##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939442_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2406961 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.843735731488795 31.0 31.0 31.0 28.0 34.0 2 31.090358339831845 31.0 31.0 34.0 28.0 34.0 3 31.093904720516868 31.0 31.0 34.0 28.0 34.0 4 35.01024071432815 35.0 35.0 37.0 32.0 37.0 5 34.5704030933613 35.0 35.0 37.0 30.0 37.0 6 34.6353177305324 35.0 35.0 37.0 30.0 37.0 7 34.47437661017358 35.0 35.0 37.0 30.0 37.0 8 34.373684077141256 35.0 35.0 37.0 30.0 37.0 9 35.866019017341785 37.0 35.0 39.0 30.0 39.0 10-11 35.65880481653006 37.0 35.0 39.0 30.0 39.0 12-13 35.64834307660158 37.0 35.0 39.0 30.0 39.0 14-15 36.47010545663183 38.0 35.0 40.0 30.0 41.0 16-17 36.38346736818752 38.0 35.0 40.0 30.0 41.0 18-19 36.37994051420027 38.0 35.0 40.0 30.0 41.0 20-21 36.25395758385782 38.0 34.0 40.0 29.5 41.0 22-23 36.09849079399292 38.0 34.0 40.0 29.0 41.0 24-25 35.937914864428635 38.0 34.0 40.0 29.0 41.0 26-27 35.46507733195511 38.0 34.0 40.0 27.0 41.0 28-29 35.2617676397748 38.0 33.5 40.0 27.0 41.0 30-31 34.96846916090456 37.0 33.0 40.0 26.5 41.0 32-33 34.74716914814989 37.0 33.0 40.0 25.5 41.0 34-35 35.01307935608429 37.5 33.0 40.0 26.0 41.0 36-37 34.860204423752606 38.0 33.0 40.0 25.5 41.0 38-39 34.67711525030941 37.0 33.0 40.0 25.0 41.0 40-41 34.52450018924279 37.0 33.0 40.0 25.0 41.0 42-43 34.37453452714855 37.0 33.0 40.0 24.5 41.0 44-45 33.98944332708341 37.0 33.0 40.0 24.0 41.0 46-47 33.59732687816712 37.0 32.0 40.0 23.0 41.0 48-49 33.211770153317815 37.0 31.0 39.5 21.5 41.0 50-51 32.96693673059098 37.0 31.0 39.0 20.0 40.0 52-53 32.859960132299605 36.0 31.0 39.0 20.0 40.0 54-55 32.52474593481158 36.0 31.0 39.0 18.5 40.0 56-57 32.03615305773546 35.5 30.0 39.0 16.5 40.0 58-59 31.576702738432406 35.0 29.0 39.0 14.0 40.0 60-61 31.192668680547794 35.0 29.0 38.0 12.5 40.0 62-63 31.035003683067572 35.0 29.0 38.0 11.5 40.0 64-65 30.58203809700282 34.0 28.0 38.0 8.0 40.0 66-67 29.697832245723966 34.0 27.0 37.5 7.5 39.5 68-69 26.221425897636067 31.0 18.5 36.0 2.0 39.0 70-71 24.88196277380481 29.5 12.5 35.0 2.0 38.0 72-73 25.9018386670993 30.5 19.5 35.0 2.0 38.0 74-75 26.652843149515093 31.0 22.5 35.0 2.0 37.5 76-77 26.346841515088943 30.5 23.0 34.0 2.0 36.0 78-79 27.468452334707543 32.0 26.0 35.0 2.0 37.0 80-81 27.48203377620161 32.0 26.0 35.0 2.0 36.5 82-83 27.1485223483056 32.0 25.5 35.0 2.0 36.0 84-85 26.78286145891022 32.0 25.0 34.5 2.0 36.0 86-87 26.32911418174204 32.0 24.5 34.0 2.0 35.0 88-89 25.83772566319105 31.0 23.5 34.0 2.0 35.0 90-91 25.235574028827223 31.0 20.5 34.0 2.0 35.0 92-93 24.65360614484406 31.0 19.0 34.0 2.0 35.0 94-95 23.79573599239871 30.5 11.5 34.0 2.0 35.0 96-97 23.115515373950803 30.0 4.5 34.0 2.0 35.0 98-99 22.2455881088227 30.0 2.0 34.0 2.0 35.0 100-101 20.81757888889766 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.22642074663400535 1101 2 0.20924567347004341 1101 3 0.33959532244012536 1101 4 0.3169282967836793 1101 5 0.4423621124796284 1101 6 0.37515977582879145 1101 7 0.4094469048545335 1101 8 0.6256917712324324 1101 9 0.5874734793165146 1101 10-11 0.6618532620745086 1101 12-13 0.6784160082294619 1101 14-15 0.6073437477397547 1101 16-17 0.8130980948850492 1101 18-19 0.6092179565485836 1101 20-21 0.7718845447613276 1101 22-23 0.6764553590384281 1101 24-25 0.6788616860835575 1101 26-27 0.7538138440171949 1101 28-29 0.5794512631224507 1101 30-31 0.44246286324434436 1101 32-33 0.7537069471259343 1101 34-35 0.579594593967137 1101 36-37 0.8733975938524097 1101 38-39 0.8062746855396767 1101 40-41 0.6963798184578067 1101 42-43 0.48816313262962296 1101 44-45 0.6710748087767158 1101 46-47 0.7241934123739142 1101 48-49 0.8286606265234937 1101 50-51 0.5405214858736258 1101 52-53 0.24421379981713898 1101 54-55 0.30812016219540084 1101 56-57 0.581153393225879 1101 58-59 0.358687478829804 1101 60-61 0.26478963962610536 1101 62-63 -0.03537739936415463 1101 64-65 -0.10437275833323412 1101 66-67 -0.505787961172576 1101 68-69 -7.5667040057488 1101 70-71 -8.386880186379045 1101 72-73 -5.522101060042296 1101 74-75 -3.4313400316170295 1101 76-77 -0.6026041560555981 1101 78-79 -0.3334723884113764 1101 80-81 -0.5715975127807482 1101 82-83 -0.7254719401534047 1101 84-85 -0.8397567665642924 1101 86-87 -0.8391502998904805 1101 88-89 -0.6831522725055272 1101 90-91 -0.810333301886029 1101 92-93 -0.8301200295329316 1101 94-95 -0.790248964984503 1101 96-97 -1.147546596307162 1101 98-99 -1.1668642711453323 1101 100-101 -1.2178369254881325 1102 1 0.27880527818348355 1102 2 0.3109201278108884 1102 3 0.35933217003972473 1102 4 0.23820639400224763 1102 5 0.4073676870346077 1102 6 0.30611003910646417 1102 7 0.3956349531756942 1102 8 0.5417527531057331 1102 9 0.5060102317102277 1102 10-11 0.49078248819265724 1102 12-13 0.48425217059724446 1102 14-15 0.6113770530959215 1102 16-17 0.5065387018643008 1102 18-19 0.5236410214263785 1102 20-21 0.3858399316187402 1102 22-23 0.5747769377858063 1102 24-25 0.4266665223753705 1102 26-27 0.4791989476371086 1102 28-29 0.3738489397648621 1102 30-31 0.33661960219231446 1102 32-33 0.5059578563838727 1102 34-35 0.31717628103208284 1102 36-37 0.47706302033719084 1102 38-39 0.5474495595380304 1102 40-41 0.48103517733805745 1102 42-43 0.24146360323063476 1102 44-45 0.1819890357926397 1102 46-47 0.4018455016837166 1102 48-49 0.4127777381106412 1102 50-51 0.4111673826253508 1102 52-53 0.12265864512846036 1102 54-55 -0.044480252868837056 1102 56-57 0.1141077820918035 1102 58-59 0.12629075424622727 1102 60-61 0.0865214262814149 1102 62-63 0.007404073905124875 1102 64-65 -0.30097770820399816 1102 66-67 -0.24256854084277535 1102 68-69 -7.0898141898552645 1102 70-71 -7.930050834572427 1102 72-73 -2.010817492661168 1102 74-75 -0.3659726329507471 1102 76-77 0.062004727321372854 1102 78-79 -0.3436178696812924 1102 80-81 -0.5605383890924394 1102 82-83 -0.5903647708998925 1102 84-85 -0.7192923038302936 1102 86-87 -0.8274882056116013 1102 88-89 -0.8757765307974168 1102 90-91 -0.9234416486859836 1102 92-93 -0.8275828762723414 1102 94-95 -0.7103722733054134 1102 96-97 -0.8538969957125424 1102 98-99 -0.955952131977714 1102 100-101 -0.8400520490261165 1103 1 0.356077162835998 1103 2 0.18992989658787707 1103 3 0.3162124850673358 1103 4 0.33255979533904423 1103 5 0.38223954857524944 1103 6 0.35038812624623006 1103 7 0.3653876963057314 1103 8 0.3206524195083773 1103 9 0.4223753607153853 1103 10-11 0.5250767295656331 1103 12-13 0.4746697731742202 1103 14-15 0.5558632273032345 1103 16-17 0.4928198416967291 1103 18-19 0.47123786858229977 1103 20-21 0.4515608975343497 1103 22-23 0.4250852565337837 1103 24-25 0.6230859080972166 1103 26-27 0.7639502101630526 1103 28-29 0.5473153509169748 1103 30-31 0.5215472532159424 1103 32-33 0.5525567603355981 1103 34-35 0.48625524899085093 1103 36-37 0.6488584353597346 1103 38-39 0.5501547716656674 1103 40-41 0.6186400735232311 1103 42-43 0.5692454737351511 1103 44-45 0.613543999106561 1103 46-47 0.6144907433271314 1103 48-49 0.5476862496180246 1103 50-51 0.5140230225231761 1103 52-53 0.35756210091457064 1103 54-55 0.11465138286563814 1103 56-57 0.4105754780391173 1103 58-59 0.6552074736769704 1103 60-61 0.43097353455731024 1103 62-63 0.06968955684286726 1103 64-65 0.15361689918714916 1103 66-67 -0.03554498037916076 1103 68-69 -6.599390167314773 1103 70-71 -7.785679698086412 1103 72-73 -1.1270232460719711 1103 74-75 0.21082918999789513 1103 76-77 0.4408170822201676 1103 78-79 0.41454591642799343 1103 80-81 0.1035921913605371 1103 82-83 0.17673580505108433 1103 84-85 -0.15142426136544884 1103 86-87 -0.10693096080314746 1103 88-89 -0.11326201566114946 1103 90-91 -0.35023159470211596 1103 92-93 -0.27919159875577293 1103 94-95 -0.025473512117624608 1103 96-97 0.0541133532686473 1103 98-99 0.3368341161394319 1103 100-101 0.18348072849887487 1104 1 0.2088820583510298 1104 2 0.2670774920240433 1104 3 0.2707588367281808 1104 4 0.12445306547277823 1104 5 0.4326593367488485 1104 6 0.29870026547097694 1104 7 0.3482743837704376 1104 8 0.5031665931435043 1104 9 0.5861879638821392 1104 10-11 0.446296068919672 1104 12-13 0.39116881261822556 1104 14-15 0.6111470720662879 1104 16-17 0.547933925557281 1104 18-19 0.5640693444139728 1104 20-21 0.5234157000876181 1104 22-23 0.5672766150770769 1104 24-25 0.4619330035321809 1104 26-27 0.6060082270516673 1104 28-29 0.4059230554748652 1104 30-31 0.5217554203556176 1104 32-33 0.5904096409767519 1104 34-35 0.41568318808815974 1104 36-37 0.5695164835601005 1104 38-39 0.5785378477438812 1104 40-41 0.6431905938610711 1104 42-43 0.6196992007537503 1104 44-45 0.6155601932031445 1104 46-47 0.6345266638272875 1104 48-49 0.8799461419578734 1104 50-51 0.7285956691209847 1104 52-53 0.5570198261824899 1104 54-55 0.5063474609753911 1104 56-57 0.6093282888289231 1104 58-59 0.6801645933798817 1104 60-61 0.8470953794115808 1104 62-63 0.5569668240043875 1104 64-65 0.4553013594101536 1104 66-67 0.342255107688473 1104 68-69 -6.288709246066496 1104 70-71 -4.287265022209716 1104 72-73 0.46920738178199173 1104 74-75 1.0571558334447921 1104 76-77 1.2735605864319162 1104 78-79 0.7431508818743815 1104 80-81 0.7026676145425981 1104 82-83 0.600946630222424 1104 84-85 0.588217523127021 1104 86-87 0.5717069833323158 1104 88-89 0.47677567312617697 1104 90-91 0.23847534463816444 1104 92-93 0.241058223148638 1104 94-95 0.41677224192616436 1104 96-97 0.18427195769267612 1104 98-99 0.2969325027840881 1104 100-101 0.4370308383700525 1105 1 0.045939308659701084 1105 2 0.19106464632777787 1105 3 0.1855538165946271 1105 4 0.1250666247407679 1105 5 0.3104421408214506 1105 6 0.15991859069333003 1105 7 0.31453537980372204 1105 8 0.40656850297339986 1105 9 0.3617880859184126 1105 10-11 0.3679616695254211 1105 12-13 0.2839774683302707 1105 14-15 0.31441117764659055 1105 16-17 0.4079083103698764 1105 18-19 0.4765962763014642 1105 20-21 0.4455664327241351 1105 22-23 0.5844041200162238 1105 24-25 0.44848435456509606 1105 26-27 0.4309943304274171 1105 28-29 0.5059782096024108 1105 30-31 0.5332244252762521 1105 32-33 0.6627576827079693 1105 34-35 0.4874550975316225 1105 36-37 0.5311651785204887 1105 38-39 0.35402940451439946 1105 40-41 0.5597107278199331 1105 42-43 0.44361613555702917 1105 44-45 0.5915716607217973 1105 46-47 0.562572956543022 1105 48-49 0.7133734480881415 1105 50-51 0.41774547945942686 1105 52-53 0.301952794055552 1105 54-55 0.43578078136466303 1105 56-57 0.4147173145897618 1105 58-59 0.5406419454629123 1105 60-61 0.7319491804389422 1105 62-63 0.3876882405331692 1105 64-65 0.3397845387524079 1105 66-67 0.5101101823140901 1105 68-69 -3.102594939474944 1105 70-71 -3.493012212010985 1105 72-73 0.6454456946331746 1105 74-75 1.1634683192745605 1105 76-77 1.0709621271263927 1105 78-79 0.8160263467728761 1105 80-81 0.5807186227876109 1105 82-83 0.5358775281267576 1105 84-85 0.5802683432913263 1105 86-87 0.6806815137993603 1105 88-89 0.6118866664166127 1105 90-91 0.6181011234774125 1105 92-93 0.6096710117180884 1105 94-95 0.7634301882415535 1105 96-97 0.7878980781037832 1105 98-99 0.9004792428670854 1105 100-101 1.0200534737212799 1106 1 0.1819810645645603 1106 2 0.15820589993498047 1106 3 0.20767711482999118 1106 4 0.19420077256734913 1106 5 0.30942671759820684 1106 6 0.3280918230407366 1106 7 0.358777476010367 1106 8 0.46301940652414686 1106 9 0.6190987308294424 1106 10-11 0.4928517710309279 1106 12-13 0.11621014616604697 1106 14-15 0.32884222903237514 1106 16-17 0.3539311883487528 1106 18-19 0.2498969731741738 1106 20-21 0.2383189756119961 1106 22-23 0.3547392792294275 1106 24-25 0.4684513444461871 1106 26-27 0.6945333408302545 1106 28-29 0.5683294366675966 1106 30-31 0.4890323774610863 1106 32-33 0.5596052886288376 1106 34-35 0.5328971959747619 1106 36-37 0.5990127281198667 1106 38-39 0.6349890053709757 1106 40-41 0.5160841450054576 1106 42-43 0.6131615393350458 1106 44-45 0.7713063442434489 1106 46-47 0.6307203873763498 1106 48-49 0.7886509336896879 1106 50-51 0.7645744911073322 1106 52-53 0.6303726572393415 1106 54-55 0.3731662206647641 1106 56-57 0.7238147484437256 1106 58-59 0.8736582147358014 1106 60-61 0.9088596073189947 1106 62-63 0.358825319531757 1106 64-65 0.5564644811981196 1106 66-67 0.5603510623584604 1106 68-69 -3.0399991980612064 1106 70-71 -3.1319080217605517 1106 72-73 0.7987823745265246 1106 74-75 1.257735010106277 1106 76-77 1.3207085099709523 1106 78-79 0.9710513075558005 1106 80-81 0.7357474701622451 1106 82-83 0.5792510051794366 1106 84-85 0.493570823905074 1106 86-87 0.43550139891171114 1106 88-89 0.4765127224274437 1106 90-91 0.6973966630474813 1106 92-93 0.497911589835649 1106 94-95 0.8481906848688787 1106 96-97 0.9373339836380552 1106 98-99 0.6612572730687667 1106 100-101 0.839008463307259 1107 1 0.043387296648393914 1107 2 0.02968651351515561 1107 3 0.16954380659243995 1107 4 0.009361660376718817 1107 5 0.17291318855788518 1107 6 0.13862344894069878 1107 7 0.06454678047003881 1107 8 0.0030166199524899184 1107 9 0.1435344537385106 1107 10-11 0.05391674351682951 1107 12-13 0.08061440929769503 1107 14-15 0.10730486364631986 1107 16-17 -0.034961901115025285 1107 18-19 0.13697071616682166 1107 20-21 0.24248965709026038 1107 22-23 0.3874529404924303 1107 24-25 0.22056758999747217 1107 26-27 0.3877685050155506 1107 28-29 0.29380536710431926 1107 30-31 0.32660975143882354 1107 32-33 0.3002990128378187 1107 34-35 0.1807935325726504 1107 36-37 0.13814005550548103 1107 38-39 0.27439708939447627 1107 40-41 0.036994737310500625 1107 42-43 -0.040362996663333206 1107 44-45 0.21635061446925619 1107 46-47 0.2570604339787579 1107 48-49 0.22121421110904294 1107 50-51 0.108385901575069 1107 52-53 -0.017966623863344466 1107 54-55 0.027310614700674307 1107 56-57 0.29697282530993974 1107 58-59 0.3990921410549113 1107 60-61 0.45768686145800075 1107 62-63 0.13678121799777898 1107 64-65 0.08289712478400801 1107 66-67 0.5339529205818607 1107 68-69 -2.6825597197403113 1107 70-71 -2.5323791109327516 1107 72-73 1.239836914252571 1107 74-75 1.4301868716403874 1107 76-77 1.138389938469178 1107 78-79 1.0457752527070276 1107 80-81 0.7690357700701753 1107 82-83 0.7726725525430034 1107 84-85 0.6802522593036677 1107 86-87 0.5342654706948551 1107 88-89 0.5371470829701792 1107 90-91 0.39231089081550863 1107 92-93 0.5050510418911252 1107 94-95 0.8107910172314128 1107 96-97 0.9386827841569669 1107 98-99 0.9801957419548124 1107 100-101 1.3073763236533331 1108 1 -0.0522432994977855 1108 2 -0.006819589091552558 1108 3 0.08278636418922503 1108 4 -0.05644689591861152 1108 5 -0.044013052114607376 1108 6 -0.18502841835889683 1108 7 0.13233139591508802 1108 8 0.24292113938514603 1108 9 0.24036633792654527 1108 10-11 0.2236786419115262 1108 12-13 0.059569130060545206 1108 14-15 0.18464282384985609 1108 16-17 0.3727252092047948 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2312 98-99 2.354039776610488 2312 100-101 2.448817006540505 2313 1 -0.35826891557295326 2313 2 -0.2397251561563145 2313 3 -0.14742090515036566 2313 4 -0.020238193270287752 2313 5 -0.14483733898210005 2313 6 0.033693380409012263 2313 7 -3.058348949752606 2313 8 -3.3918818535968605 2313 9 -1.07854869266081 2313 10-11 -0.1130206062347483 2313 12-13 -0.05039537885080847 2313 14-15 -0.7030407012758388 2313 16-17 -0.29353687594080213 2313 18-19 0.08642953478612014 2313 20-21 -2.8262345771996635 2313 22-23 -0.5989076414508006 2313 24-25 -0.12021284447610014 2313 26-27 -1.804037865537076 2313 28-29 -1.7850649786348356 2313 30-31 -1.055464818104781 2313 32-33 -1.0216202585966911 2313 34-35 -1.6607321300844902 2313 36-37 -2.3079240547090905 2313 38-39 -1.8561370317213388 2313 40-41 -1.0099338494763188 2313 42-43 -0.27799258499405965 2313 44-45 -0.23720794910143894 2313 46-47 -0.18602009065872238 2313 48-49 -2.553199832442857 2313 50-51 -0.6277157115944902 2313 52-53 -0.5060804917203683 2313 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0.2215816023412991 2315 96-97 0.35533711367400755 2315 98-99 -2.207548122369438 2315 100-101 -3.6857762498760813 2316 1 -0.49457874090561305 2316 2 -0.42439619053604716 2316 3 -0.9846638770672378 2316 4 -0.29004032081754616 2316 5 -0.15185784057646856 2316 6 -0.38108791757228033 2316 7 -0.615167220915481 2316 8 -0.8282772660379933 2316 9 -5.138598944199302 2316 10-11 -1.3517951605808776 2316 12-13 -0.5816790394630971 2316 14-15 -0.26668600448670077 2316 16-17 -0.24450456062980663 2316 18-19 -0.39549889327522436 2316 20-21 -3.3785906937014616 2316 22-23 -1.038646510914731 2316 24-25 -0.3698180430505502 2316 26-27 -0.28952680716599843 2316 28-29 0.10248709528007538 2316 30-31 0.2678960820649223 2316 32-33 -1.18594947244204 2316 34-35 -0.29732022583871043 2316 36-37 -0.16103911096408297 2316 38-39 -5.296316191822019 2316 40-41 -4.824579072940658 2316 42-43 -3.355183845224918 2316 44-45 -2.1376604263540244 2316 46-47 -2.2367981246487076 2316 48-49 -2.686287505315896 2316 50-51 -1.253190119139628 2316 52-53 -0.8599663373872701 2316 54-55 0.029798712459758292 2316 56-57 -3.4703112311912783 2316 58-59 -0.7119309322055933 2316 60-61 -0.3606994647913879 2316 62-63 -0.09367651594994442 2316 64-65 0.114050403188795 2316 66-67 0.07080831073088945 2316 68-69 2.9038229848856183 2316 70-71 4.126847319668201 2316 72-73 2.9323160110041186 2316 74-75 1.3349648548930304 2316 76-77 -1.024053048149998 2316 78-79 0.24434682906372984 2316 80-81 0.2629634056832195 2316 82-83 0.5996120464087475 2316 84-85 0.6069159315290591 2316 86-87 0.7095720288307099 2316 88-89 0.46741027402186575 2316 90-91 0.5550506321647468 2316 92-93 0.39690002390635115 2316 94-95 0.8368983599934054 2316 96-97 1.064125374218989 2316 98-99 1.2196786880075656 2316 100-101 1.1009669962045443 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 25.0 4 73.0 5 178.0 6 387.0 7 1101.0 8 2307.0 9 4003.0 10 6217.0 11 9187.0 12 12320.0 13 15183.0 14 19068.0 15 24194.0 16 28345.0 17 32267.0 18 30004.0 19 27393.0 20 27382.0 21 27960.0 22 29583.0 23 33085.0 24 39595.0 25 48354.0 26 61236.0 27 75915.0 28 92715.0 29 110299.0 30 129082.0 31 149163.0 32 171193.0 33 196728.0 34 224438.0 35 248353.0 36 248673.0 37 198091.0 38 78603.0 39 4252.0 40 1.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.059724285521355 20.548644802044482 15.83070673911011 22.560924173324054 2 16.078575750121335 21.539643571860726 38.830206304144035 23.551574373873905 3 19.45147269121055 25.09071631614065 30.68857983991385 24.76923115273495 4 13.397283583653596 18.015490775478565 36.770714733036534 31.8165109078313 5 13.414675185846384 37.939210481599 34.190707701537335 14.455406631017286 6 31.260996750674398 36.88335623219487 17.230399661648025 14.625247355482701 7 27.734558225081337 33.39185803176703 20.569132611621043 18.304451131530588 8 26.171134472058334 35.32948809723132 19.383529687435733 19.11584774327461 9 25.850107251426174 17.172899768629403 20.47972526351694 36.49726771642748 10-11 24.903999727434847 27.208338665527656 26.983534737930263 20.904126869107234 12-13 25.95004655247842 24.539325730662025 27.634182689291602 21.87644502756796 14-15 22.69623396473811 25.88668865012769 26.45391844736994 24.96315893776426 16-17 21.765465448747083 29.15485017112972 27.021108907304107 22.058575472819083 18-19 21.463992531655087 27.482541248106013 29.750319801874483 21.30314641836442 20-21 22.54047739036902 26.38445325869426 29.79501952877508 21.28004982216164 22-23 22.146174163856237 26.53094207105859 29.615001511243968 21.707882253841206 24-25 21.993703887436112 26.680202851656208 29.81564945851105 21.51044380239663 26-27 21.9213289622354 26.786921024625133 29.781598137498467 21.510151875641004 28-29 21.58157289795769 27.052207103396732 29.83352673298343 21.53269326566215 30-31 21.81535063055772 26.66996682324753 29.77569391054637 21.73898863564838 32-33 21.798903132166114 26.806413691715786 29.63342112790719 21.761262048210916 34-35 22.198985704174866 26.806588498068983 29.484212312852492 21.510213484903662 36-37 22.101296813434836 26.561868495766273 29.64922252733088 21.687612163468014 38-39 21.866904480064242 26.878306174214394 29.70098181197424 21.553807533747122 40-41 22.085968644029744 26.680764158333183 29.661589518517715 21.571677679119357 42-43 21.852694209927797 26.80779937340571 29.2931583969664 22.04634801970009 44-45 22.098830956807582 26.86784861999766 29.152922358509763 21.880398064684993 46-47 22.234533948383746 27.094406524096875 28.684621875590537 21.986437651928835 48-49 22.330319498129295 27.15176982264979 28.582790664220344 21.935120015000575 50-51 22.063883534864537 27.326970313297732 28.580394070974684 22.028752080863047 52-53 22.38051798213644 27.455584799533263 28.199383652512537 21.964513565817757 54-55 22.040194452682535 27.222294846746202 28.39222397098381 22.345286729587446 56-57 22.242148944247358 27.553835914768488 28.146276684717076 22.057738456267078 58-59 22.41071157721168 27.275630463717608 28.221923394819658 22.091734564251052 60-61 22.269778052018474 27.230314400337395 28.640950368666758 21.858957178977374 62-63 22.18567224887589 27.24451862923405 28.504831417142988 22.064977704747076 64-65 22.021279734019945 27.26549019298262 28.433993394540547 22.279236678456886 66-67 21.861118306281227 27.433446410199597 28.313157382816684 22.392277900702492 68-69 22.015636986218244 26.942497121738228 28.92949168913685 22.11237420290668 70-71 21.59612378501486 26.858009752997557 29.35264090935195 22.193225552635628 72-73 22.033188757618998 27.025345643256298 28.598805311468794 22.342660287655914 74-75 21.907393963175984 27.43648328096087 28.067737865153198 22.588384890709946 76-77 21.320056547269672 27.49288371926512 28.514014847821073 22.673044885644135 78-79 21.93527369853064 27.431329172044183 27.98692027760026 22.64647685182492 80-81 21.83701454598862 27.51844864946725 27.98047765918043 22.664059145363705 82-83 21.89316833667959 27.551733700885052 27.91290104459334 22.64219691784201 84-85 21.78198447251031 27.455642124059548 27.8611541460857 22.90121925734444 86-87 21.8089817990853 27.41992644517124 27.935836457668582 22.835255298074877 88-89 21.774800660752685 27.37025914933077 27.732210696349576 23.12272949356697 90-91 21.637892674317296 27.45935473631423 27.923486233729328 22.97926635563915 92-93 21.74923655400212 27.352057668737146 27.962959885327294 22.93574589193344 94-95 21.4483061710614 27.303561204816575 28.018101436398208 23.23003118772382 96-97 21.376534893719864 27.20984277060876 28.056368256873604 23.357254078797773 98-99 21.4131260847579 27.195973810822792 27.674981291367267 23.71591881305204 100-101 21.234239798758068 27.019758332673703 28.08470094701963 23.661300921548598 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1297.0 1 940.5 2 599.5 3 598.5 4 536.0 5 579.0 6 935.5 7 1840.0 8 2818.0 9 3302.0 10 3271.5 11 3017.5 12 2941.0 13 2990.5 14 3115.0 15 3333.5 16 3621.0 17 3826.5 18 4095.0 19 4496.5 20 5031.0 21 5839.0 22 6761.0 23 7887.0 24 9261.0 25 11246.0 26 14121.5 27 17369.5 28 20421.0 29 23871.0 30 27993.0 31 32877.0 32 38505.5 33 43567.5 34 48577.5 35 53359.5 36 58689.5 37 63815.0 38 66998.5 39 69259.5 40 71764.0 41 75437.5 42 79137.5 43 82830.5 44 86159.0 45 88314.0 46 90000.5 47 93876.0 48 100696.0 49 101040.5 50 94982.5 51 89869.0 52 85203.0 53 81460.0 54 77551.5 55 71579.0 56 64896.0 57 57848.0 58 50689.5 59 43600.5 60 35857.5 61 28492.0 62 22172.0 63 16801.0 64 12447.5 65 9141.5 66 6598.0 67 4876.5 68 3386.5 69 2133.0 70 1459.5 71 1090.0 72 811.0 73 578.5 74 422.5 75 323.5 76 201.5 77 109.0 78 64.5 79 40.5 80 25.0 81 16.0 82 11.0 83 5.5 84 1.5 85 1.0 86 1.5 87 2.0 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02019143642127978 2 0.017324751003443762 3 2.9082315833119023E-4 4 0.0903629099100484 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.008184594598749212 12-13 0.0 14-15 0.0 16-17 6.231924821382648E-4 18-19 8.309233095176865E-5 20-21 0.0 22-23 1.4541157916559512E-4 24-25 0.0032821470725948616 26-27 0.003759927975567531 28-29 0.001163292633324761 30-31 1.8695774464147944E-4 32-33 6.855117303520913E-4 34-35 0.00288745850057396 36-37 0.0826353231315339 38-39 0.09449675337489889 40-41 0.0749908286839712 42-43 0.07877152974227666 44-45 0.07787828718454516 46-47 0.0767149945512204 48-49 0.07145940461852103 50-51 0.07100239679828631 52-53 0.055567996323995276 54-55 0.047009486235963106 56-57 0.026776503649207445 58-59 0.021520913716508078 60-61 0.013128588290379446 62-63 0.0024304506803392327 64-65 0.006813571138045029 66-67 0.1549048779768347 68-69 0.7269748034970238 70-71 0.8906666954720082 72-73 0.3605791701652 74-75 0.052992134064490444 76-77 0.19622253954260163 78-79 0.013917965434421248 80-81 0.003219827824381035 82-83 0.001807258198200968 84-85 1.661846619035373E-4 86-87 0.0020357621083183317 88-89 3.739154892829589E-4 90-91 0.004154616547588432 92-93 0.00461162436782316 94-95 0.07677731379943423 96-97 0.03699686035627499 98-99 0.013211680621331214 100-101 0.012692353552882661 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2406961.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.7373543176321 #Duplication Level Percentage of deduplicated Percentage of total 1 76.54141760186045 44.9584036565432 2 13.738953109977531 16.13979513548167 3 4.484122146083558 7.901564138941524 4 1.9077992440390465 4.482363206561285 5 0.9670548265802276 2.840112099670954 6 0.5644728459083908 1.9893384931681974 7 0.3715189812113501 1.5275429424595741 8 0.24224267597288585 1.1382955111576591 9 0.16992946316973814 0.8983086378485331 >10 0.913643449611691 9.70316736842614 >50 0.06311566368340128 2.550773122799925 >100 0.03315633666625127 3.5568514030260436 >500 0.0018587510034414174 0.7250266752480174 >1k 5.004329624642286E-4 0.5331671316815104 >5k 2.1447126962752656E-4 1.0552904769857476 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9929 0.4125118770100554 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9368 0.3892044781780843 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5834 0.24238032938630913 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2827 0.11745100980032497 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2786 0.11574761701581371 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 4.154616547588432E-5 8 0.0 0.0 0.0 0.0 4.154616547588432E-5 9 0.0 0.0 0.0 0.0 4.154616547588432E-5 10-11 0.0 0.0 0.0 0.0 4.154616547588432E-5 12-13 0.0 0.0 0.0 0.0 8.309233095176865E-5 14-15 0.0 0.0 0.0 2.077308273794216E-5 8.309233095176865E-5 16-17 0.0 0.0 0.0 1.2463849642765297E-4 8.309233095176865E-5 18-19 0.0 0.0 0.0 1.2463849642765297E-4 8.309233095176865E-5 20-21 0.0 0.0 0.0 2.4927699285530594E-4 8.309233095176865E-5 22-23 0.0 0.0 0.0 3.531424065450167E-4 8.309233095176865E-5 24-25 0.0 0.0 0.0 6.024193994003226E-4 8.309233095176865E-5 26-27 0.0 0.0 0.0 0.0012256118815385875 8.309233095176865E-5 28-29 0.0 0.0 0.0 0.0022642660184356957 8.309233095176865E-5 30-31 0.0 0.0 0.0 0.005878782414837631 8.309233095176865E-5 32-33 0.0 0.0 0.0 0.01279621896657237 8.309233095176865E-5 34-35 0.0 0.0 0.0 0.024927699285530593 8.309233095176865E-5 36-37 0.0 0.0 0.0 0.04466212788657564 8.309233095176865E-5 38-39 0.0 0.0 0.0 0.07295506657565287 8.309233095176865E-5 40-41 0.0 2.077308273794216E-5 0.0 0.10338763278673813 8.309233095176865E-5 42-43 0.0 4.154616547588432E-5 0.0 0.1370815729876803 8.309233095176865E-5 44-45 0.0 4.154616547588432E-5 0.0 0.17706975725821897 1.038654136897108E-4 46-47 0.0 4.154616547588432E-5 0.0 0.2253256284584586 1.2463849642765297E-4 48-49 0.0 4.154616547588432E-5 0.0 0.28043661696221917 1.2463849642765297E-4 50-51 0.0 4.154616547588432E-5 0.0 0.34015922983380287 1.2463849642765297E-4 52-53 0.0 4.154616547588432E-5 0.0 0.3995910195470554 1.4541157916559512E-4 54-55 0.0 4.154616547588432E-5 0.0 0.466542665211443 1.661846619035373E-4 56-57 0.0 4.154616547588432E-5 0.0 0.5529586894012823 1.661846619035373E-4 58-59 0.0 4.154616547588432E-5 0.0 0.6378167323857762 1.661846619035373E-4 60-61 0.0 4.154616547588432E-5 0.0 0.7221762213845593 1.661846619035373E-4 62-63 0.0 4.154616547588432E-5 0.0 0.8064110718869146 1.661846619035373E-4 64-65 0.0 4.154616547588432E-5 0.0 0.8990382478153988 1.661846619035373E-4 66-67 0.0 4.154616547588432E-5 0.0 0.9981466255581208 1.661846619035373E-4 68-69 0.0 4.154616547588432E-5 0.0 1.1110067840733606 1.661846619035373E-4 70-71 0.0 4.154616547588432E-5 0.0 1.2561690862461004 1.661846619035373E-4 72-73 0.0 4.154616547588432E-5 0.0 1.4159348655836137 1.661846619035373E-4 74-75 0.0 4.154616547588432E-5 0.0 1.5859417747109321 1.661846619035373E-4 76-77 0.0 4.154616547588432E-5 0.0 1.7833276068868587 1.661846619035373E-4 78-79 0.0 4.154616547588432E-5 0.0 2.009712662564952 1.661846619035373E-4 80-81 0.0 4.154616547588432E-5 0.0 2.2625210794857082 2.077308273794216E-4 82-83 0.0 4.154616547588432E-5 0.0 2.5231817216814063 2.9082315833119023E-4 84-85 0.0 4.154616547588432E-5 0.0 2.787747703431838 2.9082315833119023E-4 86-87 0.0 4.154616547588432E-5 0.0 3.0687244205452435 2.9082315833119023E-4 88-89 0.0 4.154616547588432E-5 0.0 3.3766022798042843 3.323693238070746E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10580 0.0 32.118523 1 GTATCAA 14625 0.0 26.480274 1 GTACATG 11150 0.0 22.261532 1 TATCAAC 17825 0.0 21.564007 2 ATCAACG 17750 0.0 21.542807 3 TCAACGC 17835 0.0 21.469418 4 TACATGG 11460 0.0 21.325373 2 CAACGCA 18075 0.0 21.19385 5 AACGCAG 18470 0.0 20.817604 6 GAGTACT 9640 0.0 20.77693 12-13 TACCGTG 210 8.772695E-7 20.318892 7 ACATGGG 11750 0.0 20.228584 3 GTACTTT 10320 0.0 19.2701 14-15 AGTACTT 9970 0.0 18.662773 12-13 ACGCAGA 20785 0.0 18.339302 7 CGCAGAG 21005 0.0 18.079508 8 AGAGTAC 16570 0.0 17.810293 10-11 ACTTTTT 11960 0.0 17.123175 16-17 GCAGAGT 21400 0.0 17.103315 9 GTACCGT 250 4.646892E-6 17.06787 6 >>END_MODULE