FastQCFastQC Report
Fri 27 May 2016
SRR939441_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939441_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1859933
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170600.9172373413450915No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147660.7938995651993916No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102400.550557466317335No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA49570.26651497661474904No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34500.1854905526166803No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30790.1655435975381909No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT28220.15172589550268747No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19380.10419730173076126No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA132800.032.9405861
GTATCAA191800.028.713441
GTACATG147200.024.5343671
TACATGG149950.023.921242
ACATGGG149550.022.8731863
GAGTACT132050.022.78362812-13
TATCAAC243400.022.524082
ATCAACG243400.022.4626583
TCAACGC244750.022.2806844
CAACGCA247250.022.0170755
AACGCAG252400.021.5866096
GTACTTT142100.021.15558814-15
AGTACTT138000.020.73697712-13
CATGGGA103900.020.5653884
CATGGGG42250.019.9603984
CATTCCG2902.5211193E-919.6046529
ACGCAGA278150.019.4860087
CGCAGAG278000.019.4453958
GTCGGGA6350.019.4014362
ACTTTTT161300.019.04860516-17
Produced by FastQC (version 0.11.5)