FastQCFastQC Report
Fri 27 May 2016
SRR939444_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939444_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2660031
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138920.5222495527307764No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119070.44762636224916175No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT103540.3892435840033443No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80690.3033423294690927No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT63860.24007239013379922No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT45130.1696596768985023No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC40210.15116365185217764No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33970.12770527862269274No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT31290.1176302080690037No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT29960.1126302663390013No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC27880.10481080859583967No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC27810.10454765376794481No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCATA4000.024.60216164-65
CGCATAG4100.024.59033266-67
GTACATG151350.024.0498031
TACATGG154250.023.2271462
CGCGGAA3000.022.12403182-83
ACATGGG154850.022.0898043
GAGTACT127550.021.5937812-13
CGATCGT3300.021.54958324-25
TCGCGGA3150.021.07050782-83
ATTCGCG3150.021.0703180-81
GTACTTT133500.020.61360514-15
AGTACTT132700.020.20201512-13
CTCGCGC3300.019.42219260-61
CGCGCTT3000.019.16429762-63
GTATCAA292400.019.1515181
TCGCGCT3350.019.13230960-61
CATGGGA102850.019.0082654
AGAGTAC224250.018.81617710-11
GGTATCA234800.018.7159331
AACGCAT5300.018.56766964-65
Produced by FastQC (version 0.11.5)