##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939444_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2660031 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.931153809861616 31.0 26.0 31.0 10.0 33.0 2 26.976034865759082 31.0 26.0 31.0 16.0 34.0 3 27.24889221215843 31.0 26.0 31.0 16.0 34.0 4 31.064384212063693 35.0 30.0 37.0 19.0 37.0 5 31.485391335664886 35.0 30.0 37.0 19.0 37.0 6 31.309502408054644 35.0 30.0 37.0 17.0 37.0 7 31.280906500713712 35.0 30.0 37.0 17.0 37.0 8 31.20717126980851 35.0 30.0 37.0 17.0 37.0 9 32.2457997669952 35.0 30.0 39.0 17.0 39.0 10-11 31.37074812286022 35.0 29.5 38.5 14.0 39.0 12-13 31.1162939454465 35.0 28.5 38.0 13.0 39.0 14-15 31.885219194813896 36.0 29.0 39.0 16.5 40.0 16-17 31.81092645160902 36.0 28.0 39.0 16.5 40.0 18-19 31.785022994092927 36.0 28.5 39.0 16.0 40.0 20-21 31.697444127530844 36.0 27.5 39.0 13.5 40.0 22-23 31.877338647557117 36.0 29.5 39.0 15.0 40.0 24-25 31.935067110120144 36.0 29.5 39.0 13.5 41.0 26-27 32.031950755461125 36.0 30.0 39.0 15.5 41.0 28-29 31.642599465946073 36.0 29.0 39.0 10.5 40.0 30-31 31.361661010717544 36.0 28.5 39.0 10.0 40.0 32-33 31.21237853994935 35.0 29.0 39.0 9.5 40.0 34-35 30.75143503966683 35.0 27.0 39.0 9.0 40.0 36-37 30.368813183004256 35.0 26.5 38.0 9.0 40.0 38-39 29.99594873142456 35.0 25.0 38.0 8.5 40.0 40-41 29.86773744366137 35.0 25.5 38.0 8.0 40.0 42-43 29.580920861448604 34.5 25.0 38.0 7.5 40.0 44-45 29.215003509357597 34.0 24.5 38.0 2.0 40.0 46-47 28.609566580239104 34.0 24.0 38.0 2.0 40.0 48-49 28.252910210444917 33.0 23.0 38.0 2.0 40.0 50-51 27.60176046820507 33.0 22.5 37.5 2.0 39.5 52-53 27.736944607036534 33.0 23.0 38.0 2.0 39.5 54-55 27.86790304323521 34.0 23.0 38.0 2.0 40.0 56-57 27.416559243106565 33.0 21.5 38.0 2.0 40.0 58-59 27.025104782613436 33.0 20.0 38.0 2.0 40.0 60-61 26.40438588873588 32.5 17.0 37.5 2.0 39.5 62-63 25.861656311524186 31.5 14.0 37.0 2.0 39.0 64-65 25.343913285221113 31.0 13.0 36.0 2.0 39.0 66-67 24.943047280276055 31.0 10.0 36.0 2.0 39.0 68-69 24.24048573118133 30.5 8.0 35.5 2.0 38.5 70-71 23.61857512186888 30.0 6.5 35.0 2.0 38.0 72-73 23.127308290768042 29.0 2.0 34.5 2.0 37.5 74-75 22.396334478808704 29.0 2.0 34.0 2.0 37.0 76-77 21.613386272565997 28.0 2.0 34.0 2.0 36.0 78-79 21.084565743782683 27.5 2.0 34.0 2.0 36.0 80-81 20.49382657570532 26.5 2.0 33.5 2.0 35.0 82-83 19.73942860064413 26.0 2.0 33.0 2.0 35.0 84-85 19.033928176025015 25.5 2.0 32.0 2.0 35.0 86-87 18.263779256707913 24.5 2.0 32.0 2.0 34.0 88-89 17.465126346271905 20.5 2.0 31.5 2.0 34.0 90-91 16.64891255026727 18.5 2.0 31.0 2.0 34.0 92-93 15.807303561499847 9.5 2.0 31.0 2.0 34.0 94-95 14.75013279920422 2.0 2.0 30.5 2.0 34.0 96-97 13.651358386424821 2.0 2.0 29.5 2.0 34.0 98-99 12.624285769601933 2.0 2.0 29.0 2.0 34.0 100-101 11.287821269752119 2.0 2.0 27.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 3.725102641609027 1101 2 3.5764125272667684 1101 3 3.3503034784889394 1101 4 2.996704338551549 1101 5 2.4027980470190506 1101 6 2.5212164434102426 1101 7 2.281124166213921 1101 8 2.2318145576249044 1101 9 2.5205281401013977 1101 10-11 3.230595122715428 1101 12-13 3.3324384924101658 1101 14-15 2.711913441775039 1101 16-17 2.7422807188422276 1101 18-19 2.8301696577370166 1101 20-21 2.6989642632010344 1101 22-23 2.874913358030497 1101 24-25 2.9156515034968997 1101 26-27 2.685688342115135 1101 28-29 2.7383541377067324 1101 30-31 2.81181611263678 1101 32-33 2.5960638886828953 1101 34-35 2.91840087534138 1101 36-37 2.8828781925015043 1101 38-39 2.844808977936271 1101 40-41 2.5579302113751474 1101 42-43 2.56824059235678 1101 44-45 2.686864967930049 1101 46-47 2.502342731977656 1101 48-49 2.542279018008717 1101 50-51 2.627510226183123 1101 52-53 2.46849047518176 1101 54-55 2.3883153379605986 1101 56-57 2.51830483634048 1101 58-59 2.4680149058515433 1101 60-61 2.4252153403990917 1101 62-63 2.4433764206565094 1101 64-65 2.4124001908189605 1101 66-67 1.8977938563630552 1101 68-69 2.0897503415342626 1101 70-71 1.8429703500086028 1101 72-73 1.9606474063450783 1101 74-75 1.8144891372904155 1101 76-77 1.858309941150118 1101 78-79 1.4055622263183807 1101 80-81 1.4577681712327006 1101 82-83 1.3214483271456068 1101 84-85 1.250658341562481 1101 86-87 1.4205352281975046 1101 88-89 1.4351921629827835 1101 90-91 1.2273916789216806 1101 92-93 0.9648100769392514 1101 94-95 1.0445950653823726 1101 96-97 0.8241504745814314 1101 98-99 0.5181530076687402 1101 100-101 0.3450074851666809 1102 1 3.49793156728623 1102 2 2.3239975351623734 1102 3 2.790349485568953 1102 4 2.9656239639007786 1102 5 1.8950767314898762 1102 6 2.356662393225438 1102 7 2.3668371349612087 1102 8 2.48651135773596 1102 9 2.4994900416406054 1102 10-11 1.901746883174937 1102 12-13 3.0100493020133037 1102 14-15 3.054378453835362 1102 16-17 2.815245594247859 1102 18-19 2.77321409654175 1102 20-21 2.794052286176381 1102 22-23 2.824062519881714 1102 24-25 2.7940019295124863 1102 26-27 2.5961025685194272 1102 28-29 3.0219901178028685 1102 30-31 3.069663562616828 1102 32-33 2.6204968259345343 1102 34-35 2.958372318848763 1102 36-37 3.0287901565114232 1102 38-39 3.3002555893337053 1102 40-41 2.938289244467267 1102 42-43 2.722062748203861 1102 44-45 2.539969957778556 1102 46-47 2.6135213027864808 1102 48-49 2.597798132312775 1102 50-51 2.5968082006568913 1102 52-53 2.707465586674637 1102 54-55 2.646120652743601 1102 56-57 2.5911812115638995 1102 58-59 2.5805447312805967 1102 60-61 2.662487365665026 1102 62-63 2.6525378705844957 1102 64-65 2.773367706046198 1102 66-67 2.6959241815578423 1102 68-69 2.621257436732691 1102 70-71 2.584061928175089 1102 72-73 2.293029688088012 1102 74-75 2.435244111087421 1102 76-77 2.3191676987984415 1102 78-79 2.24741858271382 1102 80-81 2.326750809579309 1102 82-83 2.2413549030562017 1102 84-85 2.0887485596893995 1102 86-87 1.9045814435132407 1102 88-89 1.8015827004044773 1102 90-91 1.7792135245843035 1102 92-93 1.7365915877761857 1102 94-95 1.5572686493794574 1102 96-97 1.5423810782914966 1102 98-99 1.3139243041096442 1102 100-101 0.8319751283721235 1103 1 3.7284584474195874 1103 2 3.7156727031632073 1103 3 3.3613097601005464 1103 4 3.0604724809786923 1103 5 2.4602965434331274 1103 6 2.6878982846283073 1103 7 2.466440900437256 1103 8 2.4936066797592638 1103 9 2.7231445155852825 1103 10-11 3.452959137251227 1103 12-13 2.598245452753904 1103 14-15 3.052574832484062 1103 16-17 2.5064261473916822 1103 18-19 2.533817195657896 1103 20-21 2.864344138787118 1103 22-23 2.978022803095527 1103 24-25 2.8551872253155004 1103 26-27 2.756442053924623 1103 28-29 2.0845149163755075 1103 30-31 2.7459155378204265 1103 32-33 2.6104366907524614 1103 34-35 2.5082096901349367 1103 36-37 2.9057539647039583 1103 38-39 3.181985987065911 1103 40-41 3.0096631179500513 1103 42-43 2.9670133355556203 1103 44-45 2.775007573469054 1103 46-47 2.669309517549717 1103 48-49 2.6362811759495806 1103 50-51 2.726259734369929 1103 52-53 2.634019594384444 1103 54-55 2.588180714126853 1103 56-57 2.7664507400328766 1103 58-59 2.7501857434530628 1103 60-61 2.8036093681307754 1103 62-63 2.92602233593378 1103 64-65 2.9291470215404765 1103 66-67 2.459294899055976 1103 68-69 2.403481635364372 1103 70-71 2.432929496200231 1103 72-73 2.31925681045513 1103 74-75 2.3909283020787555 1103 76-77 2.3513101218306183 1103 78-79 2.151639115280801 1103 80-81 2.1983575834423768 1103 82-83 2.3600243755249046 1103 84-85 2.0925969508752154 1103 86-87 2.050595981879944 1103 88-89 1.8118639607146072 1103 90-91 1.7041755398997296 1103 92-93 1.5510648921398662 1103 94-95 1.5960606170295275 1103 96-97 1.5083498396869022 1103 98-99 1.4805987124167874 1103 100-101 1.4630372127111446 1104 1 3.7114184093914915 1104 2 3.567747535162372 1104 3 2.8015336960952695 1104 4 2.589966069163939 1104 5 1.7615240999109254 1104 6 2.2849518669096476 1104 7 2.387560819171739 1104 8 2.3453929366833286 1104 9 2.450147936377448 1104 10-11 3.296648198964405 1104 12-13 3.3792927230659338 1104 14-15 2.999773190677466 1104 16-17 2.875936383721541 1104 18-19 2.979464096541747 1104 20-21 2.543065444071118 1104 22-23 2.3870559409343457 1104 24-25 2.4662058768809025 1104 26-27 2.700543357993112 1104 28-29 2.4828453809607574 1104 30-31 1.743512246827354 1104 32-33 2.1757599838292627 1104 34-35 2.7695565293750697 1104 36-37 2.9970467354587917 1104 38-39 2.9861108524915956 1104 40-41 3.041578718151481 1104 42-43 2.8924574850459663 1104 44-45 2.8185883788311905 1104 46-47 2.7388502501549006 1104 48-49 2.3037191849443523 1104 50-51 2.218683200656894 1104 52-53 2.62999848141148 1104 54-55 2.4193114422172854 1104 56-57 2.4551614747217947 1104 58-59 2.6048868365437556 1104 60-61 2.7554149972439745 1104 62-63 2.889215502163438 1104 64-65 2.8694203376251473 1104 66-67 2.704312339452578 1104 68-69 2.6549745419958484 1104 70-71 2.426989559754034 1104 72-73 2.39878626703538 1104 74-75 2.610902005824265 1104 76-77 2.584134804061602 1104 78-79 2.2609054248190787 1104 80-81 2.6022442306319427 1104 82-83 2.503361482003573 1104 84-85 2.3538801386367645 1104 86-87 2.3794169698290304 1104 88-89 2.390234016193954 1104 90-91 2.3708253666895693 1104 92-93 2.332973166723555 1104 94-95 2.3599002283268273 1104 96-97 2.405374499344129 1104 98-99 2.227246672530697 1104 100-101 2.0877317073194916 1105 1 3.5649741738025185 1105 2 3.3397852683843183 1105 3 3.0036170294286 1105 4 2.766798775930855 1105 5 0.011842604505748255 1105 6 1.8384971885470769 1105 7 2.293401810539045 1105 8 2.2292397063853606 1105 9 2.5225407239018125 1105 10-11 2.7256956439657998 1105 12-13 3.346236123233023 1105 14-15 3.1353430180236117 1105 16-17 2.9708890431478814 1105 18-19 2.980765092086159 1105 20-21 2.989539267518623 1105 22-23 2.723164441352054 1105 24-25 2.1954291085712363 1105 26-27 2.6090664017135197 1105 28-29 2.5278580863353106 1105 30-31 2.5978277230178257 1105 32-33 2.678334997752966 1105 34-35 3.050575918124725 1105 36-37 2.8809813985052948 1105 38-39 3.116940530854169 1105 40-41 3.1359431013316517 1105 42-43 2.965912824784205 1105 44-45 2.698512146583905 1105 46-47 2.949794276888401 1105 48-49 2.95270971682962 1105 50-51 3.009336918284294 1105 52-53 2.781894715051134 1105 54-55 2.5735216204406193 1105 56-57 3.0452843513578323 1105 58-59 3.0305708382145973 1105 60-61 3.146645678112815 1105 62-63 2.9858859978470917 1105 64-65 2.9881007191344757 1105 66-67 2.8487176234960216 1105 68-69 3.1150935896148972 1105 70-71 3.0112070479188944 1105 72-73 2.9490743135405317 1105 74-75 2.989288075164282 1105 76-77 3.0257292415441945 1105 78-79 2.7629086968881467 1105 80-81 3.077318200278281 1105 82-83 3.0181771670495188 1105 84-85 2.7974909016554257 1105 86-87 2.8576098478574323 1105 88-89 3.013851218922998 1105 90-91 3.039261213250416 1105 92-93 2.8674961060162296 1105 94-95 3.008372972687729 1105 96-97 2.8761965196727264 1105 98-99 2.646634551951472 1105 100-101 2.3643085661331913 1106 1 3.151606741402425 1106 2 2.6900950181915526 1106 3 2.063081016028267 1106 4 1.4160509893790483 1106 5 1.7842622423776469 1106 6 2.142691772578342 1106 7 2.190899882911488 1106 8 0.6354347025301088 1106 9 1.7735015465334953 1106 10-11 0.8525877101166586 1106 12-13 1.421504329342941 1106 14-15 1.8440984353217402 1106 16-17 1.7905202680554915 1106 18-19 2.1045872451796725 1106 20-21 2.1349420419729057 1106 22-23 2.43926627990993 1106 24-25 2.678327063956626 1106 26-27 2.4268635554717335 1106 28-29 2.121476309727395 1106 30-31 2.168308101098706 1106 32-33 2.278822720759532 1106 34-35 2.5643145850041016 1106 36-37 1.788431014309186 1106 38-39 2.621225846320389 1106 40-41 1.9205293925762348 1106 42-43 2.178399530801059 1106 44-45 2.3717378983586492 1106 46-47 2.7900585945082454 1106 48-49 2.527067285094226 1106 50-51 2.455461389848466 1106 52-53 2.43021585115493 1106 54-55 2.3638620461139617 1106 56-57 2.654695328199711 1106 58-59 2.722867639682253 1106 60-61 2.8690663130334464 1106 62-63 2.9070596571488956 1106 64-65 2.938775887303361 1106 66-67 2.460886603315757 1106 68-69 2.7460549440058983 1106 70-71 2.6411238275844156 1106 72-73 2.9483132330055817 1106 74-75 2.8674173126214058 1106 76-77 2.998110659214557 1106 78-79 2.6382648094615924 1106 80-81 2.6311136324656736 1106 82-83 2.7891790016191926 1106 84-85 2.7084413217452905 1106 86-87 2.911369220115553 1106 88-89 2.8374076585284307 1106 90-91 2.724431941228932 1106 92-93 2.496977673908603 1106 94-95 2.7163907400962 1106 96-97 2.384250513185263 1106 98-99 2.496762838888804 1106 100-101 2.505628758364189 1107 1 3.0334140234265803 1107 2 3.4118264825307953 1107 3 -0.23778647934332753 1107 4 1.0802950165323608 1107 5 1.5933004157004014 1107 6 2.1366404634008767 1107 7 2.1202801174173516 1107 8 2.1455025858061383 1107 9 2.1803233749739377 1107 10-11 1.8151240761573888 1107 12-13 2.283042723065936 1107 14-15 2.686417927519571 1107 16-17 1.2944451556513634 1107 18-19 0.419694359699637 1107 20-21 -1.3105529769815192 1107 22-23 0.6169353268992595 1107 24-25 1.4068089470563443 1107 26-27 1.2828240597474974 1107 28-29 2.3394133634168988 1107 30-31 3.0381723345466476 1107 32-33 1.5984683171625953 1107 34-35 1.0152473188487612 1107 36-37 1.118154191599146 1107 38-39 2.136384975298615 1107 40-41 2.5749559111339373 1107 42-43 1.7107798534670238 1107 44-45 1.5665708349715395 1107 46-47 2.433302004540863 1107 48-49 2.1039384831899675 1107 50-51 2.386490218200752 1107 52-53 1.88068927088516 1107 54-55 2.7342675825681617 1107 56-57 3.1031877905112673 1107 58-59 3.028636836543754 1107 60-61 2.7541540323316944 1107 62-63 2.6671760284792327 1107 64-65 1.9199466534146197 1107 66-67 2.4891040061192484 1107 68-69 2.7634833139256756 1107 70-71 1.311287805368071 1107 72-73 2.2645867056318743 1107 74-75 2.7998822689821594 1107 76-77 2.7875887514300217 1107 78-79 2.826212442362941 1107 80-81 2.7231104587021164 1107 82-83 2.749973324108833 1107 84-85 2.8676082088122037 1107 86-87 2.8508314435132434 1107 88-89 2.8157493670711453 1107 90-91 2.8523275596720232 1107 92-93 2.883356938653378 1107 94-95 2.896270842361913 1107 96-97 2.5921179203967615 1107 98-99 2.6941436023552594 1107 100-101 2.0341132862668605 1108 1 2.4295959525863715 1108 2 2.0525901208722566 1108 3 1.5295671959230326 1108 4 1.6299391233547063 1108 5 1.7875952468188423 1108 6 -0.24219280647723096 1108 7 0.541949654677655 1108 8 -0.7902402331772471 1108 9 1.283003240327993 1108 10-11 2.7192435291128945 1108 12-13 1.7893652206637007 1108 14-15 1.85345801309348 1108 16-17 0.10625302530828051 1108 18-19 0.24581543227429137 1108 20-21 1.2485048035359156 1108 22-23 -0.6985942825187053 1108 24-25 0.9012285734561338 1108 26-27 1.0942458798851646 1108 28-29 1.7152283400593298 1108 30-31 0.5410955658077157 1108 32-33 0.723744770438401 1108 34-35 1.0683091242406384 1108 36-37 -0.1299119347915827 1108 38-39 1.2669338328023478 1108 40-41 -0.02417154926697762 1108 42-43 1.2129915401069766 1108 44-45 1.8899016300957605 1108 46-47 1.6396011747882184 1108 48-49 1.43819191732646 1108 50-51 1.7861855122401913 1108 52-53 1.1142773920665192 1108 54-55 1.573116263001591 1108 56-57 2.2556335362914943 1108 58-59 2.1717309134514906 1108 60-61 1.396555117718254 1108 62-63 1.411163192393147 1108 64-65 1.087349158989614 1108 66-67 1.5905695801473243 1108 68-69 2.2867089546723918 1108 70-71 2.422302484677438 1108 72-73 2.068443393510716 1108 74-75 2.1829569467202496 1108 76-77 2.2812967123416037 1108 78-79 2.1163229148928693 1108 80-81 2.0699703918626113 1108 82-83 2.261014352446125 1108 84-85 2.227418628764948 1108 86-87 1.9024227193259193 1108 88-89 2.120171229512298 1108 90-91 2.068787604030252 1108 92-93 2.1137912745793095 1108 94-95 2.3580589502478855 1108 96-97 2.2988448997466175 1108 98-99 2.0178913889764782 1108 100-101 1.7064014817814819 1109 1 1.427547550655916 1109 2 1.5956287086060783 1109 3 0.8805210322624646 1109 4 2.2377706397029336 1109 5 2.0851161399206255 1109 6 0.7342272038971736 1109 7 0.689546854930633 1109 8 -1.8444688891085654 1109 9 -0.156526560704787 1109 10-11 -0.4181971386756551 1109 12-13 0.9723230705872403 1109 14-15 0.9213328183598506 1109 16-17 0.01826490694244498 1109 18-19 1.4358541001772949 1109 20-21 -0.3261960964699284 1109 22-23 -0.8824028165464952 1109 24-25 0.09628952949010738 1109 26-27 -2.718589889578528 1109 28-29 -1.8700771002555676 1109 30-31 -0.47783583145816166 1109 32-33 -2.1629810554719597 1109 34-35 0.8077400011389706 1109 36-37 2.010872837310128 1109 38-39 2.2561688931165413 1109 40-41 -0.5128256575593149 1109 42-43 -0.012375990446692242 1109 44-45 -1.0124531596590316 1109 46-47 -1.8614495662032127 1109 48-49 0.6395715350012168 1109 50-51 0.5288650713804621 1109 52-53 0.07846916163222062 1109 54-55 0.5856781760017995 1109 56-57 -0.16760569159996308 1109 58-59 -0.4755628763410087 1109 60-61 0.7563493452686565 1109 62-63 -0.9232634069850931 1109 64-65 1.0100844314409834 1109 66-67 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54-55 -1.383303865884983 2312 56-57 -0.64410572325448 2312 58-59 -0.9822347895583654 2312 60-61 -0.494536113471451 2312 62-63 -1.0986687046789072 2312 64-65 -2.3533293737975995 2312 66-67 0.6209851204777053 2312 68-69 -0.3350277911819397 2312 70-71 -3.093125502750567 2312 72-73 -3.3249003227182854 2312 74-75 -1.0834281419027292 2312 76-77 -0.5984428815488307 2312 78-79 -0.5553442374841353 2312 80-81 -1.961832276109714 2312 82-83 -0.19036633745758635 2312 84-85 1.154805120830936 2312 86-87 1.4563701619846405 2312 88-89 1.636796186172095 2312 90-91 2.2410117292653986 2312 92-93 2.7065569124562963 2312 94-95 2.1500447779007157 2312 96-97 1.505290305395656 2312 98-99 0.7806142002218337 2312 100-101 1.4847211404800866 2313 1 -7.926744252698516 2313 2 -4.627728246683546 2313 3 -1.9895894923105288 2313 4 0.20119318586874613 2313 5 0.498428180765238 2313 6 -2.694262473288674 2313 7 -1.258415534756562 2313 8 -2.292679145696532 2313 9 0.38340022470696766 2313 10-11 -1.8510634715161416 2313 12-13 -2.6556186041651806 2313 14-15 -3.8029513516566276 2313 16-17 -3.876049218871902 2313 18-19 -1.1509349309182113 2313 20-21 -3.1672534918556607 2313 22-23 -1.761997739462295 2313 24-25 -2.6502367241869855 2313 26-27 -2.4743064703821744 2313 28-29 -3.1380070217852385 2313 30-31 -4.635835579259606 2313 32-33 -6.066125981083012 2313 34-35 -5.060046350106237 2313 36-37 -2.0548292180118395 2313 38-39 -1.8564139377448647 2313 40-41 -3.6359074565243397 2313 42-43 -2.9523317979441117 2313 44-45 -3.121514595997187 2313 46-47 -1.6451958024766782 2313 48-49 -2.923652573255495 2313 50-51 -3.403728126574226 2313 52-53 -0.6934259083673169 2313 54-55 -2.040950285471503 2313 56-57 -2.4229444558655437 2313 58-59 -1.600068827071805 2313 60-61 -1.9208793391404662 2313 62-63 -3.7968161530615774 2313 64-65 -5.237122854594535 2313 66-67 -4.337606996931861 2313 68-69 -3.1963388638012518 2313 70-71 -2.1964109932589295 2313 72-73 -2.215935319539753 2313 74-75 -3.300577780598317 2313 76-77 -3.1777120685547224 2313 78-79 -3.3776767643494914 2313 80-81 -4.043393641221606 2313 82-83 -2.0834877742893347 2313 84-85 -1.7910903037736077 2313 86-87 -2.9376692239162026 2313 88-89 -3.862230705392623 2313 90-91 -3.822245285484346 2313 92-93 -2.0922246784328156 2313 94-95 -1.0259502674779046 2313 96-97 -0.9844257485582357 2313 98-99 -0.6317183350970677 2313 100-101 -0.3646424345569397 2314 1 -7.748115142928437 2314 2 -4.003411992624407 2314 3 -24.829716303904732 2314 4 -13.356847769376039 2314 5 -7.020161899490702 2314 6 -5.37976100925281 2314 7 -4.664681196336005 2314 8 -3.403535452206942 2314 9 -3.464295206059411 2314 10-11 -14.391509929510498 2314 12-13 -13.062552779190412 2314 14-15 -5.164877043683898 2314 16-17 -3.391054191959732 2314 18-19 -3.21702507982765 2314 20-21 -4.542479726588837 2314 22-23 -4.272457743650158 2314 24-25 -4.992776598997327 2314 26-27 -4.088814392137781 2314 28-29 -3.3462784596984463 2314 30-31 -3.8605098398614217 2314 32-33 -4.1659034364754 2314 34-35 -3.1822392801689183 2314 36-37 -4.043170781183353 2314 38-39 -1.3037460842308306 2314 40-41 -2.2010348473824415 2314 42-43 -4.147029366408816 2314 44-45 -5.113291623163374 2314 46-47 -4.326086079596525 2314 48-49 -2.6567407492350092 2314 50-51 -2.5689374081840164 2314 52-53 -1.0784784969474934 2314 54-55 -3.042006154830762 2314 56-57 -2.9748084198155915 2314 58-59 -1.1322826578340646 2314 60-61 -3.8663197599177614 2314 62-63 -4.935214956087826 2314 64-65 -4.251410398618589 2314 66-67 -5.246993710935115 2314 68-69 -4.356524747440435 2314 70-71 -4.8834127190362615 2314 72-73 -4.147503764503675 2314 74-75 -3.5849627782640994 2314 76-77 -3.3358087404047296 2314 78-79 -4.34667734962057 2314 80-81 -6.581824260853761 2314 82-83 -5.982926274484997 2314 84-85 -4.476370864878653 2314 86-87 -4.047148062333328 2314 88-89 -3.272475802674631 2314 90-91 -5.244518368507022 2314 92-93 -5.2774556802933255 2314 94-95 -5.669591516044763 2314 96-97 -5.081763752045834 2314 98-99 -5.861893779113572 2314 100-101 -4.998727223344449 2315 1 -24.36062106429272 2315 2 -24.472384043784995 2315 3 -13.300333346020304 2315 4 -28.64391550978343 2315 5 -15.66467473765802 2315 6 -12.288835763896063 2315 7 -9.704486451306124 2315 8 -8.962726360703133 2315 9 -6.047037485551517 2315 10-11 -29.01206890629875 2315 12-13 -28.74800990851301 2315 14-15 -23.55503730213144 2315 16-17 -14.380841027485225 2315 18-19 -9.454872886034678 2315 20-21 -7.110045218849656 2315 22-23 -4.060810089531142 2315 24-25 -5.77198858546252 2315 26-27 -5.874568761188289 2315 28-29 -17.458168814358586 2315 30-31 -16.40572563270252 2315 32-33 -9.815937236388269 2315 34-35 -7.631764898275836 2315 36-37 -5.336040182681316 2315 38-39 -5.449592824244316 2315 40-41 -8.691183924252766 2315 42-43 -8.068374742130686 2315 44-45 -5.367788496265465 2315 46-47 -4.268925601693134 2315 48-49 -4.938069695925794 2315 50-51 -3.307731820735963 2315 52-53 -7.039273613799612 2315 54-55 -6.863196032394807 2315 56-57 -7.615372444552396 2315 58-59 -7.5468062275317145 2315 60-61 -5.137446909297598 2315 62-63 -3.579720718775782 2315 64-65 -4.60699081758904 2315 66-67 -6.795038303621805 2315 68-69 -6.698135154380253 2315 70-71 -7.53516957215998 2315 72-73 -6.766556824103338 2315 74-75 -7.37015226888062 2315 76-77 -7.094678375741481 2315 78-79 -7.056529107888789 2315 80-81 -7.963027658382956 2315 82-83 -7.065626449075349 2315 84-85 -6.291780075807276 2315 86-87 -6.673069788509029 2315 88-89 -6.090398200672897 2315 90-91 -5.487913002556269 2315 92-93 -5.538397619141698 2315 94-95 -5.78641417576617 2315 96-97 -5.733513297707265 2315 98-99 -5.3966670923540905 2315 100-101 -5.000151366550297 2316 1 -1.7513020678769493 2316 2 1.1563921978944727 2316 3 1.6249687959416583 2316 4 2.0626897129508244 2316 5 -1.390295132309408 2316 6 -2.6350371176485226 2316 7 -3.0904520894438896 2316 8 -4.1001680423772235 2316 9 -3.6016805595936603 2316 10-11 -3.3633233865854884 2316 12-13 -4.146115387263649 2316 14-15 -2.517669441440461 2316 16-17 -3.0844531654201326 2316 18-19 -4.417105704978184 2316 20-21 -2.1114005259614927 2316 22-23 -2.5314598546028826 2316 24-25 -2.566582326827291 2316 26-27 -2.6702101386517256 2316 28-29 -1.8516648698005547 2316 30-31 0.8022888085272974 2316 32-33 -1.6013042324911027 2316 34-35 -6.984220975543135 2316 36-37 -3.0233218102333943 2316 38-39 -1.1377317681729657 2316 40-41 -0.9449794110424712 2316 42-43 -1.2385695954240212 2316 44-45 -0.6293008334468162 2316 46-47 0.500405516007703 2316 48-49 0.9616809576557017 2316 50-51 1.1302086540753855 2316 52-53 0.8661507741055807 2316 54-55 -0.8863104754801832 2316 56-57 0.468548773614188 2316 58-59 -2.996502894504296 2316 60-61 -2.76160444989538 2316 62-63 -4.48858703509368 2316 64-65 -5.0060802417803565 2316 66-67 -2.6210404410201065 2316 68-69 -2.302437132351308 2316 70-71 -2.977698908729 2316 72-73 -2.8573986340614503 2316 74-75 -2.5275290011286025 2316 76-77 -2.117565262637406 2316 78-79 -2.464951219479836 2316 80-81 -2.8157522895664044 2316 82-83 -3.841653862136349 2316 84-85 -3.223177087762565 2316 86-87 -2.53843149878875 2316 88-89 -3.7136566749155406 2316 90-91 -4.1037826082208255 2316 92-93 -4.502570244896894 2316 94-95 -5.6946640216799125 2316 96-97 -5.565266400620837 2316 98-99 -5.8574830449080615 2316 100-101 -5.680350292033731 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 39121.0 3 14022.0 4 12687.0 5 13197.0 6 15798.0 7 19132.0 8 24054.0 9 28615.0 10 33340.0 11 38488.0 12 42874.0 13 47647.0 14 51244.0 15 55658.0 16 58965.0 17 59289.0 18 56270.0 19 57545.0 20 61303.0 21 65990.0 22 71571.0 23 77937.0 24 84332.0 25 91505.0 26 98832.0 27 106453.0 28 113315.0 29 121765.0 30 129657.0 31 138573.0 32 146178.0 33 151697.0 34 155395.0 35 151221.0 36 130892.0 37 80043.0 38 15294.0 39 132.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.93779875402948 19.718609301708913 14.991303624705706 21.3522883195559 2 15.488516829180057 21.108067904642542 40.35736122278596 23.046054043391443 3 19.922547397501482 26.074318067973103 30.048611848995886 23.95452268552953 4 12.510494586044988 17.41414916684173 38.32918606000438 31.746170187108895 5 13.068969815232728 38.22095931971965 33.9904827928488 14.719588072198828 6 30.100526243501918 36.91102402056056 18.00362368603752 14.984826049900002 7 27.604008308623207 33.91999187199904 21.116094947092733 17.35990487228502 8 26.223858989165954 33.71285804120092 19.996304479294736 20.066978490338396 9 26.69926258416159 17.61395023985959 21.34155795089012 34.345229225088694 10-11 24.85176271020518 27.13122891064999 27.122930834579957 20.894077544564873 12-13 26.786344124111082 24.94621751435025 27.41651716506881 20.85092119646986 14-15 23.322230846226365 25.919676373802915 27.239205675698702 23.518887104272018 16-17 21.443013811874568 29.446819494774335 27.46806381461199 21.642102878739106 18-19 21.09756625304571 27.82603460116466 30.58223656359069 20.494162582198932 20-21 22.694727800577173 26.183562077984845 30.33329857905175 20.788411542386235 22-23 22.013718433297694 26.515371120514402 30.412720044573614 21.058190401614286 24-25 21.99962725482592 26.608470728208527 30.729902822697547 20.661999194268006 26-27 21.652002621945503 26.637351127221958 30.992758776872208 20.71788747396032 28-29 20.892533146320414 27.391855753623705 30.858870442717564 20.85674065733832 30-31 21.30287704132995 26.687137243883967 30.81086760924724 21.199118105538844 32-33 21.250073664100363 26.94172449494669 30.634174004677977 21.17402783627497 34-35 22.153410410761275 26.728717146119692 30.328238552744473 20.78963389037456 36-37 21.840711955628695 26.409957229733532 30.646063857440225 21.103266957197548 38-39 21.317191899407806 27.090555173410998 30.722793089987366 20.869459837193833 40-41 21.574692274858716 26.988328614542407 30.551304336043156 20.885674774555717 42-43 21.322126871437167 27.11139818604526 30.01086129443819 21.55561364807938 44-45 21.66444858386188 26.946459414731045 29.794319637683653 21.594772363723422 46-47 22.044074370087827 27.311717162666778 29.35978583607244 21.28442263117296 48-49 21.92317264692604 27.4003841292349 29.21366463883681 21.462778585002244 50-51 21.363691908177156 27.704995600684086 29.37630259008901 21.555009901049754 52-53 21.75958132378669 28.154694759202798 28.59570589464922 21.490018022361287 54-55 21.3517142985738 27.62920538118551 28.852282551974707 22.16679776826598 56-57 21.784720127807315 27.837362705219714 28.606921339988773 21.770995826984198 58-59 22.295335493351757 27.43298066919662 28.581606513861647 21.690077323589975 60-61 21.827428075899036 27.69916270778931 29.25402104258425 21.219388173727406 62-63 21.55037560477299 27.627709010374208 29.201737289698592 21.620178095154213 64-65 21.34300048383427 27.9869238660146 28.842092941721774 21.82798270842935 66-67 21.519650507105307 27.877910833395276 28.245393993396668 22.357044666102745 68-69 21.36615525196299 27.714150712438514 28.752253626266018 22.16744040933248 70-71 21.698696281262375 27.748326123909738 28.595464763158013 21.95751283166987 72-73 21.70174126595326 27.301812341874427 28.96377669135187 22.032669700820442 74-75 21.2218331129887 28.324761447049614 28.386902210467994 22.06650322949369 76-77 21.555412497549888 27.728721099714797 28.300574451744005 22.415291950991314 78-79 21.52866457913992 27.867069861850418 28.315844177363203 22.288421381646458 80-81 21.51262080130386 27.954992982481247 28.256461973031787 22.275924243183105 82-83 21.55448530879213 27.75406959449028 28.43557369703314 22.255871399684448 84-85 21.319024266090217 27.89535816875666 28.24486456580052 22.540752999352605 86-87 21.33301707779886 27.815521369838258 28.411437155507365 22.440024396855517 88-89 21.414160238237763 27.738115406769897 27.94591460636932 22.901809748623016 90-91 21.34994059556546 27.576270832262622 28.294527017016346 22.77926155515557 92-93 21.65060719050903 27.69854918815376 28.2486649940817 22.402178627255505 94-95 21.29307743796762 27.372105366050636 28.28694473333602 23.04787246264572 96-97 21.115213167563542 27.387837572125 28.334512344967504 23.16243691534395 98-99 21.15518796448601 27.588142613546886 27.664641759453986 23.59202766251312 100-101 21.010932956691743 27.296893159101426 28.159410437333342 23.53276344687349 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2860.0 1 1800.5 2 731.0 3 721.5 4 717.5 5 757.5 6 1065.5 7 1904.5 8 2954.5 9 3575.0 10 3541.0 11 3250.0 12 3270.5 13 3495.0 14 3728.0 15 4015.5 16 4365.5 17 4718.5 18 5115.5 19 5639.0 20 6352.0 21 7349.0 22 8518.0 23 9983.0 24 11699.0 25 13930.0 26 17392.5 27 21501.5 28 25025.0 29 28915.5 30 33853.0 31 39673.0 32 46363.5 33 53635.5 34 61567.0 35 68371.0 36 75080.5 37 81550.0 38 84678.5 39 85672.5 40 86337.0 41 87916.5 42 89729.5 43 91415.0 44 93836.0 45 95832.0 46 97385.5 47 102775.5 48 112562.5 49 112664.5 50 101543.5 51 91081.0 52 83253.5 53 77354.0 54 72232.0 55 66128.5 56 59701.0 57 53612.5 58 47677.0 59 41788.0 60 35474.5 61 28983.5 62 23182.0 63 18236.5 64 13785.0 65 10293.5 66 7647.5 67 5647.0 68 4003.5 69 2735.5 70 1923.0 71 1411.0 72 1080.5 73 805.5 74 583.0 75 446.0 76 336.5 77 237.5 78 159.0 79 105.5 80 71.5 81 42.5 82 30.0 83 23.0 84 15.0 85 9.0 86 10.0 87 6.5 88 1.5 89 2.5 90 4.0 91 1.5 92 1.0 93 1.5 94 1.0 95 0.5 96 0.5 97 0.5 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.161342480595151 2 2.4229040939748447 3 0.9349891035104477 4 0.8630350548546238 5 0.16616347704218484 6 0.09458536385478215 7 0.09590113799425645 8 0.10375818928426023 9 0.09935974430373179 10-11 1.690469020849757 12-13 1.302278056157992 14-15 0.4897123379389187 16-17 0.09122074141241211 18-19 0.09796878307057325 20-21 0.1234572078295328 22-23 0.14101339420480438 24-25 0.15266739372586258 26-27 0.14992306480638762 28-29 0.5347494070557824 30-31 0.1818775796221924 32-33 0.16524243514455283 34-35 0.14714114234007047 36-37 0.1364457782634864 38-39 0.42243868586493916 40-41 0.12319405300163795 42-43 0.1259195851476919 44-45 0.12997968820664121 46-47 0.14090061356427802 48-49 0.15616359358218004 50-51 0.16934013175034426 52-53 0.18781736002324784 54-55 0.18667075684456308 56-57 0.15631396776954856 58-59 0.14009235230717237 60-61 0.11778058225637221 62-63 0.1221414336900585 64-65 0.11764900484242477 66-67 0.11422799207979155 68-69 0.1099423277397895 70-71 0.12492335615637562 72-73 0.1397164168387511 74-75 0.1490396164555977 76-77 0.17076868653034494 78-79 0.17202807034955606 80-81 0.1704491413821869 82-83 0.16394545777849956 84-85 0.15035539059507202 86-87 0.14853210357322905 88-89 0.14514868435743794 90-91 0.15355084207665248 92-93 0.1452238714511222 94-95 0.19729093382746288 96-97 0.197930024123779 98-99 0.21627567498273517 100-101 0.24071148043011528 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2660031.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.827270564376114 #Duplication Level Percentage of deduplicated Percentage of total 1 81.20244397321046 50.205254740202754 2 10.73953086281527 13.279917607794948 3 3.5794774490400396 6.63927962162644 4 1.5926686668905863 3.93881426348994 5 0.8164018285873721 2.5237948372661427 6 0.4876181460797824 1.8088859429864508 7 0.3161782590906282 1.3683907139958777 8 0.1959139429456331 0.9690259486266722 9 0.147439270423001 0.8204190897831389 >10 0.8256116437001488 9.33273907895926 >50 0.06262805833960292 2.6788741442252655 >100 0.031535963173553117 3.5080017754645985 >500 0.0012432507275218845 0.5329296550914466 >1k 0.0010469479810693908 1.2183792220992844 >5k 1.9630274645051076E-4 0.7670497426949937 >10k+ 6.543424881683692E-5 0.4082436156927839 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10091 0.3793564811838659 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7214 0.2711998469190773 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6586 0.24759109950222385 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5160 0.19398270170535606 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4308 0.16195299979586703 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3024 0.11368288565058075 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2683 0.10086348617741672 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 3.759354684212327E-5 0.0 4 0.0 0.0 0.0 3.759354684212327E-5 0.0 5 0.0 0.0 0.0 7.518709368424654E-5 0.0 6 0.0 0.0 0.0 7.518709368424654E-5 0.0 7 0.0 0.0 0.0 7.518709368424654E-5 0.0 8 0.0 0.0 0.0 7.518709368424654E-5 0.0 9 0.0 0.0 0.0 7.518709368424654E-5 0.0 10-11 0.0 0.0 0.0 1.127806405263698E-4 0.0 12-13 0.0 0.0 0.0 1.127806405263698E-4 0.0 14-15 0.0 0.0 0.0 1.127806405263698E-4 0.0 16-17 0.0 0.0 0.0 1.127806405263698E-4 0.0 18-19 0.0 0.0 0.0 1.691709607895547E-4 0.0 20-21 0.0 0.0 0.0 2.631548278948629E-4 0.0 22-23 1.8796773421061635E-5 0.0 0.0 4.1352901526335595E-4 0.0 24-25 3.759354684212327E-5 0.0 0.0 6.20293522895034E-4 0.0 26-27 3.759354684212327E-5 1.8796773421061635E-5 0.0 9.962289913162665E-4 0.0 28-29 3.759354684212327E-5 3.759354684212327E-5 0.0 0.002105238623158903 0.0 30-31 3.759354684212327E-5 7.518709368424654E-5 0.0 0.005920983627634414 0.0 32-33 3.759354684212327E-5 7.518709368424654E-5 0.0 0.014605092948164889 0.0 34-35 3.759354684212327E-5 7.518709368424654E-5 0.0 0.02868387624054005 0.0 36-37 3.759354684212327E-5 7.518709368424654E-5 0.0 0.0505633205026558 0.0 38-39 3.759354684212327E-5 7.518709368424654E-5 0.0 0.08311933206793454 0.0 40-41 3.759354684212327E-5 7.518709368424654E-5 0.0 0.12044972408216295 0.0 42-43 3.759354684212327E-5 7.518709368424654E-5 0.0 0.1596785902119186 0.0 44-45 3.759354684212327E-5 7.518709368424654E-5 0.0 0.20642616571009886 0.0 46-47 3.759354684212327E-5 7.518709368424654E-5 0.0 0.2674216954614439 0.0 48-49 3.759354684212327E-5 7.518709368424654E-5 0.0 0.33215778312358013 0.0 50-51 3.759354684212327E-5 7.518709368424654E-5 0.0 0.40245771571835065 0.0 52-53 3.759354684212327E-5 7.518709368424654E-5 0.0 0.4692050581365405 0.0 54-55 3.759354684212327E-5 7.518709368424654E-5 0.0 0.5457455195071035 0.0 56-57 3.759354684212327E-5 7.518709368424654E-5 0.0 0.6399549478934644 0.0 58-59 3.759354684212327E-5 7.518709368424654E-5 0.0 0.744746959715883 0.0 60-61 3.759354684212327E-5 7.518709368424654E-5 0.0 0.8588245776083061 0.0 62-63 3.759354684212327E-5 7.518709368424654E-5 0.0 0.9791051307296794 3.759354684212327E-5 64-65 3.759354684212327E-5 9.398386710530817E-5 0.0 1.1050999029710558 3.759354684212327E-5 66-67 3.759354684212327E-5 1.127806405263698E-4 0.0 1.245774955254281 3.759354684212327E-5 68-69 3.759354684212327E-5 1.127806405263698E-4 0.0 1.4117692613356763 3.759354684212327E-5 70-71 7.518709368424654E-5 1.127806405263698E-4 0.0 1.594699460269448 3.759354684212327E-5 72-73 7.518709368424654E-5 1.127806405263698E-4 0.0 1.7782875462729568 3.759354684212327E-5 74-75 7.518709368424654E-5 1.127806405263698E-4 0.0 1.9690559997233115 3.759354684212327E-5 76-77 7.518709368424654E-5 1.127806405263698E-4 0.0 2.1737716590520937 3.759354684212327E-5 78-79 7.518709368424654E-5 1.127806405263698E-4 0.0 2.419445487665369 3.759354684212327E-5 80-81 7.518709368424654E-5 1.127806405263698E-4 0.0 2.680325905976284 3.759354684212327E-5 82-83 7.518709368424654E-5 1.127806405263698E-4 0.0 2.9407364049516715 3.759354684212327E-5 84-85 7.518709368424654E-5 1.127806405263698E-4 0.0 3.208195693959958 3.759354684212327E-5 86-87 7.518709368424654E-5 1.127806405263698E-4 0.0 3.48567366320167 3.759354684212327E-5 88-89 7.518709368424654E-5 1.127806405263698E-4 0.0 3.7873054862894455 3.759354684212327E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 13275 0.0 25.306812 1 TACATGG 13635 0.0 23.97376 2 GAGTACT 10910 0.0 22.54715 12-13 ACATGGG 14175 0.0 22.520077 3 GTATCAA 26010 0.0 22.453562 1 AGTACTT 11430 0.0 21.458332 12-13 GTACTTT 11825 0.0 20.703985 14-15 AGAGTAC 19025 0.0 20.087381 10-11 CATGGGA 9730 0.0 19.693419 4 TATCAAC 29950 0.0 19.21824 2 CATGGGG 4865 0.0 19.007578 4 GTATCGA 345 5.16593E-10 18.922134 1 ATCAACG 30995 0.0 18.830069 3 GGTATCA 20395 0.0 18.688047 1 ACTTTTT 14865 0.0 18.573296 16-17 TCAACGC 31105 0.0 18.557602 4 CAACGCA 31125 0.0 18.456831 5 AACGCAG 31690 0.0 18.036303 6 TACTTTT 13860 0.0 17.852394 14-15 ATGGGAG 4015 0.0 17.266272 5 >>END_MODULE