FastQCFastQC Report
Fri 27 May 2016
SRR939443_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939443_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2445050
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101390.41467454653279073No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98900.4044907057115396No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59070.24159015153064353No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29960.12253328152798512No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT29700.12146990859082636No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA107900.032.50161
GTATCAA151850.028.0568791
ATCAACG183600.022.965173
TATCAAC185750.022.8806972
TCAACGC185200.022.8179284
CAACGCA188300.022.3667955
AACGCAG191300.022.04086
GTACATG120300.021.9030021
TACATGG121350.021.2014542
GAGTACT100750.020.9725412-13
ACATGGG124150.019.9957143
GTACTTT107000.019.79178414-15
ACGCAGA216250.019.3882887
CGCAGAG216700.019.282448
AGTACTT103800.019.14678412-13
AGAGTAC177750.018.48371110-11
GTACCGT1801.2591493E-418.4237426
GCAGAGT223100.018.0922419
ACTTTTT122750.017.77335216-17
GTATAGC10300.017.4840011
Produced by FastQC (version 0.11.5)