FastQCFastQC Report
Fri 27 May 2016
SRR939444_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939444_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2660031
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100910.3793564811838659No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72140.2711998469190773No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT65860.24759109950222385No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51600.19398270170535606No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT43080.16195299979586703No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT30240.11368288565058075No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC26830.10086348617741672No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG132750.025.3068121
TACATGG136350.023.973762
GAGTACT109100.022.5471512-13
ACATGGG141750.022.5200773
GTATCAA260100.022.4535621
AGTACTT114300.021.45833212-13
GTACTTT118250.020.70398514-15
AGAGTAC190250.020.08738110-11
CATGGGA97300.019.6934194
TATCAAC299500.019.218242
CATGGGG48650.019.0075784
GTATCGA3455.16593E-1018.9221341
ATCAACG309950.018.8300693
GGTATCA203950.018.6880471
ACTTTTT148650.018.57329616-17
TCAACGC311050.018.5576024
CAACGCA311250.018.4568315
AACGCAG316900.018.0363036
TACTTTT138600.017.85239414-15
ATGGGAG40150.017.2662725
Produced by FastQC (version 0.11.5)