FastQCFastQC Report
Fri 27 May 2016
SRR939446_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939446_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2811263
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79840.2840004652713033No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71930.2558636456283172No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44860.15957240571230794No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT33010.11742053304866888No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA102250.029.666471
GTATCAA140800.025.9269871
ATCAACG167700.021.5615583
TATCAAC169850.021.4034272
TCAACGC169700.021.3108564
GTACATG105350.021.2233891
CAACGCA171250.021.1526815
AACGCAG174000.020.709386
TACATGG107900.020.2768062
GAGTACT88450.020.12543312-13
GTATAGG7800.020.0838831
ACATGGG108650.019.2168033
AGAGTAC148150.019.08871510-11
GTACTTT92550.018.95214814-15
ACGCAGA192800.018.5916297
CGCAGAG195300.018.377928
AGTACTT96000.017.92538612-13
TCGCGGA2250.017.90956582-83
GTACCGT2654.0884515E-717.8919146
GCAGAGT201600.017.262689
Produced by FastQC (version 0.11.5)