FastQCFastQC Report
Fri 27 May 2016
SRR939445_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939445_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2693806
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103310.38350942866709775No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79460.29497298617643586No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT73140.2715117569713632No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50850.18876637738575086No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT46400.17224699922711584No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT33530.12447073026045678No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC27280.10126935644214914No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG135050.024.005771
TACATGG138250.023.0625612
GAGTACT113800.022.87297212-13
GTATCAA279050.022.5376821
AGTACTT118550.021.4111312-13
ACATGGG143050.021.3596443
GTACTTT123550.021.07096914-15
AGAGTAC201550.020.23142210-11
TATCAAC319850.019.6167552
CATGGGG45150.019.3973674
TCAACGC326950.019.2922784
CAACGCA326650.019.1071685
ATCAACG326650.019.103943
CATTCCG4900.019.0026449
AACGCAG330600.018.891516
ACTTTTT153150.018.73765616-17
CATGGGA97600.018.3878484
GGTATCA216650.018.0076831
CGCTCCG4600.017.56339848-49
TACTTTT145300.017.47667114-15
Produced by FastQC (version 0.11.5)