FastQCFastQC Report
Fri 27 May 2016
SRR939440_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939440_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1846516
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168630.9132333540570458No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141390.7657122927719012No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102700.5561825621873843No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA49110.2659603274490987No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34220.18532197933838646No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29080.15748577320748913No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT27230.14746690524208836No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19400.1050627235290677No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA126200.033.799131
TATCGCG600.003992398731.6002337
GTATCAA185400.029.9676231
GTACATG150050.023.6521591
TATCAAC234400.023.6163352
ATCAACG238350.023.1813853
TCAACGC238900.023.1317794
CAACGCA241900.022.8477925
TACATGG153400.022.7279992
AACGCAG246300.022.4588766
GAGTACT126900.022.25908912-13
ACATGGG153050.021.8776153
AGTACTT134500.021.49464412-13
CATTCCG4650.021.406619
TTAGGCA6000.021.3457664
TATATCG1353.851377E-421.066825
GTACTTT139950.020.26814514-15
ACGCAGA276650.019.8750787
CGCAGAG278150.019.7167748
CATGGGG41250.019.3189964
Produced by FastQC (version 0.11.5)