##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939444_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2660031 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.879627342688863 31.0 31.0 31.0 28.0 34.0 2 31.116836608295166 31.0 31.0 34.0 28.0 34.0 3 31.12256962418859 31.0 31.0 34.0 28.0 34.0 4 35.03836797390707 35.0 35.0 37.0 32.0 37.0 5 34.61246090741048 35.0 35.0 37.0 30.0 37.0 6 34.67906727402801 35.0 35.0 37.0 31.0 37.0 7 34.51789509220006 35.0 35.0 37.0 30.0 37.0 8 34.42598150171934 35.0 35.0 37.0 30.0 37.0 9 35.90466802830493 37.0 35.0 39.0 30.0 39.0 10-11 35.700805554521736 37.0 35.0 39.0 30.0 39.0 12-13 35.691873327792045 37.0 35.0 39.0 30.0 39.0 14-15 36.486946392730005 38.0 35.0 40.0 30.0 41.0 16-17 36.42003758602813 38.0 35.0 40.0 30.0 41.0 18-19 36.44176496439327 38.0 35.0 40.0 30.0 41.0 20-21 36.33083806166169 38.0 34.5 40.0 29.5 41.0 22-23 36.173057569629826 38.0 34.0 40.0 29.0 41.0 24-25 36.00788411864373 38.0 34.0 40.0 29.0 41.0 26-27 35.546787612625565 38.0 34.0 40.0 27.0 41.0 28-29 35.355530631034 38.0 34.0 40.0 27.0 41.0 30-31 35.05682809711617 37.5 33.0 40.0 27.0 41.0 32-33 34.81461644619931 37.0 33.0 40.0 26.0 41.0 34-35 35.0508871889087 37.5 33.0 40.0 27.0 41.0 36-37 34.886200010451006 38.0 33.0 40.0 25.5 41.0 38-39 34.70237376932825 37.5 33.0 40.0 25.0 41.0 40-41 34.56852946450624 37.0 33.0 40.0 25.0 41.0 42-43 34.389469521219866 37.0 33.0 40.0 24.5 41.0 44-45 33.99278091119991 37.0 33.0 40.0 24.0 41.0 46-47 33.553673058697434 37.0 32.0 40.0 23.0 41.0 48-49 33.14380527896103 37.0 31.0 40.0 20.5 41.0 50-51 32.89473205387456 37.0 31.0 39.0 19.0 40.5 52-53 32.80258406762928 36.5 31.0 39.0 19.0 40.0 54-55 32.47621381104206 36.0 31.0 39.0 18.0 40.0 56-57 31.988964790259963 36.0 30.0 39.0 15.0 40.0 58-59 31.54284950062612 35.0 29.0 39.0 13.0 40.0 60-61 31.157180686991993 35.0 29.0 38.0 10.0 40.0 62-63 31.022679810874386 35.0 29.0 38.0 9.0 40.0 64-65 30.58282403475749 34.0 28.0 38.0 8.0 40.0 66-67 29.69559433705848 34.0 27.0 37.5 6.0 39.5 68-69 26.22414964336882 31.0 18.0 36.0 2.0 39.0 70-71 24.88228257490232 29.5 12.5 35.0 2.0 38.0 72-73 25.91219745183421 30.5 19.5 35.0 2.0 38.0 74-75 26.67431920906185 31.0 22.5 35.0 2.0 37.5 76-77 26.339863520387546 30.5 23.0 34.0 2.0 36.5 78-79 27.46231510083905 32.0 26.0 35.0 2.0 37.0 80-81 27.45482251898568 32.0 26.0 35.0 2.0 36.5 82-83 27.121663055806494 32.0 25.5 35.0 2.0 36.0 84-85 26.745318005692415 32.0 25.0 34.5 2.0 36.0 86-87 26.322619736386528 32.0 24.5 34.0 2.0 35.0 88-89 25.836183676054905 31.5 23.5 34.0 2.0 35.0 90-91 25.23687618678128 31.0 20.5 34.0 2.0 35.0 92-93 24.665620438258053 31.0 19.0 34.0 2.0 35.0 94-95 23.81348206092335 31.0 11.0 34.0 2.0 35.0 96-97 23.139825626092325 30.0 3.5 34.0 2.0 35.0 98-99 22.296499740040623 30.0 2.0 34.0 2.0 35.0 100-101 20.883958119285076 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.26627343916908686 1101 2 0.13423437815072958 1101 3 0.23381426031302865 1101 4 0.25196580377433264 1101 5 0.44785182194318196 1101 6 0.3313844601999776 1101 7 0.4907466645152141 1101 8 0.6250254079352615 1101 9 0.5249156917917617 1101 10-11 0.6117501814648634 1101 12-13 0.6171862001837027 1101 14-15 0.6004974331219373 1101 16-17 0.5732599385866521 1101 18-19 0.5695325068484252 1101 20-21 0.5050530856143638 1101 22-23 0.5086268577929971 1101 24-25 0.5070521533768044 1101 26-27 0.6478336127398521 1101 28-29 0.6521811323057989 1101 30-31 0.3789960564751027 1101 32-33 0.6643499762818053 1101 34-35 0.6614316757904177 1101 36-37 0.7866397623778951 1101 38-39 0.8628563707435433 1101 40-41 0.7998972901808088 1101 42-43 0.4063037989033518 1101 44-45 0.7133666182924259 1101 46-47 0.4792952517674607 1101 48-49 0.6245582577114348 1101 50-51 0.37982659081055914 1101 52-53 0.2048277235598448 1101 54-55 0.22672049067693933 1101 56-57 0.4262158597888721 1101 58-59 0.3240035723017556 1101 60-61 0.3284699856531681 1101 62-63 -0.08226092648535044 1101 64-65 -0.05281026997111482 1101 66-67 -0.2018806132804798 1101 68-69 -7.465827446248213 1101 70-71 -8.115471495463748 1101 72-73 -5.191769360001647 1101 74-75 -3.213931838928403 1101 76-77 -0.4935923982132451 1101 78-79 -0.2038973344713071 1101 80-81 -0.4846041652286033 1101 82-83 -0.701150535075687 1101 84-85 -0.8394966778744397 1101 86-87 -0.9245457536074291 1101 88-89 -0.9822494620389008 1101 90-91 -0.9349650821612272 1101 92-93 -1.0020684453155155 1101 94-95 -0.9907621754195688 1101 96-97 -0.9243127227811136 1101 98-99 -1.0771239729296447 1101 100-101 -1.1877427838407861 1102 1 0.319945979818435 1102 2 0.322843884111478 1102 3 0.40049914760544425 1102 4 0.3178088547747322 1102 5 0.49257044342270007 1102 6 0.34526857702022085 1102 7 0.45035294716050345 1102 8 0.5526752075083792 1102 9 0.6263900440918988 1102 10-11 0.6124635514997436 1102 12-13 0.547355543547063 1102 14-15 0.7134601068686166 1102 16-17 0.6472684328688416 1102 18-19 0.5081237379553727 1102 20-21 0.6757912487149937 1102 22-23 0.5951948944771885 1102 24-25 0.5874303289715783 1102 26-27 0.6511756876352948 1102 28-29 0.4780014942888968 1102 30-31 0.5151648662344144 1102 32-33 0.65181179973262 1102 34-35 0.4403599017088311 1102 36-37 0.6622852388906466 1102 38-39 0.8272411209474413 1102 40-41 0.5078414287790451 1102 42-43 0.3893572612560732 1102 44-45 0.4855019671740166 1102 46-47 0.5126362312562449 1102 48-49 0.5044471335855931 1102 50-51 0.42462753047426105 1102 52-53 0.2192732091829015 1102 54-55 0.029312428917698696 1102 56-57 0.20014176474662193 1102 58-59 0.3275129949682132 1102 60-61 0.4423022604549125 1102 62-63 0.1622584611280935 1102 64-65 0.07834586126945098 1102 66-67 -0.09430001954837763 1102 68-69 -6.927793408384016 1102 70-71 -7.760087029414848 1102 72-73 -1.6312503649993069 1102 74-75 -0.02491464646653796 1102 76-77 0.44918355750195715 1102 78-79 0.1766522017703842 1102 80-81 -0.17475597123967646 1102 82-83 -0.17185193155650325 1102 84-85 -0.3767738548053927 1102 86-87 -0.5583496237550989 1102 88-89 -0.5243241948030573 1102 90-91 -0.699976166375226 1102 92-93 -0.5059403282870392 1102 94-95 -0.5136435938518993 1102 96-97 -0.6655048397575349 1102 98-99 -0.40400763857248734 1102 100-101 -0.36404723965142693 1103 1 0.29406582041299245 1103 2 0.19080477942134522 1103 3 0.3679781800595592 1103 4 0.2857912385633057 1103 5 0.43004114095174373 1103 6 0.4847795947492983 1103 7 0.42243322724871035 1103 8 0.4699558513813358 1103 9 0.4618076151558128 1103 10-11 0.5277921624323838 1103 12-13 0.45932082771590643 1103 14-15 0.5219843041979786 1103 16-17 0.588060728272481 1103 18-19 0.5990308013702617 1103 20-21 0.5560457786599429 1103 22-23 0.5766697638098748 1103 24-25 0.5263197544695046 1103 26-27 0.578298814447713 1103 28-29 0.41920814833741105 1103 30-31 0.4442311809820225 1103 32-33 0.6898357713244181 1103 34-35 0.49327012544947735 1103 36-37 0.5608573945974626 1103 38-39 0.6186908769772401 1103 40-41 0.5420710017009469 1103 42-43 0.4455428500837968 1103 44-45 0.5715660375694114 1103 46-47 0.5826928501417967 1103 48-49 0.5954672285690066 1103 50-51 0.18380973106812348 1103 52-53 0.14936142047325518 1103 54-55 0.05872977641461574 1103 56-57 0.338174689871213 1103 58-59 0.39032598876910996 1103 60-61 0.2688196943398573 1103 62-63 -0.03483093528441472 1103 64-65 -0.07658503916292503 1103 66-67 -0.08195743893402252 1103 68-69 -7.133935423352153 1103 70-71 -7.884795035718135 1103 72-73 -1.1632759579944967 1103 74-75 0.37815353929810414 1103 76-77 0.5490819756026362 1103 78-79 0.24478809794847578 1103 80-81 0.12373457165914203 1103 82-83 -0.06511035547426758 1103 84-85 -0.30970239801677124 1103 86-87 -0.33487579411749735 1103 88-89 -0.2977599312284127 1103 90-91 -0.33430205760445375 1103 92-93 -0.26572301248020125 1103 94-95 -0.1510289886321523 1103 96-97 -0.08762760842863315 1103 98-99 0.10301236524768953 1103 100-101 0.27164973589945873 1104 1 0.23310387455527604 1104 2 0.15902809463779377 1104 3 0.22681493707912637 1104 4 0.20004569687999663 1104 5 0.4317151802648027 1104 6 0.2219133138623235 1104 7 0.29410294716050345 1104 8 0.5082673127715367 1104 9 0.5073110967234769 1104 10-11 0.4348319725523808 1104 12-13 0.32909896459968735 1104 14-15 0.5892824752896715 1104 16-17 0.3980908012898965 1104 18-19 0.4365776853237904 1104 20-21 0.3300675645044677 1104 22-23 0.45276068395087776 1104 24-25 0.39779217107684417 1104 26-27 0.5843993718458265 1104 28-29 0.5470804416573145 1104 30-31 0.4450990767607266 1104 32-33 0.5631604839431432 1104 34-35 0.28246516486672135 1104 36-37 0.6660681336274834 1104 38-39 0.5849713841053372 1104 40-41 0.590736165621145 1104 42-43 0.5849164717823854 1104 44-45 0.6287585461213894 1104 46-47 0.6772743891509805 1104 48-49 0.5784602914803258 1104 50-51 0.24485779363215698 1104 52-53 0.26680610391974824 1104 54-55 0.28145058681243285 1104 56-57 0.3368193963255699 1104 58-59 0.49149325812610556 1104 60-61 0.5977298920338576 1104 62-63 0.2602847769175689 1104 64-65 0.14972744021681805 1104 66-67 0.10553550676740997 1104 68-69 -6.7853591978577015 1104 70-71 -4.231633082046425 1104 72-73 0.06315752973753419 1104 74-75 0.7953156166913544 1104 76-77 0.9911243469756421 1104 78-79 0.4572443070335446 1104 80-81 0.269980870865588 1104 82-83 0.17272043686454808 1104 84-85 0.281614303089345 1104 86-87 0.18770300782384552 1104 88-89 0.1889981736179962 1104 90-91 0.057260675730034905 1104 92-93 0.05606625066032933 1104 94-95 0.33668535351652196 1104 96-97 0.20719252866352278 1104 98-99 0.3997752561643573 1104 100-101 0.6412159182433079 1105 1 0.1502265423358402 1105 2 0.23314045888173496 1105 3 0.20634361989728234 1105 4 0.25406964564079004 1105 5 0.36123516077162776 1105 6 0.23440461155099257 1105 7 0.24066031279681255 1105 8 0.34920820667295516 1105 9 0.3011185319782754 1105 10-11 0.43842167597760806 1105 12-13 0.33983674655457463 1105 14-15 0.3502064867071013 1105 16-17 0.38092375172153226 1105 18-19 0.4420827674736145 1105 20-21 0.4610765104805168 1105 22-23 0.4945961754574171 1105 24-25 0.5257526973926332 1105 26-27 0.6430651334721134 1105 28-29 0.4275994475052727 1105 30-31 0.3489942313137817 1105 32-33 0.4384450495237644 1105 34-35 0.46561836035544246 1105 36-37 0.6982440595534101 1105 38-39 0.5853116444784874 1105 40-41 0.6399694919926304 1105 42-43 0.5268233222418601 1105 44-45 0.634468132587557 1105 46-47 0.6320867673186186 1105 48-49 0.5736200659164226 1105 50-51 0.45445557280230275 1105 52-53 0.33262777615588845 1105 54-55 0.38162811396364305 1105 56-57 0.567721303872208 1105 58-59 0.692011045650478 1105 60-61 0.6595380582828163 1105 62-63 0.3952262973854026 1105 64-65 0.39865270476708403 1105 66-67 0.5543833207468083 1105 68-69 -2.996303224591202 1105 70-71 -3.217054970099891 1105 72-73 0.9293899510686394 1105 74-75 1.301609998479158 1105 76-77 1.2092208033944694 1105 78-79 0.8096922672117657 1105 80-81 0.5563800284818505 1105 82-83 0.5530723583348909 1105 84-85 0.46494415549814505 1105 86-87 0.5387655600377137 1105 88-89 0.5270411978452323 1105 90-91 0.5219606200352445 1105 92-93 0.35958285467035367 1105 94-95 0.39441996783008193 1105 96-97 0.44629462418008714 1105 98-99 0.3839274769942058 1105 100-101 0.5484954016741099 1106 1 0.12815919501723627 1106 2 0.1570676344420754 1106 3 0.17003542548607342 1106 4 0.1744440042061015 1106 5 0.323020941527254 1106 6 0.2605555381416167 1106 7 0.16565698930102712 1106 8 0.08419464681014688 1106 9 0.18564134445335156 1106 10-11 0.21495214578697386 1106 12-13 0.1075518099668713 1106 14-15 0.266708806507161 1106 16-17 0.26912883135248933 1106 18-19 0.3421703415875683 1106 20-21 0.23785458072593002 1106 22-23 0.21690597179360083 1106 24-25 0.35666768901742785 1106 26-27 0.49833968745897295 1106 28-29 0.19995776687991906 1106 30-31 0.46558362670342746 1106 32-33 0.7631569024391354 1106 34-35 0.5496247015854081 1106 36-37 0.6042904954365369 1106 38-39 0.5990122573311183 1106 40-41 0.3892388214441169 1106 42-43 0.3669086696136432 1106 44-45 0.5429674745353879 1106 46-47 0.6883588685655937 1106 48-49 0.9633529565601151 1106 50-51 0.9059403445446108 1106 52-53 0.7214639876365752 1106 54-55 0.6095290272602867 1106 56-57 0.8392922870952084 1106 58-59 1.0203378649320634 1106 60-61 1.1558734056545923 1106 62-63 0.5528302895685435 1106 64-65 0.6870651705350745 1106 66-67 0.6197915457341772 1106 68-69 -2.7501981954773775 1106 70-71 -3.3115088865073297 1106 72-73 0.9096457273043193 1106 74-75 1.3385501556140404 1106 76-77 1.1423555578545965 1106 78-79 0.8660099003510062 1106 80-81 0.7901003828994426 1106 82-83 0.6828048501590942 1106 84-85 0.49244807722316963 1106 86-87 0.5310493998718044 1106 88-89 0.607869944754377 1106 90-91 0.7009247196337682 1106 92-93 0.6392086622798274 1106 94-95 0.9223202164275683 1106 96-97 0.8843378495662826 1106 98-99 1.0908283836438564 1106 100-101 1.036896899906008 1107 1 0.0013056289412425315 1107 2 0.08705441042726747 1107 3 0.1729552879563201 1107 4 -0.022410443470882058 1107 5 0.29362307500164775 1107 6 0.20667208579214957 1107 7 0.13555031558155406 1107 8 0.12206117242065773 1107 9 0.15783741251294714 1107 10-11 0.06486486728921648 1107 12-13 0.24566694705583103 1107 14-15 0.03363554546510983 1107 16-17 0.04652281883375764 1107 18-19 0.1857443519904578 1107 20-21 0.24106537152200502 1107 22-23 0.2771466488631589 1107 24-25 0.2985816447610574 1107 26-27 0.5243116525475813 1107 28-29 0.4281769328853855 1107 30-31 0.35218241009405915 1107 32-33 0.2860661856975284 1107 34-35 0.21429630521760856 1107 36-37 0.3028005897678341 1107 38-39 0.3789955069123536 1107 40-41 0.29335677965623574 1107 42-43 0.2195217349402796 1107 44-45 0.4181116162968266 1107 46-47 0.5942700031860682 1107 48-49 0.5434164318312042 1107 50-51 0.3179389339830294 1107 52-53 0.1671679460250033 1107 54-55 0.37436725347910027 1107 56-57 0.6815562384308329 1107 58-59 0.5685217668980336 1107 60-61 0.6086619095777195 1107 62-63 0.3798681102509036 1107 64-65 0.21679980863786952 1107 66-67 0.4317416471182902 1107 68-69 -2.6053811276822607 1107 70-71 -2.4763479943271314 1107 72-73 1.1199886700884143 1107 74-75 1.4501511430071474 1107 76-77 1.1781309259230106 1107 78-79 0.936663166682667 1107 80-81 0.6418997305147087 1107 82-83 0.6217116649347219 1107 84-85 0.5004300925630254 1107 86-87 0.7511569551922648 1107 88-89 0.6345683490565932 1107 90-91 0.6298703248528454 1107 92-93 0.5473052857480454 1107 94-95 0.8776283359726627 1107 96-97 0.7958877041021175 1107 98-99 0.8809923614275128 1107 100-101 1.2088584621029597 1108 1 -0.07767558226797533 1108 2 -0.06031691143578044 1108 3 -0.1253642967443227 1108 4 0.04360136524484659 1108 5 -0.10535910314437302 1108 6 -0.24796709569584863 1108 7 0.08676049742610559 1108 8 0.2095832155581263 1108 9 0.1590053513058507 1108 10-11 0.1918434919424854 1108 12-13 0.01648128914598601 1108 14-15 -0.01895282349379812 1108 16-17 0.25134384487457595 1108 18-19 0.25145000434041975 1108 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62-63 1.4012848122565877 2311 64-65 1.3214066031532532 2311 66-67 1.6002066771957217 2311 68-69 4.110003439375486 2311 70-71 5.45057578333547 2311 72-73 4.170123586609801 2311 74-75 3.0625933048127365 2311 76-77 2.6845579155097816 2311 78-79 2.0445294752472734 2311 80-81 1.7002424090552886 2311 82-83 1.5442365985758997 2311 84-85 1.5721781960356758 2311 86-87 1.507174954532605 2311 88-89 1.490339289388622 2311 90-91 1.7338111751881726 2311 92-93 1.7841989074477738 2311 94-95 2.2185708668746713 2311 96-97 2.0185191259871793 2311 98-99 2.3584399418826827 2311 100-101 2.5144871039851893 2312 1 -0.18999120857953145 2312 2 -0.11827407644553745 2312 3 -0.10793836006948254 2312 4 0.03058331016192284 2312 5 -1.3654741079931725 2312 6 -0.2511678009054634 2312 7 -0.07893257854129132 2312 8 0.07764205182402151 2312 9 -2.648606578940253 2312 10-11 -0.21496763510536 2312 12-13 -1.2598670231941114 2312 14-15 -4.23530085292704 2312 16-17 -1.0768888601537228 2312 18-19 -0.14154907990940302 2312 20-21 0.10523480116237494 2312 22-23 0.2918426398906533 2312 24-25 0.3600824920729906 2312 26-27 0.27607054113629914 2312 28-29 0.2344042253348988 2312 30-31 0.2977126963790724 2312 32-33 0.610739581740134 2312 34-35 0.4680306995328394 2312 36-37 0.5628279329741943 2312 38-39 0.5449855520204565 2312 40-41 0.31841862933409004 2312 42-43 -0.030963566039652335 2312 44-45 0.6541439042027832 2312 46-47 0.6552932449114692 2312 48-49 0.7536601465777082 2312 50-51 0.9201624114821882 2312 52-53 0.9919615211901771 2312 54-55 0.9572219169693668 2312 56-57 0.8725575400984376 2312 58-59 0.7154378789233142 2312 60-61 0.8823595461095515 2312 62-63 0.9057628634661867 2312 64-65 1.0774791317707155 2312 66-67 1.4998356455300907 2312 68-69 4.103841719449566 2312 70-71 4.985441020908116 2312 72-73 2.9940720586812226 2312 74-75 2.246543067756022 2312 76-77 2.196086570985269 2312 78-79 1.617692875813166 2312 80-81 1.5019784854982987 2312 82-83 1.624940946725296 2312 84-85 1.3494327204580117 2312 86-87 1.2930898087914997 2312 88-89 1.433549282491441 2312 90-91 1.6965254484294015 2312 92-93 1.878023986372682 2312 94-95 1.9571723429803818 2312 96-97 1.7831995558266236 2312 98-99 2.079195323335316 2312 100-101 1.88940778220676 2313 1 -0.32364193017393816 2313 2 -0.157991356163123 2313 3 -0.09477640540752574 2313 4 -0.010480618909475936 2313 5 0.059956980417361194 2313 6 0.02591312316819483 2313 7 -2.6740797378128 2313 8 -2.650123228799785 2313 9 -0.9172217026663034 2313 10-11 -0.11296074293198188 2313 12-13 -0.11587624516385375 2313 14-15 -0.6660259724601403 2313 16-17 -0.3179346563760035 2313 18-19 -0.009453493165906934 2313 20-21 -2.650958369896756 2313 22-23 -0.8318646020903131 2313 24-25 -0.16514404173780406 2313 26-27 -1.7498063871167986 2313 28-29 -1.8768993447652633 2313 30-31 -1.193394916374288 2313 32-33 -1.1948796571705103 2313 34-35 -1.7694036375741575 2313 36-37 -2.4364680982565687 2313 38-39 -2.0065117394644574 2313 40-41 -1.113819040328508 2313 42-43 -0.3351612360742138 2313 44-45 -0.5042482235201078 2313 46-47 -0.5848013164172912 2313 48-49 -2.325577656650868 2313 50-51 -0.9236967448880584 2313 52-53 -0.7137979197263249 2313 54-55 -0.5585784940418783 2313 56-57 -0.654863956077179 2313 58-59 -0.4319573520950968 2313 60-61 -0.26426715220717867 2313 62-63 -0.14651585237304943 2313 64-65 -0.22459321195709592 2313 66-67 0.36478417190852497 2313 68-69 1.2046651156434436 2313 70-71 3.7141907165805748 2313 72-73 2.7304201155498937 2313 74-75 1.4897354298111125 2313 76-77 1.5602962577307906 2313 78-79 0.8411830942188949 2313 80-81 0.631255661102049 2313 82-83 0.6315424238973506 2313 84-85 0.3960856036232876 2313 86-87 0.46459803070714045 2313 88-89 0.10479956568511994 2313 90-91 0.701117941176264 2313 92-93 0.6597251942148645 2313 94-95 1.0620715091229265 2313 96-97 1.1618240122638248 2313 98-99 1.3085018961338442 2313 100-101 1.3347776077932707 2314 1 -0.13304243923963455 2314 2 -0.17400221028130147 2314 3 -0.11955495496505364 2314 4 -0.040798004035011104 2314 5 -0.248834228845368 2314 6 -2.52051466364896 2314 7 -0.751200787662107 2314 8 -0.1415760827257344 2314 9 -0.16496943881718096 2314 10-11 -0.28914246273645716 2314 12-13 -2.1916078125575567 2314 14-15 -0.6741062747414901 2314 16-17 -1.5951388198674508 2314 18-19 -0.20607078509431176 2314 20-21 -0.7390818938465245 2314 22-23 -0.6442674144809004 2314 24-25 -0.2989897232610872 2314 26-27 -1.8213032136088074 2314 28-29 -0.823922014150611 2314 30-31 -0.5556546505822553 2314 32-33 -0.2186097390741324 2314 34-35 -0.3085450354623802 2314 36-37 -0.5789075887789537 2314 38-39 -0.4188806970983805 2314 40-41 -0.4420981888878188 2314 42-43 -0.668602376855084 2314 44-45 -3.6109703021420927 2314 46-47 -3.9560275193982193 2314 48-49 -4.111648848612916 2314 50-51 -4.135613469052032 2314 52-53 -1.2509117867033055 2314 54-55 -1.6678380703468108 2314 56-57 -1.3147288846088863 2314 58-59 -1.8613514120227386 2314 60-61 -1.2652368828795026 2314 62-63 -1.3404324872874724 2314 64-65 -1.4671972992431535 2314 66-67 -1.2153471193264807 2314 68-69 -1.3944221575425608 2314 70-71 1.4558885776808168 2314 72-73 1.1562051468558892 2314 74-75 0.666726724547452 2314 76-77 0.6471079634956247 2314 78-79 -1.694841649360285 2314 80-81 -1.02001771112349 2314 82-83 0.10591653998355 2314 84-85 0.12700218361490911 2314 86-87 -0.4261907722504503 2314 88-89 -0.33773927932274006 2314 90-91 0.022182357896134874 2314 92-93 -0.20915220093407783 2314 94-95 0.34847245242200486 2314 96-97 0.5536977550466275 2314 98-99 0.36194950321613817 2314 100-101 0.3970669273684422 2315 1 -0.3295739696634996 2315 2 -0.29360541739891133 2315 3 -0.37662080703139367 2315 4 -0.08442772958528622 2315 5 -0.2079553568762833 2315 6 -0.048834211783329806 2315 7 -0.950366484253486 2315 8 -2.7284720165753384 2315 9 -0.6472329519388254 2315 10-11 -1.2338582627690258 2315 12-13 -0.42554995415900976 2315 14-15 -0.3479683172769583 2315 16-17 -0.09974623283557804 2315 18-19 -0.028864476643313708 2315 20-21 -0.5628159282013385 2315 22-23 -0.2438448111777447 2315 24-25 -4.9765428461920855 2315 26-27 -0.6989180671974182 2315 28-29 -0.7407778242326799 2315 30-31 -0.7882141739222988 2315 32-33 -0.008099149285371254 2315 34-35 -2.128083542540871 2315 36-37 -3.5291632571658553 2315 38-39 -1.0002731839136345 2315 40-41 -0.08679150977449979 2315 42-43 -1.402300008448556 2315 44-45 -4.879772982565655 2315 46-47 -3.2814692960905276 2315 48-49 -2.453266215040628 2315 50-51 -0.9322346144816649 2315 52-53 0.12713443914321232 2315 54-55 -0.783498959555935 2315 56-57 -0.7681061152987425 2315 58-59 -0.14574786899384762 2315 60-61 -1.1324341950324843 2315 62-63 -0.014077625783603054 2315 64-65 0.6064028809611877 2315 66-67 0.7663508194123949 2315 68-69 3.5679724816103118 2315 70-71 4.072267570049043 2315 72-73 -1.989337927502529 2315 74-75 -1.601839681262149 2315 76-77 0.17129809948865926 2315 78-79 0.2866403132709756 2315 80-81 0.060655164643620196 2315 82-83 -0.5235686759763212 2315 84-85 -0.09924102450485961 2315 86-87 0.233030247630019 2315 88-89 0.21549245167356545 2315 90-91 0.3471457857872551 2315 92-93 -0.8334249924449857 2315 94-95 0.2532896243552223 2315 96-97 0.049278944200406016 2315 98-99 -2.0083673993848983 2315 100-101 -3.637801846847676 2316 1 -0.4618872996167127 2316 2 -0.3721462794695185 2316 3 -0.8873050402836355 2316 4 -0.3392056573119362 2316 5 -0.06543747961069357 2316 6 -0.5298789692648498 2316 7 -0.5872540070529908 2316 8 -0.8166264067423015 2316 9 -5.058552486966935 2316 10-11 -1.2678955283234714 2316 12-13 -0.4609345028053795 2316 14-15 -0.23287616391586852 2316 16-17 -0.3330152737094281 2316 18-19 -0.4048461026407395 2316 20-21 -3.8611627795007095 2316 22-23 -1.1544320290143375 2316 24-25 -0.4575902365551414 2316 26-27 -0.7942481879137873 2316 28-29 -0.2027608955566933 2316 30-31 0.052031225681417936 2316 32-33 -1.280032933067126 2316 34-35 -0.5911212709809561 2316 36-37 -0.40308509217896216 2316 38-39 -5.449191897081505 2316 40-41 -4.961034305583752 2316 42-43 -3.6240983367443107 2316 44-45 -2.466450326193808 2316 46-47 -2.322043630360163 2316 48-49 -2.877464803515224 2316 50-51 -1.4315584654294788 2316 52-53 -0.9597482692443471 2316 54-55 -0.19141656089554715 2316 56-57 -3.587099271311267 2316 58-59 -0.8754066024123368 2316 60-61 -0.515369135777604 2316 62-63 -0.09861330834936055 2316 64-65 0.08742745301763577 2316 66-67 -0.011887587257973564 2316 68-69 2.6811001292234415 2316 70-71 3.83710849636196 2316 72-73 2.460709288839592 2316 74-75 0.9377947143009706 2316 76-77 -1.6137385326697071 2316 78-79 -0.030628179963517965 2316 80-81 -0.1469983210658512 2316 82-83 0.048160043946772646 2316 84-85 0.5004877636223988 2316 86-87 0.40131676331472477 2316 88-89 0.277809247000377 2316 90-91 0.2991356925732198 2316 92-93 0.0849892806816186 2316 94-95 0.42572330457696594 2316 96-97 0.4771179302050079 2316 98-99 0.6327327338639819 2316 100-101 0.685074809422872 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 24.0 4 88.0 5 182.0 6 499.0 7 1174.0 8 2709.0 9 4464.0 10 7106.0 11 10547.0 12 13893.0 13 17824.0 14 22235.0 15 27949.0 16 33498.0 17 37877.0 18 34380.0 19 29849.0 20 29692.0 21 30670.0 22 31668.0 23 35610.0 24 42424.0 25 51793.0 26 65657.0 27 81684.0 28 99745.0 29 118699.0 30 139887.0 31 161820.0 32 186466.0 33 214478.0 34 245611.0 35 272783.0 36 278549.0 37 227737.0 38 94973.0 39 5779.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.20382869248257 21.18208022717069 15.928494776081802 21.68559630426494 2 15.607861421207186 22.066687472034953 39.004876728192905 23.320574378564956 3 19.494431612441428 25.999956391239753 30.916731327931863 23.588880668386956 4 12.826522265558275 18.154987880872095 37.78884046923337 31.229649384336263 5 12.878195780425115 38.778119503118575 34.12918871998109 14.214495996475229 6 30.204760771584994 37.70779363097648 17.32938450717304 14.758061090265489 7 27.174683302563015 34.09847479221107 20.998326711230057 17.728515193995857 8 26.18879253662833 34.6553104080366 19.527779939406724 19.628117115928347 9 25.846277731349748 17.496675790620486 21.24572984299807 35.411316635031696 10-11 24.710529590705367 27.479865747840698 26.988376066962154 20.82122859449178 12-13 26.06074891608406 25.114293780786767 27.6645460146893 21.16041128843987 14-15 22.690036319125603 26.189769968846228 27.22966386481962 23.890529847208548 16-17 21.11290516612657 29.672483665536348 27.556164991240667 21.658446177096412 18-19 20.754311608749983 28.05374605079345 30.49659627643017 20.695346064026392 20-21 22.226658260749595 26.501025739925588 30.2339521607079 21.03836383861692 22-23 21.584759541733632 26.79256773902501 30.339169744645254 21.283502974596104 24-25 21.640324006821196 27.008275362188076 30.51887558535067 20.832525045640057 26-27 21.506712377022463 26.88839998842078 30.78027787876179 20.824609755794967 28-29 20.79600721954616 27.56188813462852 30.657338098745075 20.984766547080245 30-31 21.18054087055148 26.917551385296353 30.56001509759395 21.341892646558218 32-33 21.20924927462788 27.110214457517895 30.453693100863376 21.22684316699085 34-35 21.923464652952553 26.899609975411288 30.257723751912856 20.919201619723303 36-37 21.626659869007092 26.551300851933618 30.66737151623866 21.15466776282063 38-39 21.15503764975092 27.297607541352072 30.571153711192423 20.97620109770459 40-41 21.37313007191803 27.140718819137938 30.487632133934472 20.99851897500956 42-43 21.174687422929196 27.232464258430028 29.902314433260237 21.690533885380535 44-45 21.51222529441576 27.107225239861226 29.72025564630002 21.660293819422993 46-47 21.890345669313856 27.50700172616441 29.295094352116468 21.30755825240527 48-49 21.795321322814882 27.532759807062224 29.140399065633982 21.531519804488912 50-51 21.19457648543766 27.768782060566842 29.356540778877786 21.680100675117707 52-53 21.80635526955495 28.110035630961416 28.519269776876264 21.56433932260737 54-55 21.38943744996424 27.60661272223306 28.880099792249926 22.123850035552774 56-57 21.80323119048125 27.787779371138665 28.60120817576515 21.807781262614935 58-59 22.331693149506403 27.42468578183976 28.559522278599626 21.684098790054215 60-61 21.75955299810071 27.742594914304398 29.23078398097813 21.267068106616765 62-63 21.517695571027307 27.677963709036952 29.1813245507897 21.623016169146045 64-65 21.359876669806567 28.033237945802615 28.79145696667652 21.815428417714298 66-67 21.367331812000632 27.836491313022076 28.5049752700284 22.291201604948892 68-69 21.376349429020717 27.36472002472375 29.258901005008376 22.00002954124716 70-71 21.234119644514482 27.38119738596408 29.638006517828806 21.746676451692633 72-73 21.664033620484407 27.263592762902462 29.164083615032173 21.90829000158096 74-75 21.235456609428436 28.320963395717136 28.444882185935732 21.998697808918692 76-77 20.801736273431047 27.945029308937407 28.876601858398715 22.376632559232835 78-79 21.498788843258364 27.88811299241723 28.427206988896085 22.185891175428324 80-81 21.520187041180115 28.06085755779528 28.233454851953926 22.185500549070678 82-83 21.45662338638809 27.949586229258593 28.459926973341037 22.13386341101227 84-85 21.259749423784477 28.05247611962125 28.23053808811859 22.457236368475684 86-87 21.296601052900478 28.028968578964058 28.289364735258893 22.385065632876568 88-89 21.26799160383989 28.04661083136233 27.845522533524587 22.839875031273195 90-91 21.280346055325317 27.84476910314834 28.161301000800208 22.713583840726127 92-93 21.52749359520091 28.06333048800913 28.127524160035673 22.281651756754293 94-95 21.243282859388422 27.64363666735768 28.253376018035027 22.859704455218875 96-97 20.916633462258574 27.700467970611136 28.478043440203578 22.90485512692671 98-99 21.039499820091343 27.861703819590527 27.743663436582477 23.355132923735646 100-101 20.818496038813645 27.46534079437286 28.260510745077518 23.455652421735973 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1590.0 1 1126.0 2 647.5 3 665.0 4 659.5 5 722.0 6 1182.5 7 2289.0 8 3443.5 9 4121.5 10 4167.0 11 3897.0 12 3813.5 13 3866.0 14 4112.5 15 4394.5 16 4649.0 17 4950.0 18 5273.0 19 5644.5 20 6258.5 21 7250.5 22 8448.5 23 9804.5 24 11429.0 25 13634.5 26 17264.5 27 21473.5 28 24923.5 29 28863.5 30 33944.5 31 39248.0 32 46238.5 33 54115.0 34 62027.0 35 68977.5 36 75999.0 37 82816.5 38 85210.5 39 85512.5 40 85397.0 41 86540.0 42 88680.5 43 90229.0 44 92214.5 45 94135.0 46 95963.5 47 104106.0 48 118051.0 49 116245.5 50 100321.5 51 88776.5 52 81433.5 53 77052.5 54 73202.5 55 66924.0 56 60457.5 57 54421.0 58 48504.0 59 42576.5 60 36066.5 61 28999.0 62 22669.5 63 17554.0 64 13125.5 65 9718.0 66 6962.0 67 5067.5 68 3464.0 69 2099.5 70 1418.5 71 1035.0 72 790.5 73 624.5 74 455.5 75 333.0 76 232.5 77 135.5 78 74.5 79 41.0 80 22.5 81 16.5 82 12.5 83 8.0 84 5.0 85 4.0 86 2.5 87 2.0 88 1.5 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020037360466851704 2 0.01733062509421883 3 5.63903202631849E-4 4 0.08454788684793524 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.00977432217895205 12-13 0.0 14-15 0.0 16-17 4.1352901526335595E-4 18-19 1.1278064052636982E-4 20-21 0.0 22-23 2.2556128105273964E-4 24-25 0.003270638575264724 26-27 0.0035337934031595872 28-29 0.001127806405263698 30-31 1.691709607895547E-4 32-33 6.390902963160956E-4 34-35 0.0026503450523696903 36-37 0.08471705780872478 38-39 0.0968597734387306 40-41 0.07798781292398473 42-43 0.08144641923346006 44-45 0.0804501902421438 46-47 0.07951035157109071 48-49 0.07413447437266708 50-51 0.07428484856003557 52-53 0.05746173634818541 54-55 0.04915356249607617 56-57 0.027537273061855293 58-59 0.021541102340536633 60-61 0.013364505902374821 62-63 0.002161628943422088 64-65 0.006748041658161127 66-67 0.15960340311823434 68-69 0.7389011631819329 70-71 0.9033917273896432 72-73 0.3662363333359649 74-75 0.053946739718446886 76-77 0.20225328201062318 78-79 0.014417125213954274 80-81 0.0030826708410541075 82-83 0.001635319287632362 84-85 7.518709368424654E-5 86-87 0.0017105063813166088 88-89 2.0676450763167797E-4 90-91 0.004116493379212498 92-93 0.004191680472896745 94-95 0.07864569999372188 96-97 0.03746196942817583 98-99 0.013082554301058896 100-101 0.013082554301058896 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2660031.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.22103033657162 #Duplication Level Percentage of deduplicated Percentage of total 1 72.22205716599431 34.10399952405028 2 14.489814553656737 13.684479452190438 3 5.421280855907368 7.679954032796305 4 2.6009233353066317 4.91273118878446 5 1.4280155964376124 3.3716183900238965 6 0.8869524439899437 2.5129684958847287 7 0.5851447257214895 1.9341795791205534 8 0.4056870078030785 1.532556680209771 9 0.28350734473037364 1.2048758033538458 >10 1.4622218574857035 12.739203209794262 >50 0.1275830815710762 4.216122270449832 >100 0.0798576707720953 6.814853064862356 >500 0.0046092855295509 1.429231039164874 >1k 0.0019407518019131848 1.930754469347438 >5k 1.6172931682609872E-4 0.5519659801518347 >10k+ 2.425939752391481E-4 1.380506819815239 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13892 0.5222495527307764 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11907 0.44762636224916175 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10354 0.3892435840033443 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8069 0.3033423294690927 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6386 0.24007239013379922 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4513 0.1696596768985023 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4021 0.15116365185217764 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3397 0.12770527862269274 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3129 0.1176302080690037 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2996 0.1126302663390013 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2788 0.10481080859583967 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2781 0.10454765376794481 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.759354684212327E-5 0.0 10-11 0.0 0.0 0.0 7.518709368424654E-5 0.0 12-13 0.0 0.0 0.0 9.398386710530817E-5 0.0 14-15 0.0 0.0 0.0 1.127806405263698E-4 0.0 16-17 0.0 0.0 0.0 1.127806405263698E-4 0.0 18-19 0.0 0.0 0.0 1.3157741394743144E-4 0.0 20-21 0.0 0.0 0.0 1.8796773421061633E-4 0.0 22-23 0.0 0.0 0.0 3.7593546842123267E-4 0.0 24-25 0.0 0.0 0.0 4.887161089476025E-4 0.0 26-27 0.0 0.0 0.0 0.0010150257647373283 0.0 28-29 0.0 0.0 0.0 0.0022368160371063345 0.0 30-31 0.0 0.0 0.0 0.0067856352050032495 0.0 32-33 0.0 0.0 0.0 0.016954689625797596 0.0 34-35 0.0 0.0 0.0 0.033420663142647584 0.0 36-37 0.0 0.0 0.0 0.05849555888634381 0.0 38-39 0.0 0.0 0.0 0.09652143151715148 0.0 40-41 0.0 0.0 0.0 0.13941566846401415 0.0 42-43 0.0 0.0 0.0 0.18308057312114032 0.0 44-45 0.0 0.0 0.0 0.23494087098984937 0.0 46-47 0.0 0.0 0.0 0.3027784262664608 0.0 48-49 0.0 0.0 0.0 0.3745633039614952 0.0 50-51 0.0 0.0 0.0 0.452569913658901 0.0 52-53 0.0 0.0 0.0 0.5275690396089369 0.0 54-55 0.0 0.0 0.0 0.6142033683066099 0.0 56-57 0.0 1.8796773421061635E-5 0.0 0.7160818802487641 0.0 58-59 0.0 3.759354684212327E-5 0.0 0.8193137598772345 0.0 60-61 0.0 3.759354684212327E-5 0.0 0.9247072684491271 0.0 62-63 1.8796773421061635E-5 3.759354684212327E-5 0.0 1.0297060447791773 0.0 64-65 3.759354684212327E-5 3.759354684212327E-5 0.0 1.1450242497173906 0.0 66-67 3.759354684212327E-5 3.759354684212327E-5 0.0 1.265605551213501 0.0 68-69 3.759354684212327E-5 3.759354684212327E-5 0.0 1.4070136776601476 0.0 70-71 3.759354684212327E-5 3.759354684212327E-5 0.0 1.5828950865610212 0.0 72-73 3.759354684212327E-5 3.759354684212327E-5 0.0 1.77373872710506 0.0 74-75 3.759354684212327E-5 3.759354684212327E-5 0.0 1.980446844416475 0.0 76-77 3.759354684212327E-5 3.759354684212327E-5 0.0 2.2136208187047446 0.0 78-79 3.759354684212327E-5 3.759354684212327E-5 0.0 2.492564936273299 0.0 80-81 3.759354684212327E-5 3.759354684212327E-5 0.0 2.7939711980800226 0.0 82-83 3.759354684212327E-5 3.759354684212327E-5 0.0 3.0968060146667464 0.0 84-85 3.759354684212327E-5 3.759354684212327E-5 0.0 3.4054114406937366 0.0 86-87 3.759354684212327E-5 3.759354684212327E-5 0.0 3.727663324224417 0.0 88-89 3.759354684212327E-5 3.759354684212327E-5 0.0 4.082696780601429 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGCATA 400 0.0 24.602161 64-65 CGCATAG 410 0.0 24.590332 66-67 GTACATG 15135 0.0 24.049803 1 TACATGG 15425 0.0 23.227146 2 CGCGGAA 300 0.0 22.124031 82-83 ACATGGG 15485 0.0 22.089804 3 GAGTACT 12755 0.0 21.59378 12-13 CGATCGT 330 0.0 21.549583 24-25 TCGCGGA 315 0.0 21.070507 82-83 ATTCGCG 315 0.0 21.07031 80-81 GTACTTT 13350 0.0 20.613605 14-15 AGTACTT 13270 0.0 20.202015 12-13 CTCGCGC 330 0.0 19.422192 60-61 CGCGCTT 300 0.0 19.164297 62-63 GTATCAA 29240 0.0 19.151518 1 TCGCGCT 335 0.0 19.132309 60-61 CATGGGA 10285 0.0 19.008265 4 AGAGTAC 22425 0.0 18.816177 10-11 GGTATCA 23480 0.0 18.715933 1 AACGCAT 530 0.0 18.567669 64-65 >>END_MODULE