FastQCFastQC Report
Fri 27 May 2016
SRR939445_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939445_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2693806
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138830.5153674763513038No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123240.45749396949891713No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT110070.4086040345889793No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81970.3042906579018682No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT67820.2517627475772197No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT47490.1762933188210287No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC41760.15502229930440425No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36610.13590436727811878No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT32330.1200160664873417No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT31620.11738039042158196No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC29830.11073551696001865No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC27220.10104662325349338No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG154150.023.087761
TACATGG157000.022.0612742
CATTCCG4750.021.9420629
GAGTACT124100.021.55086512-13
ACATGGG158400.020.8162483
GTACTTT131450.020.38189714-15
AGTACTT127200.020.1131112-13
GTATCAA288900.019.3561921
GGTATCA229800.019.3436411
CATGGGA104750.018.588154
AGAGTAC224600.018.45638710-11
GTACCGT2854.409594E-818.2850536
CATGGGG52050.018.1125974
ACTTTTT154450.017.76082816-17
GTATAGG9300.017.3253271
TCAACGC333150.016.7088454
TATCAAC335750.016.6948152
CAACGCA334000.016.6521385
TACTTTT153100.016.633214-15
ATCAACG334400.016.6322213
Produced by FastQC (version 0.11.5)