FastQCFastQC Report
Fri 27 May 2016
SRR939440_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939440_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1846516
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120650.6533926594732999No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82160.44494604974990737No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62870.3404790426944581No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34540.1870549727161855No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20160.10917858280134046No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA106900.035.198051
GTATCAA163200.034.1530081
GTACATG128550.029.2701021
TACATGG132500.027.9367332
TATCAAC206750.027.1628072
ATCAACG213350.026.432883
TCAACGC215000.026.1629034
ACATGGG137600.025.9602893
CAACGCA214200.025.800035
AACGCAG217950.025.159996
CATTCCG4050.025.1187069
AACGCAT3900.024.8419886
GAGTACT110700.024.67950412-13
CATGGGA94900.023.3576494
CAACGCG3750.023.268915
CGCATAG3550.023.1994518
ACGCATA4000.023.0105297
ACGCAGA235850.022.5938287
CATGGGG38900.022.5481344
AGTACTT117450.022.54509712-13
Produced by FastQC (version 0.11.5)