286164617 32.5 19.5 37.5 2.0 39.5 62-63 26.203401282626352 32.0 18.0 37.0 2.0 39.0 64-65 25.674086166964813 31.0 15.0 36.0 2.0 39.0 66-67 25.295558437613273 31.0 13.5 36.0 2.0 39.0 68-69 24.59673392350698 31.0 8.5 35.5 2.0 38.5 70-71 23.980638595535176 30.0 7.5 35.0 2.0 38.0 72-73 23.486074408548756 30.0 6.5 34.5 2.0 37.5 74-75 22.73945376152996 29.0 2.0 34.0 2.0 36.5 76-77 21.946042579438494 28.5 2.0 34.0 2.0 36.0 78-79 21.422237264887706 28.0 2.0 34.0 2.0 36.0 80-81 20.84930456524345 27.5 2.0 33.5 2.0 35.0 82-83 20.097126095993154 26.0 2.0 33.0 2.0 35.0 84-85 19.377372021045346 25.5 2.0 32.5 2.0 35.0 86-87 18.589074199034386 24.5 2.0 32.0 2.0 34.0 88-89 17.79317445575174 22.5 2.0 32.0 2.0 34.0 90-91 16.985021323156175 19.5 2.0 31.0 2.0 34.0 92-93 16.134511249925744 14.5 2.0 31.0 2.0 34.0 94-95 15.054982582561646 4.5 2.0 30.5 2.0 34.0 96-97 13.948559775446125 2.0 2.0 30.0 2.0 34.0 98-99 12.903118989578704 2.0 2.0 29.0 2.0 34.0 100-101 11.547934860594687 2.0 2.0 27.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 3.9197964240050958 1101 2 3.703337295916924 1101 3 3.2720118905894893 1101 4 3.057190726040563 1101 5 2.5516335474052845 1101 6 2.809121841035587 1101 7 2.6455046973073593 1101 8 2.6184194742712172 1101 9 2.6650675422647936 1101 10-11 3.567782685764744 1101 12-13 3.5196052266853393 1101 14-15 3.0784082974738887 1101 16-17 2.917972543989741 1101 18-19 2.9432482494306136 1101 20-21 2.9742900207945233 1101 22-23 3.0500788242969854 1101 24-25 3.109142711355304 1101 26-27 2.860351865193355 1101 28-29 3.1584110258510556 1101 30-31 3.1466040705316907 1101 32-33 2.9578177598825732 1101 34-35 3.2746105284452476 1101 36-37 3.281084814277065 1101 38-39 3.272601381437852 1101 40-41 2.9834317811367157 1101 42-43 3.1193881799842664 1101 44-45 3.0928269316041153 1101 46-47 2.9592912269622182 1101 48-49 2.9442321588205047 1101 50-51 2.934881230798215 1101 52-53 3.046984421147908 1101 54-55 2.9450859532759885 1101 56-57 3.143387070795754 1101 58-59 2.9329421817537344 1101 60-61 3.0293876178773544 1101 62-63 3.070531197934475 1101 64-65 2.894692117450827 1101 66-67 2.711401333558001 1101 68-69 2.8933195368014637 1101 70-71 2.5586008610918753 1101 72-73 2.7043300000959114 1101 74-75 2.4732651847435605 1101 76-77 2.529253594740389 1101 78-79 2.0565974902246538 1101 80-81 2.2273841242612775 1101 82-83 1.9461258538786907 1101 84-85 1.7938295793925505 1101 86-87 1.7245083282221607 1101 88-89 1.8450325867687773 1101 90-91 1.5521770613287593 1101 92-93 1.1635353492099796 1101 94-95 1.5240270680404837 1101 96-97 1.6013779922322389 1101 98-99 1.047757422814172 1101 100-101 0.7168505675852685 1102 1 3.6136178987837866 1102 2 2.5278602243333452 1102 3 2.7589064578923193 1102 4 2.9395672965086383 1102 5 1.880421115104017 1102 6 2.490910256534111 1102 7 2.4275550171233746 1102 8 2.349656230469339 1102 9 2.574078976264616 1102 10-11 2.067974840620341 1102 12-13 3.120957482098053 1102 14-15 3.145096445664965 1102 16-17 3.075358619049986 1102 18-19 3.087758819421257 1102 20-21 3.2137343730037635 1102 22-23 3.093219062958031 1102 24-25 3.152639900255515 1102 26-27 2.8636885910357286 1102 28-29 3.283025561835398 1102 30-31 3.3007444378854096 1102 32-33 2.7745762469676265 1102 34-35 3.072681317272366 1102 36-37 3.114309675735967 1102 38-39 3.269332145567766 1102 40-41 3.051043716743223 1102 42-43 2.9225558370605533 1102 44-45 2.7192308360021613 1102 46-47 2.953042952279013 1102 48-49 2.772837837605568 1102 50-51 2.7883683600577207 1102 52-53 2.9262389664059754 1102 54-55 2.7699527661872985 1102 56-57 2.802187291636301 1102 58-59 2.9172602923488746 1102 60-61 2.9166849637629717 1102 62-63 2.7868143571724993 1102 64-65 2.830188600997321 1102 66-67 2.4716443367537053 1102 68-69 2.325030540221153 1102 70-71 2.432798619481705 1102 72-73 2.264620599931458 1102 74-75 2.311304723846977 1102 76-77 2.1316478678183763 1102 78-79 2.063698771355263 1102 80-81 2.0540996417482518 1102 82-83 2.0617016946298605 1102 84-85 1.968694231052929 1102 86-87 1.6898545851811448 1102 88-89 1.6037431156956465 1102 90-91 1.5047489990572238 1102 92-93 1.3265373856977867 1102 94-95 1.1612523067359142 1102 96-97 1.0189963624722065 1102 98-99 0.7888634822741132 1102 100-101 0.31026474480195 1103 1 3.922952538769291 1103 2 3.650770538767631 1103 3 3.208236538882673 1103 4 2.9698401932824687 1103 5 2.5806971875881395 1103 6 2.6840639706539484 1103 7 2.456258567489556 1103 8 2.5443294148105586 1103 9 2.907716762151601 1103 10-11 3.5662132955496055 1103 12-13 2.7494157429388117 1103 14-15 3.1587842441184115 1103 16-17 2.594739901062802 1103 18-19 2.5909501160267503 1103 20-21 2.895718841247632 1103 22-23 3.152108421600925 1103 24-25 3.071102661642769 1103 26-27 2.9745867325632993 1103 28-29 2.3552568977183874 1103 30-31 2.9535929331247637 1103 32-33 3.103588893308455 1103 34-35 2.6671409691911414 1103 36-37 2.968108643532709 1103 38-39 3.109170457102998 1103 40-41 3.195546133595144 1103 42-43 3.009279322314935 1103 44-45 2.9203072654127453 1103 46-47 2.857260120207844 1103 48-49 2.7603269028362156 1103 50-51 2.793692155869291 1103 52-53 2.8132386215589094 1103 54-55 2.9642660042233473 1103 56-57 3.133920625489175 1103 58-59 3.1255093459677212 1103 60-61 3.206851756743678 1103 62-63 3.2555938128596473 1103 64-65 3.38309729835213 1103 66-67 3.025189442555515 1103 68-69 3.1823907846144657 1103 70-71 2.8735005099060587 1103 72-73 2.654507326436562 1103 74-75 2.8095468805244437 1103 76-77 2.801404390201288 1103 78-79 2.6595703479687884 1103 80-81 2.6097621475200583 1103 82-83 2.68212735670701 1103 84-85 2.566174996574457 1103 86-87 2.3153184239719593 1103 88-89 2.289644812849783 1103 90-91 2.185638529149511 1103 92-93 2.1489718306596117 1103 94-95 2.052240303330306 1103 96-97 1.9730466594887925 1103 98-99 1.8293096598294412 1103 100-101 1.6423437050393304 1104 1 3.775519759515525 1104 2 3.545049730113888 1104 3 2.86691717128474 1104 4 2.899029349513068 1104 5 1.8279544946985489 1104 6 2.3523843796232846 1104 7 2.5811720036119254 1104 8 2.5152348831006357 1104 9 2.542001962077741 1104 10-11 3.352200810669938 1104 12-13 3.3803904353174694 1104 14-15 3.141414884904325 1104 16-17 3.114542744776628 1104 18-19 2.930624359922991 1104 20-21 2.9549405931558006 1104 22-23 2.4811496594841564 1104 24-25 2.7208530566673446 1104 26-27 2.649446971335852 1104 28-29 2.5924032351956576 1104 30-31 2.2437064748314306 1104 32-33 2.5013485844589525 1104 34-35 3.0341962189900364 1104 36-37 3.053323484995147 1104 38-39 3.0668782745664345 1104 40-41 3.1667716639237113 1104 42-43 3.0218579775005914 1104 44-45 3.035592019808668 1104 46-47 3.1016258706078865 1104 48-49 2.9170469803881893 1104 50-51 2.7722863006104603 1104 52-53 3.1139946722509464 1104 54-55 2.923429947186989 1104 56-57 3.048174051720739 1104 58-59 3.1096751268158656 1104 60-61 3.1995658230144137 1104 62-63 3.350475830322349 1104 64-65 3.25668731291821 1104 66-67 3.0914834801494635 1104 68-69 3.0171186590417065 1104 70-71 3.1406482134959077 1104 72-73 2.865984640188433 1104 74-75 2.9433415920093786 1104 76-77 2.8330002778937136 1104 78-79 2.713041885071192 1104 80-81 2.987807898398625 1104 82-83 2.945471411194063 1104 84-85 2.560036607055242 1104 86-87 2.585068157165157 1104 88-89 2.6470823792861715 1104 90-91 2.734376862442005 1104 92-93 2.732366491127575 1104 94-95 2.771945309843458 1104 96-97 2.6521143995700545 1104 98-99 2.489747522063933 1104 100-101 2.1742436878471505 1105 1 3.5601545395826086 1105 2 3.264980761202594 1105 3 2.9889619391972744 1105 4 2.5914089770952593 1105 5 -0.12250124523841777 1105 6 1.818744728358535 1105 7 2.1419823042017896 1105 8 2.2531422878992764 1105 9 2.68533241638864 1105 10-11 2.6474278181886213 1105 12-13 3.168383210076616 1105 14-15 3.0299175299276193 1105 16-17 2.9767605885728585 1105 18-19 2.9325742022793584 1105 20-21 2.926314414768502 1105 22-23 2.668781027169995 1105 24-25 2.038722078582566 1105 26-27 2.428747035313773 1105 28-29 2.511133730366563 1105 30-31 2.5978169521364727 1105 32-33 2.69335451602511 1105 34-35 2.8409710618709276 1105 36-37 2.9493357762583656 1105 38-39 3.2492682293081216 1105 40-41 3.0311921387526013 1105 42-43 2.9491619197953547 1105 44-45 2.9528178688213558 1105 46-47 3.225139848820973 1105 48-49 2.978787653530251 1105 50-51 3.0633524712975877 1105 52-53 3.083907501974835 1105 54-55 3.063428318974591 1105 56-57 3.3777520382117956 1105 58-59 3.246085920162354 1105 60-61 3.1414802159769017 1105 62-63 3.14484899320518 1105 64-65 3.1880039672588687 1105 66-67 3.0461868820208053 1105 68-69 3.226187173619863 1105 70-71 3.1782467703814845 1105 72-73 3.1194529308003673 1105 74-75 3.0172008450369674 1105 76-77 3.1208902741625515 1105 78-79 3.060159702578165 1105 80-81 3.088346436416657 1105 82-83 3.2851982176517254 1105 84-85 3.241084398473113 1105 86-87 3.154096869491834 1105 88-89 3.205595730482788 1105 90-91 2.9369202481627177 1105 92-93 2.7714363370884314 1105 94-95 2.8424729637837363 1105 96-97 2.8556439463555865 1105 98-99 2.9233206138623355 1105 100-101 2.596226305229516 1106 1 3.242675958630258 1106 2 2.7159187683681374 1106 3 2.126867408651261 1106 4 1.1820520873283478 1106 5 1.9422548466250724 1106 6 2.3874255919811738 1106 7 2.140950231115724 1106 8 0.6671094794524954 1106 9 1.8878287314363291 1106 10-11 1.0900766951510725 1106 12-13 1.7631575264405477 1106 14-15 1.7972276199359705 1106 16-17 1.9746931335244327 1106 18-19 2.085992078883553 1106 20-21 2.1983446908878115 1106 22-23 2.6019214283223597 1106 24-25 2.8553302275250587 1106 26-27 2.757585581213764 1106 28-29 2.323895977332594 1106 30-31 2.4309032556307564 1106 32-33 2.4684310819409347 1106 34-35 2.660161122046045 1106 36-37 1.7698313115965227 1106 38-39 2.8050159795438674 1106 40-41 2.1954263368681595 1106 42-43 2.2478320083339653 1106 44-45 2.4379526186105522 1106 46-47 2.7222421127541345 1106 48-49 2.5655706879436515 1106 50-51 2.6999335012826258 1106 52-53 2.613821077089238 1106 54-55 2.6588046532789598 1106 56-57 2.8049039659087995 1106 58-59 2.7545537790714754 1106 60-61 2.7996648992774382 1106 62-63 3.2742560830702097 1106 64-65 2.957135420814641 1106 66-67 2.6020314786208836 1106 68-69 2.917108891236804 1106 70-71 2.7924265103196078 1106 72-73 2.841953378486391 1106 74-75 3.131892818779704 1106 76-77 3.1192421080652615 1106 78-79 2.9035106835809685 1106 80-81 2.7987304705255447 1106 82-83 2.8443508509789446 1106 84-85 2.908670389500827 1106 86-87 3.025578188218308 1106 88-89 2.982327327179558 1106 90-91 2.81217398218023 1106 92-93 2.707495660993146 1106 94-95 2.818014836724272 1106 96-97 2.903851458927095 1106 98-99 2.656979283814568 1106 100-101 2.3168082466865076 1107 1 2.9827028616721805 1107 2 3.260886163337826 1107 3 -0.15493567564687183 1107 4 0.8579608829554672 1107 5 1.3329365152469315 1107 6 2.0089016919161047 1107 7 1.8750344010133375 1107 8 1.9059459044192373 1107 9 2.224083931433377 1107 10-11 1.5771048694646375 1107 12-13 2.1138971490063874 1107 14-15 2.5580242201682744 1107 16-17 1.0650453480648423 1107 18-19 0.23755826548554282 1107 20-21 -1.4179189842534718 1107 22-23 0.3688239847446013 1107 24-25 1.186887157960804 1107 26-27 1.2737671435005389 1107 28-29 2.0185736817400475 1107 30-31 2.671111746289892 1107 32-33 1.429192377867544 1107 34-35 0.9279622231815381 1107 36-37 0.8912809201092422 1107 38-39 2.0593673011861426 1107 40-41 2.336298960094169 1107 42-43 1.5548283296147574 1107 44-45 1.311293698837872 1107 46-47 2.191121077034513 1107 48-49 1.7634978660847445 1107 50-51 2.14887566501967 1107 52-53 1.6354675770288125 1107 54-55 2.2264419508794795 1107 56-57 2.6858240203903634 1107 58-59 2.666988018865286 1107 60-61 2.5343816970027646 1107 62-63 2.2953064732381954 1107 64-65 1.7475984560455977 1107 66-67 2.362177878030259 1107 68-69 2.5139207910570107 1107 70-71 1.1805635775714087 1107 72-73 2.0345544614683995 1107 74-75 2.48686922188838 1107 76-77 2.470890196434734 1107 78-79 2.2916970396541814 1107 80-81 2.3466470679292826 1107 82-83 2.206947601399669 1107 84-85 2.421015075622325 1107 86-87 2.639998545873617 1107 88-89 2.6156832468250606 1107 90-91 2.610757271581072 1107 92-93 2.4439441111657914 1107 94-95 2.4394091404352327 1107 96-97 1.9393759283994232 1107 98-99 2.0603595259630545 1107 100-101 1.644926411094806 1108 1 2.5539906751557346 1108 2 2.2559854735013865 1108 3 1.810269905412806 1108 4 1.5617408391641057 1108 5 1.9097209686456615 1108 6 -0.13340686566418825 1108 7 0.5114101870192584 1108 8 -0.5057484603944289 1108 9 1.443024140411275 1108 10-11 2.859929178347418 1108 12-13 1.8043040344516648 1108 14-15 2.0205917392740993 1108 16-17 0.12546940702949172 1108 18-19 0.2738070877295904 1108 20-21 1.0315119619383957 1108 22-23 -0.6954618886435711 1108 24-25 1.0324943231718393 1108 26-27 1.1609755444921923 1108 28-29 1.8028672063654447 1108 30-31 0.5470986111091314 1108 32-33 0.8036321781112221 1108 34-35 1.054296177969075 1108 36-37 -0.18229419009307435 1108 38-39 1.2765976758183406 1108 40-41 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2312 54-55 -1.0686777247687722 2312 56-57 -0.36751723829276983 2312 58-59 -0.56293311458872 2312 60-61 -0.19548511985859562 2312 62-63 -0.659783808391186 2312 64-65 -2.1205288110272242 2312 66-67 0.9130514579523279 2312 68-69 -0.07938085520868654 2312 70-71 -2.8053750174489487 2312 72-73 -3.324233862900549 2312 74-75 -0.9689024535133655 2312 76-77 -0.32919242497861134 2312 78-79 -0.5221999107342796 2312 80-81 -2.086201930510015 2312 82-83 -0.13766230084479503 2312 84-85 1.1478560713214279 2312 86-87 1.3599572423327189 2312 88-89 1.781313527107276 2312 90-91 2.269745664454849 2312 92-93 2.4434989014163193 2312 94-95 1.8407611076561015 2312 96-97 1.3516193039965128 2312 98-99 0.7540530685944145 2312 100-101 1.3645141352582382 2313 1 -7.7587234196425 2313 2 -4.333134144224367 2313 3 -1.8047857272325203 2313 4 0.2580813791111183 2313 5 0.8816385298097167 2313 6 -2.478414037004317 2313 7 -0.821747347496192 2313 8 -2.1190554921764644 2313 9 0.8051209196517632 2313 10-11 -1.1112771514340984 2313 12-13 -1.9060742402280155 2313 14-15 -3.3694916143984237 2313 16-17 -3.4655363499551015 2313 18-19 -0.8011011299264084 2313 20-21 -2.519576867099243 2313 22-23 -1.2026416204621917 2313 24-25 -2.310723284473646 2313 26-27 -1.5232850849358535 2313 28-29 -2.2278746162871137 2313 30-31 -4.134053465677976 2313 32-33 -5.3802090801076545 2313 34-35 -4.358073658367346 2313 36-37 -1.6377999502067695 2313 38-39 -1.5511923765856395 2313 40-41 -3.3044651580740876 2313 42-43 -2.1093222962369005 2313 44-45 -2.3640598873723846 2313 46-47 -0.8524439403292341 2313 48-49 -2.094750993833525 2313 50-51 -2.9340674818675474 2313 52-53 -0.17025507108634486 2313 54-55 -1.5927544161331433 2313 56-57 -1.9424920811912898 2313 58-59 -0.7893869278342613 2313 60-61 -1.1563280776821507 2313 62-63 -3.4386201245203196 2313 64-65 -4.893701530589514 2313 66-67 -3.4061928003899666 2313 68-69 -2.309405256407441 2313 70-71 -1.3207311903921735 2313 72-73 -1.2459303941306779 2313 74-75 -2.411113598923791 2313 76-77 -2.143964136865833 2313 78-79 -2.4904544672902595 2313 80-81 -3.517853590768894 2313 82-83 -1.399752168710073 2313 84-85 -1.124919204473251 2313 86-87 -2.5483684130056545 2313 88-89 -3.4054305930494095 2313 90-91 -3.1178801530823126 2313 92-93 -1.552017570491003 2313 94-95 -0.48011547960234324 2313 96-97 -0.6728337829967259 2313 98-99 -0.1740699169614377 2313 100-101 0.12362779630560006 2314 1 -7.846776496093366 2314 2 -3.9782231558879637 2314 3 -24.921657805430566 2314 4 -13.42791522097388 2314 5 -6.900792881627222 2314 6 -5.314299667641325 2314 7 -4.640314661091132 2314 8 -3.576405447316475 2314 9 -3.332688968434365 2314 10-11 -14.438567371956363 2314 12-13 -13.109260480016466 2314 14-15 -5.164263064556611 2314 16-17 -3.4092051887896844 2314 18-19 -3.560854636088756 2314 20-21 -4.520073868563099 2314 22-23 -4.414504726662312 2314 24-25 -5.11911210253097 2314 26-27 -3.8664958796659796 2314 28-29 -3.3643091915338452 2314 30-31 -3.728902830554965 2314 32-33 -4.238935528613112 2314 34-35 -3.457744291601518 2314 36-37 -4.068189051590345 2314 38-39 -1.0254592699133056 2314 40-41 -2.1992838292739734 2314 42-43 -4.502104560013638 2314 44-45 -5.499303311842333 2314 46-47 -4.665122790024661 2314 48-49 -3.3288233364694015 2314 50-51 -2.795685038650852 2314 52-53 -1.201724353798724 2314 54-55 -3.6251327038981707 2314 56-57 -3.2333772396178837 2314 58-59 -1.3646938646052824 2314 60-61 -4.0839748170692225 2314 62-63 -5.346464720072095 2314 64-65 -4.594645951423438 2314 66-67 -5.559932706892305 2314 68-69 -4.749610431833613 2314 70-71 -5.510880881094348 2314 72-73 -4.708153560773734 2314 74-75 -4.212821820107152 2314 76-77 -3.9636057291593616 2314 78-79 -4.916574786504501 2314 80-81 -7.032082471157997 2314 82-83 -6.49358392013851 2314 84-85 -5.0073152001289145 2314 86-87 -4.0219317128120515 2314 88-89 -3.14582819422049 2314 90-91 -5.25892822410276 2314 92-93 -5.307619166562212 2314 94-95 -5.788373259268964 2314 96-97 -5.278787175828571 2314 98-99 -6.068392767223514 2314 100-101 -5.051725180981078 2315 1 -24.37070812002499 2315 2 -24.52480435246916 2315 3 -13.32076094444402 2315 4 -28.724069067127726 2315 5 -15.513891268043993 2315 6 -12.337627635139697 2315 7 -9.374387713101402 2315 8 -8.579299165456831 2315 9 -5.990375527017786 2315 10-11 -29.045191303580292 2315 12-13 -28.794089539845526 2315 14-15 -23.610371876024168 2315 16-17 -14.151230216577044 2315 18-19 -9.171927419807279 2315 20-21 -6.950727158969752 2315 22-23 -4.066385643453543 2315 24-25 -5.833885396003815 2315 26-27 -5.7887363073996845 2315 28-29 -17.46464934714306 2315 30-31 -16.38022608378405 2315 32-33 -9.584267252195957 2315 34-35 -7.647887444412827 2315 36-37 -5.37393624410884 2315 38-39 -5.341809735591124 2315 40-41 -8.817476884350441 2315 42-43 -8.273330270028588 2315 44-45 -5.686300743907029 2315 46-47 -4.618578004466425 2315 48-49 -5.526044600506811 2315 50-51 -4.011931062183873 2315 52-53 -7.649454413972727 2315 54-55 -7.319733332467411 2315 56-57 -8.012052836567022 2315 58-59 -8.13600102032948 2315 60-61 -5.787710779661687 2315 62-63 -4.272298340860875 2315 64-65 -5.065099364477749 2315 66-67 -7.537097246388683 2315 68-69 -7.272180475143568 2315 70-71 -8.055342572865456 2315 72-73 -7.568871816110823 2315 74-75 -8.036541685194731 2315 76-77 -7.6647555509369845 2315 78-79 -7.458148509244143 2315 80-81 -8.520004551568867 2315 82-83 -7.957173855365216 2315 84-85 -7.1874137015278645 2315 86-87 -7.238795765550542 2315 88-89 -6.736846169767613 2315 90-91 -6.095802050326361 2315 92-93 -5.865427414617097 2315 94-95 -5.887488854685007 2315 96-97 -6.008622176403483 2315 98-99 -5.855908577272957 2315 100-101 -5.593657264841413 2316 1 -1.4293337807650168 2316 2 1.3973484586793248 2316 3 1.73812114699615 2316 4 1.8098088761687698 2316 5 -1.4931052971736598 2316 6 -2.8849673282417108 2316 7 -2.971517197675695 2316 8 -4.112849136042719 2316 9 -3.7238864942786947 2316 10-11 -3.2696026442818784 2316 12-13 -3.7727055898214985 2316 14-15 -2.23030677710441 2316 16-17 -2.4434627367305595 2316 18-19 -4.3186559889886205 2316 20-21 -1.8894741609931849 2316 22-23 -2.327253672147922 2316 24-25 -2.780960038886356 2316 26-27 -2.7480034758660317 2316 28-29 -2.0363941317951415 2316 30-31 0.7543055423098259 2316 32-33 -1.3244374446051737 2316 34-35 -6.702873297626727 2316 36-37 -2.8859445274669184 2316 38-39 -1.2050715125481304 2316 40-41 -1.1722132024796608 2316 42-43 -1.3508871965665783 2316 44-45 -0.8982422259902805 2316 46-47 0.179610388057867 2316 48-49 0.45713365330786715 2316 50-51 0.9779259184416063 2316 52-53 0.8158067682374437 2316 54-55 -1.1671688099780049 2316 56-57 -0.10663600738650558 2316 58-59 -3.3508166811341553 2316 60-61 -3.42055373417433 2316 62-63 -4.799945233631899 2316 64-65 -5.151680496780902 2316 66-67 -3.2768494998542685 2316 68-69 -2.952912078395709 2316 70-71 -3.5063473933316445 2316 72-73 -3.556128934217636 2316 74-75 -3.2193325993594044 2316 76-77 -2.9563871828490313 2316 78-79 -3.5256099315079297 2316 80-81 -3.659460724545383 2316 82-83 -4.271320318237715 2316 84-85 -3.459296290745277 2316 86-87 -3.0243810201388204 2316 88-89 -4.273366318971494 2316 90-91 -4.775322072392331 2316 92-93 -5.095998048345706 2316 94-95 -6.202139343950472 2316 96-97 -5.908527253946834 2316 98-99 -6.0964300150949775 2316 100-101 -5.666493927104943 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 39958.0 3 15071.0 4 13863.0 5 14282.0 6 16675.0 7 20230.0 8 24612.0 9 29020.0 10 34302.0 11 39255.0 12 43095.0 13 46006.0 14 50023.0 15 53646.0 16 56736.0 17 58817.0 18 58093.0 19 60099.0 20 64845.0 21 69971.0 22 75798.0 23 82483.0 24 89625.0 25 97185.0 26 104602.0 27 113096.0 28 122189.0 29 129886.0 30 139383.0 31 147315.0 32 156799.0 33 164592.0 34 167892.0 35 164527.0 36 142584.0 37 87517.0 38 17046.0 39 145.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.48408042270522 18.92998935514137 14.733568507852207 22.852361714301203 2 15.808571917157654 20.089207093834442 40.41253449728671 23.689686491721197 3 19.93239529712662 24.26311951520028 29.440590840676332 26.363894346996762 4 13.113117855370135 16.889706708356798 37.05816842654573 32.93900700972734 5 14.147796429477477 36.87645553094633 34.09644487011747 14.879303169458721 6 31.965685614775662 35.60341776631781 17.851270624912324 14.579625993994199 7 28.763609122571648 32.34533008330859 20.700713595485855 18.19034719863391 8 26.205317258282246 34.65468942897319 19.788115842635136 19.35187747010943 9 26.384200243617677 17.40345999767135 20.593954279492355 35.61838547921862 10-11 25.144953244976055 26.50175399218758 27.25017829538749 21.103114467448876 12-13 26.402742591466282 23.896890357270202 27.530106603858272 22.170260447405244 14-15 23.31010877117654 25.46756733421865 26.032721982202705 25.189601912402104 16-17 22.488486727916506 28.569007535474405 26.629134675465473 22.31337106114362 18-19 22.286483275203448 27.160153811122946 29.01581782080495 21.537545092868655 20-21 23.297101055697887 26.15866685352951 28.94344555729142 21.600786533481187 22-23 22.892412801855144 26.177725968915276 28.829429412849056 22.100431816380524 24-25 22.69671439376046 26.274714544098842 29.095304917953364 21.93326614418734 26-27 22.425023467739205 26.44266479518497 29.086477060396255 22.04583467667957 28-29 22.212968549873437 26.82400969710149 29.022596406793266 21.940425346231805 30-31 22.39479887198184 26.44253598466659 29.002118731270006 22.160546412081562 32-33 22.297733278748744 26.533953431364687 28.943549487592517 22.22476380229405 34-35 22.907401262383782 26.541639481616492 28.65426298100618 21.89669627499355 36-37 22.720088878313792 26.357022106307753 28.740426311001432 22.18246270437702 38-39 22.447534324453354 26.651682639884505 28.92264859343462 21.97813444222752 40-41 22.651863061427967 26.515870430939408 28.736535870185193 22.095730637447424 42-43 22.43390696267192 26.61221173919261 28.530498718642995 22.423382579492483 44-45 22.673120238890686 26.626470449957328 28.39927355462445 22.301135756527536 46-47 22.804190972919187 26.81109150561594 28.03987932260649 22.344838198858376 48-49 22.74587050277182 26.840587485987704 28.041267450909977 22.3722745603305 50-51 22.590185061829505 27.096520868960578 28.01574603111259 22.29754803809733 52-53 22.690321447141137 27.264721782989415 27.63515630095056 22.40980046891889 54-55 22.49776197698685 26.995172570105712 27.793448294540934 22.7136171583665 56-57 22.54902833983577 27.21286174733166 27.744961785202666 22.493148127629905 58-59 22.738327346352413 27.02629872425567 27.7110568842444 22.52431704514752 60-61 22.602227729559885 27.039720139559847 28.02228014463112 22.335771986249146 62-63 22.542431042155293 27.021421063062974 27.987098890402716 22.449049004379017 64-65 22.351952184665823 27.111227525797553 27.865348964794666 22.671471324741955 66-67 22.32494154821251 27.23681471722618 27.534936194026994 22.903307540534318 68-69 22.312617080105625 27.089476487118763 27.827749959181652 22.770156473593957 70-71 22.392570083738637 26.96090582466458 27.691392927661102 22.95513116393569 72-73 22.458475453056987 26.948339632581742 27.697338998708922 22.895845915652348 74-75 22.259484997101982 27.362775200376475 27.4557183931693 22.922021409352244 76-77 22.46247880154505 27.019752443734905 27.443872976203394 23.073895778516654 78-79 22.336818554415075 27.130260991612413 27.44453485063707 23.08838560333544 80-81 22.407634392893666 27.096304663086894 27.410734328400753 23.085326615618687 82-83 22.37968475071877 27.085514498434904 27.454488804376815 23.080311946469507 84-85 22.332406623010108 27.16717692733541 27.31765454414616 23.182761905508322 86-87 22.17556483003578 27.128050584489966 27.526574971977364 23.16980961349689 88-89 22.28525851907291 27.027348593848465 27.242153305018057 23.445239582060566 90-91 22.2264359251644 27.064376494377672 27.353708853235304 23.355478727222625 92-93 22.386892127507192 26.921662290714387 27.388782802498646 23.30266277927977 94-95 22.07758395323072 26.842847435831867 27.425342779807423 23.65422583112999 96-97 22.110176465829014 26.730807545641923 27.383033604103645 23.775982384425415 98-99 22.00144122961162 26.786961630960022 27.07126850129053 24.14032863813783 100-101 22.04353907795195 26.69075480975454 27.286338218352178 23.979367893941333 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2421.0 1 1476.0 2 487.5 3 444.5 4 422.5 5 410.0 6 531.5 7 902.5 8 1409.5 9 1812.5 10 1847.5 11 1740.5 12 1825.5 13 2008.0 14 2194.0 15 2372.5 16 2596.5 17 2860.5 18 3223.0 19 3695.5 20 4230.5 21 4903.5 22 5834.0 23 7143.0 24 8752.0 25 10875.5 26 13758.5 27 17293.5 28 21119.5 29 25310.5 30 30087.5 31 35612.5 32 41928.5 33 48185.5 34 54691.0 35 61439.0 36 67947.0 37 73953.0 38 78482.0 39 81663.5 40 84840.0 41 88747.5 42 92368.0 43 96688.0 44 100752.0 45 103635.0 46 105799.5 47 108764.5 48 112474.0 49 112700.5 50 109528.5 51 105391.5 52 101880.5 53 98797.5 54 94400.0 55 87953.0 56 80725.0 57 72784.0 58 64755.0 59 56574.0 60 47464.0 61 38432.0 62 30347.5 63 23559.5 64 17673.0 65 12796.5 66 9381.5 67 6981.0 68 5063.5 69 3677.0 70 2686.5 71 1928.0 72 1423.0 73 1080.0 74 779.0 75 560.0 76 383.0 77 242.0 78 161.5 79 123.0 80 82.5 81 55.5 82 41.5 83 23.0 84 19.0 85 17.5 86 9.0 87 6.5 88 6.5 89 5.5 90 5.0 91 2.5 92 1.5 93 1.5 94 0.5 95 2.0 96 1.5 97 0.0 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.1954096788525295 2 2.4421763456496244 3 0.9758247449633849 4 0.8889954443963443 5 0.16814506504727592 6 0.09408582548128723 7 0.09493953429472803 8 0.10443704484425684 9 0.09831879834793116 10-11 1.7427398290376959 12-13 1.3352005842213979 14-15 0.5034214159258668 16-17 0.09063541902696404 18-19 0.09794530074205081 20-21 0.12448141635983542 22-23 0.1399904598040098 24-25 0.15160445678686058 26-27 0.15003932396221914 28-29 0.5469427798110671 30-31 0.1815020508575683 32-33 0.16398323458175204 34-35 0.14632213350369566 36-37 0.1362021269443663 38-39 0.4210740866293904 40-41 0.12373442114807472 42-43 0.12441027395871534 44-45 0.12873217482675936 46-47 0.14027502940849007 48-49 0.1548592216381036 50-51 0.1677004250402755 52-53 0.1861796637312126 54-55 0.185503810920572 56-57 0.155303861645104 58-59 0.13949246299616933 60-61 0.11710039224362856 62-63 0.12245385792791354 64-65 0.11715374904446862 66-67 0.11309863218062485 68-69 0.1098972241302219 70-71 0.12375220674835474 72-73 0.1393501781939292 74-75 0.1484030487364576 76-77 0.16894541705987665 78-79 0.1705105498845181 80-81 0.16906991626183676 82-83 0.16232917375571052 84-85 0.14943461355269855 86-87 0.14788726632833712 88-89 0.14449021667485398 90-91 0.15236923759890128 92-93 0.14457914467625405 94-95 0.19526810547430104 96-97 0.19791815991602352 98-99 0.21412084177111854 100-101 0.23839818615334105 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2811263.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.6841234893716 #Duplication Level Percentage of deduplicated Percentage of total 1 83.87320914242088 59.28504272472769 2 9.999521703569673 14.136148538595405 3 2.9226941707935734 6.197642270701185 4 1.1538085213498408 3.262237760247256 5 0.5874558117352929 2.0761899570623243 6 0.35564030374969496 1.508287388882464 7 0.2234125915774331 1.1054206248497875 8 0.1513021779060376 0.8555729461856987 9 0.11337159646364933 0.7212214732161415 >10 0.5790380272948865 7.2150161648612325 >50 0.027977872009741653 1.3289669721212838 >100 0.01183508751400366 1.4493746439689092 >500 4.188534944692231E-4 0.20602278533760754 >1k 2.6178343403407433E-4 0.449608553798134 >5k 5.235668680681487E-5 0.20324719544483924 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5492 0.19535703347570116 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4517 0.16067511292966896 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2822 0.10038192798041308 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 3.557120056003298E-5 3.557120056003298E-5 9 0.0 0.0 0.0 7.114240112006596E-5 7.114240112006596E-5 10-11 0.0 0.0 0.0 7.114240112006596E-5 1.0671360168009894E-4 12-13 0.0 0.0 0.0 7.114240112006596E-5 1.600704025201484E-4 14-15 0.0 0.0 0.0 7.114240112006596E-5 1.956416030801814E-4 16-17 0.0 0.0 0.0 7.114240112006596E-5 2.312128036402144E-4 18-19 0.0 0.0 0.0 7.114240112006596E-5 3.3792640532031334E-4 20-21 0.0 0.0 0.0 8.892800140008244E-5 3.557120056003298E-4 22-23 0.0 0.0 1.778560028001649E-5 1.2449920196011544E-4 3.557120056003298E-4 24-25 0.0 0.0 3.557120056003298E-5 2.6678400420024735E-4 3.912832061603628E-4 26-27 0.0 0.0 3.557120056003298E-5 6.758528106406267E-4 3.912832061603628E-4 28-29 0.0 0.0 3.557120056003298E-5 0.0014939904235213852 3.912832061603628E-4 30-31 0.0 0.0 3.557120056003298E-5 0.004215187266363909 4.0906880644037927E-4 32-33 0.0 0.0 3.557120056003298E-5 0.011293856177810473 4.4464000700041224E-4 34-35 0.0 0.0 3.557120056003298E-5 0.02285449635982119 4.624256072804288E-4 36-37 0.0 1.778560028001649E-5 3.557120056003298E-5 0.04167166145607863 4.624256072804288E-4 38-39 0.0 3.557120056003298E-5 3.557120056003298E-5 0.06963062509626455 4.8021120756044526E-4 40-41 0.0 3.557120056003298E-5 3.557120056003298E-5 0.09935036316417212 4.979968078404617E-4 42-43 0.0 3.557120056003298E-5 3.557120056003298E-5 0.13134665806792178 5.157824081204782E-4 44-45 0.0 3.557120056003298E-5 3.557120056003298E-5 0.17250253711587996 5.335680084004947E-4 46-47 0.0 3.557120056003298E-5 3.557120056003298E-5 0.22306699871196684 5.335680084004947E-4 48-49 0.0 3.557120056003298E-5 3.557120056003298E-5 0.27756207796993737 5.335680084004947E-4 50-51 0.0 3.557120056003298E-5 3.557120056003298E-5 0.3363968436962319 5.691392089605277E-4 52-53 0.0 5.335680084004947E-5 3.557120056003298E-5 0.3952849662233665 5.869248092405441E-4 54-55 0.0 7.114240112006596E-5 3.557120056003298E-5 0.4634571720966697 6.047104095205606E-4 56-57 0.0 7.114240112006596E-5 3.557120056003298E-5 0.5469783510116272 6.047104095205606E-4 58-59 0.0 7.114240112006596E-5 3.557120056003298E-5 0.6360486372139498 6.224960098005772E-4 60-61 0.0 7.114240112006596E-5 3.557120056003298E-5 0.734456363563281 6.402816100805937E-4 62-63 0.0 7.114240112006596E-5 3.557120056003298E-5 0.833717798726053 6.402816100805937E-4 64-65 0.0 7.114240112006596E-5 3.557120056003298E-5 0.9374967763599493 6.758528106406267E-4 66-67 0.0 7.114240112006596E-5 3.557120056003298E-5 1.0608754855024236 6.758528106406267E-4 68-69 0.0 7.114240112006596E-5 7.114240112006596E-5 1.2022354365279948 6.758528106406267E-4 70-71 0.0 7.114240112006596E-5 7.114240112006596E-5 1.3567745173610581 6.936384109206431E-4 72-73 0.0 7.114240112006596E-5 7.114240112006596E-5 1.5159912110677656 8.35923213160775E-4 74-75 0.0 7.114240112006596E-5 7.114240112006596E-5 1.6787828104307565 8.537088134407915E-4 76-77 0.0 7.114240112006596E-5 7.114240112006596E-5 1.8638953381451682 8.537088134407915E-4 78-79 0.0 7.114240112006596E-5 7.114240112006596E-5 2.075188269471764 8.537088134407915E-4 80-81 0.0 7.114240112006596E-5 7.114240112006596E-5 2.30812627633914 8.71494413720808E-4 82-83 3.557120056003298E-5 7.114240112006596E-5 7.114240112006596E-5 2.5483385937210428 8.892800140008246E-4 84-85 3.557120056003298E-5 7.114240112006596E-5 7.114240112006596E-5 2.7865411382713035 8.892800140008246E-4 86-87 3.557120056003298E-5 7.114240112006596E-5 7.114240112006596E-5 3.035094902184534 8.892800140008246E-4 88-89 3.557120056003298E-5 7.114240112006596E-5 7.114240112006596E-5 3.313172762562592 8.892800140008246E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 13215 0.0 30.267027 1 GGTATCA 9175 0.0 27.32866 1 TATCAAC 15555 0.0 25.013777 2 ATCAACG 15750 0.0 24.949104 3 TCAACGC 15890 0.0 24.688501 4 CAACGCA 16090 0.0 24.420685 5 AACGCAG 16310 0.0 24.160194 6 GTACATG 9600 0.0 23.030602 1 GAGTACT 7170 0.0 21.852102 12-13 TACATGG 9900 0.0 21.724695 2 ACGCAGA 17835 0.0 21.65953 7 CGCAGAG 17870 0.0 21.075417 8 ACATGGG 10190 0.0 20.767868 3 AGAGTAC 12110 0.0 20.513012 10-11 GTACTTT 8000 0.0 19.868343 14-15 GCAGAGT 17630 0.0 19.327814 9 GTGTAGC 1590 0.0 18.329674 1 AGTACTT 7945 0.0 18.298952 12-13 CATGGGA 5355 0.0 17.535799 4 ATCGACG 165 0.0013470512 17.489634 3 >>END_MODULE