FastQCFastQC Report
Fri 27 May 2016
SRR939442_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939442_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2406961
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71200.29580869818829636No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57320.2381426205077689No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36380.15114495000126715No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA132400.031.5628411
GGTATCA90750.030.8653811
ATCAACG166300.025.4865953
TATCAAC161850.025.4698432
TCAACGC167150.025.241754
CAACGCA167300.024.6821885
AACGCAG170650.024.1455026
GTACATG100200.023.1364251
GAGTACT84400.022.46850412-13
TACATGG102250.022.2860952
ACGCAGA187950.021.2524247
CGCAGAG190850.020.9321698
ACATGGG107750.020.6277943
GTACTTT94350.020.6199714-15
AGTACTT88700.020.376712-13
CAACGCG3850.020.1655145
GCAGAGT184000.020.0246859
AACGCGG3705.456968E-1219.6522886
AGAGTAC145750.019.47610710-11
TACACCG2352.063507E-618.583385
Produced by FastQC (version 0.11.5)