FastQCFastQC Report
Fri 27 May 2016
SRR939441_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939441_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1859933
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121100.6510987223733328No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92330.49641573110429243No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63110.3393132978445998No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35640.19161980565966624No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21080.11333741591767015No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT18900.10161656360739876No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA115850.033.32621
GTATCAA175500.032.9133721
GTACATG138150.027.4051251
TACATGG140400.026.3348462
TATCAAC215950.026.2576522
CAACGCA223400.025.7800665
ATCAACG220600.025.7149333
TCAACGC223100.025.6853524
AACGCAG227150.025.3622026
GAGTACT110400.024.8952412-13
ACATGGG142300.024.8269863
GTCAACG3500.024.6193753
CATGGGA100650.023.3343644
ACGCAGA245850.022.9327327
CGCAGAG246800.022.8256058
GTACTTT123750.022.32333614-15
AGTACTT117250.022.31777212-13
GCAGAGT241900.022.1206059
GCAACGC3057.2759576E-1221.9561634
CATGGGG37800.021.7653144
Produced by FastQC (version 0.11.5)