##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939443_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2445050 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.947031758041756 31.0 31.0 31.0 30.0 34.0 2 31.204358193083987 31.0 31.0 34.0 30.0 34.0 3 31.19152328173248 31.0 31.0 34.0 28.0 34.0 4 35.133049630886894 35.0 35.0 37.0 33.0 37.0 5 34.74840228216192 35.0 35.0 37.0 32.0 37.0 6 34.7048788368336 35.0 35.0 37.0 31.0 37.0 7 34.573961268685714 35.0 35.0 37.0 30.0 37.0 8 34.589737633177236 35.0 35.0 37.0 30.0 37.0 9 35.93863029385902 37.0 35.0 39.0 30.0 39.0 10-11 35.78529477924786 37.0 35.0 39.0 30.0 39.0 12-13 35.75678718226621 37.0 35.0 39.0 30.0 39.0 14-15 36.61153064354512 38.0 35.0 40.0 30.0 41.0 16-17 36.615256538721084 38.0 35.0 40.0 30.5 41.0 18-19 36.56893355964091 38.0 35.0 40.0 30.0 41.0 20-21 36.470422077258135 38.0 35.0 40.0 30.0 41.0 22-23 36.33067790024744 38.0 34.0 40.0 30.0 41.0 24-25 36.11645078832744 38.0 34.0 40.0 29.0 41.0 26-27 35.746028506574504 38.0 34.0 40.0 27.5 41.0 28-29 35.46720251119609 38.0 34.0 40.0 27.0 41.0 30-31 35.25916422977035 38.0 34.0 40.0 27.0 41.0 32-33 35.06482239627002 37.5 33.0 40.0 27.0 41.0 34-35 35.03924868612094 38.0 33.0 40.0 26.5 41.0 36-37 35.04829962577452 38.0 33.0 40.0 26.0 41.0 38-39 35.02759268726611 38.0 33.0 40.0 26.0 41.0 40-41 34.82495020551727 38.0 33.0 40.0 25.5 41.0 42-43 34.531460501830225 38.0 33.0 40.0 24.5 41.0 44-45 34.353175599680995 37.0 33.0 40.0 24.0 41.0 46-47 34.04924480071982 37.0 33.0 40.0 24.0 41.0 48-49 33.74571931044355 37.0 32.5 40.0 23.0 41.0 50-51 33.48800065438334 37.0 32.0 40.0 22.5 41.0 52-53 33.123546144250625 37.0 31.0 40.0 21.0 41.0 54-55 32.904435492116725 36.5 31.0 39.0 20.5 40.5 56-57 32.51639496124824 36.0 31.0 39.0 19.0 40.0 58-59 32.08417189832519 36.0 30.0 39.0 17.5 40.0 60-61 31.792150058281017 35.0 30.0 39.0 16.0 40.0 62-63 31.40872006707429 35.0 29.0 38.0 13.0 40.0 64-65 30.981049058301465 34.5 29.0 38.0 10.0 40.0 66-67 29.260855401730026 33.5 25.5 37.5 7.0 39.5 68-69 25.542473160058076 30.0 16.0 35.0 4.5 39.0 70-71 24.903410768695935 29.5 15.0 35.0 2.0 38.0 72-73 26.76653033680293 31.0 21.5 35.0 2.0 38.0 74-75 27.3916433201775 32.0 24.5 35.0 2.0 38.0 76-77 27.47469642747592 31.5 25.5 34.5 2.0 36.5 78-79 28.316104987628066 33.0 26.0 35.0 2.0 37.0 80-81 28.169430890983826 33.0 26.0 35.0 2.0 37.0 82-83 27.757192490951105 33.0 26.0 35.0 2.0 36.0 84-85 27.413174372712213 32.0 26.0 35.0 2.0 36.0 86-87 26.964387435839758 32.0 25.5 34.5 2.0 35.5 88-89 26.469941923478046 32.0 25.0 34.0 2.0 35.0 90-91 26.012614670456635 32.0 24.0 34.0 2.0 35.0 92-93 25.434674137543198 31.5 21.5 34.0 2.0 35.0 94-95 24.034891311016136 31.0 13.5 34.0 2.0 35.0 96-97 23.22217684709924 30.0 4.5 34.0 2.0 35.0 98-99 22.878852170712257 30.0 2.0 34.0 2.0 35.0 100-101 21.60842130017791 29.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3460625258883816 1101 2 0.16514669889761535 1101 3 0.3297149227276428 1101 4 0.234839357071273 1101 5 0.3765995491647871 1101 6 0.4987292637678564 1101 7 0.5588022204984995 1101 8 0.43706439082173887 1101 9 0.5790831583954201 1101 10-11 0.7147178504973226 1101 12-13 0.5761206823018057 1101 14-15 0.7024079730973298 1101 16-17 0.6538257168360033 1101 18-19 0.8385565163637523 1101 20-21 0.7368126019013062 1101 22-23 0.7603723959913324 1101 24-25 0.6927013958374104 1101 26-27 0.7031302979691034 1101 28-29 0.6514243481361888 1101 30-31 0.6429207499668479 1101 32-33 0.5355510329863833 1101 34-35 0.6858044973474975 1101 36-37 0.5716019681834368 1101 38-39 0.6537577270684167 1101 40-41 0.7165186082524215 1101 42-43 0.6805064591518715 1101 44-45 0.26150666344912565 1101 46-47 0.35492022144151747 1101 48-49 0.4090391976774086 1101 50-51 0.35992622534151764 1101 52-53 0.5429430452998147 1101 54-55 0.4547890072800129 1101 56-57 0.25107481516830177 1101 58-59 0.383495239799462 1101 60-61 0.1131076120191139 1101 62-63 0.12458354554963691 1101 64-65 -0.0751325273220651 1101 66-67 -3.1523596618720653 1101 68-69 -7.889874835923134 1101 70-71 -11.309385324901296 1101 72-73 -11.246776062319299 1101 74-75 -9.851214716178006 1101 76-77 -4.769079475361213 1101 78-79 -1.4937241211535301 1101 80-81 -0.8391241873059165 1101 82-83 -0.5396255575476481 1101 84-85 -0.6968976919964724 1101 86-87 -0.6215107109808535 1101 88-89 -0.4443920137191526 1101 90-91 -0.5365020913379439 1101 92-93 -1.1557660148885027 1101 94-95 -4.965715682914318 1101 96-97 -1.8521417043356685 1101 98-99 -1.1972512193098446 1101 100-101 -1.0151519644055078 1102 1 0.24533584952665777 1102 2 0.19135440393758074 1102 3 0.3379289399490304 1102 4 0.23475487841252374 1102 5 0.2741056165712905 1102 6 0.38388225941573495 1102 7 0.36166380847875956 1102 8 0.4122090105571843 1102 9 0.5448690351093433 1102 10-11 0.55254054498279 1102 12-13 0.44487049409028145 1102 14-15 0.44874201237377775 1102 16-17 0.45450398452782537 1102 18-19 0.5110540093196434 1102 20-21 0.5957771467145676 1102 22-23 0.6447301729714141 1102 24-25 0.5668603734804094 1102 26-27 0.47850560840151957 1102 28-29 0.7252630930338171 1102 30-31 0.3482633189228821 1102 32-33 0.25223426226705925 1102 34-35 0.42020605932324884 1102 36-37 0.6371863886132019 1102 38-39 0.5179887300965973 1102 40-41 0.6029356524747342 1102 42-43 0.7683266219425136 1102 44-45 0.3199848163885406 1102 46-47 0.4643148878800645 1102 48-49 0.13618059176715747 1102 50-51 0.28551605027347904 1102 52-53 0.4540026741746672 1102 54-55 0.3823280373530835 1102 56-57 0.37682769457142484 1102 58-59 0.17766378724888554 1102 60-61 0.012781609674497929 1102 62-63 -0.06982236827626664 1102 64-65 -0.1592361621162901 1102 66-67 -3.2246831207961186 1102 68-69 -8.264874069752334 1102 70-71 -11.350320231829532 1102 72-73 -9.547757800271828 1102 74-75 -8.307241175853303 1102 76-77 -4.680750375288113 1102 78-79 -1.7738422953378645 1102 80-81 -1.1365884684231808 1102 82-83 -0.7050597552015923 1102 84-85 -0.8420258983546454 1102 86-87 -0.9584532417749223 1102 88-89 -0.9264144487991679 1102 90-91 -0.9084580281223253 1102 92-93 -1.168150333091095 1102 94-95 -4.46842472961929 1102 96-97 -1.3987434103781844 1102 98-99 -0.7912375247063252 1102 100-101 -0.8035245296812619 1103 1 0.32321070342733904 1103 2 0.28840728734604326 1103 3 0.35463082147904856 1103 4 0.10530792079495654 1103 5 0.3740637591336693 1103 6 0.2866715874286925 1103 7 0.3635008995422595 1103 8 0.41874669976847656 1103 9 0.5520182538451479 1103 10-11 0.6162778451754249 1103 12-13 0.5149755117407366 1103 14-15 0.6469703021536901 1103 16-17 0.6213379448446545 1103 18-19 0.5593913813205731 1103 20-21 0.6598082260595675 1103 22-23 0.6866815856796862 1103 24-25 0.8410907326878245 1103 26-27 0.8044661891793865 1103 28-29 0.8604659751647006 1103 30-31 0.7540437810089813 1103 32-33 0.7024952324587161 1103 34-35 0.7894219159426896 1103 36-37 0.8349295904674747 1103 38-39 0.7478234682926725 1103 40-41 0.9396296103312167 1103 42-43 0.9375979416424585 1103 44-45 0.7943653118236114 1103 46-47 0.7341399427570394 1103 48-49 0.7811767708600854 1103 50-51 0.7753653582296423 1103 52-53 0.8984047226339413 1103 54-55 0.952620412476854 1103 56-57 0.9769623285322169 1103 58-59 0.7690426039083 1103 60-61 0.8140775175238595 1103 62-63 0.7303043499135917 1103 64-65 0.6522022059019932 1103 66-67 -3.0013230485768894 1103 68-69 -7.453181764856328 1103 70-71 -11.50533032213254 1103 72-73 -9.22643110652734 1103 74-75 -7.626908916307688 1103 76-77 -3.8321087400189136 1103 78-79 -0.8174176923315564 1103 80-81 -0.14250474986004846 1103 82-83 0.16854955184839682 1103 84-85 0.029483622039197854 1103 86-87 0.2392516983246864 1103 88-89 0.2747466275244079 1103 90-91 0.29351872822392266 1103 92-93 0.375031975411126 1103 94-95 1.1648732966536919 1103 96-97 1.1905809937196814 1103 98-99 0.6984370699363929 1103 100-101 0.6514178089983567 1104 1 0.45340242937770725 1104 2 0.25959048947569485 1104 3 0.39538165424717775 1104 4 0.2221001720876714 1104 5 0.47350894970811197 1104 6 0.5577101449822237 1104 7 0.5199357365407735 1104 8 0.44692442872464255 1104 9 0.7840742671472753 1104 10-11 0.7287380797882435 1104 12-13 0.6223273499772901 1104 14-15 0.7447384809273174 1104 16-17 0.7340385063019781 1104 18-19 0.7326019421948899 1104 20-21 0.7676851692181828 1104 22-23 0.6054060674403061 1104 24-25 0.7331081354115057 1104 26-27 0.9706253599436181 1104 28-29 1.0188498734244007 1104 30-31 0.9593278850633808 1104 32-33 0.8169676438075228 1104 34-35 0.8758896547678248 1104 36-37 0.7331490613738012 1104 38-39 0.8097110602256095 1104 40-41 0.8765340528428496 1104 42-43 0.9815144408585539 1104 44-45 0.7959371311664114 1104 46-47 0.6655838744514497 1104 48-49 0.7653636733900129 1104 50-51 0.8289751940608099 1104 52-53 0.7698462167651883 1104 54-55 0.7455368249009382 1104 56-57 0.6860305911168538 1104 58-59 0.8031625525928412 1104 60-61 0.5381108746788392 1104 62-63 0.7206098261736003 1104 64-65 0.65002482094825 1104 66-67 -2.7308480456024817 1104 68-69 -7.498060428818462 1104 70-71 -9.086908161517705 1104 72-73 -7.981944972014478 1104 74-75 -6.897083708580613 1104 76-77 -3.5356868136633963 1104 78-79 -0.5413385364248597 1104 80-81 0.1849930730847511 1104 82-83 0.484074362627279 1104 84-85 0.3663702840576093 1104 86-87 0.5094146193484299 1104 88-89 0.614140101661306 1104 90-91 0.46661605057133926 1104 92-93 0.5515107571360502 1104 94-95 1.4836270212581262 1104 96-97 1.4729633212812132 1104 98-99 1.2384849928503066 1104 100-101 1.1361117457710286 1105 1 0.44370179712121427 1105 2 0.40793784890977136 1105 3 0.5025375229801874 1105 4 0.4086815411767404 1105 5 0.5004162436739321 1105 6 0.587126399521523 1105 7 0.5981754117175271 1105 8 0.6611791175340542 1105 9 0.9093279369496514 1105 10-11 0.9240874339581069 1105 12-13 0.8749826698628809 1105 14-15 1.1052122208405208 1105 16-17 0.9944416239126994 1105 18-19 0.9098446429176406 1105 20-21 1.0514487281101026 1105 22-23 1.135326710791773 1105 24-25 1.0818404749179038 1105 26-27 1.1549792740716214 1105 28-29 1.238726215543224 1105 30-31 1.0771081808508285 1105 32-33 1.180319783525583 1105 34-35 1.3091564969082938 1105 36-37 1.1680020528873243 1105 38-39 1.1427030852168585 1105 40-41 1.0841225077685053 1105 42-43 1.223161533934423 1105 44-45 1.0763721960248347 1105 46-47 1.1108102789303587 1105 48-49 1.0080407897968087 1105 50-51 0.9743141220968852 1105 52-53 1.2597265719619273 1105 54-55 1.1089920456398943 1105 56-57 1.2453106370705385 1105 58-59 1.5266001079202063 1105 60-61 1.4828157757485947 1105 62-63 1.5452481573626962 1105 64-65 1.4189319260633475 1105 66-67 2.1672508570961178 1105 68-69 -5.211530796959348 1105 70-71 -5.259860999983449 1105 72-73 -1.4280823845234814 1105 74-75 1.3205008512988385 1105 76-77 1.5630836894270672 1105 78-79 1.435795968569849 1105 80-81 1.3836835113364678 1105 82-83 1.6701295070390678 1105 84-85 1.5921606046710615 1105 86-87 1.831762545334687 1105 88-89 1.8544738953182858 1105 90-91 1.7481633675140031 1105 92-93 1.8427646079203726 1105 94-95 2.8064637952156097 1105 96-97 2.7809296743769885 1105 98-99 2.4877699174477215 1105 100-101 2.580671271126203 1106 1 -0.015492582047528458 1106 2 0.03391283051389138 1106 3 0.04197116726322392 1106 4 0.05106089981330797 1106 5 0.19090260698661865 1106 6 0.15037974045057467 1106 7 0.2703822051377358 1106 8 0.1566596653752086 1106 9 0.2620517139203855 1106 10-11 0.25654251240763415 1106 12-13 0.27850755770736413 1106 14-15 0.2301261755985209 1106 16-17 0.177773770415115 1106 18-19 0.34179531822443465 1106 20-21 0.3473102486778785 1106 22-23 0.2851842910808884 1106 24-25 0.30681139756026 1106 26-27 0.3286529905139801 1106 28-29 0.42348611964728633 1106 30-31 0.3648885696566353 1106 32-33 0.3592440399760122 1106 34-35 0.4743158883013905 1106 36-37 0.28077116679936154 1106 38-39 0.2782688475183548 1106 40-41 0.336714546506677 1106 42-43 0.4362997522638423 1106 44-45 0.4313972381573308 1106 46-47 0.35851969524691185 1106 48-49 0.3451660479325369 1106 50-51 0.08727312895676675 1106 52-53 0.5507932955964847 1106 54-55 0.6126759688281993 1106 56-57 0.47910683446425395 1106 58-59 0.6924977081480854 1106 60-61 0.589655482363991 1106 62-63 0.5490400821650816 1106 64-65 0.5228365998035294 1106 66-67 1.2628880666192472 1106 68-69 -5.687442876524489 1106 70-71 -6.48896090741064 1106 72-73 -2.519983450297648 1106 74-75 0.3436737250912394 1106 76-77 0.6104762218868949 1106 78-79 0.4874456635543041 1106 80-81 0.45592116236069913 1106 82-83 0.48388131695622505 1106 84-85 0.6727725346955076 1106 86-87 0.5936047442950212 1106 88-89 0.4480222824150566 1106 90-91 0.5322586463005905 1106 92-93 0.5788917412489596 1106 94-95 1.5275917593258406 1106 96-97 1.6500162892978274 1106 98-99 1.348975975032758 1106 100-101 1.223470767909891 1107 1 -0.12255784688582949 1107 2 -0.14253296088307366 1107 3 -0.10637445077596297 1107 4 -0.08351212870965696 1107 5 0.06407580263147139 1107 6 0.03904912596584609 1107 7 0.004195702100858512 1107 8 -0.04410610207593635 1107 9 0.12634998907284967 1107 10-11 0.06872931476729605 1107 12-13 0.17237954733083427 1107 14-15 0.018476907068574633 1107 16-17 -0.10939775401087104 1107 18-19 0.012110604888356136 1107 20-21 0.02455485774985533 1107 22-23 -0.09074703928416028 1107 24-25 0.15811469884111062 1107 26-27 0.17624468929547987 1107 28-29 0.18896921414598467 1107 30-31 0.22031940738627753 1107 32-33 0.17475207803989434 1107 34-35 0.29585839837235284 1107 36-37 0.18010642078721872 1107 38-39 0.15920572782665943 1107 40-41 0.2656515764763654 1107 42-43 0.32997156977671693 1107 44-45 0.33493485928951827 1107 46-47 0.1666300954664166 1107 48-49 0.30385513886430715 1107 50-51 0.1432801630421494 1107 52-53 0.2688268696352196 1107 54-55 0.23385991514964388 1107 56-57 0.2587697221807943 1107 58-59 0.1829393339696601 1107 60-61 0.040977491318731296 1107 62-63 0.28117550078046705 1107 64-65 0.10180356205144037 1107 66-67 1.0054866872400936 1107 68-69 -5.295143578685849 1107 70-71 -6.340035351480928 1107 72-73 -2.5554744020193816 1107 74-75 -0.0072647765488014215 1107 76-77 -0.04773582336073545 1107 78-79 0.021683824714678224 1107 80-81 0.044612373495507285 1107 82-83 0.14240640394691084 1107 84-85 -0.020195874279185233 1107 86-87 0.13872141782116998 1107 88-89 -0.017326116831647198 1107 90-91 0.035077892755484896 1107 92-93 0.15467153945551715 1107 94-95 1.0042221331051628 1107 96-97 1.0969918820192959 1107 98-99 0.9429427502039331 1107 100-101 0.8185213409883048 1108 1 0.09330053694737117 1108 2 0.05013821871982316 1108 3 0.054995783788456265 1108 4 0.03993320179739612 1108 5 0.006816072324994593 1108 6 0.049791851562801526 1108 7 0.13651419459781522 1108 8 0.16453343568998946 1108 9 0.18202435109353132 1108 10-11 0.2731770071582673 1108 12-13 0.2765206079278286 1108 14-15 0.32451039510672786 1108 16-17 0.11836649065637062 1108 18-19 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2315 20-21 -1.9550518861320327 2315 22-23 -1.3821324892671214 2315 24-25 -0.8964415795495029 2315 26-27 -0.10361758524162923 2315 28-29 -0.5678404239771595 2315 30-31 -2.846132947146195 2315 32-33 -2.4229333992609057 2315 34-35 -1.651862132802627 2315 36-37 -4.982583202540777 2315 38-39 -2.117885114892651 2315 40-41 -1.6629210781172645 2315 42-43 -1.6858963520603893 2315 44-45 -1.626841187552131 2315 46-47 -1.3397869392659345 2315 48-49 -4.698442340399517 2315 50-51 -4.500049205577138 2315 52-53 -2.8764251435450383 2315 54-55 -4.460114565629951 2315 56-57 -6.343047519006781 2315 58-59 -7.384033872782926 2315 60-61 -5.854574776425178 2315 62-63 -6.964936299320584 2315 64-65 -6.242772032232601 2315 66-67 -4.301009158271441 2315 68-69 -1.988914263493502 2315 70-71 0.4992813155483624 2315 72-73 -1.2568309869500816 2315 74-75 -3.008980240625835 2315 76-77 -5.234292783488765 2315 78-79 -4.488421965460084 2315 80-81 -4.191900708852085 2315 82-83 -7.393076698876982 2315 84-85 -5.222138584161236 2315 86-87 -5.99438116431973 2315 88-89 -4.260471513049211 2315 90-91 -3.9201201132834527 2315 92-93 -4.888785352740523 2315 94-95 -2.7631429517856496 2315 96-97 -1.8444217319384322 2315 98-99 -3.4521643333379117 2315 100-101 -2.7422947132025683 2316 1 -0.39872856024471304 2316 2 -0.2674121659844779 2316 3 -0.8998820720883742 2316 4 -0.2670403495282869 2316 5 -0.11567483226752984 2316 6 -2.489918677207889 2316 7 -0.45122353319296593 2316 8 -0.5108538035648564 2316 9 -0.028834977326731348 2316 10-11 0.0435909749461203 2316 12-13 -0.6631161030430377 2316 14-15 -0.9352821241487632 2316 16-17 -1.4630612348225327 2316 18-19 -0.1400563823833707 2316 20-21 -4.238730435313464 2316 22-23 -4.267570701452264 2316 24-25 -2.780300982028102 2316 26-27 -2.1922744182612064 2316 28-29 -1.4248751182129098 2316 30-31 -0.37942991479908983 2316 32-33 -1.8287253508589956 2316 34-35 -2.5700475255003496 2316 36-37 -4.8411730458471425 2316 38-39 -2.101226306425069 2316 40-41 -1.4940293078887095 2316 42-43 -3.035682287834245 2316 44-45 -0.6673296979473236 2316 46-47 -0.4977604014966772 2316 48-49 -0.23573365359062848 2316 50-51 0.14265927473893214 2316 52-53 -2.82632568283546 2316 54-55 -0.8132529984182568 2316 56-57 0.135061230438275 2316 58-59 0.28552512737882196 2316 60-61 -0.9305030477016807 2316 62-63 -0.6058095746753231 2316 64-65 0.6042159257942892 2316 66-67 1.286429367094481 2316 68-69 3.900858577193844 2316 70-71 4.147913558714311 2316 72-73 2.416257319705526 2316 74-75 1.2663422323322102 2316 76-77 0.06348535373948394 2316 78-79 0.4554309152868683 2316 80-81 0.06272946250254208 2316 82-83 0.3612794392942469 2316 84-85 0.8574084738322725 2316 86-87 0.13859203979498247 2316 88-89 0.6797023539074623 2316 90-91 0.7628613505441706 2316 92-93 0.9468011901222226 2316 94-95 1.4570958399560148 2316 96-97 1.5686241404421217 2316 98-99 0.24133492845024662 2316 100-101 0.5013173452899657 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11.0 3 28.0 4 65.0 5 158.0 6 401.0 7 1047.0 8 2198.0 9 3736.0 10 5840.0 11 8527.0 12 11605.0 13 14587.0 14 17713.0 15 22456.0 16 26500.0 17 30536.0 18 28894.0 19 25331.0 20 24616.0 21 24978.0 22 26843.0 23 30554.0 24 36722.0 25 46489.0 26 59083.0 27 73330.0 28 89944.0 29 107127.0 30 125629.0 31 146604.0 32 169679.0 33 195944.0 34 225943.0 35 257327.0 36 273362.0 37 230513.0 38 95268.0 39 5461.0 40 1.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.24409437867075 20.43712131718111 15.707837985218204 22.610946318929937 2 15.998262330073022 21.41355030754876 38.95812616350869 23.630061198869534 3 19.530684444080897 24.9747857917016 30.56285147542995 24.931678288787552 4 13.46487801885442 17.875585366352425 36.64951636980839 32.010020244984766 5 13.463463074533946 37.85141962506242 34.18813626077535 14.496981039628276 6 31.438784482934913 36.71679515756324 17.188114762479294 14.656305597022556 7 27.810269728635404 33.39629046440768 20.557248318030304 18.236191488926607 8 26.16731764176602 35.31698738267111 19.37236457332161 19.14333040224126 9 25.96695364102984 17.1822253123658 20.413652072554754 36.43716897404961 10-11 24.93329379767285 27.16983292775199 26.930185476779616 20.966687797795547 12-13 25.984233451258664 24.52326128300035 27.55403774973927 21.938467516001715 14-15 22.775423815463895 25.839819226600685 26.427230526983088 24.95752643095233 16-17 21.783679336322102 29.08384172620383 27.069499638349697 22.062979299124372 18-19 21.515605873025418 27.454213869969397 29.73166969937534 21.29851055762985 20-21 22.589706261570576 26.339200903703585 29.78925375539432 21.281839079331522 22-23 22.184536103556166 26.484182327559765 29.605815831987076 21.725465736896997 24-25 22.040858060162368 26.69209218625386 29.72963743072739 21.537412322856383 26-27 21.92532637502863 26.793140398521086 29.756936491836534 21.52459673461375 28-29 21.555246304532083 27.011604276499867 29.870580901049493 21.56256851791856 30-31 21.813854508316574 26.645267342266898 29.780460600825297 21.76041754859123 32-33 21.726309227825183 26.75406475341726 29.661876078424314 21.85774994033324 34-35 22.241237595383353 26.7390391587388 29.455676535413556 21.56404671046429 36-37 22.097380423777977 26.54677487168633 29.59647873802477 21.75936596651092 38-39 21.8927111976682 26.865740690512947 29.6832585113541 21.558289600464757 40-41 22.10769384474404 26.68249367997714 29.610218168745224 21.599594306533596 42-43 21.917689323766925 26.761532399209848 29.3010040633348 22.01977421368842 44-45 22.041832679516478 26.880091046512938 29.141945975930394 21.936130298040187 46-47 22.23033946827922 27.044737465882662 28.73493504861273 21.989988017225393 48-49 22.308229244707547 27.150018627463023 28.595018484174844 21.946733643654586 50-51 22.012073315990214 27.35905270464426 28.569024868181586 22.059849111183937 52-53 22.43292160318535 27.40051310051711 28.140264392477693 22.02630090381985 54-55 22.019562130606147 27.229643550470023 28.40177937261134 22.349014946312494 56-57 22.242840762520036 27.562519626084104 28.087184307724723 22.107455303671138 58-59 22.426892349894086 27.324924411949205 28.12386807111239 22.124315167044326 60-61 22.28389679982772 27.28908838538928 28.521734613344986 21.905280201438018 62-63 22.22530333825971 27.273521100089305 28.462354726571743 22.038820835079242 64-65 21.98415912046921 27.29077196168413 28.393375706561308 22.331693211285348 66-67 21.695326967298445 27.32153980086169 28.70279152211241 22.280341709727455 68-69 21.690161172931525 26.921157838959026 29.263763203417682 22.124917784691768 70-71 21.350889089844237 26.87551256340102 29.565337981262722 22.208260365492013 72-73 22.074611258277738 27.062367045029706 28.47152310383884 22.39149859285371 74-75 21.711659883191654 27.432635106116514 28.33756494102954 22.51814006966229 76-77 21.92168914460834 27.430785787826938 28.018732822485276 22.628792245079442 78-79 21.891277912369688 27.498007026229537 27.99167961571294 22.619035445687835 80-81 21.860313217148537 27.583616119832495 27.934698097719725 22.621372565299243 82-83 21.880472791967446 27.519641725118092 27.976605795382508 22.62327968753195 84-85 21.787918447475512 27.50434551440666 27.852927342999124 22.85480869511871 86-87 21.816081094621982 27.45540470625285 27.934291597905155 22.794222601220017 88-89 21.829752600172593 27.380482039075183 27.73164337451892 23.0581219862333 90-91 21.61782010324057 27.50045092596266 27.944035282246933 22.937693688549835 92-93 21.779434491144485 27.379594654645405 27.95114849622792 22.889822357982194 94-95 21.287291158750605 27.30997406233443 28.23278516828739 23.169949610627576 96-97 21.070999514413753 27.188771294617208 28.374921776339672 23.365307414629367 98-99 21.41647530046607 27.20889908054233 27.712068792024546 23.662556826967048 100-101 21.277617861945203 27.03646933109735 28.044971854245382 23.640940952712064 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1373.0 1 973.5 2 578.0 3 608.5 4 598.5 5 598.0 6 935.0 7 1808.0 8 2762.0 9 3417.5 10 3421.0 11 3013.5 12 2909.5 13 3018.5 14 3270.5 15 3443.0 16 3603.5 17 3879.5 18 4155.5 19 4543.0 20 5075.5 21 5766.0 22 6758.0 23 7989.0 24 9505.5 25 11525.0 26 14272.0 27 17577.5 28 20616.5 29 24045.0 30 28400.0 31 33360.5 32 38732.5 33 43923.0 34 49011.0 35 53964.5 36 59444.0 37 64646.0 38 67827.5 39 70350.0 40 73249.0 41 76678.0 42 80575.5 43 84510.0 44 87915.5 45 89934.5 46 91939.5 47 96174.0 48 102605.0 49 102464.5 50 96509.0 51 91732.0 52 86691.0 53 83072.5 54 79322.0 55 73014.0 56 65774.5 57 58539.0 58 51430.5 59 44100.5 60 36306.0 61 28936.0 62 22556.0 63 16881.5 64 12351.5 65 9092.5 66 6503.5 67 4729.0 68 3291.0 69 2145.0 70 1490.0 71 1041.5 72 745.5 73 571.0 74 411.5 75 294.0 76 197.0 77 108.5 78 62.5 79 35.5 80 22.5 81 14.5 82 8.5 83 3.0 84 2.5 85 2.5 86 2.0 87 1.5 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02278072023067013 2 0.01623688677123167 3 0.0 4 0.0 5 1.2269687736447108E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.47641152532668E-4 18-19 0.0016768573239811047 20-21 2.044947956074518E-5 22-23 0.0 24-25 0.0 26-27 4.0898959121490354E-4 28-29 0.017750148258726815 30-31 0.0046215823807284106 32-33 0.009059119445410114 34-35 0.009999795505204393 36-37 0.09828019876894133 38-39 0.1012044743461279 40-41 0.1150078730496309 42-43 0.10122492382568865 44-45 0.09580581174209117 46-47 0.09721682583178258 48-49 0.09897548107400667 50-51 0.0977689617799227 52-53 0.07623565980245803 54-55 0.06562237991043128 56-57 0.03752479499396741 58-59 0.015725649782213042 60-61 0.009345412159260547 62-63 0.0019835995173922826 64-65 0.004498885503363939 66-67 0.4257786139342754 68-69 0.8185926668166295 70-71 0.9070980143555346 72-73 0.28463630600601214 74-75 0.1513465982290751 76-77 0.014600928406372057 78-79 0.008629680374634465 80-81 0.0015132614874951433 82-83 0.0 84-85 0.0 86-87 2.0449479560745177E-4 88-89 8.179791824298071E-4 90-91 0.003312815688840719 92-93 0.0019835995173922826 94-95 0.22688697572646777 96-97 0.2343714852457005 98-99 0.014232837774278646 100-101 0.007668554835279442 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2445050.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.79332007200757 #Duplication Level Percentage of deduplicated Percentage of total 1 75.94267179382808 43.88979138104128 2 13.996173070453496 16.177706200878635 3 4.5749527322464525 7.932051227070742 4 2.0171818068447576 4.663185352256384 5 0.9886776446234954 2.85694817818821 6 0.5985401122245582 2.075497216903753 7 0.392836370044555 1.58923226489374 8 0.24795322698121036 1.1464032167849785 9 0.18302449344747115 0.9519833817744078 >10 0.9533311668677358 9.895269617237053 >50 0.06688275741238235 2.65538369314963 >100 0.03491773214240233 3.7055453420754225 >500 0.0020713923405114064 0.787915575790763 >1k 5.714185766920082E-4 0.6024435434105694 >5k 1.4285464417300206E-4 0.6521036491199647 >10k+ 7.142732208650103E-5 0.4185401594244048 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10139 0.41467454653279073 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9890 0.4044907057115396 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5907 0.24159015153064353 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2996 0.12253328152798512 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2970 0.12146990859082636 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.044947956074518E-5 0.0 12-13 0.0 0.0 0.0 4.089895912149036E-5 0.0 14-15 0.0 0.0 0.0 4.089895912149036E-5 4.089895912149036E-5 16-17 0.0 0.0 0.0 6.134843868223554E-5 8.179791824298072E-5 18-19 0.0 0.0 0.0 1.022473978037259E-4 8.179791824298072E-5 20-21 0.0 0.0 0.0 1.6359583648596144E-4 8.179791824298072E-5 22-23 0.0 0.0 0.0 3.680906320934132E-4 8.179791824298072E-5 24-25 0.0 0.0 0.0 7.770802233083168E-4 8.179791824298072E-5 26-27 0.0 0.0 0.0 0.001472362528373653 8.179791824298072E-5 28-29 0.0 0.0 0.0 0.0025357354655324025 8.179791824298072E-5 30-31 0.0 0.0 0.0 0.005746303756569395 8.179791824298072E-5 32-33 0.0 0.0 0.0 0.01306721743931617 8.179791824298072E-5 34-35 0.0 0.0 0.0 0.02637982863336128 8.179791824298072E-5 36-37 0.0 0.0 0.0 0.046174924848162616 8.179791824298072E-5 38-39 0.0 0.0 0.0 0.07353632850043966 8.179791824298072E-5 40-41 0.0 0.0 0.0 0.10504897650354798 8.179791824298072E-5 42-43 0.0 0.0 0.0 0.1378294922394225 8.179791824298072E-5 44-45 0.0 0.0 0.0 0.18144823214249198 8.179791824298072E-5 46-47 0.0 0.0 0.0 0.2339215966953641 1.2269687736447108E-4 48-49 4.089895912149036E-5 0.0 0.0 0.29058710455818904 1.2269687736447108E-4 50-51 4.089895912149036E-5 0.0 0.0 0.3490317171427987 1.2269687736447108E-4 52-53 4.089895912149036E-5 0.0 0.0 0.40843745526676345 1.6359583648596144E-4 54-55 4.089895912149036E-5 0.0 0.0 0.4765955706427272 2.044947956074518E-4 56-57 4.089895912149036E-5 0.0 0.0 0.5537719065049794 2.2494427516819696E-4 58-59 4.089895912149036E-5 0.0 0.0 0.6345678002494837 2.4539375472894216E-4 60-61 4.089895912149036E-5 0.0 0.0 0.714545714811558 2.4539375472894216E-4 62-63 4.089895912149036E-5 0.0 0.0 0.792478681417558 2.4539375472894216E-4 64-65 4.089895912149036E-5 0.0 0.0 0.875053679883847 2.4539375472894216E-4 66-67 4.089895912149036E-5 0.0 0.0 0.9622911596899859 2.6584323428968735E-4 68-69 4.089895912149036E-5 0.0 0.0 1.0695077810269729 2.862927138504325E-4 70-71 4.089895912149036E-5 0.0 0.0 1.2154352671724507 2.862927138504325E-4 72-73 4.089895912149036E-5 0.0 0.0 1.3728553608310667 2.862927138504325E-4 74-75 4.089895912149036E-5 0.0 0.0 1.546819083454326 2.862927138504325E-4 76-77 4.089895912149036E-5 0.0 0.0 1.7420707143003211 2.862927138504325E-4 78-79 4.089895912149036E-5 0.0 0.0 1.9664219545612565 2.862927138504325E-4 80-81 4.089895912149036E-5 0.0 0.0 2.2167235843847775 3.271916729719229E-4 82-83 4.089895912149036E-5 0.0 0.0 2.4760025357354656 4.4988855033639393E-4 84-85 4.089895912149036E-5 0.0 0.0 2.7310688942966403 4.4988855033639393E-4 86-87 4.089895912149036E-5 0.0 0.0 3.0058894501134947 4.4988855033639393E-4 88-89 4.089895912149036E-5 0.0 0.0 3.30377701887487 4.4988855033639393E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10790 0.0 32.5016 1 GTATCAA 15185 0.0 28.056879 1 ATCAACG 18360 0.0 22.96517 3 TATCAAC 18575 0.0 22.880697 2 TCAACGC 18520 0.0 22.817928 4 CAACGCA 18830 0.0 22.366795 5 AACGCAG 19130 0.0 22.0408 6 GTACATG 12030 0.0 21.903002 1 TACATGG 12135 0.0 21.201454 2 GAGTACT 10075 0.0 20.97254 12-13 ACATGGG 12415 0.0 19.995714 3 GTACTTT 10700 0.0 19.791784 14-15 ACGCAGA 21625 0.0 19.388288 7 CGCAGAG 21670 0.0 19.28244 8 AGTACTT 10380 0.0 19.146784 12-13 AGAGTAC 17775 0.0 18.483711 10-11 GTACCGT 180 1.2591493E-4 18.423742 6 GCAGAGT 22310 0.0 18.092241 9 ACTTTTT 12275 0.0 17.773352 16-17 GTATAGC 1030 0.0 17.484001 1 >>END_MODULE