##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939446_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2811263 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.91028694220356 31.0 31.0 31.0 28.0 34.0 2 31.16750762913324 31.0 31.0 34.0 30.0 34.0 3 31.186902470526594 31.0 31.0 34.0 28.0 34.0 4 35.09673018853092 35.0 35.0 37.0 32.0 37.0 5 34.663527389646575 35.0 35.0 37.0 32.0 37.0 6 34.71297420412107 35.0 35.0 37.0 31.0 37.0 7 34.55538418141597 35.0 35.0 37.0 30.0 37.0 8 34.45540918796996 35.0 35.0 37.0 30.0 37.0 9 35.97239710407742 37.0 35.0 39.0 30.0 39.0 10-11 35.76516907169483 37.0 35.0 39.0 30.0 39.0 12-13 35.760290659394016 37.0 35.0 39.0 30.0 39.0 14-15 36.62101962712133 38.0 35.5 40.0 30.5 41.0 16-17 36.53247134828723 38.0 35.0 40.0 30.0 41.0 18-19 36.5012195586112 38.0 35.0 40.0 30.0 41.0 20-21 36.3618903674256 38.0 34.5 40.0 29.5 41.0 22-23 36.20582101354445 38.0 34.0 40.0 29.0 41.0 24-25 36.05230833970354 38.0 34.0 40.0 29.0 41.0 26-27 35.598683758865675 38.0 34.0 40.0 27.0 41.0 28-29 35.40125203511731 38.0 34.0 40.0 27.0 41.0 30-31 35.12858953431251 38.0 33.0 40.0 27.0 41.0 32-33 34.93094705120083 37.5 33.0 40.0 26.0 41.0 34-35 35.23555764793262 38.0 34.0 40.0 27.0 41.0 36-37 35.116806218415 38.0 33.5 40.0 26.5 41.0 38-39 34.94767814324024 38.0 33.0 40.0 26.0 41.0 40-41 34.81643873234201 38.0 33.0 40.0 25.5 41.0 42-43 34.67600398824301 38.0 33.0 40.0 25.0 41.0 44-45 34.33112181250918 37.0 33.0 40.0 24.0 41.0 46-47 33.98345601247553 37.0 33.0 40.0 24.0 41.0 48-49 33.63091749153317 37.0 32.0 40.0 23.0 41.0 50-51 33.39879121946257 37.0 31.0 40.0 22.5 41.0 52-53 33.30069402969413 37.0 31.0 39.0 22.5 40.5 54-55 32.9720657583442 36.5 31.0 39.0 21.0 40.0 56-57 32.480031395141616 36.0 30.5 39.0 19.5 40.0 58-59 32.019213250414495 35.5 30.0 39.0 18.0 40.0 60-61 31.645234721902575 35.0 29.0 38.5 15.5 40.0 62-63 31.48043619540399 35.0 29.0 38.0 15.5 40.0 64-65 31.02376849835821 34.5 29.0 38.0 13.0 40.0 66-67 30.135801950938067 34.0 28.0 37.5 9.5 39.5 68-69 26.607981714980063 31.0 19.5 36.0 6.0 39.0 70-71 25.265686988374977 30.0 14.5 35.0 2.0 38.0 72-73 26.3065679020426 31.0 20.5 35.0 4.0 38.0 74-75 27.08305715260365 31.0 23.0 35.0 2.0 37.5 76-77 26.748090093313934 30.5 23.5 34.0 2.0 36.5 78-79 27.893986083834918 32.0 26.0 35.0 2.0 37.0 80-81 27.92024954620041 32.5 26.0 35.0 2.0 36.5 82-83 27.600358628844045 32.0 26.0 35.0 2.0 36.0 84-85 27.23340576815474 32.0 26.0 35.0 2.0 36.0 86-87 26.795807436017192 32.0 25.0 34.0 2.0 35.0 88-89 26.322682723032315 32.0 24.5 34.0 2.0 35.0 90-91 25.73098194654858 31.0 23.5 34.0 2.0 35.0 92-93 25.17144500532323 31.0 20.5 34.0 2.0 35.0 94-95 24.307226324964972 31.0 18.0 34.0 2.0 35.0 96-97 23.645665311285356 30.5 6.5 34.0 2.0 35.0 98-99 22.792105719030914 30.0 2.0 34.0 2.0 35.0 100-101 21.356066650469913 28.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.22350437235365206 1101 2 0.27705919630563614 1101 3 0.3581642862865806 1101 4 0.3877286853617292 1101 5 0.5082201638412585 1101 6 0.35232548184116297 1101 7 0.47685096999077814 1101 8 0.6464374082317121 1101 9 0.6045014417136088 1101 10-11 0.6598399118145082 1101 12-13 0.677188446317075 1101 14-15 0.6789647948436368 1101 16-17 0.6547833635002362 1101 18-19 0.5545803620831649 1101 20-21 0.639925003135744 1101 22-23 0.7268138759507607 1101 24-25 0.6893222379658113 1101 26-27 0.694679919307184 1101 28-29 0.5550621339640287 1101 30-31 0.3955898899724559 1101 32-33 0.8651990422179878 1101 34-35 0.669539580191433 1101 36-37 0.6890178344142441 1101 38-39 0.7898309970945618 1101 40-41 0.8823363367457944 1101 42-43 0.5325947998503722 1101 44-45 0.7400998222825734 1101 46-47 0.6988863988316965 1101 48-49 0.8728686963400349 1101 50-51 0.6701622647471552 1101 52-53 0.32171065701456314 1101 54-55 0.48430493523748197 1101 56-57 0.6369282412320558 1101 58-59 0.6496398418213829 1101 60-61 0.5459958598689276 1101 62-63 0.1695554534819017 1101 64-65 -0.018479349232965347 1101 66-67 -0.17667206041354078 1101 68-69 -7.198449072573698 1101 70-71 -7.991123893651114 1101 72-73 -5.113835339900856 1101 74-75 -2.9095602639175056 1101 76-77 -0.10764291627652867 1101 78-79 0.11072543364967302 1101 80-81 -0.05671476300471667 1101 82-83 -0.18409899762830406 1101 84-85 -0.26241047390888994 1101 86-87 -0.4266476441921725 1101 88-89 -0.2921249034822928 1101 90-91 -0.3218302087564169 1101 92-93 -0.3842889687808686 1101 94-95 -0.18528115475449525 1101 96-97 -0.4953719986177063 1101 98-99 -0.5643299938890287 1101 100-101 -0.521638825633346 1102 1 0.3166429900764989 1102 2 0.3104210516020274 1102 3 0.3254080724445565 1102 4 0.2731338560563472 1102 5 0.48192176778416496 1102 6 0.44355099953443755 1102 7 0.49314277423252406 1102 8 0.6599274453561037 1102 9 0.5391397121627293 1102 10-11 0.5452202772427484 1102 12-13 0.5599092451154206 1102 14-15 0.6735508121395384 1102 16-17 0.657018981112067 1102 18-19 0.5011319388630753 1102 20-21 0.5936658829023997 1102 22-23 0.6626608259836217 1102 24-25 0.5277768846061974 1102 26-27 0.6058745114643997 1102 28-29 0.5026718886718697 1102 30-31 0.40874355786088756 1102 32-33 0.7495930421924513 1102 34-35 0.5419048433875844 1102 36-37 0.6516280950512794 1102 38-39 0.7390884109220437 1102 40-41 0.6506983737837402 1102 42-43 0.5481485860181721 1102 44-45 0.5850736367627576 1102 46-47 0.6072260276270285 1102 48-49 0.4549641077095927 1102 50-51 0.38833774653419795 1102 52-53 0.27282090144221627 1102 54-55 -0.07121897654810994 1102 56-57 0.1552661606743726 1102 58-59 0.3466777491803441 1102 60-61 0.31341087604392825 1102 62-63 0.02835906355686646 1102 64-65 -0.04905451704918917 1102 66-67 -0.09909597210090126 1102 68-69 -7.043395871417232 1102 70-71 -7.882636071808921 1102 72-73 -1.559477128277063 1102 74-75 0.07062135975172978 1102 76-77 0.5258123640033325 1102 78-79 0.10445525174111125 1102 80-81 -0.10472891723354749 1102 82-83 -0.20074996247948462 1102 84-85 -0.4473744546973357 1102 86-87 -0.3875062109487928 1102 88-89 -0.469396353652197 1102 90-91 -0.4326791481225243 1102 92-93 -0.491724899970567 1102 94-95 -0.4926008695872568 1102 96-97 -0.7149123594876698 1102 98-99 -0.6175481456461753 1102 100-101 -0.5842319927402855 1103 1 0.32200370595563754 1103 2 0.24149157769837615 1103 3 0.3337058332765537 1103 4 0.2547431813445513 1103 5 0.41874870715927415 1103 6 0.5153914113246998 1103 7 0.4224031454594197 1103 8 0.4258355134137233 1103 9 0.39959335166310694 1103 10-11 0.5520677320960274 1103 12-13 0.48382536350122507 1103 14-15 0.5894080298666253 1103 16-17 0.64078282126669 1103 18-19 0.6235712545462135 1103 20-21 0.6560213442473852 1103 22-23 0.5471998901427142 1103 24-25 0.6314916863386131 1103 26-27 0.7269390173492098 1103 28-29 0.5895157781571569 1103 30-31 0.5065515384403483 1103 32-33 0.5731371348842202 1103 34-35 0.5541511031606916 1103 36-37 0.6007264271214723 1103 38-39 0.6638082957392228 1103 40-41 0.6024972285797432 1103 42-43 0.41322015639717335 1103 44-45 0.5590573607604128 1103 46-47 0.5135873844930856 1103 48-49 0.5118128289504185 1103 50-51 0.30146921119906267 1103 52-53 0.24678046666215891 1103 54-55 0.10418514719938088 1103 56-57 0.29620574229889485 1103 58-59 0.37481853640581164 1103 60-61 0.33796125975935354 1103 62-63 -0.03189107720698203 1103 64-65 0.00698342392156448 1103 66-67 -0.18867594057759263 1103 68-69 -6.971672259524102 1103 70-71 -7.751396142025239 1103 72-73 -1.2472637945800464 1103 74-75 0.23197384216355488 1103 76-77 0.4161285907139778 1103 78-79 0.23690652991579597 1103 80-81 -0.12726818011806884 1103 82-83 -0.02821099183824316 1103 84-85 -0.2294870229117869 1103 86-87 -0.2481988080368822 1103 88-89 -0.1683244053052242 1103 90-91 -0.28782798762446404 1103 92-93 -0.23063553392416836 1103 94-95 0.05600133080980285 1103 96-97 0.02420888445558589 1103 98-99 0.23933048182352223 1103 100-101 0.08841384152660225 1104 1 0.24388136208465028 1104 2 0.3386147530070893 1104 3 0.21310674660384876 1104 4 0.26128508540060835 1104 5 0.5295664856865727 1104 6 0.3655878093026246 1104 7 0.38957196783758263 1104 8 0.5020733744519035 1104 9 0.5970868245200123 1104 10-11 0.42174585050364044 1104 12-13 0.37095421936842854 1104 14-15 0.5979707385822906 1104 16-17 0.5830824614658283 1104 18-19 0.43598803118044316 1104 20-21 0.469790823169987 1104 22-23 0.5842950562845814 1104 24-25 0.5309522648255438 1104 26-27 0.6608946136737472 1104 28-29 0.6335304588505295 1104 30-31 0.6416917164479017 1104 32-33 0.6028563543708501 1104 34-35 0.2825927661982206 1104 36-37 0.6946676503209233 1104 38-39 0.7266721944585726 1104 40-41 0.556118719691618 1104 42-43 0.5291805383607056 1104 44-45 0.7283040924614923 1104 46-47 0.8587428174243925 1104 48-49 0.7919836084585015 1104 50-51 0.7659730045733752 1104 52-53 0.5883202839233661 1104 54-55 0.3975735964860192 1104 56-57 0.4973942795338644 1104 58-59 0.6115995641894187 1104 60-61 0.7744599865540103 1104 62-63 0.6071507704101045 1104 64-65 0.422701513856925 1104 66-67 0.2944450921726407 1104 68-69 -6.650976657383932 1104 70-71 -4.261935613706289 1104 72-73 0.41454688537161033 1104 74-75 1.1403058071331884 1104 76-77 1.2201526991277447 1104 78-79 0.7973309625690526 1104 80-81 0.49558407577179864 1104 82-83 0.36324572024137325 1104 84-85 0.2899189149312491 1104 86-87 0.38386612128705266 1104 88-89 0.44254686965020795 1104 90-91 0.458903568746301 1104 92-93 0.4560148545206779 1104 94-95 0.5800081061879006 1104 96-97 0.5283822811883816 1104 98-99 0.3706209912580931 1104 100-101 0.3273726900866549 1105 1 0.14432535936154878 1105 2 0.1937560608619293 1105 3 0.20364745138196483 1105 4 0.28562657065711505 1105 5 0.46674949066856897 1105 6 0.28437530157610524 1105 7 0.2643261768971499 1105 8 0.4042498129229273 1105 9 0.5235541808435613 1105 10-11 0.4355274501759112 1105 12-13 0.34299755147610256 1105 14-15 0.37809655956536403 1105 16-17 0.3570155641744108 1105 18-19 0.47158733855204105 1105 20-21 0.5308723313471546 1105 22-23 0.37613327423898824 1105 24-25 0.43039777342001884 1105 26-27 0.6226727627733339 1105 28-29 0.4949555159757395 1105 30-31 0.4360121848084191 1105 32-33 0.5679903227005738 1105 34-35 0.5143219558019041 1105 36-37 0.6475869316913929 1105 38-39 0.5077933134465766 1105 40-41 0.5371174133509058 1105 42-43 0.5756245234550192 1105 44-45 0.6503055638472617 1105 46-47 0.6686564651219626 1105 48-49 0.7965101646123429 1105 50-51 0.6724681700685053 1105 52-53 0.466110035873335 1105 54-55 0.6529208093953898 1105 56-57 0.7795756219795038 1105 58-59 0.7874416202387877 1105 60-61 0.9651556132277257 1105 62-63 0.6391380374797713 1105 64-65 0.7128179745524648 1105 66-67 0.7467616255576175 1105 68-69 -2.9279396467818906 1105 70-71 -3.4240180180770885 1105 72-73 0.7120588047275298 1105 74-75 1.2847444417513998 1105 76-77 1.1753072918034384 1105 78-79 0.7485446817861465 1105 80-81 0.601585778820084 1105 82-83 0.6590438106885728 1105 84-85 0.6386178581691944 1105 86-87 0.6991002720558441 1105 88-89 0.7285597806783599 1105 90-91 0.7149690224978649 1105 92-93 0.7949114080185247 1105 94-95 0.6840735030873937 1105 96-97 0.6264514281974911 1105 98-99 0.7521813988565107 1105 100-101 0.9654194308047579 1106 1 0.16237631664909458 1106 2 0.20795805422777747 1106 3 0.123242547647056 1106 4 0.1729538016323886 1106 5 0.18633891739636965 1106 6 0.3254803358782894 1106 7 0.3502707321807179 1106 8 0.35949237182808247 1106 9 0.3941783030653525 1106 10-11 0.2944126670352176 1106 12-13 0.27421198908120914 1106 14-15 0.28163432120241083 1106 16-17 0.290527596582038 1106 18-19 0.2670707322896462 1106 20-21 0.21222739619472009 1106 22-23 0.2768827890441301 1106 24-25 0.21769910626501598 1106 26-27 0.4924942108699568 1106 28-29 0.48029349003277133 1106 30-31 0.33358152379271644 1106 32-33 0.521158660646087 1106 34-35 0.3432209010407945 1106 36-37 0.5731332148207784 1106 38-39 0.34850010720175106 1106 40-41 0.3534261336820137 1106 42-43 0.3391060460477817 1106 44-45 0.44188408846969196 1106 46-47 0.6216360983463645 1106 48-49 0.6700795324381872 1106 50-51 0.5652564297408205 1106 52-53 0.28637409802963987 1106 54-55 0.2257865125765406 1106 56-57 0.32957733568110825 1106 58-59 0.5773071840520458 1106 60-61 0.8046537287979909 1106 62-63 0.4639333834810664 1106 64-65 0.5055226036111371 1106 66-67 0.687353764279937 1106 68-69 -3.191456036041263 1106 70-71 -3.283418925775937 1106 72-73 0.8639009154231694 1106 74-75 1.336750050444877 1106 76-77 1.3225661149714618 1106 78-79 1.0138109233560435 1106 80-81 0.7373620604589952 1106 82-83 0.5886793216674739 1106 84-85 0.4582258244814774 1106 86-87 0.5521739662621421 1106 88-89 0.5709118267442399 1106 90-91 0.8064259187541225 1106 92-93 0.5221862678777391 1106 94-95 1.018082044729308 1106 96-97 1.1507800646056197 1106 98-99 1.0675747457236398 1106 100-101 1.0717504174040045 1107 1 0.06971649870110141 1107 2 0.058730817457263385 1107 3 0.16329842238378944 1107 4 -0.09236982380845404 1107 5 0.22040308679792986 1107 6 0.12295731815700606 1107 7 0.113316570256913 1107 8 -0.028378356364122226 1107 9 0.06549025644889639 1107 10-11 -0.005189697772770785 1107 12-13 -0.01780031940349147 1107 14-15 -0.06989793315957371 1107 16-17 -0.012610528743500993 1107 18-19 0.2295928634454114 1107 20-21 0.13081139273710818 1107 22-23 0.2610665656338398 1107 24-25 0.1647565266865314 1107 26-27 0.42685939037573206 1107 28-29 0.34417090546207163 1107 30-31 0.21159564868130332 1107 32-33 0.16930418193341978 1107 34-35 0.10105547530200454 1107 36-37 0.3383263357055739 1107 38-39 0.493955429840355 1107 40-41 0.2071054069938043 1107 42-43 -0.007111195224162259 1107 44-45 0.2847797170156312 1107 46-47 0.48748385288170226 1107 48-49 0.43442988835730745 1107 50-51 0.32581629586942285 1107 52-53 0.18635372813128015 1107 54-55 0.2307083233021885 1107 56-57 0.5096806944451515 1107 58-59 0.5764191936902847 1107 60-61 0.4368529970648005 1107 62-63 0.2362888983671887 1107 64-65 0.16156388801238464 1107 66-67 0.2725779360667815 1107 68-69 -2.99669653540985 1107 70-71 -2.8869095136691456 1107 72-73 1.0814729079739074 1107 74-75 1.303239931724253 1107 76-77 0.9304123254051753 1107 78-79 0.8630216953382508 1107 80-81 0.6946269108273135 1107 82-83 0.644625300274857 1107 84-85 0.5748513027914903 1107 86-87 0.5640937869648184 1107 88-89 0.5775116210399318 1107 90-91 0.6052547551421483 1107 92-93 0.6296759523184612 1107 94-95 0.7837439371350818 1107 96-97 0.6440364144992579 1107 98-99 0.7764493193938016 1107 100-101 0.8623778894310163 1108 1 -0.12228723447898204 1108 2 -0.061248489676987816 1108 3 -0.08793390570838966 1108 4 -0.015609203958007356 1108 5 -0.07914674870603733 1108 6 -0.22200233186850227 1108 7 0.1251386784344959 1108 8 0.23637828528845972 1108 9 0.12916837435590622 1108 10-11 0.12629347284647707 1108 12-13 -0.011396668192986681 1108 14-15 -0.03520280210846494 1108 16-17 0.13895970115000722 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2312 20-21 0.20221191526559323 2312 22-23 0.2542422375329565 2312 24-25 0.43652744824849776 2312 26-27 0.502234124704529 2312 28-29 0.25423402162935105 2312 30-31 0.02389061787562241 2312 32-33 0.6634123364447788 2312 34-35 0.43416910590045177 2312 36-37 0.5654302437772429 2312 38-39 0.72241904417249 2312 40-41 0.437067815325598 2312 42-43 0.17979814765971724 2312 44-45 0.7512371429823972 2312 46-47 0.7518012910876166 2312 48-49 0.7669302000686287 2312 50-51 0.9005390161131785 2312 52-53 0.7503151705162807 2312 54-55 0.9613113521329737 2312 56-57 0.7894820161011324 2312 58-59 0.9531511979152185 2312 60-61 0.8934723839322238 2312 62-63 0.937460502971053 2312 64-65 1.0277185598842742 2312 66-67 1.4968637588628404 2312 68-69 4.256559307206754 2312 70-71 5.081518572738663 2312 72-73 3.3702051529689747 2312 74-75 2.587330687728098 2312 76-77 2.4820284948385485 2312 78-79 1.7872396582223864 2312 80-81 1.682726632651832 2312 82-83 1.5586632015046504 2312 84-85 1.5407760965245885 2312 86-87 1.531947751287845 2312 88-89 1.621113800009521 2312 90-91 1.790377669721785 2312 92-93 1.8992083566804148 2312 94-95 2.0681406001758624 2312 96-97 2.1484566959423326 2312 98-99 2.2248299253606056 2312 100-101 2.339840731292341 2313 1 -0.2656517393891171 2313 2 -0.15703395481278193 2313 3 -0.09511491761548996 2313 4 0.03235699146542714 2313 5 0.05331225574924048 2313 6 0.16102858767310124 2313 7 -2.9487431927248835 2313 8 -3.159836692201896 2313 9 -0.8792565990893664 2313 10-11 0.09375426373634355 2313 12-13 0.09533284228410821 2313 14-15 -0.5398056447013957 2313 16-17 -0.14917000993117568 2313 18-19 0.11731137763730004 2313 20-21 -2.6243356811650784 2313 22-23 -0.46983560690335935 2313 24-25 0.13374658646083049 2313 26-27 -1.4161965590158871 2313 28-29 -1.4114280997375133 2313 30-31 -0.6950824123205805 2313 32-33 -0.7940748159543602 2313 34-35 -1.123376322388907 2313 36-37 -2.1169741162257267 2313 38-39 -1.9125048329459204 2313 40-41 -0.7373981933945615 2313 42-43 0.13934884127824887 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5 -0.2216912858288822 2314 6 -2.5771614297986787 2314 7 -0.7927225099086215 2314 8 -0.10729383910656054 2314 9 -0.041153940544717216 2314 10-11 -0.24155999229614622 2314 12-13 -2.3130571562992586 2314 14-15 -0.5649602936129483 2314 16-17 -1.8499137413378932 2314 18-19 -0.33597520686475946 2314 20-21 -0.6129258969652369 2314 22-23 -0.471110575029158 2314 24-25 -0.0931800320403795 2314 26-27 -2.017351113485354 2314 28-29 -0.8228331618331808 2314 30-31 -0.22999528949865322 2314 32-33 0.18209980745783128 2314 34-35 0.044435454538884755 2314 36-37 -0.30202983705493125 2314 38-39 -0.09901142028123644 2314 40-41 -0.20367292541514814 2314 42-43 -0.30812837456912945 2314 44-45 -3.499658967797437 2314 46-47 -3.95987133860827 2314 48-49 -3.7441858802334984 2314 50-51 -4.065821217107526 2314 52-53 -0.9163365885773374 2314 54-55 -1.0734762977709522 2314 56-57 -0.7172313609610761 2314 58-59 -1.4091906161707755 2314 60-61 -0.6418800973504943 2314 62-63 -0.91866154036029 2314 64-65 -0.9883948701826455 2314 66-67 -0.50315446150422 2314 68-69 -0.9117505052891097 2314 70-71 1.941698788732836 2314 72-73 1.4947943665116732 2314 74-75 1.1578551030199549 2314 76-77 1.0242864952360833 2314 78-79 -1.1423088079539845 2314 80-81 -0.7031012345713812 2314 82-83 0.3820515272185574 2314 84-85 0.34085063438982743 2314 86-87 0.16089825814533043 2314 88-89 0.0691857538954288 2314 90-91 0.3580812409137266 2314 92-93 -0.2149505249203294 2314 94-95 0.6005065976581427 2314 96-97 0.9392338774171876 2314 98-99 0.8032835131165186 2314 100-101 0.8890257839657636 2315 1 -0.38868616352851504 2315 2 -0.3351515758632857 2315 3 -0.4849395666180989 2315 4 -0.19288242239228026 2315 5 -0.22142107771114183 2315 6 -0.16828672204504613 2315 7 -0.8998341576032303 2315 8 -2.5589783280107774 2315 9 -0.6624333089092929 2315 10-11 -1.3167545038135273 2315 12-13 -0.5730117383390407 2315 14-15 -0.31747073782742774 2315 16-17 -0.28199376893361716 2315 18-19 0.03330155523711653 2315 20-21 -0.6385487170947854 2315 22-23 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0.10545385202827617 2315 88-89 0.19438409815206725 2315 90-91 0.1954791942309626 2315 92-93 -0.7916953794678747 2315 94-95 0.23792754741092992 2315 96-97 0.23837917656248564 2315 98-99 -1.9183810455435584 2315 100-101 -3.4519021222087787 2316 1 -0.4965669900542231 2316 2 -0.44615396131526097 2316 3 -0.9102055326615002 2316 4 -0.42237755559617796 2316 5 -0.2596753664607334 2316 6 -0.5364608708136416 2316 7 -0.41615737233915695 2316 8 -0.9600630945187731 2316 9 -5.008832612452128 2316 10-11 -1.4546070152182295 2316 12-13 -0.6156628439001395 2316 14-15 -0.4077105328552548 2316 16-17 -0.5059093795295908 2316 18-19 -0.574935149693033 2316 20-21 -3.571713071934539 2316 22-23 -1.077383446799388 2316 24-25 -0.28854499464464567 2316 26-27 -0.5463429689788981 2316 28-29 0.06221275626175782 2316 30-31 0.14442344409836494 2316 32-33 -1.3509400689986748 2316 34-35 -0.4457124855129422 2316 36-37 -0.2796645828420026 2316 38-39 -5.213015412444765 2316 40-41 -4.977520968556796 2316 42-43 -3.509980192194611 2316 44-45 -2.167982977868199 2316 46-47 -2.5333136966133374 2316 48-49 -3.020143260191361 2316 50-51 -1.2786299218311754 2316 52-53 -0.9460622916211605 2316 54-55 -0.09624251388171245 2316 56-57 -3.5003908585727572 2316 58-59 -0.7749030859317827 2316 60-61 -0.6969975211728645 2316 62-63 -0.24096693265607527 2316 64-65 0.030001323297643268 2316 66-67 0.04637988876295296 2316 68-69 2.6635787865947265 2316 70-71 3.6417585479708734 2316 72-73 2.2828448805232675 2316 74-75 0.8857839217584811 2316 76-77 -1.7365223285953242 2316 78-79 0.04268741345501681 2316 80-81 0.02434304147933375 2316 82-83 0.2056648459303858 2316 84-85 0.2546680586229151 2316 86-87 0.3030020297541469 2316 88-89 0.21725620818885005 2316 90-91 0.21372510049799942 2316 92-93 0.17342907561688747 2316 94-95 0.29843905123691883 2316 96-97 0.6067597628190953 2316 98-99 0.7085419153475279 2316 100-101 0.7841538658790483 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9.0 3 29.0 4 67.0 5 151.0 6 429.0 7 1103.0 8 2387.0 9 4086.0 10 6232.0 11 9054.0 12 12533.0 13 15796.0 14 19255.0 15 24215.0 16 28682.0 17 32818.0 18 32156.0 19 30435.0 20 30121.0 21 31206.0 22 33270.0 23 36974.0 24 44147.0 25 54048.0 26 68726.0 27 85572.0 28 105916.0 29 126073.0 30 147911.0 31 172410.0 32 199257.0 33 229962.0 34 264073.0 35 295251.0 36 305263.0 37 250320.0 38 104567.0 39 6759.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.961084150454695 20.21809668834588 15.680120113282195 23.14069904791723 2 16.0277631218961 21.03758424266013 39.087882754211556 23.846769881232213 3 19.543953432781453 24.43637131978395 30.16763373419919 25.852041513235402 4 13.53560318168969 17.7057143528497 36.56208901810082 32.19659344735978 5 13.882728154569671 37.411832333011894 34.16026888981927 14.545170622599166 6 31.98903837883542 36.500391461062165 17.159013582151513 14.351556577950909 7 28.3871697525276 32.64486460356075 20.521986025498148 18.445979618413503 8 26.031644851442216 35.610613450253496 19.265042082508824 19.092699615795464 9 25.653451847087943 17.31791013505318 20.377744807227213 36.650893210631665 10-11 24.963215832702478 26.871136595194862 27.10865061159904 21.056996960503625 12-13 25.879773610651156 24.098510171406947 27.669325139625855 22.352391078316046 14-15 22.771241964910434 25.725501313822292 25.93119178106068 25.57206494020659 16-17 22.198494158206763 28.85464000641707 26.60130194683756 22.34556388853861 18-19 21.968441197185605 27.45381074678258 28.862609648342737 21.71513840768908 20-21 22.89849793491395 26.458054618155614 28.89560670773243 21.747840739198004 22-23 22.53331071000954 26.411005322172493 28.804880235111824 22.25080373270614 24-25 22.470488611375977 26.541743169769212 28.900270902355096 22.087497316499714 26-27 22.321420313508263 26.65513877930558 28.87899417819159 22.14444672899457 28-29 22.195636352253427 26.96757571251831 28.806719915083313 22.030068020144945 30-31 22.352092906449712 26.609990972013243 28.716663500824186 22.321252620712862 32-33 22.296513163476174 26.694867700052416 28.70851325809619 22.300105878375216 34-35 22.646054955472515 26.747794969025517 28.580509642730732 22.025640432771233 36-37 22.56307686994823 26.526507514006152 28.712736811394112 22.197678804651506 38-39 22.362368539020196 26.87677160994726 28.71039791948323 22.050461931549318 40-41 22.556129336344554 26.696322027961074 28.618307697127282 22.129240938567087 42-43 22.30312627327314 26.778728646614226 28.368111731370227 22.550033348742403 44-45 22.564538984873693 26.750043298627386 28.318459312907297 22.366958403591624 46-47 22.715748102975965 26.97108050715467 27.9735885126584 22.33958287721097 48-49 22.611879954883317 26.981103429298543 27.97145268132597 22.435563934492173 50-51 22.509057414798896 27.190381474905777 27.93665371796754 22.363907392327782 52-53 22.720907399797944 27.214661961150842 27.597419642331207 22.467010996720006 54-55 22.54737013306504 27.018134135554128 27.77123041254807 22.663265318832764 56-57 22.607429119784122 27.200610856291146 27.63512722555321 22.556832798371524 58-59 22.751753655186498 27.046254228812444 27.611750321413766 22.5902417945873 60-61 22.644808272710275 27.052466300150435 27.97671405810386 22.32601136903543 62-63 22.52149397702649 27.016771696159765 27.97211292210482 22.489621404708924 64-65 22.430397425603875 27.108585458831115 27.78571268489865 22.675304430666362 66-67 22.275404876185696 27.150539549890553 27.749855350465157 22.82420022345859 68-69 22.115494357294935 26.739651158686446 28.54316315065531 22.601691333363313 70-71 21.7383415737193 26.605367766481518 28.944967382428686 22.711323277370493 72-73 22.311182179114933 26.887874753710456 28.005035927303574 22.79590713987104 74-75 22.330922587603744 27.25445299277168 27.494477635871156 22.92014678375342 76-77 21.71631636680214 27.12022234321159 28.066998803274558 23.096462486711715 78-79 22.354062883281436 27.12124567634081 27.52215750259931 23.00253393777844 80-81 22.404588129309264 27.132712654084113 27.443188639595473 23.019510577011154 82-83 22.453853648541788 27.143438487005106 27.463424229844772 22.93928363460833 84-85 22.30557554462253 27.20242069478479 27.33298497221352 23.159018788379157 86-87 22.196402889725782 27.21909603760101 27.483716034534705 23.100785038138504 88-89 22.257319296163754 27.127731947046342 27.19800316620664 23.416945590583264 90-91 22.218491023988026 27.106344900620975 27.342744371794776 23.332419703596223 92-93 22.290883950532965 27.014723111694273 27.385784585492388 23.308608352280377 94-95 21.972904597204593 26.884527076445874 27.551365690394146 23.591202635955387 96-97 21.893383000536264 26.77893978693823 27.656929062323965 23.670748150201536 98-99 21.877092332661416 26.840612368180818 27.31023765697164 23.972057642186122 100-101 21.788958635841396 26.604895876747296 27.509863030219588 24.096282457191716 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 745.0 1 526.5 2 322.5 3 348.5 4 339.5 5 360.0 6 560.0 7 1062.5 8 1623.5 9 2033.0 10 2164.5 11 2081.5 12 2120.0 13 2253.5 14 2433.0 15 2618.5 16 2895.0 17 3164.5 18 3405.5 19 3743.0 20 4178.5 21 4909.5 22 5828.5 23 7059.5 24 8640.5 25 10802.0 26 13858.0 27 17422.5 28 21156.5 29 25423.5 30 30382.5 31 36131.5 32 42381.5 33 48567.0 34 55244.0 35 61700.5 36 68463.5 37 74462.0 38 78173.5 39 80792.5 40 83620.0 41 87532.0 42 91104.5 43 95257.5 44 99747.5 45 102578.5 46 104775.5 47 108516.0 48 113730.5 49 113916.0 50 109215.5 51 105191.5 52 102201.5 53 99702.0 54 96514.5 55 90322.5 56 82152.0 57 74227.0 58 66369.5 59 57939.0 60 48281.5 61 38339.0 62 29751.5 63 22815.0 64 16629.0 65 11649.5 66 8374.0 67 6172.0 68 4406.5 69 3041.0 70 2094.5 71 1509.5 72 1122.5 73 842.5 74 617.5 75 380.0 76 237.5 77 144.5 78 81.0 79 55.5 80 38.5 81 24.0 82 11.5 83 7.0 84 7.5 85 5.0 86 3.0 87 2.0 88 1.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02073800992649923 2 0.017216461071055962 3 5.335680084004947E-4 4 0.08682930056704051 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.009639795351768938 12-13 0.0 14-15 0.0 16-17 4.0906880644037927E-4 18-19 1.0671360168009894E-4 20-21 0.0 22-23 1.778560028001649E-4 24-25 0.003646048057403381 26-27 0.003877260861043595 28-29 0.0013161344207212203 30-31 1.778560028001649E-4 32-33 6.580672103606101E-4 34-35 0.0027745536436825724 36-37 0.08567323654883943 38-39 0.09710937752889004 40-41 0.07879020924047304 42-43 0.08222283009451624 44-45 0.08119126527827528 46-47 0.08031977086455448 48-49 0.07478844917746934 50-51 0.07471730677634927 52-53 0.058781408925454505 54-55 0.04967518158208605 56-57 0.02781667883794579 58-59 0.022534355554780894 60-61 0.013605984214212614 62-63 0.002436627238362259 64-65 0.006971955309766464 66-67 0.15932340730838773 68-69 0.7336382259504002 70-71 0.8959673997061107 72-73 0.3624705337067361 74-75 0.05518871766889117 76-77 0.20241791678686769 78-79 0.014957689835493868 80-81 0.0033970496534831496 82-83 0.0015651328246414512 84-85 1.4228480224013192E-4 86-87 0.002205414434722045 88-89 4.4464000700041224E-4 90-91 0.004677612873644337 92-93 0.004926611277564568 94-95 0.08047984126707462 96-97 0.038843751011556016 98-99 0.014406336226813359 100-101 0.013783840217012781 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2811263.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.20720323944728 #Duplication Level Percentage of deduplicated Percentage of total 1 76.08389425127265 44.28630695932442 2 13.833570137767232 16.104268570723317 3 4.538317333616922 7.924882782088398 4 1.9729684317006053 4.593638979560429 5 1.0217175450860392 2.9735660400066113 6 0.5884742857814812 2.055206541220276 7 0.37659023460358076 1.5344185026493231 8 0.2813122361260007 1.3099518801543606 9 0.19187334974371506 1.0051569958289377 >10 1.0077329916487952 10.685083685102539 >50 0.07216174963065854 2.8427801220584534 >100 0.02953745651005196 3.0475606339369703 >500 0.0011099979073713536 0.40650378925509695 >1k 6.166655040419829E-4 0.6859055989674037 >5k 1.233331008083966E-4 0.54476891912336 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7984 0.2840004652713033 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7193 0.2558636456283172 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4486 0.15957240571230794 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3301 0.11742053304866888 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.557120056003298E-5 2 0.0 0.0 0.0 0.0 3.557120056003298E-5 3 0.0 0.0 0.0 0.0 3.557120056003298E-5 4 0.0 0.0 0.0 0.0 3.557120056003298E-5 5 0.0 0.0 0.0 0.0 7.114240112006596E-5 6 0.0 0.0 0.0 0.0 7.114240112006596E-5 7 0.0 0.0 0.0 0.0 7.114240112006596E-5 8 0.0 0.0 0.0 0.0 7.114240112006596E-5 9 0.0 0.0 0.0 3.557120056003298E-5 7.114240112006596E-5 10-11 0.0 0.0 0.0 3.557120056003298E-5 7.114240112006596E-5 12-13 0.0 0.0 0.0 3.557120056003298E-5 1.2449920196011544E-4 14-15 0.0 0.0 0.0 3.557120056003298E-5 1.600704025201484E-4 16-17 0.0 0.0 0.0 3.557120056003298E-5 1.778560028001649E-4 18-19 0.0 0.0 0.0 3.557120056003298E-5 2.1342720336019788E-4 20-21 0.0 0.0 0.0 5.335680084004947E-5 2.4899840392023087E-4 22-23 0.0 0.0 0.0 8.892800140008244E-5 2.6678400420024735E-4 24-25 0.0 0.0 0.0 1.956416030801814E-4 2.8456960448026384E-4 26-27 0.0 3.557120056003298E-5 0.0 6.047104095205606E-4 2.8456960448026384E-4 28-29 0.0 3.557120056003298E-5 0.0 0.0015651328246414512 2.8456960448026384E-4 30-31 0.0 3.557120056003298E-5 0.0 0.004766540875044419 2.8456960448026384E-4 32-33 0.0 3.557120056003298E-5 0.0 0.013107987406372154 2.8456960448026384E-4 34-35 0.0 3.557120056003298E-5 0.0 0.026393830815544472 3.2014080504029686E-4 36-37 0.0 3.557120056003298E-5 0.0 0.04761205194960415 3.2014080504029686E-4 38-39 0.0 3.557120056003298E-5 0.0 0.07911035004551335 3.2014080504029686E-4 40-41 0.0 3.557120056003298E-5 0.0 0.11233385136858415 3.2014080504029686E-4 42-43 0.0 3.557120056003298E-5 0.0 0.14820740713337743 3.2014080504029686E-4 44-45 0.0 3.557120056003298E-5 0.0 0.1947878942667406 3.3792640532031334E-4 46-47 0.0 3.557120056003298E-5 0.0 0.2510081767518727 3.734976058803463E-4 48-49 0.0 3.557120056003298E-5 0.0 0.3115503601050489 3.912832061603628E-4 50-51 0.0 3.557120056003298E-5 0.0 0.37741043794194995 4.2685440672039576E-4 52-53 0.0 3.557120056003298E-5 0.0 0.4429503749738107 4.2685440672039576E-4 54-55 0.0 3.557120056003298E-5 0.0 0.5183435345608006 4.2685440672039576E-4 56-57 0.0 3.557120056003298E-5 0.0 0.6092813087925248 4.2685440672039576E-4 58-59 0.0 5.335680084004947E-5 0.0 0.6966441773679659 4.4464000700041224E-4 60-61 0.0 7.114240112006596E-5 0.0 0.7851275387610479 4.624256072804288E-4 62-63 0.0 7.114240112006596E-5 0.0 0.8721702665314487 4.624256072804288E-4 64-65 1.778560028001649E-5 7.114240112006596E-5 0.0 0.9673587992300969 4.624256072804288E-4 66-67 3.557120056003298E-5 7.114240112006596E-5 0.0 1.074285828113556 4.624256072804288E-4 68-69 3.557120056003298E-5 7.114240112006596E-5 0.0 1.1955124796221486 4.624256072804288E-4 70-71 3.557120056003298E-5 7.114240112006596E-5 0.0 1.342972891543765 5.157824081204782E-4 72-73 3.557120056003298E-5 7.114240112006596E-5 0.0 1.5072051245294376 6.758528106406267E-4 74-75 3.557120056003298E-5 7.114240112006596E-5 0.0 1.6823043592861997 7.114240112006596E-4 76-77 3.557120056003298E-5 7.114240112006596E-5 0.0 1.890555952964913 7.114240112006596E-4 78-79 3.557120056003298E-5 7.114240112006596E-5 0.0 2.1328847567801374 7.114240112006596E-4 80-81 3.557120056003298E-5 7.114240112006596E-5 0.0 2.3991885497728243 7.114240112006596E-4 82-83 3.557120056003298E-5 7.114240112006596E-5 0.0 2.676643914141082 8.003520126007421E-4 84-85 3.557120056003298E-5 7.114240112006596E-5 0.0 2.950880084858656 8.181376128807585E-4 86-87 3.557120056003298E-5 7.114240112006596E-5 0.0 3.2390423805954836 8.181376128807585E-4 88-89 3.557120056003298E-5 7.114240112006596E-5 0.0 3.5613530288699424 8.181376128807585E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10225 0.0 29.66647 1 GTATCAA 14080 0.0 25.926987 1 ATCAACG 16770 0.0 21.561558 3 TATCAAC 16985 0.0 21.403427 2 TCAACGC 16970 0.0 21.310856 4 GTACATG 10535 0.0 21.223389 1 CAACGCA 17125 0.0 21.152681 5 AACGCAG 17400 0.0 20.70938 6 TACATGG 10790 0.0 20.276806 2 GAGTACT 8845 0.0 20.125433 12-13 GTATAGG 780 0.0 20.083883 1 ACATGGG 10865 0.0 19.216803 3 AGAGTAC 14815 0.0 19.088715 10-11 GTACTTT 9255 0.0 18.952148 14-15 ACGCAGA 19280 0.0 18.591629 7 CGCAGAG 19530 0.0 18.37792 8 AGTACTT 9600 0.0 17.925386 12-13 TCGCGGA 225 0.0 17.909565 82-83 GTACCGT 265 4.0884515E-7 17.891914 6 GCAGAGT 20160 0.0 17.26268 9 >>END_MODULE