##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939445_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2693806 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.975994188148665 31.0 31.0 31.0 30.0 34.0 2 31.22538445604472 31.0 31.0 34.0 30.0 34.0 3 31.21352354252682 31.0 31.0 34.0 28.0 34.0 4 35.15154877522732 35.0 35.0 37.0 33.0 37.0 5 34.77667248495252 35.0 35.0 37.0 32.0 37.0 6 34.74397005575012 35.0 35.0 37.0 32.0 37.0 7 34.612874498015074 35.0 35.0 37.0 30.0 37.0 8 34.63091662874015 35.0 35.0 37.0 30.0 37.0 9 35.96448519306884 37.0 35.0 39.0 30.0 39.0 10-11 35.82381730532934 37.0 35.0 39.0 30.0 39.0 12-13 35.794743942214104 37.0 35.0 39.0 30.0 39.0 14-15 36.62447351442532 38.0 35.5 40.0 30.0 41.0 16-17 36.64529052945906 38.0 35.5 40.0 30.5 41.0 18-19 36.63217210148021 38.0 35.0 40.0 30.0 41.0 20-21 36.54195829246798 38.0 35.0 40.0 30.0 41.0 22-23 36.40295459286972 38.0 35.0 40.0 30.0 41.0 24-25 36.18641691346741 38.0 34.0 40.0 29.5 41.0 26-27 35.82372728399892 38.0 34.0 40.0 28.0 41.0 28-29 35.55921473187007 38.0 34.0 40.0 27.0 41.0 30-31 35.342852083631854 38.0 34.0 40.0 27.0 41.0 32-33 35.127186590274135 37.5 33.0 40.0 27.0 41.0 34-35 35.0800380576775 38.0 33.0 40.0 26.5 41.0 36-37 35.0658074486433 38.0 33.0 40.0 26.5 41.0 38-39 35.03987183932325 38.0 33.0 40.0 26.0 41.0 40-41 34.846965965626325 38.0 33.0 40.0 25.5 41.0 42-43 34.53398184576024 38.0 33.0 40.0 24.5 41.0 44-45 34.32460782253807 37.5 33.0 40.0 24.0 41.0 46-47 33.980895988798004 37.0 33.0 40.0 23.5 41.0 48-49 33.64244771152785 37.0 32.5 40.0 23.0 41.0 50-51 33.381851180077554 37.0 32.0 40.0 22.0 41.0 52-53 33.03673705530391 37.0 31.0 40.0 19.5 41.0 54-55 32.83303122051105 36.5 31.0 39.5 19.5 41.0 56-57 32.443049722214596 36.0 31.0 39.0 18.0 40.0 58-59 32.01727834149898 36.0 30.0 39.0 15.0 40.0 60-61 31.712213128933563 35.0 30.0 39.0 13.5 40.0 62-63 31.366976500906155 35.0 29.0 38.5 12.5 40.0 64-65 30.945435009054105 35.0 29.0 38.0 8.0 40.0 66-67 29.21766044028412 33.5 25.5 37.5 5.0 39.5 68-69 25.504818646925578 30.0 16.0 35.5 2.0 39.0 70-71 24.879472946455685 29.5 15.0 35.0 2.0 38.0 72-73 26.74530571243809 31.0 21.5 35.0 2.0 38.0 74-75 27.377446074438915 32.0 24.5 35.0 2.0 38.0 76-77 27.43251295750325 31.5 25.5 34.5 2.0 36.5 78-79 28.259041296960508 33.0 26.0 35.0 2.0 37.0 80-81 28.09825967423044 33.0 26.0 35.0 2.0 37.0 82-83 27.684942790980493 33.0 26.0 35.0 2.0 36.0 84-85 27.335075911182912 32.5 26.0 35.0 2.0 36.0 86-87 26.906146173852164 32.0 25.5 35.0 2.0 35.5 88-89 26.4108360067503 32.0 25.0 34.0 2.0 35.0 90-91 25.957700183309413 32.0 24.0 34.0 2.0 35.0 92-93 25.393286673205125 31.5 21.5 34.0 2.0 35.0 94-95 24.007569216194483 31.0 12.0 34.0 2.0 35.0 96-97 23.198054722574675 30.0 4.5 34.0 2.0 35.0 98-99 22.888668486149335 30.0 2.0 34.0 2.0 35.0 100-101 21.635703907408327 30.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.40735750833791684 1101 2 0.3021459594704865 1101 3 0.32552825010526476 1101 4 0.35121488360459097 1101 5 0.4242101450299529 1101 6 0.549123084799426 1101 7 0.5882984382244771 1101 8 0.48159078108553643 1101 9 0.6795060511015834 1101 10-11 0.8014779019709977 1101 12-13 0.7050240049314382 1101 14-15 0.894829884812971 1101 16-17 0.8915967892405092 1101 18-19 0.8532341225624833 1101 20-21 0.8275342196187481 1101 22-23 0.9082698062913153 1101 24-25 0.8067329527628857 1101 26-27 0.9063203921669185 1101 28-29 0.9038006771812448 1101 30-31 1.018901598391274 1101 32-33 0.9456819924361852 1101 34-35 1.0260177439065217 1101 36-37 0.9820158806482908 1101 38-39 1.0288798525972211 1101 40-41 1.1921597280744791 1101 42-43 1.1945628611983707 1101 44-45 0.839330865170318 1101 46-47 0.8684392796709233 1101 48-49 0.8703096897116112 1101 50-51 0.8724591914789528 1101 52-53 0.972455976551494 1101 54-55 0.9158137796300778 1101 56-57 0.8686763306323826 1101 58-59 0.9335467449744925 1101 60-61 0.8307164648579111 1101 62-63 0.7673434726917066 1101 64-65 0.5398296439145476 1101 66-67 -2.6023186865604195 1101 68-69 -8.075785585778473 1101 70-71 -11.03527767918469 1101 72-73 -11.010783153753334 1101 74-75 -9.526024645446615 1101 76-77 -4.362805128242957 1101 78-79 -1.196334523208293 1101 80-81 -0.471988455962709 1101 82-83 -0.11763629265157505 1101 84-85 -0.23549117433096 1101 86-87 -0.1428741409910863 1101 88-89 -0.18092510281463348 1101 90-91 -0.17731345229936935 1101 92-93 -0.8375873585114313 1101 94-95 -4.790042308927671 1101 96-97 -1.5470195565006435 1101 98-99 -0.5813791832463195 1101 100-101 -0.4621348372781 1102 1 0.45192414519669555 1102 2 0.2673975628188785 1102 3 0.4392800426355272 1102 4 0.2656012254421398 1102 5 0.4799916796835504 1102 6 0.5352516336677127 1102 7 0.4819035998575387 1102 8 0.6002410175415207 1102 9 0.7620740092441309 1102 10-11 0.7147580850204776 1102 12-13 0.7406556951582957 1102 14-15 0.6751977923077348 1102 16-17 0.6631403553059698 1102 18-19 0.672733371386613 1102 20-21 0.8465757700218361 1102 22-23 0.8546446460418835 1102 24-25 0.7476544542434596 1102 26-27 0.8303695824428843 1102 28-29 0.7745689071819939 1102 30-31 0.48833143070694973 1102 32-33 0.4271872224821891 1102 34-35 0.6015923785036605 1102 36-37 0.8727098087216021 1102 38-39 0.709353646424006 1102 40-41 0.7760935430315499 1102 42-43 0.9419805606784806 1102 44-45 0.7013689710113837 1102 46-47 0.6114444080797199 1102 48-49 0.5738998641261901 1102 50-51 0.5088748547984281 1102 52-53 0.47523482550577967 1102 54-55 0.43745791529099165 1102 56-57 0.3991775835886102 1102 58-59 0.5350584597309407 1102 60-61 0.39378310299783337 1102 62-63 0.4984752500361722 1102 64-65 0.43463208551371935 1102 66-67 -2.6490716445022606 1102 68-69 -7.757548261177398 1102 70-71 -10.907817781043967 1102 72-73 -9.377245734604031 1102 74-75 -7.811957724174459 1102 76-77 -4.097672233316192 1102 78-79 -1.3642212634808004 1102 80-81 -0.5936949618546734 1102 82-83 -0.3925083706884571 1102 84-85 -0.4118945685868347 1102 86-87 -0.47364887491938035 1102 88-89 -0.48000263834281753 1102 90-91 -0.45779730308569455 1102 92-93 -0.9176953117514763 1102 94-95 -4.252765473073477 1102 96-97 -1.2341075627970248 1102 98-99 -0.7422815145819257 1102 100-101 -0.48250706648966 1103 1 0.3133317009437775 1103 2 0.20203130934392988 1103 3 0.294029001206507 1103 4 0.19469300639138254 1103 5 0.3296739698979181 1103 6 0.48728442706340047 1103 7 0.4809570190401118 1103 8 0.44885674079601756 1103 9 0.6381103684251883 1103 10-11 0.7519673087439287 1103 12-13 0.5926241649969839 1103 14-15 0.6921049593673061 1103 16-17 0.6891378457366528 1103 18-19 0.5894665796639629 1103 20-21 0.6869807866255471 1103 22-23 0.7080549564004102 1103 24-25 0.7836379392494166 1103 26-27 0.7490833014218197 1103 28-29 0.8589378603451436 1103 30-31 0.8119894026031531 1103 32-33 0.7914959924552321 1103 34-35 0.6974695448027646 1103 36-37 0.763978176318588 1103 38-39 0.8540331693523342 1103 40-41 0.8532191778173868 1103 42-43 1.0376233526377305 1103 44-45 0.7648924443567111 1103 46-47 0.904765425933995 1103 48-49 0.83399021284648 1103 50-51 0.678828148925021 1103 52-53 0.7929356332998125 1103 54-55 0.821568834874931 1103 56-57 0.8510638798191934 1103 58-59 0.8491525009825125 1103 60-61 0.7923574225834606 1103 62-63 0.6307367353294211 1103 64-65 0.601654168878742 1103 66-67 -2.4479629345222875 1103 68-69 -7.3536814542452404 1103 70-71 -11.458591353673608 1103 72-73 -8.978918358329178 1103 74-75 -7.12934251212852 1103 76-77 -3.37789448229589 1103 78-79 -0.6457041063074058 1103 80-81 -0.048502012308006215 1103 82-83 0.17617482364684278 1103 84-85 0.1768065602968747 1103 86-87 0.2564187440316701 1103 88-89 0.39864827803021896 1103 90-91 0.36811959806093597 1103 92-93 0.41248775161303186 1103 94-95 1.2603106927777894 1103 96-97 1.425364054565474 1103 98-99 1.1027790910946038 1103 100-101 1.0049073800108843 1104 1 0.35886917931891205 1104 2 0.2521965879226258 1104 3 0.35477904138385696 1104 4 0.21574467603589653 1104 5 0.410019526598397 1104 6 0.48675692212395205 1104 7 0.46969822335216094 1104 8 0.4028623435665253 1104 9 0.6166191113311257 1104 10-11 0.7350324779772208 1104 12-13 0.6200892422162738 1104 14-15 0.7948222085267318 1104 16-17 0.7435107167311372 1104 18-19 0.7176425595973512 1104 20-21 0.7794394197134054 1104 22-23 0.625250388472331 1104 24-25 0.7815372154269227 1104 26-27 0.9769320329205442 1104 28-29 1.0578236085734076 1104 30-31 1.1105226930806467 1104 32-33 0.7842533559389082 1104 34-35 0.7721092879198324 1104 36-37 0.9862384751120032 1104 38-39 1.0144745529656518 1104 40-41 1.095375614228466 1104 42-43 1.1533706670577715 1104 44-45 0.9526908202465236 1104 46-47 0.9096886478106398 1104 48-49 0.9413073744035358 1104 50-51 0.8937167710153133 1104 52-53 0.9958178459214864 1104 54-55 0.8232011380925783 1104 56-57 0.819153454821631 1104 58-59 0.8453455791345448 1104 60-61 0.746498367869787 1104 62-63 0.9811194442595053 1104 64-65 0.48368796196697517 1104 66-67 -2.534832504671936 1104 68-69 -7.139034999147757 1104 70-71 -8.446118729415314 1104 72-73 -7.92202219235104 1104 74-75 -6.799283553840485 1104 76-77 -3.4202395159030594 1104 78-79 -0.5959366622484517 1104 80-81 0.17410003027683274 1104 82-83 0.461721377375774 1104 84-85 0.4506496890250915 1104 86-87 0.406490590582564 1104 88-89 0.3413095309962557 1104 90-91 0.38360552338463805 1104 92-93 0.5990140023890085 1104 94-95 1.373665643099681 1104 96-97 1.681560977138215 1104 98-99 1.3967534343131405 1104 100-101 1.2333679100997799 1105 1 0.5448897646252959 1105 2 0.3671780431955689 1105 3 0.4518305595246801 1105 4 0.3021885422382127 1105 5 0.504411740663123 1105 6 0.62549036767426 1105 7 0.6868272214009181 1105 8 0.5586961161114772 1105 9 0.7669456167847954 1105 10-11 0.880163896900882 1105 12-13 0.8842564757105364 1105 14-15 0.9359862065308207 1105 16-17 0.979944129058751 1105 18-19 1.0351884152049848 1105 20-21 1.0687476369481104 1105 22-23 0.9641131621713583 1105 24-25 1.056502780384946 1105 26-27 1.1640233693702555 1105 28-29 1.2746268668642955 1105 30-31 1.2337412805165187 1105 32-33 1.2542543513434552 1105 34-35 1.2091621268859285 1105 36-37 1.1460333409514618 1105 38-39 1.039254383517168 1105 40-41 1.2247675709343255 1105 42-43 1.324901788551152 1105 44-45 1.07774952359172 1105 46-47 1.0617626944236491 1105 48-49 0.9826715380531468 1105 50-51 1.1549723930304339 1105 52-53 1.236143864307543 1105 54-55 1.23478081958865 1105 56-57 1.3576166416560795 1105 58-59 1.3750296381560787 1105 60-61 1.3849347340816784 1105 62-63 1.472083276618065 1105 64-65 1.4705835567913788 1105 66-67 2.185373473880926 1105 68-69 -4.543342963277464 1105 70-71 -5.101238437446995 1105 72-73 -1.3796650701251458 1105 74-75 1.3353306609618585 1105 76-77 1.508785587433973 1105 78-79 1.4920442851129394 1105 80-81 1.4624125770959395 1105 82-83 1.7704015390056504 1105 84-85 1.6205048938210709 1105 86-87 1.7965786264579648 1105 88-89 1.726717091731885 1105 90-91 1.7220973600038718 1105 92-93 1.7290787079385588 1105 94-95 2.523120903706374 1105 96-97 2.6207012942979233 1105 98-99 2.2785264391119284 1105 100-101 2.224188068147903 1106 1 0.12147684480023457 1106 2 0.1973895711281557 1106 3 0.20680329967034083 1106 4 0.11052608966583222 1106 5 0.2890963500067798 1106 6 0.3614528602750937 1106 7 0.2831520520640183 1106 8 0.22440388576886505 1106 9 0.47096810589239624 1106 10-11 0.34848245585922655 1106 12-13 0.233062558554181 1106 14-15 0.5343271600790942 1106 16-17 0.35387873633347056 1106 18-19 0.3676958078370518 1106 20-21 0.5302129025754354 1106 22-23 0.45794267189469906 1106 24-25 0.3880743065230021 1106 26-27 0.44799239937069046 1106 28-29 0.6438839928215074 1106 30-31 0.5097393566245856 1106 32-33 0.5655634341431153 1106 34-35 0.7061719166516198 1106 36-37 0.7625012156149324 1106 38-39 0.5366118088384084 1106 40-41 0.4903929316368405 1106 42-43 0.6084300264673672 1106 44-45 0.5734538152601871 1106 46-47 0.48552847658785936 1106 48-49 0.5178710944734419 1106 50-51 0.39472931274747225 1106 52-53 0.6981270170772831 1106 54-55 0.7211242140448562 1106 56-57 0.6487228446722 1106 58-59 0.7632229111113489 1106 60-61 0.6567224182114622 1106 62-63 0.8029740686369422 1106 64-65 0.7588210178463974 1106 66-67 1.2913835890139005 1106 68-69 -5.382617461756688 1106 70-71 -6.311082746808122 1106 72-73 -2.3821507324043623 1106 74-75 0.33200838663150734 1106 76-77 0.6497939733727058 1106 78-79 0.5129869725595562 1106 80-81 0.5812859700233162 1106 82-83 0.6583683724502727 1106 84-85 0.7281179981515926 1106 86-87 0.7552031351947477 1106 88-89 0.6873002831798196 1106 90-91 0.6582190408329431 1106 92-93 0.7106553846362935 1106 94-95 1.575272280506514 1106 96-97 1.6623479249607627 1106 98-99 1.1584834989067687 1106 100-101 0.969673760481129 1107 1 -0.04414866468433942 1107 2 -0.08716437758450013 1107 3 -0.07958311302620658 1107 4 -0.14984614752501102 1107 5 -0.03252363747677123 1107 6 0.036967625543908866 1107 7 0.2111560642532524 1107 8 0.15115317737524947 1107 9 0.10605234010553488 1107 10-11 0.015975804860332232 1107 12-13 0.06566508237342106 1107 14-15 0.0528876937677083 1107 16-17 -0.1691470326628206 1107 18-19 0.04215631387464214 1107 20-21 -0.014552242054669762 1107 22-23 0.1995450274380559 1107 24-25 0.2931334975933737 1107 26-27 0.25094350491589523 1107 28-29 -0.016897689101071478 1107 30-31 -0.04526025430152458 1107 32-33 -0.052994900679856016 1107 34-35 0.08456638699445307 1107 36-37 0.08101351097658949 1107 38-39 -0.007779072910125251 1107 40-41 0.04510310120812022 1107 42-43 0.21264423790687204 1107 44-45 0.14402914218764096 1107 46-47 0.049325714744263394 1107 48-49 0.13873413670317092 1107 50-51 0.07761796823000111 1107 52-53 0.26169565295914765 1107 54-55 0.1618361885088504 1107 56-57 0.3115571050039918 1107 58-59 0.04765785903867936 1107 60-61 -0.006350242448998955 1107 62-63 0.2545716812385166 1107 64-65 0.21004094964055042 1107 66-67 1.032930289331187 1107 68-69 -5.626894002492154 1107 70-71 -6.007078350444019 1107 72-73 -2.3819429225121347 1107 74-75 0.002250659470174554 1107 76-77 0.03474584840695627 1107 78-79 0.14000399029847443 1107 80-81 -0.09208626842075773 1107 82-83 0.14347476051952412 1107 84-85 0.18206110540850062 1107 86-87 0.44690155154437505 1107 88-89 0.33911993563828347 1107 90-91 0.12352658067485223 1107 92-93 0.3900490400316663 1107 94-95 1.163566021156349 1107 96-97 1.2501189508158923 1107 98-99 0.9477928648325289 1107 100-101 0.7351261178416806 1108 1 0.10403923826500261 1108 2 -0.024755150620578092 1108 3 0.02750718264507057 1108 4 -0.021291830230396158 1108 5 0.10047337484623853 1108 6 0.17223523090513737 1108 7 0.1943130582926429 1108 8 -0.07947346068638694 1108 9 0.20820967419822978 1108 10-11 0.22853584408164807 1108 12-13 0.18744454029712898 1108 14-15 0.21360250608408649 1108 16-17 0.14650422009724196 1108 18-19 0.2904172269131564 1108 20-21 0.2736599806959177 1108 22-23 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60-61 0.8590479295550857 2311 62-63 0.9709668576452728 2311 64-65 1.0011884077815303 2311 66-67 2.0688274603093753 2311 68-69 4.4924051144287205 2311 70-71 4.800686508400272 2311 72-73 2.820534944247143 2311 74-75 1.7043375014876112 2311 76-77 1.1525375764562789 2311 78-79 0.8013885835911871 2311 80-81 0.6566780332963873 2311 82-83 0.5648769333025889 2311 84-85 0.610275330704475 2311 86-87 0.8330230758452615 2311 88-89 0.817554243417721 2311 90-91 0.7521290303719717 2311 92-93 0.8178977505387124 2311 94-95 1.548194989440507 2311 96-97 2.0227229109068183 2311 98-99 1.6626280340939417 2311 100-101 1.1941294497356303 2312 1 -0.17480595628105533 2312 2 -0.1305513483813563 2312 3 -0.13434987311371316 2312 4 0.05833695260964333 2312 5 0.03358916023589131 2312 6 0.1499975355276888 2312 7 0.27364877495376305 2312 8 0.19329840496043715 2312 9 -1.6049450481163703 2312 10-11 -1.0573193826116238 2312 12-13 -1.7528576019265714 2312 14-15 -1.844772396920014 2312 16-17 0.09582386315233293 2312 18-19 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1.0575500627824432 2312 86-87 1.3576823813052172 2312 88-89 1.2816504800120079 2312 90-91 1.5145947590391486 2312 92-93 1.6135178453640755 2312 94-95 2.4437924930647164 2312 96-97 2.714317445951057 2312 98-99 2.4180536715503465 2312 100-101 2.2040441030616336 2313 1 -0.19878765111540275 2313 2 -0.16850473171576397 2313 3 -0.05096865013770113 2313 4 -0.04563528669780936 2313 5 -0.02425433702384794 2313 6 0.09366095549078324 2313 7 -3.6422727717133903 2313 8 -1.0933009102023732 2313 9 -0.7947624554659782 2313 10-11 0.03980127174756376 2313 12-13 -1.2458937899722216 2313 14-15 -0.38629432364427174 2313 16-17 -0.018477850776669413 2313 18-19 0.33851198796985216 2313 20-21 0.20751610723868197 2313 22-23 -1.2406695650069466 2313 24-25 -2.7399915552564593 2313 26-27 -2.139031923919461 2313 28-29 -0.4594137867397805 2313 30-31 -0.14666690692374829 2313 32-33 -2.522691761682289 2313 34-35 -2.719098132506339 2313 36-37 -1.2995580059486258 2313 38-39 -0.6149982911081153 2313 40-41 -0.5885598064682327 2313 42-43 -0.6149862488976865 2313 44-45 -0.2746773625924206 2313 46-47 -0.16497985297968398 2313 48-49 -0.3083278095565234 2313 50-51 -0.18700063839050785 2313 52-53 0.18638086639309392 2313 54-55 0.21748664056760703 2313 56-57 0.41363336689005337 2313 58-59 0.10764253144299474 2313 60-61 0.4358849306740744 2313 62-63 0.28325887465226174 2313 64-65 0.20274362816918412 2313 66-67 1.5826839976721665 2313 68-69 4.603472724200724 2313 70-71 4.643286706423165 2313 72-73 2.705050264782173 2313 74-75 1.7892501963759138 2313 76-77 1.160296822202291 2313 78-79 0.733981750173875 2313 80-81 0.8320430487426371 2313 82-83 0.4799483786471761 2313 84-85 0.6531998319023415 2313 86-87 0.8242394220007014 2313 88-89 0.9117771198115108 2313 90-91 0.9796285877931332 2313 92-93 1.0659283162125952 2313 94-95 1.6356758871398647 2313 96-97 1.799548004745155 2313 98-99 1.5694506567978905 2313 100-101 1.1234162285355538 2314 1 -0.20142031114616188 2314 2 -0.2917482381781795 2314 3 -0.136631322219241 2314 4 -0.14870798486429493 2314 5 -1.4840440342679386 2314 6 -2.7596804116959888 2314 7 -0.9085095223525173 2314 8 -0.25836490973912873 2314 9 0.05211968443888537 2314 10-11 0.08823886507499878 2314 12-13 -0.01772859078327116 2314 14-15 -2.5825444473209345 2314 16-17 -2.8451432453405516 2314 18-19 -0.4993773199378495 2314 20-21 -0.07887458151606808 2314 22-23 0.011807957757540066 2314 24-25 -2.21534023224676 2314 26-27 -1.225018777033533 2314 28-29 -0.05731246899632225 2314 30-31 -0.001626443568277125 2314 32-33 0.0519978839937707 2314 34-35 -0.022405280584123943 2314 36-37 0.40958811671399076 2314 38-39 -1.1771593201708725 2314 40-41 -3.8506844506556064 2314 42-43 -5.153104877269961 2314 44-45 -1.9085201297761856 2314 46-47 -2.6674582876458075 2314 48-49 -0.5302114882954001 2314 50-51 -0.4983814216137077 2314 52-53 -1.995533935548707 2314 54-55 -1.2077810823547281 2314 56-57 -3.6652821449671755 2314 58-59 -4.962306593564584 2314 60-61 -2.409993205526643 2314 62-63 -1.476954195725117 2314 64-65 -0.40679810022182394 2314 66-67 -0.46409370669752903 2314 68-69 1.6641459857400456 2314 70-71 1.7181061840950242 2314 72-73 -0.38852816947359514 2314 74-75 0.21175930285365396 2314 76-77 0.2070597081703589 2314 78-79 0.32645746737311754 2314 80-81 0.5804760597643366 2314 82-83 0.42202313269590874 2314 84-85 0.3064955128170155 2314 86-87 0.06385092454559071 2314 88-89 0.2664759665751042 2314 90-91 0.8855115616320397 2314 92-93 0.939542966641941 2314 94-95 0.9128899957172543 2314 96-97 1.0997778267556342 2314 98-99 0.6158438806391899 2314 100-101 0.7230559589956691 2315 1 -0.3740703525807838 2315 2 -0.34688018669289633 2315 3 -0.35726606361281554 2315 4 -0.25496165557763106 2315 5 -1.2991052063509798 2315 6 -0.44667991758735326 2315 7 -0.2374514427801202 2315 8 -0.20777643053620665 2315 9 -0.008540664567910028 2315 10-11 -1.9002737066140725 2315 12-13 -1.4300980956171045 2315 14-15 -0.22535528616444367 2315 16-17 -1.787369727532024 2315 18-19 -3.2686672732813236 2315 20-21 -2.185681809243988 2315 22-23 -1.6548273416740855 2315 24-25 -1.2905177857338685 2315 26-27 -0.5730564726829286 2315 28-29 -0.6777937200530957 2315 30-31 -3.076883311349711 2315 32-33 -2.644867214789123 2315 34-35 -1.7234816547229386 2315 36-37 -4.711371409993262 2315 38-39 -2.3098014988852498 2315 40-41 -1.841772571077108 2315 42-43 -1.7102927239899302 2315 44-45 -1.4726317708441954 2315 46-47 -1.3443411133975331 2315 48-49 -4.566412712991752 2315 50-51 -4.16128865047682 2315 52-53 -2.7242293095088854 2315 54-55 -4.401912451919905 2315 56-57 -6.700851245193558 2315 58-59 -7.261555243727905 2315 60-61 -6.266273762441447 2315 62-63 -6.875902855627217 2315 64-65 -6.499184913794693 2315 66-67 -4.7046542600992005 2315 68-69 -2.5389723953999415 2315 70-71 0.1498062703101084 2315 72-73 -1.692694305884622 2315 74-75 -3.0650399665586328 2315 76-77 -5.527343905096128 2315 78-79 -4.498978492383614 2315 80-81 -4.285921625393463 2315 82-83 -7.563384978930824 2315 84-85 -5.238116611903891 2315 86-87 -5.602521338215048 2315 88-89 -4.028007701849919 2315 90-91 -3.927556596083612 2315 92-93 -5.04904906870923 2315 94-95 -3.167971151391491 2315 96-97 -1.7655421698268334 2315 98-99 -3.3178877568320466 2315 100-101 -2.4279420269291023 2316 1 -0.41470913481523297 2316 2 -0.36103571168263215 2316 3 -0.8950376702813827 2316 4 -0.43967990315644556 2316 5 -0.34909336390548873 2316 6 -2.466211260955255 2316 7 -0.40159135891966713 2316 8 -0.3259017460631384 2316 9 -0.17603811319089147 2316 10-11 -0.07451175208181837 2316 12-13 -0.6246089953749632 2316 14-15 -1.1390311015729537 2316 16-17 -1.4570967790817946 2316 18-19 -0.18640229512284634 2316 20-21 -3.9470003985516016 2316 22-23 -4.3989367209633485 2316 24-25 -2.699573055549692 2316 26-27 -2.2489598846834156 2316 28-29 -1.4357804898323323 2316 30-31 -0.2931075341122664 2316 32-33 -2.221969683450098 2316 34-35 -2.2005712306909615 2316 36-37 -4.28930085799044 2316 38-39 -1.9459436285118628 2316 40-41 -1.489134965119817 2316 42-43 -2.8748708468283226 2316 44-45 -0.5809278566081346 2316 46-47 -0.693094252369896 2316 48-49 -0.24410245106003714 2316 50-51 0.03366353429140645 2316 52-53 -2.412425868029036 2316 54-55 -0.7319467406887057 2316 56-57 0.23720135699370815 2316 58-59 0.4813769530203871 2316 60-61 -0.9337612518212701 2316 62-63 -0.41253675457312866 2316 64-65 0.5819428998725762 2316 66-67 1.817698808470368 2316 68-69 4.15668146331787 2316 70-71 4.301408766856969 2316 72-73 2.4921066939509586 2316 74-75 1.3742667759576328 2316 76-77 0.23988558556282769 2316 78-79 0.8004811009750554 2316 80-81 0.34423300724808215 2316 82-83 0.5516317851915638 2316 84-85 0.8194436487602879 2316 86-87 0.6136668501949991 2316 88-89 0.9313991473569949 2316 90-91 0.7380933223954571 2316 92-93 1.1228907455925317 2316 94-95 1.6664921033471138 2316 96-97 1.7401706331242934 2316 98-99 0.7380638144468001 2316 100-101 0.7505598421492188 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 28.0 4 69.0 5 172.0 6 410.0 7 1066.0 8 2395.0 9 3984.0 10 6665.0 11 9963.0 12 13400.0 13 17240.0 14 20531.0 15 26584.0 16 32341.0 17 36379.0 18 33356.0 19 27844.0 20 26918.0 21 27643.0 22 28904.0 23 32911.0 24 39684.0 25 49788.0 26 63038.0 27 78313.0 28 95974.0 29 114942.0 30 136220.0 31 157695.0 32 182958.0 33 211865.0 34 246577.0 35 283214.0 36 303024.0 37 261188.0 38 113203.0 39 7315.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.53663755732538 20.996339270430965 15.80663594955998 21.660387222683678 2 15.6419795716052 21.964942765593264 39.060078490779006 23.332999172022532 3 19.589086964688622 25.90828738223911 30.764873194283478 23.73775245878879 4 12.889309772121674 18.12287150596591 37.66841413227233 31.319404589640087 5 12.949508890968561 38.75059813252724 34.08477463628531 14.215118340218897 6 30.254814192261804 37.61774233185315 17.34026132542581 14.787182150459238 7 27.279061669622827 34.06566768356741 20.926562640368314 17.728708006441444 8 26.129684171762925 34.67554827630497 19.48915400737841 19.705613544553692 9 25.906950983107173 17.467441976148244 21.261182134125473 35.364424906619114 10-11 24.749666457049987 27.420905588598437 26.95765767839258 20.871770275958994 12-13 26.157321648255294 25.05557193056961 27.632910462000602 21.15419595917449 14-15 22.752696370859667 26.18265754846489 27.18278524882638 23.881860831849064 16-17 21.106647558545227 29.607577566728853 27.607609863191072 21.678165011534848 18-19 20.80289897897969 28.045093578679147 30.452439252558488 20.699568189782667 20-21 22.2902961220831 26.45251353615305 30.238738557308075 21.018451784455777 22-23 21.625035358893697 26.80289523447494 30.28198764127781 21.290081765353555 24-25 21.750489827404053 26.975309283593546 30.436880012888828 20.837320876113573 26-27 21.461498940528536 26.862371909379924 30.79795203497221 20.878177115119335 28-29 20.73253614515649 27.562936226245355 30.67154684988888 21.032980778709273 30-31 21.19883817063849 26.89373619687246 30.54934452197976 21.358081110509293 32-33 21.204628058673062 27.068667233448547 30.452206551157012 21.27449815672138 34-35 21.95361276950099 26.867995053355376 30.257192849715043 20.921199327428592 36-37 21.649911112696614 26.530927727784864 30.63579690886227 21.18336425065625 38-39 21.179798715517745 27.259192093549995 30.55800562437116 21.003003566561098 40-41 21.395072073192104 27.142474057943623 30.46629451414174 20.996159354722533 42-43 21.143464570967136 27.25001495742615 29.87832273183174 21.728197739774973 44-45 21.511634928400085 27.083298109192917 29.722126588035856 21.682940374371146 46-47 21.882498783472617 27.51364099259716 29.30351090080996 21.300349323120262 48-49 21.77283387421214 27.58342558559282 29.103404656500153 21.54033588369489 50-51 21.18777561298632 27.779317864013525 29.295660759548237 21.73724576345192 52-53 21.848025960279763 28.122373242737126 28.47873451623827 21.55086628074484 54-55 21.366381225361927 27.612192845987998 28.852714664194128 22.168711264455947 56-57 21.841514599421117 27.783463768643387 28.544332284354667 21.830689347580826 58-59 22.346077290958835 27.440046543730723 28.533504914455104 21.680371250855337 60-61 21.806521361014877 27.739294061565655 29.16097949797731 21.29320507944216 62-63 21.560481150365522 27.66403001018097 29.124631902893828 21.650856936559688 64-65 21.363023478040706 28.025505018068316 28.743968257386033 21.86750324650495 66-67 21.1597945783493 27.740181019953653 28.841333231178023 22.258691170519022 68-69 21.03417962480889 27.400914498626904 29.5674538656573 21.99745201090691 70-71 20.94816842382112 27.45435741272783 29.80242104606529 21.79505311738576 72-73 21.68110533680127 27.339856593022343 29.0439615484201 21.93507652175629 74-75 21.03471576127721 28.30704083709682 28.632737527058516 22.025505874567457 76-77 21.389868542254753 27.885233345201794 28.359334420677406 22.365563691866043 78-79 21.495404996625307 27.856070995530853 28.416013941307806 22.232510066536033 80-81 21.52426181972882 28.04599651157978 28.23181419197151 22.19792747671989 82-83 21.477233327121553 27.9488946123069 28.417246824752784 22.156625235818765 84-85 21.299158142791278 28.034182862463 28.194365147304595 22.47229384744113 86-87 21.295018670658056 28.004409386149447 28.312468747668255 22.388103195524238 88-89 21.308882010923284 28.040797575903813 27.795882191895238 22.85443822127766 90-91 21.28764825703665 27.840155443203574 28.17279988952069 22.699396410239085 92-93 21.625750786494038 27.99683045083663 28.15625454174901 22.221164220920322 94-95 21.011038026151713 27.687673698734976 28.47994177842576 22.82134649668755 96-97 20.61028453762148 27.711157635133464 28.770134182300616 22.90842364494444 98-99 21.071367112730847 27.878741976126125 27.780540656738495 23.269350254404532 100-101 20.811196007726455 27.488274998208716 28.256762410793275 23.443766583271554 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1555.0 1 1085.5 2 670.5 3 730.0 4 712.5 5 731.5 6 1143.5 7 2238.5 8 3415.0 9 4169.0 10 4195.5 11 3882.5 12 3879.5 13 4022.5 14 4278.5 15 4481.0 16 4669.5 17 4962.5 18 5297.0 19 5777.5 20 6394.0 21 7337.5 22 8535.0 23 10033.5 24 11585.0 25 13768.0 26 17416.5 27 21521.5 28 25160.5 29 29269.0 30 34236.5 31 39645.5 32 46675.5 33 54733.5 34 62668.5 35 69237.5 36 76723.5 37 83868.5 38 86089.0 39 86104.0 40 86229.5 41 87456.5 42 89366.5 43 91278.5 44 93575.5 45 95835.5 46 98408.5 47 106739.0 48 120223.0 49 117200.5 50 100989.0 51 90139.5 52 82602.0 53 77875.5 54 74238.5 55 68353.5 56 61426.5 57 55189.0 58 49434.0 59 43176.0 60 36371.5 61 29275.5 62 22931.5 63 17686.0 64 13185.5 65 9774.0 66 6998.5 67 5076.0 68 3419.0 69 2072.0 70 1397.5 71 1007.5 72 764.0 73 614.5 74 453.5 75 347.0 76 252.0 77 134.5 78 67.0 79 42.5 80 23.5 81 16.0 82 13.0 83 7.0 84 7.0 85 5.5 86 1.5 87 1.0 88 1.5 89 1.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02327561821452621 2 0.018412610262208935 3 0.0 4 0.0 5 1.8561099054646102E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 4.640274763661525E-4 18-19 0.001113665943278766 20-21 5.568329716393831E-5 22-23 0.0 24-25 0.0 26-27 3.7122198109292205E-4 28-29 0.01724326102176623 30-31 0.004566030367442941 32-33 0.00876083875379296 34-35 0.00976313810274385 36-37 0.1023830223854279 38-39 0.10537135933322593 40-41 0.12036872736937998 42-43 0.10524143163984341 44-45 0.10047122918279934 46-47 0.10188187271095246 48-49 0.10449898767765757 50-51 0.10242014458353721 52-53 0.08001689802457934 54-55 0.06808211133244191 56-57 0.03816161965635238 58-59 0.015776934196449186 60-61 0.00900213304150336 62-63 0.001986037598847133 64-65 0.005197107735300909 66-67 0.4311928921384836 68-69 0.82736099036085 70-71 0.9158231884552934 72-73 0.284894309389763 74-75 0.14973238607383013 76-77 0.014589023856951835 78-79 0.008463861168918622 80-81 0.0013549602309891656 82-83 0.0 84-85 0.0 86-87 2.598553867650454E-4 88-89 6.867606650219058E-4 90-91 0.003303875631727006 92-93 0.0017633044101913798 94-95 0.22802310188632735 96-97 0.23407402017814202 98-99 0.013345430220290546 100-101 0.007535806216186317 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2693806.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.14942934298865 #Duplication Level Percentage of deduplicated Percentage of total 1 71.42358803517665 32.96157829452114 2 14.798878174162564 13.659195653080241 3 5.511479991856967 7.630549693784962 4 2.761181027569094 5.097077149400028 5 1.4860630439098719 3.4290480722072627 6 0.9034753877235454 2.5016924141326213 7 0.620276452796098 2.003778303200321 8 0.42477863310431196 1.5682633211886992 9 0.30726299154669584 1.2762010546289604 >10 1.535674153161707 13.003315630719358 >50 0.13556956783680546 4.334099921271687 >100 0.08459817877374942 7.0875211126076305 >500 0.004647030179259641 1.428890874258201 >1k 0.002119697976501521 2.055069999546421 >5k 1.63053690500117E-4 0.5635725000129224 >10k+ 2.445805357501755E-4 1.4001460054396753 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13883 0.5153674763513038 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12324 0.45749396949891713 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 11007 0.4086040345889793 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8197 0.3042906579018682 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6782 0.2517627475772197 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4749 0.1762933188210287 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4176 0.15502229930440425 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3661 0.13590436727811878 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3233 0.1200160664873417 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3162 0.11738039042158196 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2983 0.11073551696001865 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2722 0.10104662325349338 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 5.56832971639383E-5 0.0 18-19 0.0 0.0 0.0 1.113665943278766E-4 0.0 20-21 0.0 0.0 0.0 1.4848879243716881E-4 0.0 22-23 0.0 0.0 0.0 2.412942877103993E-4 0.0 24-25 0.0 0.0 0.0 4.4546637731150647E-4 0.0 26-27 0.0 0.0 0.0 8.538105565137207E-4 0.0 28-29 0.0 0.0 0.0 0.0021345263912843017 0.0 30-31 0.0 0.0 0.0 0.006514945768180782 0.0 32-33 0.0 0.0 0.0 0.01718757772460229 0.0 34-35 0.0 0.0 0.0 0.03248192334563068 0.0 36-37 0.0 0.0 0.0 0.0576136514656215 0.0 38-39 0.0 0.0 0.0 0.09473584957491371 0.0 40-41 0.0 0.0 0.0 0.1368509833299057 0.0 42-43 0.0 0.0 0.0 0.18215862612229683 0.0 44-45 0.0 0.0 0.0 0.23778623998907122 0.0 46-47 0.0 0.0 0.0 0.30468044098201574 0.0 48-49 0.0 3.7122198109292203E-5 0.0 0.378200954337469 0.0 50-51 0.0 3.7122198109292203E-5 0.0 0.4549696600274853 0.0 52-53 0.0 3.7122198109292203E-5 0.0 0.5338357699106766 0.0 54-55 0.0 3.7122198109292203E-5 0.0 0.6221680403117373 0.0 56-57 0.0 3.7122198109292203E-5 0.0 0.7243468906075642 0.0 58-59 0.0 3.7122198109292203E-5 0.0 0.8211058999794343 0.0 60-61 0.0 3.7122198109292203E-5 0.0 0.9186630366106543 0.0 62-63 0.0 3.7122198109292203E-5 0.0 1.0202850539348416 0.0 64-65 1.8561099054646102E-5 3.7122198109292203E-5 0.0 1.1275496453716416 0.0 66-67 3.7122198109292203E-5 3.7122198109292203E-5 0.0 1.2373571073789278 0.0 68-69 3.7122198109292203E-5 3.7122198109292203E-5 0.0 1.3730201803693363 0.0 70-71 3.7122198109292203E-5 3.7122198109292203E-5 0.0 1.5518934919589606 0.0 72-73 3.7122198109292203E-5 3.7122198109292203E-5 0.0 1.7457827326837938 0.0 74-75 3.7122198109292203E-5 3.7122198109292203E-5 0.0 1.956785306737011 0.0 76-77 3.7122198109292203E-5 3.7122198109292203E-5 0.0 2.1907665214198797 0.0 78-79 3.7122198109292203E-5 3.7122198109292203E-5 0.0 2.4585660585803133 0.0 80-81 3.7122198109292203E-5 3.7122198109292203E-5 0.0 2.7577338531430993 0.0 82-83 3.7122198109292203E-5 3.7122198109292203E-5 0.0 3.058535024422694 0.0 84-85 3.7122198109292203E-5 3.7122198109292203E-5 0.0 3.3619161884708846 0.0 86-87 3.7122198109292203E-5 3.7122198109292203E-5 0.0 3.669529283103534 0.0 88-89 3.7122198109292203E-5 3.7122198109292203E-5 0.0 4.009624301081815 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 15415 0.0 23.08776 1 TACATGG 15700 0.0 22.061274 2 CATTCCG 475 0.0 21.942062 9 GAGTACT 12410 0.0 21.550865 12-13 ACATGGG 15840 0.0 20.816248 3 GTACTTT 13145 0.0 20.381897 14-15 AGTACTT 12720 0.0 20.11311 12-13 GTATCAA 28890 0.0 19.356192 1 GGTATCA 22980 0.0 19.343641 1 CATGGGA 10475 0.0 18.58815 4 AGAGTAC 22460 0.0 18.456387 10-11 GTACCGT 285 4.409594E-8 18.285053 6 CATGGGG 5205 0.0 18.112597 4 ACTTTTT 15445 0.0 17.760828 16-17 GTATAGG 930 0.0 17.325327 1 TCAACGC 33315 0.0 16.708845 4 TATCAAC 33575 0.0 16.694815 2 CAACGCA 33400 0.0 16.652138 5 TACTTTT 15310 0.0 16.6332 14-15 ATCAACG 33440 0.0 16.632221 3 >>END_MODULE