##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939442_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2406961 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.862551574371167 31.0 26.0 31.0 10.0 33.0 2 26.929463335716697 31.0 26.0 31.0 16.0 34.0 3 27.20420978985534 31.0 26.0 31.0 16.0 34.0 4 31.012150591555077 35.0 30.0 37.0 19.0 37.0 5 31.429159840977896 35.0 30.0 37.0 19.0 37.0 6 31.239918303620207 35.0 30.0 37.0 17.0 37.0 7 31.204837552415682 35.0 30.0 37.0 17.0 37.0 8 31.139649541475745 35.0 30.0 37.0 17.0 37.0 9 32.18939318086168 35.0 30.0 39.0 17.0 39.0 10-11 31.299998836707367 35.0 29.0 38.0 14.0 39.0 12-13 31.029914070065946 35.0 27.5 38.0 13.0 39.0 14-15 31.833299957913734 36.0 29.0 39.0 16.5 40.0 16-17 31.740287233569635 36.0 28.0 39.0 16.0 40.0 18-19 31.67559528384548 36.0 28.0 38.5 13.5 40.0 20-21 31.55583638455297 36.0 27.0 39.0 11.0 40.0 22-23 31.741675290958185 36.0 28.0 39.0 13.5 40.0 24-25 31.81414405966694 36.0 29.0 39.0 13.0 41.0 26-27 31.91690559173996 36.0 30.0 39.0 14.5 41.0 28-29 31.519961062933717 36.0 29.0 39.0 10.0 40.0 30-31 31.237613945552088 36.0 27.5 39.0 10.0 40.0 32-33 31.107501534092158 35.0 28.0 39.0 9.5 40.0 34-35 30.65594124707463 35.0 27.0 39.0 9.0 40.0 36-37 30.302700376117436 35.0 26.0 38.0 9.0 40.0 38-39 29.927897045278257 35.0 25.0 38.0 8.0 40.0 40-41 29.788351992408685 34.5 25.0 38.0 8.0 40.0 42-43 29.523480646342005 34.0 25.0 38.0 7.5 40.0 44-45 29.172695984687746 34.0 24.5 38.0 2.0 40.0 46-47 28.60407480636371 34.0 24.0 38.0 2.0 40.0 48-49 28.26979207390564 33.0 23.0 38.0 2.0 40.0 50-51 27.61816228015327 33.0 22.5 37.5 2.0 39.5 52-53 27.75602429785942 33.0 23.0 38.0 2.0 39.5 54-55 27.880441145494256 33.5 23.0 38.0 2.0 40.0 56-57 27.424550086187523 33.0 21.5 38.0 2.0 40.0 58-59 27.031901638622312 33.0 20.0 38.0 2.0 40.0 60-61 26.420871588696286 32.5 17.5 37.5 2.0 39.5 62-63 25.86018115789994 31.5 14.5 37.0 2.0 39.0 64-65 25.333882435153704 31.0 13.0 36.0 2.0 39.0 66-67 24.95161928257251 31.0 10.5 36.0 2.0 39.0 68-69 24.243461983804472 30.5 8.0 35.5 2.0 38.5 70-71 23.619787981608344 30.0 7.0 35.0 2.0 38.0 72-73 23.122445066621353 29.0 2.0 34.5 2.0 37.5 74-75 22.3770796452456 29.0 2.0 34.0 2.0 36.5 76-77 21.594344902140087 28.0 2.0 34.0 2.0 36.0 78-79 21.064114873485693 27.5 2.0 34.0 2.0 36.0 80-81 20.491114313858844 26.5 2.0 33.0 2.0 35.0 82-83 19.74072741519285 26.0 2.0 33.0 2.0 35.0 84-85 19.027238912470956 25.5 2.0 32.0 2.0 35.0 86-87 18.236332869539638 24.0 2.0 32.0 2.0 34.0 88-89 17.4294853551844 20.5 2.0 31.5 2.0 34.0 90-91 16.605071083411822 18.5 2.0 31.0 2.0 34.0 92-93 15.756518281766926 9.5 2.0 31.0 2.0 34.0 94-95 14.687766856214122 2.0 2.0 30.5 2.0 34.0 96-97 13.586439082311678 2.0 2.0 29.5 2.0 34.0 98-99 12.550230560445309 2.0 2.0 29.0 2.0 34.0 100-101 11.233949366026287 2.0 2.0 27.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 3.9081167936613532 1101 2 3.9516730714679973 1101 3 3.5110787922658275 1101 4 2.977353940191719 1101 5 2.395793181480606 1101 6 2.741904874950375 1101 7 2.587603123382557 1101 8 2.6179619597758226 1101 9 2.7513005560218495 1101 10-11 3.5132406987444114 1101 12-13 3.4236873157591923 1101 14-15 2.9272077142307076 1101 16-17 2.9150223349568343 1101 18-19 2.890112857640357 1101 20-21 2.8623618643638515 1101 22-23 2.8317364403362255 1101 24-25 3.101853017376339 1101 26-27 2.8795044333868454 1101 28-29 2.9992497765766437 1101 30-31 3.1999085029177188 1101 32-33 2.9164898183443846 1101 34-35 3.15256673588296 1101 36-37 3.1166199179249965 1101 38-39 3.3012550539768135 1101 40-41 3.119956746984137 1101 42-43 2.9879201473976984 1101 44-45 2.97896509896729 1101 46-47 3.044995635742623 1101 48-49 2.857208763556798 1101 50-51 3.070165420523171 1101 52-53 3.03826132745856 1101 54-55 3.0029721636211306 1101 56-57 3.1344855196309567 1101 58-59 2.9378750272728347 1101 60-61 3.093434966375998 1101 62-63 3.1901643343514117 1101 64-65 2.9082957931945153 1101 66-67 2.6407496320648676 1101 68-69 3.0523379875041172 1101 70-71 2.609483520297605 1101 72-73 2.5608261823696417 1101 74-75 2.448465170545859 1101 76-77 2.400891035885479 1101 78-79 1.991835474210614 1101 80-81 2.157305487039711 1101 82-83 1.768881148909994 1101 84-85 1.8177407800181946 1101 86-87 1.7601419346739675 1101 88-89 1.6512090060921203 1101 90-91 1.4948837180672392 1101 92-93 1.4658533544805383 1101 94-95 1.3918289237474912 1101 96-97 1.1188225231028497 1101 98-99 0.8314655810300202 1101 100-101 0.8700983806391722 1102 1 3.5888668322865485 1102 2 2.440005339998695 1102 3 2.7890152465148077 1102 4 2.8754777997045835 1102 5 1.9884134728607137 1102 6 2.5758512005461434 1102 7 2.494176504986946 1102 8 2.356717435158007 1102 9 2.45321093722119 1102 10-11 1.756971725422943 1102 12-13 2.9377283043971225 1102 14-15 3.0885146655128572 1102 16-17 2.7869013149176602 1102 18-19 2.838450512819687 1102 20-21 2.8883720697663655 1102 22-23 2.772675596260914 1102 24-25 2.9536638082115196 1102 26-27 2.5467621699087424 1102 28-29 2.72197955472188 1102 30-31 2.8944934595531144 1102 32-33 2.669380653107897 1102 34-35 2.800259037151111 1102 36-37 2.847271201325345 1102 38-39 2.951613119961177 1102 40-41 2.7747464797813137 1102 42-43 2.7045020474231656 1102 44-45 2.4558513655187078 1102 46-47 2.476139442558196 1102 48-49 2.495949832785673 1102 50-51 2.683483387292977 1102 52-53 2.8390000081889717 1102 54-55 2.6943119776356035 1102 56-57 2.6844195436245073 1102 58-59 2.765800624710625 1102 60-61 2.8621445926093934 1102 62-63 2.753767709984551 1102 64-65 2.6845895283968915 1102 66-67 2.4574699564329805 1102 68-69 2.5015742525940396 1102 70-71 2.3705608082661698 1102 72-73 2.1199544639452164 1102 74-75 1.9902175433854588 1102 76-77 2.103077848200442 1102 78-79 2.1271814933102497 1102 80-81 2.1163423208595127 1102 82-83 1.9145121175463373 1102 84-85 1.6680988460025503 1102 86-87 1.5209373631213197 1102 88-89 1.3566415873824944 1102 90-91 1.212362348939834 1102 92-93 0.8980177015477384 1102 94-95 0.774147562804453 1102 96-97 0.688000871280785 1102 98-99 0.4677538460698347 1102 100-101 0.11116990614641331 1103 1 3.804541092967561 1103 2 3.7686958359649516 1103 3 3.3877358280351046 1103 4 3.130461898257444 1103 5 2.6714674976562165 1103 6 2.7885233257867945 1103 7 2.433375036741289 1103 8 2.424195665462964 1103 9 2.899079008575878 1103 10-11 3.489647453868116 1103 12-13 2.6676318123454372 1103 14-15 3.301925688429723 1103 16-17 2.570809154736363 1103 18-19 2.597831684511174 1103 20-21 2.9614828149395613 1103 22-23 3.331737053769178 1103 24-25 3.1778573604816565 1103 26-27 3.0634919929665223 1103 28-29 2.657920958109859 1103 30-31 3.1523938265342757 1103 32-33 2.9769657196307513 1103 34-35 2.771333490270539 1103 36-37 3.130907998309251 1103 38-39 3.4570167230052 1103 40-41 3.184443272929286 1103 42-43 3.177133542319698 1103 44-45 3.1109476529340476 1103 46-47 2.9057034146859237 1103 48-49 2.9074440150949776 1103 50-51 3.090933193209459 1103 52-53 3.131462650020012 1103 54-55 3.0749646552017644 1103 56-57 3.353128360561655 1103 58-59 3.412938364225422 1103 60-61 3.4609408093248923 1103 62-63 3.418975439941626 1103 64-65 3.385535590270891 1103 66-67 2.8325302439028945 1103 68-69 2.961458508676756 1103 70-71 2.9571257907356596 1103 72-73 2.5242299991494868 1103 74-75 2.7261865124917897 1103 76-77 2.6880837826542177 1103 78-79 2.4641027837550133 1103 80-81 2.43827741021337 1103 82-83 2.608767173066987 1103 84-85 2.4657572445586986 1103 86-87 2.4057561957633027 1103 88-89 2.1917961841164946 1103 90-91 1.8954585047459318 1103 92-93 1.894356516727422 1103 94-95 2.0412924766284792 1103 96-97 1.9653465052642218 1103 98-99 1.7691432465495662 1103 100-101 1.5546318955486598 1104 1 3.696342454842064 1104 2 3.5132795563611445 1104 3 2.717211601567392 1104 4 2.5995024704463674 1104 5 1.4760682981595536 1104 6 2.2454584653867933 1104 7 2.3018666131066254 1104 8 2.289066520489815 1104 9 2.2684216564885666 1104 10-11 3.2770047206882467 1104 12-13 3.306283729416773 1104 14-15 3.102398085146948 1104 16-17 3.0507056232561816 1104 18-19 2.7661506861837175 1104 20-21 2.6489089862831214 1104 22-23 2.2993856665705685 1104 24-25 2.3834177434878576 1104 26-27 2.5514441697333616 1104 28-29 2.387127447910469 1104 30-31 2.0858251491143953 1104 32-33 2.287807266964709 1104 34-35 2.822958940910386 1104 36-37 2.896858188620161 1104 38-39 3.0299621693456906 1104 40-41 2.7533883362742415 1104 42-43 2.591743941715226 1104 44-45 2.4387906946440303 1104 46-47 2.6134268156329057 1104 48-49 2.483287532082141 1104 50-51 2.657469932109187 1104 52-53 2.6765033646320653 1104 54-55 2.553614921338319 1104 56-57 2.6521511218580898 1104 58-59 2.7202462710294846 1104 60-61 2.8732228972693044 1104 62-63 3.0020983391004457 1104 64-65 2.8193373063362515 1104 66-67 2.7705498449527752 1104 68-69 2.66593824952 1104 70-71 2.6445813668545526 1104 72-73 2.6233456288610846 1104 74-75 2.7801977505835254 1104 76-77 2.509582594061193 1104 78-79 2.390357540860901 1104 80-81 2.5082675766163938 1104 82-83 2.2923438525864057 1104 84-85 2.1426650410515187 1104 86-87 2.2222847333311364 1104 88-89 2.3469307779745385 1104 90-91 2.2438602185175824 1104 92-93 2.026727013696126 1104 94-95 2.0679035982746345 1104 96-97 2.0631605235941315 1104 98-99 1.7224915369785982 1104 100-101 1.5658447165107834 1105 1 3.5132556126279972 1105 2 3.0224306557009974 1105 3 2.7131820534394038 1105 4 2.5903688009998334 1105 5 0.14794771896825054 1105 6 1.9123746902934933 1105 7 1.9095363336527384 1105 8 2.01170935711443 1105 9 2.489061676766692 1105 10-11 2.4108844744214224 1105 12-13 3.0226406574210394 1105 14-15 3.0156677466746338 1105 16-17 2.673716622464678 1105 18-19 2.8948010665742423 1105 20-21 2.6477478040877678 1105 22-23 2.56795492094135 1105 24-25 2.203796545062442 1105 26-27 2.461794526802965 1105 28-29 2.204397918509482 1105 30-31 2.4367907787159204 1105 32-33 2.486675593984092 1105 34-35 2.9160803753824958 1105 36-37 2.888647290153745 1105 38-39 2.883975868431147 1105 40-41 2.8901990819520904 1105 42-43 2.800485019704137 1105 44-45 2.648973116718217 1105 46-47 2.837413578101831 1105 48-49 2.6917155475685313 1105 50-51 2.64084904255769 1105 52-53 2.629757539226631 1105 54-55 2.5659021854368724 1105 56-57 2.889676857973832 1105 58-59 2.875865492986069 1105 60-61 2.9540678093213053 1105 62-63 2.9464178693094247 1105 64-65 2.780955262531279 1105 66-67 2.437446815907542 1105 68-69 2.7465440747795107 1105 70-71 2.7195032228328273 1105 72-73 2.682237928063987 1105 74-75 2.660075992645016 1105 76-77 2.895516035108564 1105 78-79 2.600410116985625 1105 80-81 2.766845989112518 1105 82-83 2.7054060443373906 1105 84-85 2.5834098893020006 1105 86-87 2.462521155646911 1105 88-89 2.4645918132993963 1105 90-91 2.362513136376304 1105 92-93 2.47024260696511 1105 94-95 2.36095685348962 1105 96-97 2.3330251298041986 1105 98-99 2.226601478800589 1105 100-101 1.965347397066239 1106 1 3.0751205384288696 1106 2 2.5270857697806477 1106 3 2.0743851131280415 1106 4 1.3375431465489314 1106 5 1.6653185337737924 1106 6 2.1370105541525923 1106 7 2.103826644052319 1106 8 0.484473008835991 1106 9 1.8676683486400272 1106 10-11 0.9118488946517189 1106 12-13 1.4870305450276255 1106 14-15 1.6804608715802836 1106 16-17 1.6027760277234115 1106 18-19 1.895059354458322 1106 20-21 1.9013809831984112 1106 22-23 2.4430991169030314 1106 24-25 2.7107534760350767 1106 26-27 2.3854792530294056 1106 28-29 1.8605257849220074 1106 30-31 2.0165805972911848 1106 32-33 2.3708278867390966 1106 34-35 2.8344572446796867 1106 36-37 1.7223946477827745 1106 38-39 2.7882685476060907 1106 40-41 2.2502681432774665 1106 42-43 2.3192256194366045 1106 44-45 2.5194272874606902 1106 46-47 2.9588855926641386 1106 48-49 2.6949881941588387 1106 50-51 2.4622909735364615 1106 52-53 2.486023536472196 1106 54-55 2.688918745181354 1106 56-57 2.759531129876784 1106 58-59 2.7324372354460493 1106 60-61 2.823229995156847 1106 62-63 3.1579617149050954 1106 64-65 3.0465506208770776 1106 66-67 2.600349284411493 1106 68-69 2.968758796561591 1106 70-71 2.9084112855741466 1106 72-73 2.8706070500883705 1106 74-75 2.9902358215695237 1106 76-77 3.212560185290286 1106 78-79 2.7814713556246744 1106 80-81 2.706122562115052 1106 82-83 3.0297779881637297 1106 84-85 3.0819824873576422 1106 86-87 3.0588806056491826 1106 88-89 3.0887695775491046 1106 90-91 2.4687890546586004 1106 92-93 2.4730274522030715 1106 94-95 2.4887805135053025 1106 96-97 2.503586775035288 1106 98-99 2.3253408866449305 1106 100-101 1.9117097722401635 1107 1 3.2138584798630028 1107 2 3.4270830830440104 1107 3 -0.21713403383454377 1107 4 0.9790846169162073 1107 5 1.5133015066170543 1107 6 2.103025391105117 1107 7 2.247419944358853 1107 8 2.1523447918909646 1107 9 2.5290416058031546 1107 10-11 1.6832482748230433 1107 12-13 2.2711333445631645 1107 14-15 2.7479071372145576 1107 16-17 1.2499181694518562 1107 18-19 0.4823687521492239 1107 20-21 -1.3341562620919767 1107 22-23 0.6216459065683999 1107 24-25 1.423737475857724 1107 26-27 1.4586353478767826 1107 28-29 2.365062405803055 1107 30-31 2.922107082421565 1107 32-33 1.6233679906878997 1107 34-35 1.008159539872178 1107 36-37 1.423925294859071 1107 38-39 2.462193342764447 1107 40-41 2.619205533064374 1107 42-43 1.8650780101256963 1107 44-45 1.4399048747183336 1107 46-47 2.162457939361225 1107 48-49 1.8483347889110107 1107 50-51 2.431479316270682 1107 52-53 1.9765468437657177 1107 54-55 2.5396960709446503 1107 56-57 2.948816203938957 1107 58-59 2.815705443555675 1107 60-61 2.6801712526699824 1107 62-63 2.5673191488043976 1107 64-65 2.0112009479762563 1107 66-67 2.578009069264084 1107 68-69 2.9667828706027706 1107 70-71 1.3844928695801073 1107 72-73 2.3944448639832636 1107 74-75 3.1282659620564885 1107 76-77 3.2235663358617614 1107 78-79 2.892704381285064 1107 80-81 2.990033105823926 1107 82-83 2.730525150536735 1107 84-85 2.921342577162182 1107 86-87 2.92939472812062 1107 88-89 2.9002133725601986 1107 90-91 3.006803588582464 1107 92-93 2.8419649153980853 1107 94-95 2.8236727658230567 1107 96-97 2.5948450149115416 1107 98-99 2.5367703230307157 1107 100-101 2.119419693360218 1108 1 2.4392274290978193 1108 2 2.28039192829209 1108 3 1.739521541578803 1108 4 1.7244887571975198 1108 5 1.9266045743208338 1108 6 -0.11283100670177149 1108 7 0.6559350837458879 1108 8 -0.3396934831313274 1108 9 1.451641095826087 1108 10-11 2.929552663475306 1108 12-13 1.7498634753783087 1108 14-15 1.9901744970052313 1108 16-17 0.18126435389300966 1108 18-19 0.5794867184536336 1108 20-21 1.2696608756092367 1108 22-23 -0.5300978043464859 1108 24-25 1.0777427992362725 1108 26-27 1.3987882835839294 1108 28-29 2.0560946266418334 1108 30-31 0.6926899239308639 1108 32-33 1.0804209233582114 1108 34-35 1.164854276882867 1108 36-37 0.07917156646197299 1108 38-39 1.5942433934614861 1108 40-41 0.23488990388541708 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54-55 -0.9584995143579818 2312 56-57 -0.46365438663467273 2312 58-59 -0.5222379484867368 2312 60-61 0.030133683430928926 2312 62-63 -0.4534844276000918 2312 64-65 -2.1759558283803813 2312 66-67 0.959987487217191 2312 68-69 0.18512289482012179 2312 70-71 -2.1157682444257766 2312 72-73 -2.766673292442732 2312 74-75 -0.6541723867832516 2312 76-77 -0.04010074144240505 2312 78-79 -0.10657092991801065 2312 80-81 -1.64488040893038 2312 82-83 0.05222043127131215 2312 84-85 1.4661365874407188 2312 86-87 1.7088323119352964 2312 88-89 2.0694681531874117 2312 90-91 2.438303349487775 2312 92-93 2.6788907422623485 2312 94-95 2.091729091621625 2312 96-97 1.5075565513570162 2312 98-99 1.1124044305995522 2312 100-101 1.9396832314394086 2313 1 -8.143483581853136 2313 2 -4.683913348809785 2313 3 -2.166910920752702 2313 4 0.18026058857377691 2313 5 0.5896810598048425 2313 6 -2.537466950507163 2313 7 -0.9538249272476733 2313 8 -2.5139907722432078 2313 9 0.3835577796701948 2313 10-11 -1.5601746508826153 2313 12-13 -2.225054389582823 2313 14-15 -3.9312869788881954 2313 16-17 -3.630789488460689 2313 18-19 -1.0327962652084146 2313 20-21 -2.99786164987 2313 22-23 -1.591522318992606 2313 24-25 -2.5028778418680595 2313 26-27 -1.7691447155666076 2313 28-29 -2.507615735212962 2313 30-31 -4.318848710341644 2313 32-33 -5.723535530772647 2313 34-35 -4.477860799075973 2313 36-37 -2.0426678323314214 2313 38-39 -1.4884066807565723 2313 40-41 -3.466239412114632 2313 42-43 -2.3625644514081365 2313 44-45 -2.6244210481746997 2313 46-47 -1.3604783190785241 2313 48-49 -2.313464366945823 2313 50-51 -3.1650893078804927 2313 52-53 -0.3964993576098266 2313 54-55 -1.6277486239770695 2313 56-57 -2.205596402217452 2313 58-59 -1.6456979137725263 2313 60-61 -2.017454292738446 2313 62-63 -4.080119020421005 2313 64-65 -5.643788992235223 2313 66-67 -4.15621848509263 2313 68-69 -2.6002141132425685 2313 70-71 -1.6969265762821522 2313 72-73 -1.8959042726488988 2313 74-75 -2.798678709304614 2313 76-77 -2.4920050724017173 2313 78-79 -2.8202215065244367 2313 80-81 -3.642924392806986 2313 82-83 -1.5449885203614713 2313 84-85 -1.3173906972762133 2313 86-87 -2.8550164406403002 2313 88-89 -3.9013287389698093 2313 90-91 -3.4093449755465564 2313 92-93 -1.8184915824016716 2313 94-95 -0.9197317469652102 2313 96-97 -1.218124192750924 2313 98-99 -1.3283195181469836 2313 100-101 -0.9554708883731369 2314 1 -7.764016310992936 2314 2 -4.200228663802381 2314 3 -24.82037130189613 2314 4 -13.368200270193 2314 5 -7.1193299016509535 2314 6 -5.413654043532272 2314 7 -4.721656696364548 2314 8 -3.642962508881695 2314 9 -3.468695408465642 2314 10-11 -14.375406059393868 2314 12-13 -13.016698916417294 2314 14-15 -5.208727683939859 2314 16-17 -3.6557311288580756 2314 18-19 -3.6788490067664235 2314 20-21 -4.883705192402356 2314 22-23 -4.769355670236816 2314 24-25 -5.359704522306199 2314 26-27 -4.331883278477562 2314 28-29 -3.8245143477171375 2314 30-31 -4.009226888526992 2314 32-33 -4.263517580095218 2314 34-35 -3.3103065490242294 2314 36-37 -4.0019703430381455 2314 38-39 -1.293868701740159 2314 40-41 -2.3846941811837397 2314 42-43 -4.651859917170501 2314 44-45 -5.529153847763947 2314 46-47 -4.613057733910324 2314 48-49 -3.2491404427924095 2314 50-51 -3.0408868393638357 2314 52-53 -1.5188890962702537 2314 54-55 -3.724912387091809 2314 56-57 -3.5947357946009504 2314 58-59 -1.5139106870128813 2314 60-61 -4.158806424530649 2314 62-63 -5.043221124351845 2314 64-65 -4.475986726310463 2314 66-67 -5.7937195298937 2314 68-69 -5.057136218845091 2314 70-71 -5.478976595387088 2314 72-73 -4.746457917711073 2314 74-75 -4.319761053559603 2314 76-77 -4.207201007429024 2314 78-79 -4.920949533860412 2314 80-81 -7.247667441397201 2314 82-83 -6.763462643126594 2314 84-85 -5.267859166174974 2314 86-87 -4.523157769388449 2314 88-89 -3.7636433614085547 2314 90-91 -5.590576666651618 2314 92-93 -5.451237040999688 2314 94-95 -5.87095272139644 2314 96-97 -5.3161208115756535 2314 98-99 -6.059161100433526 2314 100-101 -5.167930439646004 2315 1 -24.32502821575484 2315 2 -24.451716759040476 2315 3 -13.391725468562798 2315 4 -28.62415265114538 2315 5 -15.705416211174763 2315 6 -12.488009202262429 2315 7 -9.782370982078834 2315 8 -8.663126199357887 2315 9 -6.387073384656119 2315 10-11 -28.946834630822437 2315 12-13 -28.654843757687136 2315 14-15 -23.54893105695573 2315 16-17 -14.471914164572357 2315 18-19 -9.598119840099752 2315 20-21 -7.631510251926166 2315 22-23 -4.465933051189193 2315 24-25 -6.170021982623659 2315 26-27 -6.271640222922006 2315 28-29 -17.591441431050473 2315 30-31 -16.596751690114296 2315 32-33 -10.121578095968236 2315 34-35 -7.930309029182961 2315 36-37 -5.881905858911161 2315 38-39 -5.89763854301 2315 40-41 -9.17126412165993 2315 42-43 -8.597643647329232 2315 44-45 -5.7426583120496595 2315 46-47 -4.39052549184683 2315 48-49 -5.448433597554313 2315 50-51 -3.8590292004749465 2315 52-53 -7.419757151825809 2315 54-55 -7.677008121218794 2315 56-57 -8.226965457299364 2315 58-59 -8.32419094494939 2315 60-61 -5.63631138484811 2315 62-63 -4.303191362447084 2315 64-65 -5.447278889008878 2315 66-67 -7.681219529893703 2315 68-69 -7.426741873606993 2315 70-71 -8.372131357291849 2315 72-73 -7.87803033834599 2315 74-75 -8.328528414670714 2315 76-77 -7.798780868540135 2315 78-79 -7.576802708463587 2315 80-81 -8.641764663619423 2315 82-83 -7.721175936777389 2315 84-85 -7.03323615030196 2315 86-87 -7.406081876531307 2315 88-89 -6.731760920932363 2315 90-91 -6.103547896810349 2315 92-93 -5.816254402110799 2315 94-95 -5.972242403936123 2315 96-97 -5.893253748083589 2315 98-99 -5.662224096465272 2315 100-101 -5.450817344407909 2316 1 -1.3283876782408441 2316 2 1.2907244503660174 2316 3 1.6516331773871826 2316 4 2.1272471248904523 2316 5 -1.7472794297264613 2316 6 -2.9023814027103576 2316 7 -3.319462714068383 2316 8 -4.172662756842023 2316 9 -3.5498224281424946 2316 10-11 -3.3659275759512184 2316 12-13 -3.8740486848987814 2316 14-15 -2.3594999492662936 2316 16-17 -2.4090464317429436 2316 18-19 -4.128795348685049 2316 20-21 -2.0739330892721917 2316 22-23 -2.5780971049405963 2316 24-25 -2.8402895731425097 2316 26-27 -3.048869949499089 2316 28-29 -2.2860321715319962 2316 30-31 0.3303648878998935 2316 32-33 -2.0734135522952215 2316 34-35 -7.49053993390487 2316 36-37 -3.415659708295262 2316 38-39 -1.656461797955874 2316 40-41 -1.7475994496741158 2316 42-43 -1.6941920329208706 2316 44-45 -0.7788597281564158 2316 46-47 0.18343954874667645 2316 48-49 0.653581788317279 2316 50-51 0.8842445840528654 2316 52-53 0.5709862458972204 2316 54-55 -1.2225647071650414 2316 56-57 -0.3826033469036929 2316 58-59 -3.6334326746059773 2316 60-61 -3.9240534294543075 2316 62-63 -5.605679464350779 2316 64-65 -5.64247036670578 2316 66-67 -3.1172019393748513 2316 68-69 -2.89352489665664 2316 70-71 -3.5793588675568735 2316 72-73 -3.475463499040277 2316 74-75 -3.1336472206617536 2316 76-77 -2.937625528394559 2316 78-79 -3.1731411276498527 2316 80-81 -3.3482666233058715 2316 82-83 -4.152992262831885 2316 84-85 -3.4457618128626155 2316 86-87 -2.8393805070837512 2316 88-89 -4.041263942115641 2316 90-91 -4.322520111256038 2316 92-93 -4.690147704182467 2316 94-95 -5.643095567429965 2316 96-97 -5.545526051181761 2316 98-99 -5.422324938663854 2316 100-101 -5.150059132388499 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 37344.0 3 13629.0 4 12366.0 5 12425.0 6 14490.0 7 18024.0 8 21519.0 9 26171.0 10 30484.0 11 34944.0 12 38800.0 13 42318.0 14 45304.0 15 48884.0 16 51153.0 17 52157.0 18 51021.0 19 52635.0 20 55990.0 21 59857.0 22 65423.0 23 71019.0 24 76285.0 25 83297.0 26 89777.0 27 97211.0 28 103717.0 29 110924.0 30 117579.0 31 126042.0 32 132216.0 33 137781.0 34 140105.0 35 135773.0 36 117071.0 37 69882.0 38 13249.0 39 95.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.742203597875516 19.148028700739214 14.803217183302825 22.306550518082446 2 15.819403719977714 20.47631866343862 40.33604855019005 23.36822906639361 3 19.820009197161635 24.982965111205097 29.77782477057445 25.419200921058817 4 12.93370541169448 17.132623969878253 37.336906559956134 32.59676405847113 5 13.699913939617906 37.42519654892874 34.0081249656674 14.866764545785955 6 31.303455012643944 35.91790108491298 17.919257590003614 14.859386312439469 7 28.133982394506766 33.20441933809733 20.699637044725673 17.961961222670233 8 26.38300880833756 34.325442704217856 19.828948572284098 19.46259991516049 9 26.711067214222588 17.316088787232935 20.63639860685138 35.33644539169309 10-11 25.067845309100793 26.872345522547903 27.087864723378264 20.97194444497304 12-13 26.55094434969364 24.394942412566063 27.435348367127787 21.618764870612512 14-15 23.35347244057335 25.634937238056942 26.50643486859055 24.50515545277916 16-17 22.1095928918563 28.970771264529848 26.958933229540527 21.960702614073323 18-19 21.85198916345742 27.193762562943373 29.85087382048426 21.103374453114945 20-21 23.021138786971353 26.093445597110364 29.787351752185536 21.098063863732744 22-23 22.62023701579183 26.27804580435952 29.61651085346445 21.485206326384194 24-25 22.292146369431943 26.41688975359054 29.93222512459443 21.358738752383083 26-27 22.070106254883573 26.60909286838258 29.916295882696076 21.404504994037776 28-29 21.689786177336355 26.88683550770846 29.98415300671999 21.439225308235194 30-31 21.951110385994294 26.50326011225807 29.977529240289375 21.568100261458255 32-33 21.823202120882126 26.632679344743632 29.824488764959007 21.71962976941524 34-35 22.458972161876716 26.64718274714112 29.545160942043143 21.348684148939025 36-37 22.27800928786001 26.404816907349787 29.65440308047773 21.66277072431247 38-39 22.04163348034751 26.75387523724133 29.792087102040576 21.412404180370583 40-41 22.26218241732004 26.586868292333925 29.66990410801066 21.481045182335375 42-43 22.046686798044043 26.66455633149428 29.393003406971136 21.895753463490543 44-45 22.195291946215896 26.806796080358758 29.18623953256824 21.811672440857098 46-47 22.391295394212158 26.97738438311382 28.695116223244817 21.936203999429214 48-49 22.404020410791414 27.068889437663014 28.63569042454105 21.891399727004522 50-51 22.219628282954385 27.300406256664743 28.54218879230973 21.937776668071137 52-53 22.362444817788667 27.509954285103046 28.23683092771877 21.89076996938952 54-55 22.04403079171227 27.24360234981278 28.413680756678172 22.29868610179678 56-57 22.24943427841137 27.615223189831344 28.176107773057275 21.959234758700006 58-59 22.376691128750437 27.302992861065352 28.259427054731834 22.06088895545238 60-61 22.240719905332533 27.30189397326767 28.634018313746125 21.823367807653675 62-63 22.18619116766822 27.29848063661272 28.523326202456722 21.992001993262342 64-65 21.99169166619007 27.359748774286796 28.403188154125314 22.24537140539782 66-67 21.9878384308082 27.544623267943408 28.071908813186404 22.39562948806199 68-69 22.04566084702462 27.35475069743164 28.22096963533917 22.378618820204565 70-71 21.994633573216202 27.336892509860043 28.198262036451023 22.470211880472732 72-73 22.090645388426005 27.252629531671385 28.209567927878084 22.44715715202453 74-75 21.9176352402328 27.57812956692379 28.00871149325887 22.495523699584542 76-77 22.07920456278606 27.35792739550534 27.96914114428785 22.59372689742075 78-79 22.004559344513076 27.49930606909679 27.879813248316566 22.616321338073565 80-81 21.936186486736176 27.590592933339213 27.859901906032537 22.613318673892074 82-83 21.992429188528355 27.568839082597744 27.80658686397215 22.632144864901754 84-85 21.85913894342766 27.533202773135223 27.748015489149314 22.859642794287804 86-87 21.905490601858034 27.420660726180536 27.895404506831063 22.77844416513037 88-89 22.014772150008092 27.346970785624926 27.647356419203543 22.990900645163435 90-91 21.790066374650106 27.451061567922974 27.81931033191486 22.93956172551206 92-93 21.938917939989658 27.364798664014558 27.861716020717093 22.834567375278695 94-95 21.739705046477898 27.247808786167543 27.820033354444355 23.192452812910204 96-97 21.794579078194786 27.186945397623692 27.671472882382826 23.347002641798696 98-99 21.72951503885393 27.195210548118297 27.338677613350846 23.736596799676928 100-101 21.652048871922133 27.145996517715222 27.730118348542476 23.471836261820176 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2435.0 1 1592.5 2 705.0 3 672.5 4 627.0 5 624.0 6 900.5 7 1562.5 8 2392.0 9 2980.0 10 2949.5 11 2590.0 12 2548.5 13 2735.0 14 2941.0 15 3155.5 16 3343.0 17 3643.5 18 4050.5 19 4494.5 20 5028.5 21 5748.0 22 6747.0 23 8092.0 24 9656.5 25 11603.0 26 14301.5 27 17324.0 28 20432.0 29 23961.5 30 27932.5 31 32576.5 32 37871.5 33 43096.0 34 48343.0 35 53132.0 36 58157.5 37 63556.5 38 67172.0 39 69954.5 40 72975.5 41 76388.5 42 80071.0 43 83545.5 44 86591.5 45 88705.5 46 90590.5 47 93891.5 48 98571.0 49 99420.5 50 95331.0 51 90254.0 52 85412.0 53 81048.5 54 76421.5 55 70554.0 56 63672.5 57 56509.5 58 49590.5 59 42752.5 60 35723.5 61 28937.0 62 22735.0 63 17458.5 64 13147.5 65 9724.0 66 7253.0 67 5390.0 68 3839.5 69 2727.0 70 2008.0 71 1441.0 72 1067.5 73 808.5 74 595.0 75 434.5 76 295.0 77 209.0 78 143.0 79 101.5 80 67.0 81 34.5 82 19.5 83 22.5 84 20.0 85 9.5 86 8.5 87 8.0 88 7.0 89 5.0 90 4.5 91 5.0 92 3.5 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.246516665621089 2 2.459740726999731 3 0.98119579004396 4 0.8908328801339117 5 0.16606002340710962 6 0.09356196465169149 7 0.09376969547907091 8 0.10058326661711593 9 0.09705184255166577 10-11 1.7535182331579116 12-13 1.3423981526912983 14-15 0.5034564332367663 16-17 0.09015517908266897 18-19 0.09636633082131368 20-21 0.12133557627232017 22-23 0.1363337420091144 24-25 0.14802898759057584 26-27 0.1452038483382157 28-29 0.545563471946575 30-31 0.17794222673321256 32-33 0.15829089046311928 34-35 0.14293958231978002 36-37 0.13203371388236038 38-39 0.421278117925467 40-41 0.12056697221101631 42-43 0.12129403010684428 44-45 0.12532400815800504 46-47 0.13577286877518996 48-49 0.15058407676734273 50-51 0.16300638024463213 52-53 0.1808296852337865 54-55 0.1787108307945164 56-57 0.15016861511258386 58-59 0.13465112230734108 60-61 0.11450123205153717 62-63 0.11890512559198092 64-65 0.11418963581046805 66-67 0.1107620771587076 68-69 0.10816544181646484 70-71 0.12216649958183784 72-73 0.13517044937578965 74-75 0.14414442111858064 76-77 0.16433585753986044 78-79 0.16551992325592313 80-81 0.16448126911902602 82-83 0.15721069016074626 84-85 0.14424828653227037 86-87 0.14350045555370444 88-89 0.14059222397039253 90-91 0.14784202984593436 92-93 0.13884728502040541 94-95 0.1903853032932399 96-97 0.1919640575813235 98-99 0.20839556602703577 100-101 0.23531748125540877 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2406961.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.75253176582127 #Duplication Level Percentage of deduplicated Percentage of total 1 84.69468228156778 60.77057880804332 2 9.667446638843577 13.873275440960118 3 2.7541632324419796 5.928545544721505 4 1.0714961293221212 3.075302402245602 5 0.5355098194524232 1.9212092665584606 6 0.30149645162126565 1.2979880233342356 7 0.1933980903083312 0.9713761832808391 8 0.13516275186614246 0.7758615717464964 9 0.1021798618269135 0.6598497403406557 >10 0.5022277924798688 6.358807797433444 >50 0.027913444014366722 1.3503609661187 >100 0.013297818572755693 1.736409158453769 >500 5.430111260104008E-4 0.2533423331058749 >1k 3.620074173390539E-4 0.4707093622441377 >5k 1.2066913911301797E-4 0.5563834014128262 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7120 0.29580869818829636 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5732 0.2381426205077689 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3638 0.15114495000126715 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 6.231924821382648E-5 0.0 16-17 0.0 0.0 0.0 1.2463849642765297E-4 0.0 18-19 0.0 0.0 0.0 1.2463849642765297E-4 0.0 20-21 0.0 0.0 0.0 2.4927699285530594E-4 0.0 22-23 0.0 0.0 0.0 4.362347374967854E-4 0.0 24-25 0.0 0.0 0.0 6.43965564876207E-4 0.0 26-27 0.0 0.0 0.0 0.0012256118815385875 0.0 28-29 0.0 0.0 0.0 0.0022019467702218687 2.077308273794216E-5 30-31 0.0 0.0 0.0 0.005276363015437309 4.154616547588432E-5 32-33 0.0 0.0 0.0 0.01105128001658523 4.154616547588432E-5 34-35 0.0 0.0 0.0 0.021500140633770135 8.309233095176865E-5 36-37 0.0 0.0 0.0 0.0388248916372139 8.309233095176865E-5 38-39 0.0 0.0 0.0 0.06354486009536507 8.309233095176865E-5 40-41 0.0 0.0 0.0 0.09146388329515934 8.309233095176865E-5 42-43 0.0 4.154616547588432E-5 0.0 0.12114861852767868 8.309233095176865E-5 44-45 0.0 4.154616547588432E-5 0.0 0.15681600158872536 8.309233095176865E-5 46-47 2.077308273794216E-5 4.154616547588432E-5 0.0 0.19977473669078977 8.309233095176865E-5 48-49 4.154616547588432E-5 4.154616547588432E-5 0.0 0.24938085826899564 8.309233095176865E-5 50-51 4.154616547588432E-5 4.154616547588432E-5 0.0 0.30224835383705845 8.309233095176865E-5 52-53 4.154616547588432E-5 4.154616547588432E-5 0.0 0.356632284444991 1.038654136897108E-4 54-55 4.154616547588432E-5 4.154616547588432E-5 0.0 0.4171027282951406 1.2463849642765297E-4 56-57 4.154616547588432E-5 4.154616547588432E-5 0.0 0.4959365772856311 1.2463849642765297E-4 58-59 4.154616547588432E-5 4.154616547588432E-5 0.0 0.5809815780147664 1.2463849642765297E-4 60-61 8.309233095176865E-5 4.154616547588432E-5 0.0 0.671967680406953 1.2463849642765297E-4 62-63 8.309233095176865E-5 4.154616547588432E-5 0.0 0.7686248343865979 1.2463849642765297E-4 64-65 8.309233095176865E-5 4.154616547588432E-5 0.0 0.8711192246156045 1.2463849642765297E-4 66-67 8.309233095176865E-5 4.154616547588432E-5 0.0 0.9879678150165292 1.2463849642765297E-4 68-69 8.309233095176865E-5 4.154616547588432E-5 0.0 1.1235537260470776 1.2463849642765297E-4 70-71 8.309233095176865E-5 4.154616547588432E-5 0.0 1.2746571298828688 1.661846619035373E-4 72-73 8.309233095176865E-5 4.154616547588432E-5 0.0 1.4281494382335236 1.661846619035373E-4 74-75 8.309233095176865E-5 4.154616547588432E-5 0.0 1.5844253396710624 1.661846619035373E-4 76-77 8.309233095176865E-5 4.154616547588432E-5 0.0 1.7586491845941832 1.661846619035373E-4 78-79 8.309233095176865E-5 4.154616547588432E-5 0.0 1.9569905785760549 1.661846619035373E-4 80-81 8.309233095176865E-5 4.154616547588432E-5 0.0 2.17932488312025 1.661846619035373E-4 82-83 1.038654136897108E-4 4.154616547588432E-5 0.0 2.4036326305245495 2.077308273794216E-4 84-85 1.2463849642765297E-4 4.154616547588432E-5 0.0 2.631347163497871 2.077308273794216E-4 86-87 1.2463849642765297E-4 8.309233095176865E-5 0.0 2.872127965513359 2.077308273794216E-4 88-89 1.2463849642765297E-4 8.309233095176865E-5 0.0 3.135738385457845 2.2850391011736376E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 13240 0.0 31.562841 1 GGTATCA 9075 0.0 30.865381 1 ATCAACG 16630 0.0 25.486595 3 TATCAAC 16185 0.0 25.469843 2 TCAACGC 16715 0.0 25.24175 4 CAACGCA 16730 0.0 24.682188 5 AACGCAG 17065 0.0 24.145502 6 GTACATG 10020 0.0 23.136425 1 GAGTACT 8440 0.0 22.468504 12-13 TACATGG 10225 0.0 22.286095 2 ACGCAGA 18795 0.0 21.252424 7 CGCAGAG 19085 0.0 20.932169 8 ACATGGG 10775 0.0 20.627794 3 GTACTTT 9435 0.0 20.61997 14-15 AGTACTT 8870 0.0 20.3767 12-13 CAACGCG 385 0.0 20.165514 5 GCAGAGT 18400 0.0 20.024685 9 AACGCGG 370 5.456968E-12 19.652288 6 AGAGTAC 14575 0.0 19.476107 10-11 TACACCG 235 2.063507E-6 18.58338 5 >>END_MODULE