FastQCFastQC Report
Fri 27 May 2016
SRR939442_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939442_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2406961
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99290.4125118770100554No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93680.3892044781780843No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58340.24238032938630913No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28270.11745100980032497No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT27860.11574761701581371No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA105800.032.1185231
GTATCAA146250.026.4802741
GTACATG111500.022.2615321
TATCAAC178250.021.5640072
ATCAACG177500.021.5428073
TCAACGC178350.021.4694184
TACATGG114600.021.3253732
CAACGCA180750.021.193855
AACGCAG184700.020.8176046
GAGTACT96400.020.7769312-13
TACCGTG2108.772695E-720.3188927
ACATGGG117500.020.2285843
GTACTTT103200.019.270114-15
AGTACTT99700.018.66277312-13
ACGCAGA207850.018.3393027
CGCAGAG210050.018.0795088
AGAGTAC165700.017.81029310-11
ACTTTTT119600.017.12317516-17
GCAGAGT214000.017.1033159
GTACCGT2504.646892E-617.067876
Produced by FastQC (version 0.11.5)