FastQCFastQC Report
Fri 27 May 2016
SRR939443_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939443_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2445050
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74580.3050244371280751No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62780.25676366536471645No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36370.14874951432486044No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA96850.031.1770761
GTATCAA141700.029.9174161
GTACATG103550.025.5389861
TATCAAC167800.025.2691612
TACATGG102700.025.0382062
ATCAACG169650.024.9895083
TCAACGC171700.024.7537144
CAACGCA172100.024.2991875
AACGCAG176450.023.6446296
ACATGGG107700.023.6182173
GAGTACT87500.021.83972712-13
CGCAGAG193900.021.3655958
ACGCAGA193450.021.202697
CATGGGG44800.020.8331184
GTACTTT94950.020.1831814-15
GCAGAGT194600.019.7021489
AGAGTAC147750.019.56271610-11
AGTACTT93000.019.26065412-13
CATGGGA68800.019.131194
GTCTAGG8500.018.2322751
Produced by FastQC (version 0.11.5)