##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939441_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1859933 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.971056484292713 31.0 31.0 31.0 30.0 34.0 2 31.207478979081504 31.0 31.0 34.0 30.0 34.0 3 31.181823216212628 31.0 31.0 34.0 28.0 34.0 4 35.13893618748632 35.0 35.0 37.0 33.0 37.0 5 34.778339864930615 35.0 35.0 37.0 32.0 37.0 6 34.75277496554983 35.0 35.0 37.0 32.0 37.0 7 34.61270002736658 35.0 35.0 37.0 30.0 37.0 8 34.634897063496375 35.0 35.0 37.0 30.0 37.0 9 35.945864716632265 37.0 35.0 39.0 30.0 39.0 10-11 35.81520570902285 37.0 35.0 39.0 30.0 39.0 12-13 35.77904472903056 37.0 35.0 39.0 30.0 39.0 14-15 36.5553374772102 38.0 35.0 40.0 30.0 41.0 16-17 36.58406969498364 38.0 35.0 40.0 30.5 41.0 18-19 36.636997139144256 38.0 35.0 40.0 30.0 41.0 20-21 36.572557721165225 38.0 35.0 40.0 30.0 41.0 22-23 36.421496096902416 38.0 35.0 40.0 30.0 41.0 24-25 36.2091540394197 38.0 34.0 40.0 29.5 41.0 26-27 35.82286593119214 38.0 34.0 40.0 28.0 41.0 28-29 35.54612370445602 38.0 34.0 40.0 27.0 41.0 30-31 35.27221222484896 37.5 34.0 40.0 27.0 41.0 32-33 34.97795216279296 37.0 33.0 40.0 27.0 41.0 34-35 34.85973957126413 37.0 33.0 40.0 26.0 41.0 36-37 34.76772604174452 37.5 33.0 40.0 25.0 41.0 38-39 34.70260272816279 37.5 33.0 40.0 25.0 41.0 40-41 34.44594859062127 37.0 33.0 40.0 24.5 41.0 42-43 34.081553744140244 37.0 33.0 40.0 24.0 41.0 44-45 33.786924851594115 37.0 33.0 40.0 23.5 41.0 46-47 33.32274092668929 37.0 32.0 40.0 21.0 41.0 48-49 32.91493887145397 37.0 31.5 40.0 18.5 41.0 50-51 32.62754276632545 37.0 31.0 40.0 16.0 41.0 52-53 32.26768759949955 36.0 31.0 39.0 13.5 41.0 54-55 32.05181530732558 36.0 31.0 39.0 12.5 40.5 56-57 31.669078133459646 35.5 30.0 39.0 9.0 40.0 58-59 31.25583018313025 35.0 29.5 39.0 8.0 40.0 60-61 30.9747851132272 35.0 29.0 39.0 7.5 40.0 62-63 30.611727680513223 35.0 28.0 38.0 6.5 40.0 64-65 30.193620684185937 34.5 28.0 38.0 2.0 40.0 66-67 28.504263863268193 33.5 25.0 37.5 2.0 39.5 68-69 24.860893913920556 29.5 13.0 35.0 2.0 39.0 70-71 24.269976660449597 28.5 10.5 35.0 2.0 38.0 72-73 26.109340497749113 31.0 20.0 35.0 2.0 38.0 74-75 26.713543713671406 31.5 22.0 35.0 2.0 38.0 76-77 26.75694124465774 31.5 24.5 34.5 2.0 36.5 78-79 27.56848821973695 32.5 26.0 35.0 2.0 37.0 80-81 27.422461454256684 33.0 26.0 35.0 2.0 37.0 82-83 27.017813007242733 32.5 25.0 35.0 2.0 36.0 84-85 26.677472521859656 32.0 25.0 35.0 2.0 36.0 86-87 26.23950513271177 32.0 24.0 35.0 2.0 35.5 88-89 25.747381760525784 32.0 22.5 34.0 2.0 35.0 90-91 25.300755726147123 31.5 20.0 34.0 2.0 35.0 92-93 24.748904933672343 31.0 19.0 34.0 2.0 35.0 94-95 23.38682011663861 31.0 6.5 34.0 2.0 35.0 96-97 22.60733478033886 30.0 2.0 34.0 2.0 35.0 98-99 22.310647211485573 30.0 2.0 34.0 2.0 35.0 100-101 21.114296321426632 28.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3298534493065972 1101 2 0.2619133899730848 1101 3 0.3479883264405679 1101 4 0.24413517787395733 1101 5 0.48176885414024895 1101 6 0.5849515670181091 1101 7 0.4886693156761055 1101 8 0.5377883290795822 1101 9 0.6852763132698243 1101 10-11 0.835658510319611 1101 12-13 0.6691293405576957 1101 14-15 0.8107466759048094 1101 16-17 0.746399093307204 1101 18-19 0.9123177879425128 1101 20-21 0.8208077985618516 1101 22-23 0.9531804182063723 1101 24-25 0.900565538388669 1101 26-27 0.831476162941506 1101 28-29 0.7558704444125297 1101 30-31 1.111128567551006 1101 32-33 0.9408916119521464 1101 34-35 0.9245443521970031 1101 36-37 0.9542570185962589 1101 38-39 0.8403963887460293 1101 40-41 0.9926167812529485 1101 42-43 1.0208384803180408 1101 44-45 0.8016020045804524 1101 46-47 0.8983384452300882 1101 48-49 0.8348598399187637 1101 50-51 0.9143155133698642 1101 52-53 0.9615951513214114 1101 54-55 0.849222963168426 1101 56-57 0.7658753873800457 1101 58-59 0.7088275601265153 1101 60-61 0.44969694618750466 1101 62-63 0.48719025994802934 1101 64-65 0.3294688567131878 1101 66-67 -2.513430359428451 1101 68-69 -7.328541523486187 1101 70-71 -10.914378717284276 1101 72-73 -10.785898644577353 1101 74-75 -9.252731468159904 1101 76-77 -4.156264985602149 1101 78-79 -1.187777767779064 1101 80-81 -0.568218418369387 1101 82-83 -0.26917963206189555 1101 84-85 -0.3404397861402586 1101 86-87 -0.18063193230759467 1101 88-89 -0.1384378449334953 1101 90-91 -0.15866435476513274 1101 92-93 -0.7323375581959404 1101 94-95 -4.422954917417087 1101 96-97 -1.4360667794271151 1101 98-99 -0.5766435262457783 1101 100-101 -0.3280083413245194 1102 1 0.36840793576889297 1102 2 0.2140809425469996 1102 3 0.3693326396759815 1102 4 0.2581584070797689 1102 5 0.42308365589827446 1102 6 0.3795696277045124 1102 7 0.33507263075650684 1102 8 0.5611895796231607 1102 9 0.6028563299043341 1102 10-11 0.47318505587241333 1102 12-13 0.6307008795031308 1102 14-15 0.5237661349032692 1102 16-17 0.6606614963605253 1102 18-19 0.6737559130559845 1102 20-21 0.803740221922773 1102 22-23 0.6408301393461358 1102 24-25 0.45524209428526774 1102 26-27 0.6274336393935371 1102 28-29 0.5882180212204773 1102 30-31 0.3363076840643444 1102 32-33 0.4876620261683158 1102 34-35 0.556141265102255 1102 36-37 0.9270417579115389 1102 38-39 0.6248917804802829 1102 40-41 0.5917332312628645 1102 42-43 0.782628188876366 1102 44-45 0.4889490114242463 1102 46-47 0.6302740646122515 1102 48-49 0.4667595726689058 1102 50-51 0.534328010361719 1102 52-53 0.7401694598704509 1102 54-55 0.6104334192664034 1102 56-57 0.36434366148636954 1102 58-59 0.27720415444292 1102 60-61 0.26330853461427495 1102 62-63 0.268137097069161 1102 64-65 0.14725807130257706 1102 66-67 -2.992266640391229 1102 68-69 -7.612485316576532 1102 70-71 -10.676043878119716 1102 72-73 -9.03431551636075 1102 74-75 -7.5339095629597175 1102 76-77 -4.013643066047312 1102 78-79 -1.3039150362575107 1102 80-81 -0.8446507171825921 1102 82-83 -0.5842246803399718 1102 84-85 -0.5297991886489442 1102 86-87 -0.38201493977623713 1102 88-89 -0.4175302931195297 1102 90-91 -0.3481021801196711 1102 92-93 -0.6283091782029508 1102 94-95 -4.076006864372424 1102 96-97 -1.047254648056711 1102 98-99 -0.22372425427999687 1102 100-101 -0.02275116083164974 1103 1 0.33242650825811637 1103 2 0.23775152026415114 1103 3 0.32974953589915756 1103 4 0.2253176262189598 1103 5 0.3320954363026516 1103 6 0.36727090476700397 1103 7 0.45972646651246407 1103 8 0.35989066788304314 1103 9 0.6268010163864233 1103 10-11 0.6750335441866184 1103 12-13 0.5496450777747199 1103 14-15 0.7568285236053924 1103 16-17 0.5823479322419658 1103 18-19 0.6484308558140839 1103 20-21 0.7250620157858734 1103 22-23 0.7574564145216414 1103 24-25 0.6511623271710789 1103 26-27 0.7890650820733427 1103 28-29 0.7615161267337456 1103 30-31 0.7917543464921906 1103 32-33 0.7410295809320502 1103 34-35 0.7882970667679459 1103 36-37 0.8033784563080602 1103 38-39 0.754696498370933 1103 40-41 0.8607948195924848 1103 42-43 0.90697791235268 1103 44-45 0.5824674577314397 1103 46-47 0.5941638415261821 1103 48-49 0.6422863016188245 1103 50-51 0.6247373816615891 1103 52-53 0.6549045936516293 1103 54-55 0.7032297949254591 1103 56-57 0.6452871643454863 1103 58-59 0.8363845713265974 1103 60-61 0.5833026049998047 1103 62-63 0.5766984693107453 1103 64-65 0.5068119326294713 1103 66-67 -2.476159248298817 1103 68-69 -7.488455386659307 1103 70-71 -11.238869774310064 1103 72-73 -8.681432680399265 1103 74-75 -7.0678155208632205 1103 76-77 -3.6722814751877806 1103 78-79 -0.9764652492824659 1103 80-81 -0.353742200303369 1103 82-83 0.006276374173801003 1103 84-85 -0.018116321826703086 1103 86-87 0.03804858822310919 1103 88-89 0.049387538857860847 1103 90-91 0.15026121701416528 1103 92-93 0.22033803963490328 1103 94-95 1.1646169327427671 1103 96-97 1.2371879755007917 1103 98-99 0.8964683620118521 1103 100-101 1.012531613408317 1104 1 0.35207161315508273 1104 2 0.2139845929466766 1104 3 0.294000616151159 1104 4 0.052048290479504544 1104 5 0.35085335664331296 1104 6 0.39992159473441546 1104 7 0.487501578314955 1104 8 0.4265331457007733 1104 9 0.46142632508685466 1104 10-11 0.5868149677890457 1104 12-13 0.4639953784151629 1104 14-15 0.588104173272832 1104 16-17 0.5348667468748758 1104 18-19 0.6408709538321204 1104 20-21 0.5546684220712947 1104 22-23 0.47277412724962176 1104 24-25 0.5777516888301975 1104 26-27 0.8315900361340383 1104 28-29 0.8262021806564377 1104 30-31 0.6353266209639727 1104 32-33 0.5587868915860312 1104 34-35 0.6756787870207148 1104 36-37 0.6696304367257326 1104 38-39 0.7689264577145138 1104 40-41 0.8514392679856471 1104 42-43 0.8863859310625131 1104 44-45 0.6170404163717009 1104 46-47 0.7733685249413256 1104 48-49 0.6641214904354911 1104 50-51 0.6063143101814177 1104 52-53 0.5855756639656349 1104 54-55 0.5917766232247139 1104 56-57 0.6078774070178063 1104 58-59 0.5795003788103159 1104 60-61 0.40975108966772567 1104 62-63 0.5547637940992445 1104 64-65 0.4029729282169576 1104 66-67 -2.6064384379794703 1104 68-69 -7.144462822070292 1104 70-71 -8.54013500716675 1104 72-73 -7.64537966213101 1104 74-75 -6.697227955279342 1104 76-77 -3.4842820880665357 1104 78-79 -0.7532500192454457 1104 80-81 -0.07165985961726307 1104 82-83 -0.0076660854490882 1104 84-85 0.13486906803760235 1104 86-87 0.2685994236428151 1104 88-89 0.14503120519219692 1104 90-91 -0.030673572048076636 1104 92-93 0.11713819540825199 1104 94-95 0.9119273419989398 1104 96-97 0.9404801771506719 1104 98-99 0.6099881906611664 1104 100-101 0.6161250407092993 1105 1 0.48555325321443377 1105 2 0.330005518273385 1105 3 0.4250753073215847 1105 4 0.3355806572492668 1105 5 0.4763894508206903 1105 6 0.4868888525423074 1105 7 0.5439235351592586 1105 8 0.5698546502505337 1105 9 0.815436863728543 1105 10-11 0.8982263547064733 1105 12-13 0.8651882382396039 1105 14-15 1.0195708232540568 1105 16-17 1.0158678061022144 1105 18-19 0.9868695772835068 1105 20-21 1.0638640349929673 1105 22-23 1.0074047161263948 1105 24-25 1.0726771183312422 1105 26-27 1.1353345388781904 1105 28-29 1.142938866570951 1105 30-31 0.9900744846320322 1105 32-33 1.1904552193476192 1105 34-35 1.2492204499629551 1105 36-37 1.0860961704976475 1105 38-39 0.8060461191193085 1105 40-41 1.091882311051954 1105 42-43 1.1384093011038061 1105 44-45 1.1182816565168636 1105 46-47 0.9493657405588962 1105 48-49 0.6826257899152566 1105 50-51 0.6991179330070949 1105 52-53 1.001959497564151 1105 54-55 0.8772877786316933 1105 56-57 1.0681934768821186 1105 58-59 0.9342512592651104 1105 60-61 1.011593787703017 1105 62-63 1.0625574950485266 1105 64-65 1.3028331504312334 1105 66-67 1.8791579695861849 1105 68-69 -4.723159056226574 1105 70-71 -4.924961128517555 1105 72-73 -1.414581062813923 1105 74-75 1.0611998295500165 1105 76-77 1.2230332845848402 1105 78-79 1.1760602986121391 1105 80-81 1.147073268723549 1105 82-83 1.1670729066312902 1105 84-85 1.2605927616572075 1105 86-87 1.2588893927151652 1105 88-89 1.3791601136070213 1105 90-91 1.32975132560464 1105 92-93 1.4547540623657227 1105 94-95 2.1233320249458245 1105 96-97 2.033239106839428 1105 98-99 1.879331156855276 1105 100-101 1.5920232610285865 1106 1 0.0920790252936392 1106 2 0.09219369410659795 1106 3 0.1463460848900695 1106 4 0.029452307227785468 1106 5 0.23227964134828483 1106 6 0.4217006656938125 1106 7 0.25749187945279317 1106 8 0.24932152914489336 1106 9 0.347754083545766 1106 10-11 0.2837010810685996 1106 12-13 0.18139419089593645 1106 14-15 0.1834203325234114 1106 16-17 0.26680123618641005 1106 18-19 0.3343001718515026 1106 20-21 0.358128471432984 1106 22-23 0.5161603706358662 1106 24-25 0.49762647100006063 1106 26-27 0.6090941956054081 1106 28-29 0.7429741154611591 1106 30-31 0.6215550252251703 1106 32-33 0.7832501029344883 1106 34-35 0.9526120541179139 1106 36-37 0.702555313912903 1106 38-39 0.6304779602496211 1106 40-41 0.6574202329830072 1106 42-43 0.6489751329959716 1106 44-45 0.6949648598699198 1106 46-47 0.7921133502435254 1106 48-49 0.32375766482575585 1106 50-51 0.3901282497330598 1106 52-53 0.6794567070270077 1106 54-55 0.8541458265775184 1106 56-57 0.7793178063270538 1106 58-59 0.8336689182247561 1106 60-61 0.62239509411501 1106 62-63 0.7419999337943999 1106 64-65 0.6757309534340017 1106 66-67 1.3643175213010785 1106 68-69 -5.061379845544693 1106 70-71 -5.7286051890795555 1106 72-73 -2.203152551051442 1106 74-75 0.3617466463182133 1106 76-77 0.3663530691259993 1106 78-79 0.3792190902728443 1106 80-81 0.4231973778629232 1106 82-83 0.401377512119101 1106 84-85 0.3735864526566246 1106 86-87 0.3738494256141287 1106 88-89 0.31272295485116075 1106 90-91 0.29543529176570615 1106 92-93 0.29414637241642794 1106 94-95 1.3330905746375805 1106 96-97 1.2452886049099199 1106 98-99 0.9286991093720864 1106 100-101 0.7988870874057703 1107 1 -0.017104067681199098 1107 2 -0.049505834626330625 1107 3 0.06535373081323925 1107 4 -0.029999210475203597 1107 5 0.12341193916034854 1107 6 0.13803370324492192 1107 7 0.1491909356901644 1107 8 0.14216189840962556 1107 9 0.2522001250252046 1107 10-11 0.2264251691757977 1107 12-13 0.291096320244435 1107 14-15 0.15279305214171757 1107 16-17 0.0017090192277890992 1107 18-19 0.19270837210646619 1107 20-21 0.24042262165443873 1107 22-23 0.26544182301162067 1107 24-25 0.34120197248322626 1107 26-27 0.27787988673883746 1107 28-29 0.33052978725061877 1107 30-31 0.39640415739257406 1107 32-33 0.3074734147314757 1107 34-35 0.2532528289728404 1107 36-37 0.36188530575763167 1107 38-39 0.23858766143398213 1107 40-41 0.40380304321650584 1107 42-43 0.4700134230912383 1107 44-45 0.49088256909804073 1107 46-47 0.4016319150666092 1107 48-49 0.5795629815348136 1107 50-51 0.4154627529654036 1107 52-53 0.6497262232199006 1107 54-55 0.5408372094869769 1107 56-57 0.6998428717440639 1107 58-59 0.7293760361813852 1107 60-61 0.574790221362786 1107 62-63 0.7863859305379606 1107 64-65 0.775480554411498 1107 66-67 1.4537940056695113 1107 68-69 -5.144000419324403 1107 70-71 -5.967505913982244 1107 72-73 -2.1014066563291216 1107 74-75 0.48965556523891607 1107 76-77 0.5205951106470188 1107 78-79 0.39419353771290844 1107 80-81 0.45795450569471186 1107 82-83 0.4875298720926793 1107 84-85 0.5283274494899572 1107 86-87 0.8061306530819969 1107 88-89 0.5892932702230489 1107 90-91 0.7125208257471165 1107 92-93 0.8311783301261855 1107 94-95 1.6297541181085862 1107 96-97 1.7010004147474653 1107 98-99 1.228203178031361 1107 100-101 0.9224922805099816 1108 1 0.018966952181568075 1108 2 -0.050663448750544404 1108 3 -0.07683813829415342 1108 4 -0.11099983322435492 1108 5 -0.07363666456802065 1108 6 0.026318270311413983 1108 7 -0.0854614635005504 1108 8 -0.0893028094794488 1108 9 0.11543085122252705 1108 10-11 0.09418689155441484 1108 12-13 0.08808009758184454 1108 14-15 0.015551164654240779 1108 16-17 -0.04200107261589636 1108 18-19 0.03463687218820155 1108 20-21 0.20973982304433036 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-0.23792612865517526 2311 2 -0.1107788680208941 2311 3 -0.22604678046053905 2311 4 -0.18708233184206335 2311 5 -0.08406155527460157 2311 6 -0.1136643344599193 2311 7 -0.2273738025959844 2311 8 -0.17586231153190823 2311 9 -0.008574512219453823 2311 10-11 -1.3634367315621674 2311 12-13 -0.2420055705319939 2311 14-15 0.07035733917872733 2311 16-17 -0.18385973560132385 2311 18-19 0.0028676502781337376 2311 20-21 -0.0574494300604087 2311 22-23 0.06483417987305984 2311 24-25 -0.14952267614908976 2311 26-27 0.053586453477954876 2311 28-29 -0.1691884481098569 2311 30-31 -0.008013241667036652 2311 32-33 -0.004811470338353274 2311 34-35 0.2313212166298939 2311 36-37 0.01532757034713228 2311 38-39 0.011974658647730507 2311 40-41 0.10486220155173243 2311 42-43 -0.03122325494889111 2311 44-45 0.04653973257155286 2311 46-47 0.1898720860304337 2311 48-49 0.22597203887112016 2311 50-51 0.17433022407588084 2311 52-53 0.5603584083512416 2311 54-55 0.5325032021179901 2311 56-57 0.5359574890230796 2311 58-59 0.5898445370764307 2311 60-61 0.7093816599748131 2311 62-63 0.460658557382601 2311 64-65 0.5951546439844506 2311 66-67 1.7395294246819972 2311 68-69 4.133167651819708 2311 70-71 4.585554438460356 2311 72-73 2.6597758611727436 2311 74-75 1.748893667268046 2311 76-77 0.9875147712736165 2311 78-79 0.7628228365526439 2311 80-81 0.3372461616300164 2311 82-83 0.44765791403330724 2311 84-85 0.3895829756954008 2311 86-87 0.48953043214020653 2311 88-89 0.6966283035452427 2311 90-91 0.5893186384659543 2311 92-93 0.7740024579063203 2311 94-95 1.3861421136046133 2311 96-97 1.86526786925538 2311 98-99 1.4203735799197936 2311 100-101 1.3150164979013788 2312 1 -0.04060781078302611 2312 2 0.008116440537818903 2312 3 0.09762644295009082 2312 4 0.03268071723585564 2312 5 0.13593687551399114 2312 6 0.16802997981973533 2312 7 0.25179324663551483 2312 8 0.2020530411984396 2312 9 -1.3088890222498861 2312 10-11 -0.7895393947142466 2312 12-13 -1.7855207209342225 2312 14-15 -1.7849375807497552 2312 16-17 0.21960204491942648 2312 18-19 -1.6190876205193376 2312 20-21 -1.228936691903371 2312 22-23 -0.21968680165198862 2312 24-25 -0.8218291832013591 2312 26-27 -1.1126184922089735 2312 28-29 0.16465460070096327 2312 30-31 0.7718309890604544 2312 32-33 0.4040240245558735 2312 34-35 0.8852312240252189 2312 36-37 1.0085837173989276 2312 38-39 0.8999258695280403 2312 40-41 1.0607106023750674 2312 42-43 1.0851671581710534 2312 44-45 1.0517836531293838 2312 46-47 1.0347087962287986 2312 48-49 1.33592534648497 2312 50-51 1.282714320285237 2312 52-53 1.533697256182812 2312 54-55 1.475494503010946 2312 56-57 1.5913118386600722 2312 58-59 1.466050033656714 2312 60-61 1.7769124223611463 2312 62-63 1.3247727792791082 2312 64-65 1.6738844007399898 2312 66-67 2.7069732961602426 2312 68-69 4.752550417538114 2312 70-71 5.777148473453767 2312 72-73 3.7760869790355436 2312 74-75 3.004587484074303 2312 76-77 1.8357198605266873 2312 78-79 1.7893779834825843 2312 80-81 1.7641309264235936 2312 82-83 1.7630205771519982 2312 84-85 1.5746405774395633 2312 86-87 1.75290837670698 2312 88-89 2.02685173253926 2312 90-91 1.9698428051562686 2312 92-93 2.0355841562270953 2312 94-95 2.595290866498889 2312 96-97 2.8801766296284548 2312 98-99 2.776276986610977 2312 100-101 2.8166800289275287 2313 1 -0.1994920919287786 2313 2 -0.31619113712392277 2313 3 -0.12301514839901628 2313 4 0.0632688580784091 2313 5 -0.08795775504141545 2313 6 0.08152054595457514 2313 7 -3.377816254853336 2313 8 -1.116837436212471 2313 9 -0.7377945991973078 2313 10-11 -0.12380705690045346 2313 12-13 -1.452823739915182 2313 14-15 -0.45476079162965277 2313 16-17 -0.18973280351395516 2313 18-19 0.228926847039304 2313 20-21 0.2500474705664075 2313 22-23 -1.3220345376993237 2313 24-25 -2.4750712091124427 2313 26-27 -1.9221785470901764 2313 28-29 -0.4998173838433715 2313 30-31 -0.20482597149651127 2313 32-33 -2.1960268733364714 2313 34-35 -2.6812154855212427 2313 36-37 -1.2476490871933024 2313 38-39 -0.575922334327025 2313 40-41 -0.4018797040724422 2313 42-43 -0.4244280466196315 2313 44-45 -0.16435406658327878 2313 46-47 -0.38028194539826643 2313 48-49 -0.4601897393621215 2313 50-51 -0.30916692551402036 2313 52-53 -0.0712259955595087 2313 54-55 0.05738684444779807 2313 56-57 0.3038632897903746 2313 58-59 -0.22625805452786807 2313 60-61 0.3090787066503218 2313 62-63 0.1384715687429896 2313 64-65 -0.02364470986951872 2313 66-67 1.309818431731852 2313 68-69 4.180119989141513 2313 70-71 4.1322189122255075 2313 72-73 2.352889788558034 2313 74-75 1.4727194336437037 2313 76-77 0.8913164157326321 2313 78-79 0.47033157016287674 2313 80-81 0.6851946769343726 2313 82-83 0.38981036628342025 2313 84-85 0.460165093343484 2313 86-87 0.6099107738999265 2313 88-89 0.480221492089175 2313 90-91 0.5462833081447833 2313 92-93 0.9523323933279002 2313 94-95 1.4197376542706337 2313 96-97 1.3999408627685916 2313 98-99 1.0245411060156862 2313 100-101 0.9014211598797246 2314 1 -0.26861107508138105 2314 2 -0.16098900993882026 2314 3 -0.33909020924839695 2314 4 -0.22010358823958853 2314 5 -1.4336664266983092 2314 6 -2.9977111858260663 2314 7 -0.8899031944239013 2314 8 -0.29344872330278093 2314 9 0.016158919242421632 2314 10-11 0.19669917186114105 2314 12-13 0.02050927269669245 2314 14-15 -2.6012222566953227 2314 16-17 -2.8329284220578757 2314 18-19 -0.4164814990351928 2314 20-21 -0.13690347558696914 2314 22-23 0.061060484553230765 2314 24-25 -1.9855778437871479 2314 26-27 -1.1223969383613408 2314 28-29 0.05342889365787329 2314 30-31 0.1674081190902612 2314 32-33 0.18131570880203896 2314 34-35 -0.058448070220528336 2314 36-37 0.2552396610458558 2314 38-39 -1.0986682512671848 2314 40-41 -3.681812663264143 2314 42-43 -5.244612802227373 2314 44-45 -2.4533038090289097 2314 46-47 -3.1474018315822327 2314 48-49 -0.9927527398316798 2314 50-51 -0.9024542754075995 2314 52-53 -2.481856709350179 2314 54-55 -1.5334860741826581 2314 56-57 -3.5476589595094232 2314 58-59 -5.011755280537777 2314 60-61 -2.663329475555546 2314 62-63 -1.6420710117919235 2314 64-65 -0.5739283228569363 2314 66-67 -0.26876408964999854 2314 68-69 1.7320786200818183 2314 70-71 1.5238015273181595 2314 72-73 -0.5315800764031984 2314 74-75 -0.05843289351624392 2314 76-77 -0.08011406713858804 2314 78-79 0.19907863668902692 2314 80-81 0.5340519313281327 2314 82-83 0.2156368128750259 2314 84-85 -0.04811854069128074 2314 86-87 -0.11591948125905205 2314 88-89 0.08578220090365463 2314 90-91 0.342119040624997 2314 92-93 0.4987157326497069 2314 94-95 0.5283522327649521 2314 96-97 0.5545859869754928 2314 98-99 0.11137067914042831 2314 100-101 -0.22771858998759953 2315 1 -0.2724739619008574 2315 2 -0.26186349957323785 2315 3 -0.30913868191544935 2315 4 -0.18025936474803927 2315 5 -1.3990516409263947 2315 6 -0.5591409943931325 2315 7 -0.1914721544168998 2315 8 -0.10778502607487184 2315 9 0.08762811587262576 2315 10-11 -1.5648541023922462 2315 12-13 -1.6306889042060249 2315 14-15 -0.2094368103337274 2315 16-17 -1.8405412815334685 2315 18-19 -3.4141932983400807 2315 20-21 -2.257245846771532 2315 22-23 -1.8406784579010775 2315 24-25 -1.238122755315203 2315 26-27 -0.4479020925229591 2315 28-29 -0.32983619791519203 2315 30-31 -3.361596344718656 2315 32-33 -3.0558053210265967 2315 34-35 -1.632422931860468 2315 36-37 -4.900092911376831 2315 38-39 -1.9873535443743364 2315 40-41 -1.5401121437946586 2315 42-43 -1.734649977044235 2315 44-45 -1.717018858717573 2315 46-47 -1.154522822807742 2315 48-49 -4.518451488704148 2315 50-51 -3.9838496242448116 2315 52-53 -2.4174467389330445 2315 54-55 -3.8430870593346107 2315 56-57 -6.406427789795895 2315 58-59 -6.802628920903274 2315 60-61 -5.942648876589342 2315 62-63 -6.904571349569338 2315 64-65 -6.112426905478575 2315 66-67 -4.646902041805276 2315 68-69 -2.1611193792464896 2315 70-71 0.3292169677992085 2315 72-73 -1.2502089188625263 2315 74-75 -2.6622511937173634 2315 76-77 -5.1974589050633355 2315 78-79 -4.29619633985957 2315 80-81 -3.694659084333157 2315 82-83 -7.4738096696718515 2315 84-85 -5.443376982231339 2315 86-87 -6.052568845143764 2315 88-89 -4.170040438289593 2315 90-91 -3.6981733008994944 2315 92-93 -4.837958594594198 2315 94-95 -3.0227499510142657 2315 96-97 -1.6541868323013666 2315 98-99 -3.06131786177696 2315 100-101 -2.3565690916353113 2316 1 -0.41367591271936277 2316 2 -0.42040386365225046 2316 3 -0.8889562471864529 2316 4 -0.33856891885795903 2316 5 -0.11725875335140046 2316 6 -2.2521748017300425 2316 7 -0.28306577078227235 2316 8 -0.46672061270370335 2316 9 0.008121195525781388 2316 10-11 -0.11273175729971285 2316 12-13 -0.5052650280213484 2316 14-15 -1.0045981006563096 2316 16-17 -1.2372795098298113 2316 18-19 -0.2243477513125498 2316 20-21 -4.125629228453754 2316 22-23 -4.173308822059134 2316 24-25 -2.662922179871174 2316 26-27 -1.9537011929964336 2316 28-29 -1.4290595856047972 2316 30-31 -0.4337600300898856 2316 32-33 -2.1047093688959535 2316 34-35 -2.6093858046541385 2316 36-37 -4.772002863871084 2316 38-39 -2.24100026284151 2316 40-41 -1.5478506013200857 2316 42-43 -2.4152911861090516 2316 44-45 -0.7157086959649277 2316 46-47 -0.948569980762386 2316 48-49 -0.7857009768909435 2316 50-51 -0.16495561502821943 2316 52-53 -2.900161285494214 2316 54-55 -1.2107768254419042 2316 56-57 -0.006098569411900456 2316 58-59 -0.09510111699689361 2316 60-61 -1.2347151791242972 2316 62-63 -0.6516198275672949 2316 64-65 0.2781561268269108 2316 66-67 1.2582212281080096 2316 68-69 3.466415775799316 2316 70-71 3.4351615101710173 2316 72-73 2.0099803136943244 2316 74-75 1.1094805134069183 2316 76-77 -0.2859440960743953 2316 78-79 0.09035502721522803 2316 80-81 -0.3898361835577333 2316 82-83 -0.18309858526022182 2316 84-85 0.3522672700471219 2316 86-87 -0.2941640423993377 2316 88-89 0.10213628942578268 2316 90-91 0.10861780817402789 2316 92-93 0.7148340479428157 2316 94-95 1.2205395653123787 2316 96-97 1.0130041629480573 2316 98-99 0.3035365777579422 2316 100-101 0.3275702322179477 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 27.0 4 65.0 5 153.0 6 408.0 7 1027.0 8 2127.0 9 3679.0 10 5907.0 11 9086.0 12 11745.0 13 15120.0 14 18379.0 15 23518.0 16 27835.0 17 30923.0 18 26599.0 19 20972.0 20 19323.0 21 19511.0 22 20489.0 23 23452.0 24 28155.0 25 34863.0 26 43724.0 27 54043.0 28 65813.0 29 78508.0 30 92637.0 31 107225.0 32 123352.0 33 143127.0 34 166350.0 35 190503.0 36 199673.0 37 171651.0 38 75142.0 39 4814.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.32943346607761 21.162269792655415 15.81325811456521 20.695038626701766 2 15.632992258600181 22.43602428893769 39.387140973493395 22.54384247896873 3 18.762611341376275 27.684706922238593 31.328494090916177 22.224187645468948 4 12.384962254016676 18.699114430466043 37.69689553333373 31.219027782183552 5 12.240172092220526 39.91095378166848 33.46211933440613 14.386754791704861 6 28.948139529757256 38.12067423934088 17.732412941756504 15.198773289145361 7 26.3766490513368 35.851183886731405 20.66359379612061 17.108573265811188 8 26.611818812828204 34.51307116976795 19.509519966579443 19.36559005082441 9 27.13248272921659 16.807057028398333 20.920807362415744 35.13965287996933 10-11 24.612848957462447 28.068161595068208 26.436651212704977 20.88233823476437 12-13 26.205997742929448 26.0014204812754 27.55459470851907 20.237987067276077 14-15 23.14457026140189 26.46189405747411 27.640216072299378 22.75331960882462 16-17 20.373590835102 30.05539209295011 28.11824148586836 21.45277558607953 18-19 20.03889439692716 27.4398787466907 32.426711428737335 20.094515427644808 20-21 21.82329197430552 26.163557010805498 32.27961229775812 19.733538717130862 22-23 21.087318736750195 26.525928622160045 32.03362701774741 20.353125623342347 24-25 20.71580554670667 26.83032851998661 32.42346160411736 20.030404329189366 26-27 20.507730013917225 26.85908656018749 32.61633328861828 20.016850137277004 28-29 20.043670517480304 27.023639941127346 32.83344276505735 20.099246776335 30-31 20.37392303026914 26.86335119859257 32.56341378435227 20.199311986786018 32-33 20.232904518019655 26.933580675081664 32.32380261053084 20.50971219636784 34-35 20.765917473604745 26.91521712654318 32.21031893153754 20.108546468314533 36-37 20.97328539052026 26.633260622578437 32.12701293347262 20.26644105342868 38-39 20.443894267318807 27.218643828335765 32.11644319458554 20.22101870975989 40-41 20.49295905429203 26.979252484402423 32.275632683897456 20.252155777408092 42-43 20.483431800114857 27.14911352985785 31.57699704034528 20.790457629682017 44-45 20.682182683199862 27.2956223628692 31.264181305433564 20.75801364849737 46-47 20.969784483964926 27.599469767261624 30.52532975395737 20.905415994816074 48-49 20.944851885529943 27.70298580924545 30.315626083991674 21.03653622123293 50-51 20.89487293543801 27.977399980571043 30.039587169677485 21.088139914313462 52-53 21.15197531754342 28.244522692126818 29.52629183109262 21.077210159237143 54-55 20.760750851406097 28.075768417541386 29.678079722815532 21.485401008236984 56-57 21.155815017317618 28.418727394918392 29.199613711779616 21.225843875984378 58-59 21.179316152359526 27.83617275041486 29.416923793350808 21.56758730387481 60-61 21.158436766627766 28.04419462907714 29.816426570224813 20.980942034070278 62-63 21.042780978680483 27.886995588991187 29.854367888027905 21.21585554430042 64-65 20.913929742504607 27.947448690317035 29.690699588167597 21.44792197901077 66-67 20.701723217605263 28.085182672190136 29.720839449946286 21.492254660258318 68-69 20.847606000904957 27.658010121518405 30.061023532923404 21.433360344653234 70-71 20.381360864936795 27.95468649894401 30.413025609937776 21.25092702618142 72-73 21.123528740928187 27.893618839665013 29.38069688686339 21.602155532543414 74-75 20.579949353046423 28.45451400061121 29.23295848484563 21.732578161496736 76-77 20.720458879086628 28.26295853762673 29.248406430846035 21.768176152440606 78-79 20.72169463464811 28.28215018425611 29.068185918139044 21.92796926295674 80-81 20.53528439705502 28.67762043132767 28.906341360341116 21.8807538112762 82-83 20.83548170821207 28.64659640965562 28.547936941814573 21.969984940317744 84-85 20.552514526060886 28.39844230948104 28.56917426595474 22.479868898503334 86-87 20.514180502981162 28.385119766571925 28.814705710777904 22.285994019669015 88-89 20.67388793141223 28.092042495296177 28.719218828086206 22.51485074520538 90-91 20.34971827309296 28.311947860829946 28.987694560126158 22.35063930595093 92-93 20.524979273101486 28.283049321953524 28.90695316204832 22.28501824289667 94-95 20.079363496887055 28.00760277187535 29.214895785225465 22.698137946012128 96-97 20.031765057143687 27.88423916382556 29.173084530406392 22.91091124862436 98-99 20.23083046970804 27.94176836670249 28.63065162139759 23.19674954219188 100-101 20.113684205998382 27.734445782102497 29.028455194937525 23.1234148169616 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3599.0 1 2441.0 2 1354.5 3 1486.0 4 1372.0 5 1381.5 6 2225.5 7 4067.5 8 5937.5 9 6979.0 10 6504.0 11 5369.5 12 4964.0 13 4795.5 14 4817.5 15 4934.0 16 5032.0 17 5181.5 18 5420.0 19 5764.5 20 6191.5 21 6867.0 22 7689.5 23 8530.0 24 9502.5 25 11398.0 26 14390.5 27 17522.0 28 20011.5 29 22599.0 30 25818.5 31 29572.0 32 34769.5 33 40423.5 34 45579.0 35 50085.0 36 55547.5 37 60577.0 38 60875.0 39 59603.0 40 58694.5 41 59106.0 42 60603.5 43 61418.5 44 62026.0 45 62033.5 46 61450.5 47 63204.0 48 69603.0 49 69210.0 50 61099.0 51 54915.5 52 51076.0 53 49389.0 54 47653.5 55 44169.5 56 39874.5 57 35537.5 58 32202.5 59 29265.0 60 25775.5 61 21917.0 62 17526.5 63 13182.0 64 9952.0 65 7662.0 66 5668.5 67 4312.0 68 3103.5 69 2062.0 70 1517.5 71 1180.5 72 1005.0 73 922.5 74 754.5 75 570.5 76 390.5 77 216.5 78 113.5 79 69.0 80 45.0 81 28.0 82 21.5 83 17.5 84 8.0 85 1.5 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025162196702784457 2 0.017258686199986776 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 5.645364644855487E-4 18-19 0.0013441344392513064 20-21 2.688268878502613E-5 22-23 0.0 24-25 2.688268878502613E-5 26-27 4.032403317753919E-4 28-29 0.017527513087837038 30-31 0.004570057093454442 32-33 0.008494929656068257 34-35 0.009489589141114222 36-37 0.10140150209711855 38-39 0.10460054206253666 40-41 0.11788059032233957 42-43 0.10462742475132168 44-45 0.09962724463730684 46-47 0.10113267520926829 48-49 0.10363276526627573 50-51 0.10115955789805331 52-53 0.07779850134386561 54-55 0.06683036431957495 56-57 0.037608881610251554 58-59 0.01500054034204458 60-61 0.008575577722423334 62-63 0.0022043804803721424 64-65 0.004005520628968893 66-67 0.4342898373220971 68-69 0.8411324493946826 70-71 0.9326948873964815 72-73 0.2879942449539849 74-75 0.15917240029613972 76-77 0.014570417321484161 78-79 0.00846804696728323 80-81 0.0013172517504662802 82-83 0.0 84-85 0.0 86-87 1.3441344392513063E-4 88-89 5.107710869154964E-4 90-91 0.00346786685326837 92-93 0.00166672670467162 94-95 0.23086853128580437 96-97 0.23844944952318173 98-99 0.013790819346718403 100-101 0.007097029839246897 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1859933.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.27228292511904 #Duplication Level Percentage of deduplicated Percentage of total 1 68.45858529014787 26.885249301680798 2 14.121763016535072 11.091877451736957 3 6.073460768136651 7.155560088626201 4 3.313109382372565 5.204534761056072 5 1.9853929292924728 3.898545641835243 6 1.3215671484390317 3.114057537482427 7 0.9042112304402417 2.485730748614343 8 0.6295833417895738 1.9780140098961654 9 0.48039345095974373 1.6979532769318824 >10 2.394718352686715 16.696299536488883 >50 0.17928949142281161 4.883613496197198 >100 0.1292968871095758 9.38614451615891 >500 0.006401946624463512 1.7225484927891603 >1k 0.0018092457851744707 1.5006985012854415 >5k 0.0 0.0 >10k+ 4.175182581171855E-4 2.2991726392203584 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17060 0.9172373413450915 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14766 0.7938995651993916 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10240 0.550557466317335 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4957 0.26651497661474904 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3450 0.1854905526166803 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3079 0.1655435975381909 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2822 0.15172589550268747 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1938 0.10419730173076126 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.6882688785026127E-5 0.0 12-13 0.0 0.0 0.0 5.3765377570052254E-5 0.0 14-15 0.0 0.0 0.0 5.3765377570052254E-5 0.0 16-17 0.0 0.0 0.0 5.3765377570052254E-5 0.0 18-19 0.0 0.0 0.0 5.3765377570052254E-5 0.0 20-21 0.0 0.0 0.0 8.064806635507838E-5 0.0 22-23 0.0 0.0 0.0 5.645364644855487E-4 0.0 24-25 0.0 0.0 0.0 0.001451665194391411 0.0 26-27 0.0 0.0 0.0 0.0029570957663528743 0.0 28-29 0.0 0.0 0.0 0.006398079930836218 0.0 30-31 0.0 0.0 0.0 0.01500054034204458 0.0 32-33 0.0 0.0 0.0 0.030162376816799313 0.0 34-35 0.0 0.0 0.0 0.05607728880556449 0.0 36-37 0.0 0.0 0.0 0.08908923063357659 0.0 38-39 0.0 0.0 0.0 0.1404620489017615 0.0 40-41 0.0 0.0 0.0 0.2022922331073216 0.0 42-43 0.0 0.0 0.0 0.260251310127838 0.0 44-45 0.0 0.0 0.0 0.3328883352249785 0.0 46-47 0.0 0.0 0.0 0.41643973196883977 0.0 48-49 0.0 0.0 0.0 0.5100990196958708 0.0 50-51 0.0 0.0 0.0 0.6190545573415817 0.0 52-53 0.0 0.0 0.0 0.7230905629396327 0.0 54-55 0.0 0.0 0.0 0.8335246484685201 0.0 56-57 0.0 0.0 0.0 0.95691618999179 0.0 58-59 0.0 0.0 0.0 1.0779420549019776 0.0 60-61 0.0 0.0 0.0 1.2084306262644944 0.0 62-63 0.0 0.0 0.0 1.3378976554531803 0.0 64-65 5.3765377570052254E-5 0.0 0.0 1.4709400822502747 0.0 66-67 5.3765377570052254E-5 0.0 0.0 1.604090039802509 0.0 68-69 5.3765377570052254E-5 0.0 0.0 1.7683432682790186 0.0 70-71 1.0753075514010451E-4 0.0 0.0 1.9850715052638992 0.0 72-73 1.0753075514010451E-4 0.0 0.0 2.2281178945693205 0.0 74-75 1.0753075514010451E-4 0.0 0.0 2.4775892464943627 0.0 76-77 1.0753075514010451E-4 0.0 0.0 2.7484054533147164 0.0 78-79 1.0753075514010451E-4 0.0 0.0 3.069357874719143 0.0 80-81 1.0753075514010451E-4 0.0 0.0 3.432758061715126 0.0 82-83 1.0753075514010451E-4 0.0 0.0 3.804142407280262 0.0 84-85 1.0753075514010451E-4 0.0 0.0 4.16082192207999 0.0 86-87 1.0753075514010451E-4 0.0 0.0 4.525915718469429 0.0 88-89 1.0753075514010451E-4 0.0 0.0 4.919585813037351 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 13280 0.0 32.940586 1 GTATCAA 19180 0.0 28.71344 1 GTACATG 14720 0.0 24.534367 1 TACATGG 14995 0.0 23.92124 2 ACATGGG 14955 0.0 22.873186 3 GAGTACT 13205 0.0 22.783628 12-13 TATCAAC 24340 0.0 22.52408 2 ATCAACG 24340 0.0 22.462658 3 TCAACGC 24475 0.0 22.280684 4 CAACGCA 24725 0.0 22.017075 5 AACGCAG 25240 0.0 21.586609 6 GTACTTT 14210 0.0 21.155588 14-15 AGTACTT 13800 0.0 20.736977 12-13 CATGGGA 10390 0.0 20.565388 4 CATGGGG 4225 0.0 19.960398 4 CATTCCG 290 2.5211193E-9 19.604652 9 ACGCAGA 27815 0.0 19.486008 7 CGCAGAG 27800 0.0 19.445395 8 GTCGGGA 635 0.0 19.401436 2 ACTTTTT 16130 0.0 19.048605 16-17 >>END_MODULE