##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939440_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1846516 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.87687244518867 31.0 31.0 31.0 28.0 34.0 2 31.100958778586268 31.0 31.0 34.0 28.0 34.0 3 31.08719610336439 31.0 31.0 34.0 28.0 34.0 4 35.02680507507111 35.0 35.0 37.0 32.0 37.0 5 34.616350467583274 35.0 35.0 37.0 31.0 37.0 6 34.69424689523405 35.0 35.0 37.0 31.0 37.0 7 34.51834915050831 35.0 35.0 37.0 30.0 37.0 8 34.43225403949925 35.0 35.0 37.0 30.0 37.0 9 35.888176977616226 37.0 35.0 39.0 30.0 39.0 10-11 35.68214653975379 37.0 35.0 39.0 30.0 39.0 12-13 35.67397710065875 37.0 35.0 39.0 30.0 39.0 14-15 36.409441618702466 38.0 35.0 40.0 30.0 41.0 16-17 36.35481793821445 38.0 34.5 40.0 30.0 41.0 18-19 36.44999393452318 38.0 35.0 40.0 30.0 41.0 20-21 36.369391329400884 38.0 34.5 40.0 30.0 41.0 22-23 36.20009385242261 38.0 34.0 40.0 29.5 41.0 24-25 36.034475737009586 38.0 34.0 40.0 29.0 41.0 26-27 35.56318141841176 38.0 34.0 40.0 27.0 41.0 28-29 35.35359319930073 38.0 34.0 40.0 27.0 41.0 30-31 34.9930891473456 37.0 33.0 40.0 27.0 41.0 32-33 34.66167338923681 37.0 33.0 40.0 25.5 41.0 34-35 34.81368425727153 37.0 33.0 40.0 26.0 41.0 36-37 34.59117603096859 37.0 33.0 40.0 25.0 41.0 38-39 34.369971340622016 37.0 33.0 40.0 25.0 41.0 40-41 34.17650862489142 37.0 33.0 40.0 24.0 41.0 42-43 33.933510188917936 37.0 33.0 40.0 24.0 41.0 44-45 33.44554772338826 37.0 32.0 40.0 22.5 41.0 46-47 32.90528053913424 37.0 31.0 40.0 19.0 41.0 48-49 32.42424869321468 36.0 31.0 39.0 15.5 40.5 50-51 32.14657522599317 36.0 30.5 39.0 14.0 40.0 52-53 32.02503985884769 36.0 30.5 39.0 13.0 40.0 54-55 31.683591422982524 36.0 30.0 39.0 9.5 40.0 56-57 31.203950845809082 35.0 29.5 39.0 8.0 40.0 58-59 30.771326920535756 35.0 28.0 38.5 7.5 40.0 60-61 30.409594067963667 35.0 28.0 38.0 6.5 40.0 62-63 30.251033568081727 34.0 28.0 38.0 2.0 40.0 64-65 29.81254237710369 34.0 28.0 38.0 2.0 40.0 66-67 28.95612223235542 33.5 27.0 37.5 2.0 39.5 68-69 25.532191705893695 31.0 15.5 36.0 2.0 39.0 70-71 24.238023932638548 29.0 10.5 35.0 2.0 38.0 72-73 25.254932261621345 30.0 16.5 35.0 2.0 38.0 74-75 25.997058243741186 31.0 21.0 35.0 2.0 37.0 76-77 25.653386702308563 30.5 21.0 34.0 2.0 36.0 78-79 26.750871370732774 32.0 24.5 35.0 2.0 37.0 80-81 26.75872616321765 32.0 25.0 35.0 2.0 36.5 82-83 26.424560090462254 32.0 24.0 35.0 2.0 36.0 84-85 26.064018129276974 32.0 23.5 34.5 2.0 36.0 86-87 25.627629275890378 31.5 21.5 34.0 2.0 35.0 88-89 25.144162032714583 31.0 20.0 34.0 2.0 35.0 90-91 24.554692729442905 31.0 18.0 34.0 2.0 35.0 92-93 24.000509337584944 31.0 13.0 34.0 2.0 35.0 94-95 23.167115259223316 30.0 4.5 34.0 2.0 35.0 96-97 22.52059716785557 30.0 2.0 34.0 2.0 35.0 98-99 21.69908492534048 29.5 2.0 34.0 2.0 35.0 100-101 20.33676285501994 27.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.171777268504421 1101 2 0.16537566733377318 1101 3 0.27916728248605693 1101 4 0.3084429945610907 1101 5 0.41952893163906424 1101 6 0.26611618337742016 1101 7 0.4068746638769909 1101 8 0.5899972134997284 1101 9 0.4846785011401096 1101 10-11 0.6074589662341978 1101 12-13 0.48939376669817136 1101 14-15 0.5507357749324981 1101 16-17 0.6200730554059959 1101 18-19 0.47692404229860585 1101 20-21 0.5897991770351751 1101 22-23 0.5670490535512798 1101 24-25 0.6028528363211549 1101 26-27 0.7141455726287589 1101 28-29 0.5064168476178352 1101 30-31 0.23680732819633477 1101 32-33 0.7235961719635142 1101 34-35 0.6910999930795612 1101 36-37 0.4916211633593619 1101 38-39 0.6549049664107898 1101 40-41 0.5683062440992046 1101 42-43 0.2739941523343603 1101 44-45 0.42169777285391064 1101 46-47 0.30919374810293476 1101 48-49 0.33020981433990215 1101 50-51 -0.08985169455198161 1101 52-53 -0.46852432218076956 1101 54-55 -0.20795960608423414 1101 56-57 0.008215081255187329 1101 58-59 -0.04760132439145082 1101 60-61 -0.22128391116341461 1101 62-63 -0.5824369827641398 1101 64-65 -0.6351079500975985 1101 66-67 -0.7373602420839838 1101 68-69 -7.392950551855208 1101 70-71 -7.8561822283045935 1101 72-73 -5.235214373743755 1101 74-75 -3.3340460867842587 1101 76-77 -1.0548252463910863 1101 78-79 -0.5750334587250414 1101 80-81 -0.6476975952685571 1101 82-83 -0.6376721483843291 1101 84-85 -0.8005631225544718 1101 86-87 -1.092297737388904 1101 88-89 -1.0151178235875982 1101 90-91 -1.2034959182155234 1101 92-93 -1.1131794377681494 1101 94-95 -1.1822313446731343 1101 96-97 -1.4115516257375518 1101 98-99 -1.3626233549061446 1101 100-101 -1.176440357158775 1102 1 0.3321307696119362 1102 2 0.3293494227502727 1102 3 0.3860099893462028 1102 4 0.3387863568900329 1102 5 0.43697667172470034 1102 6 0.5209811826347703 1102 7 0.545130793963331 1102 8 0.5896013490881344 1102 9 0.518472922874551 1102 10-11 0.5908707620895584 1102 12-13 0.6145101689170787 1102 14-15 0.6290759212667112 1102 16-17 0.6028810450218245 1102 18-19 0.4622412181644435 1102 20-21 0.5621012609739324 1102 22-23 0.6293482960489101 1102 24-25 0.681550100142502 1102 26-27 0.5862994495814462 1102 28-29 0.6512628608821913 1102 30-31 0.652121081417171 1102 32-33 0.9195655231462609 1102 34-35 0.7748479185046406 1102 36-37 0.9177288410624485 1102 38-39 0.9732083677449808 1102 40-41 0.903136823171991 1102 42-43 0.801234854686939 1102 44-45 0.7320790425906267 1102 46-47 0.6977724463354278 1102 48-49 0.6485287144438701 1102 50-51 0.5397373943140948 1102 52-53 0.4338179294030091 1102 54-55 0.36715195276911317 1102 56-57 0.4188814637777405 1102 58-59 0.5224395536138573 1102 60-61 0.5768617256099979 1102 62-63 0.23164946162433608 1102 64-65 0.12404181322326835 1102 66-67 0.09252106317074293 1102 68-69 -6.743934723736565 1102 70-71 -7.471939310919495 1102 72-73 -1.5880871353016417 1102 74-75 0.010277165890894224 1102 76-77 0.5194994270046323 1102 78-79 0.4351043511696311 1102 80-81 0.28131577887820214 1102 82-83 0.04584200392982751 1102 84-85 -0.27755616748153145 1102 86-87 -0.30726502852683524 1102 88-89 -0.3801403290928924 1102 90-91 -0.29132131165825115 1102 92-93 -0.3039250577512327 1102 94-95 -0.09648375505472728 1102 96-97 -0.29924269650880575 1102 98-99 -0.3709310184020218 1102 100-101 -0.3768426793911175 1103 1 0.34164602061190763 1103 2 0.2899768656840962 1103 3 0.32797930749059745 1103 4 0.3926024633443248 1103 5 0.49497442226236643 1103 6 0.5078301874756761 1103 7 0.441121026027794 1103 8 0.49404099738165996 1103 9 0.6178406415701687 1103 10-11 0.5430969191820836 1103 12-13 0.4450541562917181 1103 14-15 0.5547179553075594 1103 16-17 0.6516665760139873 1103 18-19 0.6398309496728771 1103 20-21 0.6213849161475622 1103 22-23 0.633150913328727 1103 24-25 0.5488213731405907 1103 26-27 0.8175700791522473 1103 28-29 0.6693522871426367 1103 30-31 0.5681823184694679 1103 32-33 0.8856108011752397 1103 34-35 0.9074541030061454 1103 36-37 1.0545144531163189 1103 38-39 1.247123470807331 1103 40-41 1.00098463488591 1103 42-43 0.7635474944867156 1103 44-45 0.8085107316379236 1103 46-47 0.8320071478383682 1103 48-49 1.0158762994383395 1103 50-51 0.8278135973838019 1103 52-53 0.7171304906742613 1103 54-55 0.7155171661513862 1103 56-57 0.8701013157894621 1103 58-59 1.0419915736968939 1103 60-61 0.9740789659148952 1103 62-63 0.5786185771144723 1103 64-65 0.6743893652864195 1103 66-67 0.511491338856537 1103 68-69 -6.495037289592869 1103 70-71 -7.618397231028119 1103 72-73 -0.7096515122284401 1103 74-75 0.9258273342205889 1103 76-77 1.1913812745022305 1103 78-79 1.1316897534763442 1103 80-81 0.8720725912279548 1103 82-83 0.6948194865078925 1103 84-85 0.3718313733343024 1103 86-87 0.24240838146047494 1103 88-89 0.39751089531884176 1103 90-91 0.3684718923310015 1103 92-93 0.49173263133153 1103 94-95 0.8215796427986568 1103 96-97 0.5952526437097063 1103 98-99 0.5997407931497811 1103 100-101 0.7561621640457972 1104 1 0.19207675096859234 1104 2 0.2918407742500513 1104 3 0.231227360798254 1104 4 0.3092111813674947 1104 5 0.4083743137519349 1104 6 0.364187599425577 1104 7 0.4677315525580852 1104 8 0.47999195372531744 1104 9 0.4270800751763062 1104 10-11 0.44209880155779757 1104 12-13 0.41353678061384613 1104 14-15 0.6700284326770216 1104 16-17 0.7013670897828419 1104 18-19 0.5248506522116614 1104 20-21 0.5486628709552619 1104 22-23 0.5998473184750779 1104 24-25 0.49119399438061606 1104 26-27 0.7554679188777627 1104 28-29 0.6411446705257688 1104 30-31 0.4870281088507511 1104 32-33 0.6706799192605715 1104 34-35 0.27071122432131745 1104 36-37 0.6788158854030186 1104 38-39 0.621936570512112 1104 40-41 0.7451687561934364 1104 42-43 0.6714626423894856 1104 44-45 0.610216204791783 1104 46-47 0.37991953203660245 1104 48-49 0.38904136519412447 1104 50-51 0.22320228631802763 1104 52-53 0.17032008589980663 1104 54-55 0.2715006501511965 1104 56-57 0.34491467021355504 1104 58-59 0.45902599137033917 1104 60-61 0.6510162336427214 1104 62-63 0.23410338438110045 1104 64-65 0.2913876764973793 1104 66-67 0.20739946414787624 1104 68-69 -6.317175709161422 1104 70-71 -4.089877901653125 1104 72-73 0.33358931978672146 1104 74-75 0.8933722730752187 1104 76-77 0.8763934628213157 1104 78-79 0.4157304423392496 1104 80-81 0.3618437004541448 1104 82-83 0.3540203301382583 1104 84-85 0.27698186150386306 1104 86-87 0.11913155760652216 1104 88-89 0.11866616202269498 1104 90-91 0.17568089462092473 1104 92-93 0.3765227680903287 1104 94-95 0.3255440304916384 1104 96-97 0.26420828179431766 1104 98-99 0.35908230552022147 1104 100-101 0.5488598071280038 1105 1 0.221763499673024 1105 2 0.22134092957963958 1105 3 0.1475432991967196 1105 4 0.1508798762230228 1105 5 0.2980784356253139 1105 6 0.15112091851212028 1105 7 0.19503414052706347 1105 8 0.39303508786082375 1105 9 0.4290033918290206 1105 10-11 0.40784516516358593 1105 12-13 0.22186402938060468 1105 14-15 0.33760333220747185 1105 16-17 0.37580122509172753 1105 18-19 0.5421644595277826 1105 20-21 0.457888063237462 1105 22-23 0.40549834731097434 1105 24-25 0.41942480029383233 1105 26-27 0.5102311946105331 1105 28-29 0.3677032594603702 1105 30-31 0.30967634966899027 1105 32-33 0.5586546391231693 1105 34-35 0.48237894960362837 1105 36-37 0.6030338454605584 1105 38-39 0.709565212100479 1105 40-41 0.6456598747049611 1105 42-43 0.5349823514383729 1105 44-45 0.7462690920422901 1105 46-47 0.5392312933553782 1105 48-49 0.7065657084072114 1105 50-51 0.43389450222812087 1105 52-53 0.42156816406718534 1105 54-55 0.3593914722503442 1105 56-57 0.5621633776141692 1105 58-59 0.5609192314663467 1105 60-61 0.8238278178659861 1105 62-63 0.20625981413552452 1105 64-65 0.23387120859322152 1105 66-67 0.3031547122102154 1105 68-69 -3.1747968513978115 1105 70-71 -3.4481727983342125 1105 72-73 0.4838316141531074 1105 74-75 1.0482042104210052 1105 76-77 0.9490283472739129 1105 78-79 0.6391319821798085 1105 80-81 0.5005124566500143 1105 82-83 0.44048746259580795 1105 84-85 0.6380618077618081 1105 86-87 0.48886247136001515 1105 88-89 0.5506555481641335 1105 90-91 0.30500415230775957 1105 92-93 0.23100071227852936 1105 94-95 0.2681551900814121 1105 96-97 0.23694051931204996 1105 98-99 0.11323316715420972 1105 100-101 0.36514151365600256 1106 1 0.07450081700261535 1106 2 0.08764124276836327 1106 3 0.04668953310689972 1106 4 0.11049675858668451 1106 5 0.15302404647221124 1106 6 0.1905719268472481 1106 7 0.2295417474595638 1106 8 0.2769821864001969 1106 9 0.42109565269810645 1106 10-11 0.2957526925560856 1106 12-13 0.06263478941102818 1106 14-15 0.02670034614354222 1106 16-17 0.08933411727269203 1106 18-19 0.17341741696099433 1106 20-21 0.20497378323090487 1106 22-23 0.17247689084531004 1106 24-25 0.17078429897092207 1106 26-27 0.47209733727066805 1106 28-29 0.3428188179986762 1106 30-31 0.2010092061306068 1106 32-33 0.4009730458192635 1106 34-35 0.2578353149205128 1106 36-37 0.2696014323607585 1106 38-39 0.30503230703109097 1106 40-41 0.22348075966104375 1106 42-43 0.3033312107207067 1106 44-45 0.4034941819390525 1106 46-47 0.3363823300450477 1106 48-49 0.3664237193918325 1106 50-51 0.33365443229921965 1106 52-53 0.17881376036858754 1106 54-55 -2.4575541263516243E-4 1106 56-57 0.3892481263727383 1106 58-59 0.48419961638836284 1106 60-61 0.507330795092134 1106 62-63 0.2134568486930739 1106 64-65 0.2645492272004226 1106 66-67 0.36718187548630254 1106 68-69 -3.2681748707415004 1106 70-71 -3.3904624592397568 1106 72-73 0.37785435393764644 1106 74-75 0.7315701330573141 1106 76-77 0.7616263913633716 1106 78-79 0.3795863774088808 1106 80-81 0.20819768633095137 1106 82-83 0.12301494716191641 1106 84-85 0.2687330599459372 1106 86-87 0.3066389004561856 1106 88-89 0.3211076372857491 1106 90-91 0.42085808870890773 1106 92-93 0.21598949474585183 1106 94-95 0.487743685689221 1106 96-97 0.46904252475096797 1106 98-99 0.48426042525228397 1106 100-101 0.6048781847231481 1107 1 0.006028536857861866 1107 2 -0.013826140493446104 1107 3 0.0851784949408092 1107 4 -0.12303343661598376 1107 5 0.25153513845020115 1107 6 0.09760850548661182 1107 7 0.12389385217752391 1107 8 0.03726362263981997 1107 9 0.17801552677826038 1107 10-11 0.04316635808822156 1107 12-13 0.21809652914976851 1107 14-15 0.01749767034679195 1107 16-17 -0.06204380370453322 1107 18-19 0.01083821256994355 1107 20-21 0.10559581516827166 1107 22-23 0.06174124853301777 1107 24-25 0.08582371618803819 1107 26-27 0.2717707662994968 1107 28-29 0.16317703709382414 1107 30-31 0.12752737437351414 1107 32-33 0.24770330021501508 1107 34-35 0.21630850314108585 1107 36-37 0.12468178025469712 1107 38-39 0.2886112692685927 1107 40-41 0.18060360550340704 1107 42-43 5.106263234679886E-4 1107 44-45 0.10850491068671175 1107 46-47 0.2647729842632245 1107 48-49 0.17970399194911124 1107 50-51 0.30972579360577157 1107 52-53 0.051346821054952585 1107 54-55 0.06739916676190205 1107 56-57 0.3173117051331822 1107 58-59 0.42016765884595486 1107 60-61 0.19270186177853432 1107 62-63 0.030118022061561334 1107 64-65 -0.15215751812130307 1107 66-67 0.0408143805648038 1107 68-69 -2.792460854086716 1107 70-71 -2.9380548811788643 1107 72-73 0.8121945864050559 1107 74-75 0.9844154372963452 1107 76-77 0.5376570574201693 1107 78-79 0.39048076482765737 1107 80-81 0.13092547883226047 1107 82-83 0.2003745989989234 1107 84-85 0.17550478313751938 1107 86-87 0.13685815031829307 1107 88-89 0.06530267851911375 1107 90-91 0.32148684713383346 1107 92-93 0.45239274094874915 1107 94-95 0.5091683893874439 1107 96-97 0.4012521422828854 1107 98-99 0.775186898016738 1107 100-101 0.8602560115295432 1108 1 -0.009054727692166864 1108 2 0.020851985156319586 1108 3 0.055670521328394784 1108 4 -0.006977460659690848 1108 5 -0.022092272378287703 1108 6 -0.1489583991387704 1108 7 0.10415760201212976 1108 8 0.25149956091757275 1108 9 0.08642212745495925 1108 10-11 0.1950313201891447 1108 12-13 0.140049260844485 1108 14-15 0.1457491462999414 1108 16-17 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0.24967905648873057 2311 6 0.2502379926901668 2311 7 -3.306958922818737 2311 8 -0.8985654585882799 2311 9 -3.072586283929212 2311 10-11 -1.3307993657254897 2311 12-13 -1.0316456906789497 2311 14-15 -2.2633917731910955 2311 16-17 0.039716577570622746 2311 18-19 0.3285617401807386 2311 20-21 -0.8525920145006864 2311 22-23 0.06362225086258633 2311 24-25 0.42841404928597626 2311 26-27 0.5410123650608583 2311 28-29 0.42973565077051745 2311 30-31 0.4209376846741719 2311 32-33 0.6290346110069649 2311 34-35 0.36163211515749083 2311 36-37 0.5839070416111056 2311 38-39 0.5072523842511671 2311 40-41 0.5121068021069846 2311 42-43 0.5893784389818535 2311 44-45 0.53013504581984 2311 46-47 0.4774391982638022 2311 48-49 0.8036846032554408 2311 50-51 0.713742083111832 2311 52-53 0.9840050281736303 2311 54-55 1.108660661686237 2311 56-57 1.0395172418564158 2311 58-59 1.1604812412233017 2311 60-61 1.6263494398747618 2311 62-63 1.3525264170539266 2311 64-65 1.425854399640187 2311 66-67 1.5082999348423343 2311 68-69 3.9472342500642412 2311 70-71 5.248365168778378 2311 72-73 3.9927666027088016 2311 74-75 2.852475033871187 2311 76-77 2.4426319719950875 2311 78-79 1.5969947224992787 2311 80-81 1.4567673827224965 2311 82-83 1.3799957576212698 2311 84-85 1.4587566452068401 2311 86-87 1.5851411045923314 2311 88-89 1.772192383559048 2311 90-91 1.7946163793235073 2311 92-93 1.9528547386819675 2311 94-95 2.2437972150684935 2311 96-97 2.120755437273374 2311 98-99 2.3008555244384112 2311 100-101 2.5453891024772375 2312 1 -0.08571252855858447 2312 2 -0.04427695441687618 2312 3 -0.08391441689269286 2312 4 0.14713695264079263 2312 5 -1.244985962834356 2312 6 0.042377075033279255 2312 7 0.26870495694954855 2312 8 0.43154172535889046 2312 9 -2.5447247631398824 2312 10-11 0.040022913987236564 2312 12-13 -1.008806161378132 2312 14-15 -4.008010872202597 2312 16-17 -0.7484246961537266 2312 18-19 0.12781253087825206 2312 20-21 0.5205760849101608 2312 22-23 0.4282112480817801 2312 24-25 0.6489983201399667 2312 26-27 0.6526626907980742 2312 28-29 0.3618896469342232 2312 30-31 0.4126219960111399 2312 32-33 0.8977618735989452 2312 34-35 0.47599882151514805 2312 36-37 0.7631687435229466 2312 38-39 0.7375756449679614 2312 40-41 0.5205170729043118 2312 42-43 0.25926051744938405 2312 44-45 1.0289322078075074 2312 46-47 1.0592381230431371 2312 48-49 1.040794474797373 2312 50-51 1.0120374571773212 2312 52-53 0.834770640851108 2312 54-55 0.9974811904871856 2312 56-57 0.9056492529284661 2312 58-59 0.988745215847878 2312 60-61 1.067525520738375 2312 62-63 1.1175419020226762 2312 64-65 1.3995553117409436 2312 66-67 1.6318047832047853 2312 68-69 4.1565308057210295 2312 70-71 5.150860355141738 2312 72-73 3.2795108480484636 2312 74-75 2.684230779850072 2312 76-77 2.56448643065173 2312 78-79 1.9761747273791705 2312 80-81 1.892648463279972 2312 82-83 1.8659756696553167 2312 84-85 1.822589213810872 2312 86-87 2.076400938621248 2312 88-89 1.9132392478643006 2312 90-91 2.259642693261256 2312 92-93 2.4143714614430003 2312 94-95 2.717332494204591 2312 96-97 2.714395177122242 2312 98-99 2.7853330883681657 2312 100-101 2.6037915142759687 2313 1 -0.35615207692042006 2313 2 -0.24178610265424183 2313 3 -0.11259974940513118 2313 4 0.012416281359286074 2313 5 -0.10551453611850548 2313 6 -0.06782712894516862 2313 7 -2.7208804926546186 2313 8 -3.5346886584460044 2313 9 -1.010904475392067 2313 10-11 -0.21195101656942228 2313 12-13 -0.011507692610599918 2313 14-15 -0.5358133778038265 2313 16-17 -0.30482451326034976 2313 18-19 0.1492815681805979 2313 20-21 -2.78771249648144 2313 22-23 -0.8163346610586544 2313 24-25 -0.25589093753843883 2313 26-27 -2.022017376630778 2313 28-29 -1.8101585923839494 2313 30-31 -0.9172203131652594 2313 32-33 -1.2659650500287896 2313 34-35 -1.6042218590442445 2313 36-37 -2.456359930152736 2313 38-39 -2.233945990833469 2313 40-41 -1.181914352783835 2313 42-43 -0.3159539088606209 2313 44-45 -0.13971223537873811 2313 46-47 -0.27580979606889144 2313 48-49 -2.1795549352953536 2313 50-51 -0.5930493957671708 2313 52-53 -0.7439570131130928 2313 54-55 -0.42799832179079544 2313 56-57 -0.34220624607689487 2313 58-59 -0.2600580552982201 2313 60-61 0.050578384252048636 2313 62-63 0.294901614379544 2313 64-65 0.06779699934630301 2313 66-67 0.8693525464958398 2313 68-69 1.6898710221385151 2313 70-71 4.060480147212331 2313 72-73 3.1260759590905316 2313 74-75 1.9175799411050356 2313 76-77 1.6938746941685459 2313 78-79 1.227279153122982 2313 80-81 1.2245680682226592 2313 82-83 1.0201653982223213 2313 84-85 0.6799173908577529 2313 86-87 0.9734477624664706 2313 88-89 0.6744489610062168 2313 90-91 0.9626372154998819 2313 92-93 1.1755095683062144 2313 94-95 1.3279294621901592 2313 96-97 1.5537318603289307 2313 98-99 1.7662177803731502 2313 100-101 1.845651117439207 2314 1 -0.17188567938388033 2314 2 -0.10336707323995142 2314 3 -0.2512017647843976 2314 4 -0.08334543525550941 2314 5 -0.22162945318128635 2314 6 -2.346825360550035 2314 7 -0.6645405216227047 2314 8 -0.048431139013125346 2314 9 -0.019442412839957512 2314 10-11 -0.08218018537679228 2314 12-13 -2.1854161461399073 2314 14-15 -0.5715139395707425 2314 16-17 -1.7575498546112769 2314 18-19 -0.22144447790089572 2314 20-21 -0.3958640051068798 2314 22-23 -0.2932010123325952 2314 24-25 -0.20307719989057915 2314 26-27 -1.920821680648352 2314 28-29 -0.7061181684383158 2314 30-31 -0.26737263725123483 2314 32-33 0.2532648981111052 2314 34-35 0.1963253159105136 2314 36-37 -0.2461210208616862 2314 38-39 -0.09236646536768234 2314 40-41 -0.13838316470287992 2314 42-43 -0.5738653539758545 2314 44-45 -3.801330835385965 2314 46-47 -3.8997762186135105 2314 48-49 -3.4075528670847532 2314 50-51 -4.009795473650691 2314 52-53 -0.9836454020905805 2314 54-55 -1.7148037199706039 2314 56-57 -0.9317349446103336 2314 58-59 -1.7348071651184185 2314 60-61 -1.0101241598628157 2314 62-63 -1.2135429533067317 2314 64-65 -1.348515073363874 2314 66-67 -0.8695149862105573 2314 68-69 -1.5324803850470126 2314 70-71 1.423596792319497 2314 72-73 1.317594231177523 2314 74-75 0.803864955352136 2314 76-77 1.0086502008871818 2314 78-79 -1.4691600338375288 2314 80-81 -0.9351420820088165 2314 82-83 0.15151460066157085 2314 84-85 0.1611261398390127 2314 86-87 0.10058875690604197 2314 88-89 -0.174462108283997 2314 90-91 0.30660578695660945 2314 92-93 -0.13946417320781634 2314 94-95 0.36800925345478674 2314 96-97 0.5241793923878362 2314 98-99 0.4915307450226045 2314 100-101 0.4898895697345296 2315 1 -0.2877391730607819 2315 2 -0.4422970630939673 2315 3 -0.5157088876077438 2315 4 -0.2381563826742621 2315 5 -0.2942422650138141 2315 6 -0.19915360096862855 2315 7 -0.87837179089545 2315 8 -2.858425148185784 2315 9 -0.7257879939288685 2315 10-11 -1.5221908009520817 2315 12-13 -0.7603541389423825 2315 14-15 -0.6840248077284272 2315 16-17 -0.4666098069977167 2315 18-19 -0.14146052933378428 2315 20-21 -0.8436975597166168 2315 22-23 -0.29895917928172366 2315 24-25 -5.426214173540899 2315 26-27 -1.2510827583426973 2315 28-29 -1.1781562308254507 2315 30-31 -1.255955201809158 2315 32-33 -0.24879347047703249 2315 34-35 -2.335931945299521 2315 36-37 -3.5354758471775014 2315 38-39 -1.2899755418268626 2315 40-41 -0.45789277066012346 2315 42-43 -1.7710140901389693 2315 44-45 -5.4480918753708245 2315 46-47 -3.8127534036646153 2315 48-49 -2.8817133330316693 2315 50-51 -1.4974829716051268 2315 52-53 -0.5357983289868358 2315 54-55 -1.4504801640659366 2315 56-57 -1.4719730977848329 2315 58-59 -1.2490464083104342 2315 60-61 -2.250333568450049 2315 62-63 -0.9902167207402499 2315 64-65 -0.6036168818537782 2315 66-67 -0.26355347265150897 2315 68-69 2.5585417696301747 2315 70-71 3.2405670559016713 2315 72-73 -2.751691608128848 2315 74-75 -2.2008814590452026 2315 76-77 -0.9622323860118343 2315 78-79 -0.8652519083688297 2315 80-81 -1.181789139744172 2315 82-83 -1.6253918271688796 2315 84-85 -1.2083712994736189 2315 86-87 -0.8450804156625153 2315 88-89 -0.7561315996044122 2315 90-91 -0.7628017187958704 2315 92-93 -1.848086553288102 2315 94-95 -0.7756788193913984 2315 96-97 -0.8919803307534089 2315 98-99 -2.712180864233151 2315 100-101 -3.8194014777495333 2316 1 -0.5437472786236697 2316 2 -0.43736607313222464 2316 3 -0.9779038371941269 2316 4 -0.3881998166760141 2316 5 -0.16074186561156978 2316 6 -0.4668849168044886 2316 7 -0.7302308773362611 2316 8 -1.3461802534675797 2316 9 -5.4119991673456695 2316 10-11 -1.5721549897367382 2316 12-13 -0.7725235998366955 2316 14-15 -0.5313078401999078 2316 16-17 -0.5196239043883466 2316 18-19 -0.488888077131044 2316 20-21 -3.4274132234310386 2316 22-23 -1.0416369371026164 2316 24-25 -0.45866421649847666 2316 26-27 -0.38465620944807455 2316 28-29 -0.06531120048517636 2316 30-31 -0.1563960529567936 2316 32-33 -1.553855000656121 2316 34-35 -0.7541267424301736 2316 36-37 -0.4117087931780006 2316 38-39 -5.231533385277061 2316 40-41 -5.035627723200339 2316 42-43 -3.4990116317903457 2316 44-45 -2.3183591969618895 2316 46-47 -2.693797439548881 2316 48-49 -3.0740362342641774 2316 50-51 -1.3730886258069503 2316 52-53 -1.083958458320108 2316 54-55 -0.19402977808297095 2316 56-57 -3.696442718598849 2316 58-59 -1.1467462325099227 2316 60-61 -0.8610783623251415 2316 62-63 -0.5874771230645628 2316 64-65 -0.052667289117021454 2316 66-67 -0.16256584538670893 2316 68-69 2.407946477653635 2316 70-71 3.37310984276062 2316 72-73 2.207041325961047 2316 74-75 0.9635356915889979 2316 76-77 -1.6350758917948305 2316 78-79 -0.09825110956434102 2316 80-81 -0.13073790985522749 2316 82-83 0.19199082559255132 2316 84-85 0.3965976456121467 2316 86-87 0.34912356692576907 2316 88-89 0.06721826564695732 2316 90-91 0.2219471500937935 2316 92-93 -0.006973213570685033 2316 94-95 0.06862973829801078 2316 96-97 0.29106103397945304 2316 98-99 0.007660295954938334 2316 100-101 0.2378543826645405 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 27.0 4 77.0 5 195.0 6 489.0 7 1059.0 8 2277.0 9 3965.0 10 6543.0 11 9516.0 12 12731.0 13 15654.0 14 19285.0 15 24806.0 16 29212.0 17 32182.0 18 27509.0 19 22462.0 20 21604.0 21 21761.0 22 22744.0 23 25116.0 24 29848.0 25 36660.0 26 45303.0 27 56456.0 28 68628.0 29 81192.0 30 95051.0 31 109841.0 32 126537.0 33 145873.0 34 166678.0 35 184422.0 36 184525.0 37 149123.0 38 63287.0 39 3873.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.08024648224915 21.32631543596169 15.961491481943105 20.631946599846057 2 15.64572311922163 22.52308368951212 39.271385758012194 22.559807433254054 3 18.758966243868834 27.801001782282132 31.40103839190602 22.038993581943007 4 12.324036032910927 18.779336361369772 37.702739325087535 31.19388828063177 5 12.171949769186945 39.989959469617375 33.49627081487515 14.34181994632053 6 28.93080807314965 38.19035415885917 17.729388751573232 15.149449016417945 7 26.300611530038193 35.855416362490224 20.660205489689773 17.183766617781814 8 26.668222750303816 34.47032140528433 19.558292481624854 19.303163362787 9 27.063236928355888 16.818375795281494 21.02635449679288 35.09203277956974 10-11 24.602832229672035 28.132029900000244 26.4606011467462 20.804536723581524 12-13 26.132700718542377 26.079871498541035 27.605447231434766 20.181980551481818 14-15 23.102128549116284 26.50681066397475 27.6462267318561 22.744834055052866 16-17 20.3584123407227 30.06702664412678 28.100121066588247 21.47443994856227 18-19 20.04820433308268 27.509098899575392 32.42048735604296 20.022209411298963 20-21 21.83262966581389 26.182903370455495 32.226067903013025 19.758399060717586 22-23 21.094261944399015 26.557069158425012 32.07560300111453 20.273065896061443 24-25 20.67000045763579 26.877680994277387 32.45398661699648 19.99833193109034 26-27 20.50831288183243 26.901637555265083 32.61774066494931 19.97230889795317 28-29 20.092337228556303 27.049392293767777 32.76271092354829 20.09555955412762 30-31 20.33825928608661 26.911055300194285 32.57132025913716 20.179365154581948 32-33 20.29077651605412 26.978468500474413 32.293539575327216 20.437215408144255 34-35 20.70932563860104 26.94932676508212 32.245764593616535 20.095583002700305 36-37 20.92744803004154 26.703089361443798 32.12231802926102 20.247144579253646 38-39 20.454076054107063 27.305331506852287 32.078079354349434 20.162513084691223 40-41 20.464059896739478 27.04729756829384 32.27221927739314 20.216423257573542 42-43 20.4467478745004 27.215717477389973 31.584604260863813 20.75293038724581 44-45 20.676597489480333 27.272030055341567 31.27840621919879 20.77296623597931 46-47 20.95330338073735 27.6200639936608 30.558422873601003 20.86820975200085 48-49 20.968846860703774 27.65467768605791 30.331535609510755 21.04493984372756 50-51 20.856685435063135 27.947865806236223 30.128912815026425 21.066535943674218 52-53 21.110599937358636 28.187665070287295 29.570055911209657 21.13167908114441 54-55 20.746013513147414 28.062347950000273 29.702401915897724 21.48923662095459 56-57 21.137803115499707 28.383728892153613 29.22327567340341 21.25519231894327 58-59 21.191939261702068 27.76766929348827 29.478263177623553 21.56212826718611 60-61 21.12409052477526 28.073068917274245 29.833143489774177 20.969697068176316 62-63 21.05150425526211 27.91169786163229 29.844512134855616 21.19228574824998 64-65 20.910297936318248 27.959344329417846 29.708546621789772 21.421811112474135 66-67 20.85648781628262 28.160100417384164 29.40362803365653 21.579783732676685 68-69 21.182172070603055 27.674261705133834 29.702384963389783 21.441181260873325 70-71 20.636087014145744 27.9248402404216 30.23554375643035 21.20352898900231 72-73 21.1588418865141 27.911034019026477 29.384224256066517 21.54589983839291 74-75 20.870940150330636 28.402319089682248 28.984638293156927 21.742102466830186 76-77 20.132039439802423 28.38929357732944 29.70971510934728 21.76895187352086 78-79 20.77794827435855 28.266298928471418 29.075971121863287 21.879781675306745 80-81 20.524379072171172 28.664077374400232 28.912851982763687 21.898691570664905 82-83 20.837212350248286 28.61276606806598 28.586905955299645 21.963115626386088 84-85 20.543429844097997 28.3686408838283 28.603813946561424 22.48411532551228 86-87 20.527671433778274 28.330772592691684 28.80421625901517 22.33733971451488 88-89 20.608003180064376 28.15645895065999 28.711101820278163 22.52443604899747 90-91 20.37796608654892 28.290400612423234 28.909187458754992 22.422445842272857 92-93 20.490210042823108 28.290655550458645 28.867470414577728 22.351663992140523 94-95 20.277171332620775 27.957946792296568 29.00461572816418 22.760266146918475 96-97 20.35048331503949 27.87358719337044 28.84325119704582 22.932678294544246 98-99 20.233803402785753 27.866483015319687 28.65326618468118 23.246447397213384 100-101 20.107010698361663 27.734765992295795 29.001702898393727 23.156520410948815 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3471.0 1 2356.0 2 1337.5 3 1460.0 4 1354.5 5 1361.0 6 2128.5 7 4079.5 8 6069.0 9 6934.0 10 6299.5 11 5163.5 12 4853.5 13 4701.0 14 4646.0 15 4806.0 16 4959.0 17 5099.0 18 5323.0 19 5678.5 20 6208.0 21 6888.0 22 7698.5 23 8569.5 24 9626.0 25 11399.5 26 14306.5 27 17509.5 28 19986.0 29 22424.5 30 25447.0 31 29473.5 32 34637.0 33 39877.5 34 45144.0 35 49857.0 36 55369.0 37 60669.5 38 61267.0 39 59535.0 40 58372.0 41 59026.5 42 60435.0 43 61029.5 44 61114.5 45 61053.0 46 60609.0 47 62199.5 48 68819.0 49 68661.0 50 60794.0 51 54379.5 52 50480.5 53 48974.0 54 46932.5 55 43488.0 56 39145.5 57 35091.0 58 32053.5 59 29169.0 60 25927.5 61 21927.5 62 17497.0 63 13249.5 64 9862.5 65 7574.0 66 5662.0 67 4394.5 68 3257.0 69 2155.5 70 1543.5 71 1207.5 72 1004.5 73 902.5 74 739.0 75 574.5 76 402.5 77 225.5 78 128.0 79 90.5 80 55.0 81 23.5 82 17.5 83 13.0 84 9.0 85 6.5 86 2.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020525140318307558 2 0.01619265687380992 3 3.790923013935433E-4 4 0.08318368213435465 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.008746200953579607 12-13 0.0 14-15 0.0 16-17 6.227944951465354E-4 18-19 8.123406458433071E-5 20-21 0.0 22-23 1.0831208611244095E-4 24-25 0.0037638449924073227 26-27 0.0040346252076884255 28-29 0.001272667011821181 30-31 1.8954615069677165E-4 32-33 6.498725166746457E-4 34-35 0.0031410504972607875 36-37 0.08686629306217764 38-39 0.0976433456303655 40-41 0.07982600746486898 42-43 0.08329199422046708 44-45 0.082290107423927 46-47 0.08066542613224038 48-49 0.07608924049398975 50-51 0.07543936797731512 52-53 0.05903008693128031 54-55 0.04906537500893574 56-57 0.028161142389234645 58-59 0.023395410600287245 60-61 0.014026415151561101 62-63 0.0020308516146082677 64-65 0.007121519661892992 66-67 0.16303676762075173 68-69 0.7453225425612342 70-71 0.9105526299257628 72-73 0.3719978597531784 74-75 0.0568909232305596 76-77 0.20628036800114374 78-79 0.01489291184046063 80-81 0.003547220820182441 82-83 0.0023287098514174803 84-85 1.8954615069677165E-4 86-87 0.002437021937529921 88-89 6.769505382027559E-4 90-91 0.004359561466025748 92-93 0.004494951573666299 94-95 0.08231718544545512 96-97 0.03910066308659118 98-99 0.014297195366842205 100-101 0.013647322850167558 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1846516.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.337264289374254 #Duplication Level Percentage of deduplicated Percentage of total 1 69.26927189911852 27.941329277272686 2 13.870591176780703 11.190034042953316 3 5.939429074467072 7.187409609143154 4 3.2231648048261343 5.200546023219246 5 1.9296251584943858 3.8917899998806855 6 1.2627375932028462 3.0561228015090935 7 0.8770805643820182 2.47653213699857 8 0.6124698862914075 1.97642877380956 9 0.4693123382544639 1.7037698240191061 >10 2.2456392264774605 16.153176783638482 >50 0.1713873275320146 4.810555435068288 >100 0.12054434167698344 8.920089831547056 >500 0.006559956372058667 1.7514471575215118 >1k 0.0017766548507658889 1.4655573536467026 >5k 0.0 0.0 >10k+ 4.099972732536667E-4 2.2752109497726805 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16863 0.9132333540570458 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14139 0.7657122927719012 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10270 0.5561825621873843 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4911 0.2659603274490987 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3422 0.18532197933838646 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2908 0.15748577320748913 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2723 0.14746690524208836 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1940 0.1050627235290677 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.7078021528110234E-5 0.0 12-13 0.0 0.0 0.0 1.0831208611244094E-4 0.0 14-15 0.0 0.0 0.0 1.0831208611244094E-4 0.0 16-17 0.0 0.0 0.0 1.0831208611244094E-4 0.0 18-19 0.0 0.0 0.0 1.6246812916866142E-4 0.0 20-21 0.0 0.0 0.0 3.2493625833732284E-4 0.0 22-23 0.0 0.0 0.0 7.040285597308661E-4 0.0 24-25 0.0 0.0 0.0 0.0011372769041806299 0.0 26-27 0.0 0.0 0.0 0.0023828658944737005 0.0 28-29 0.0 0.0 0.0 0.004874043875059842 0.0 30-31 0.0 0.0 0.0 0.012266343752233937 0.0 32-33 0.0 0.0 0.0 0.027240489657278898 0.0 34-35 0.0 0.0 0.0 0.05044635410686937 0.0 36-37 0.0 0.0 0.0 0.08448342716770393 0.0 38-39 0.0 0.0 0.0 0.14069739986006077 0.0 40-41 0.0 0.0 0.0 0.20319347354693923 0.0 42-43 0.0 0.0 0.0 0.26476889450186186 0.0 44-45 0.0 0.0 0.0 0.3347385021304987 0.0 46-47 0.0 0.0 0.0 0.42155063914962015 0.0 48-49 0.0 0.0 0.0 0.5178942397466364 0.0 50-51 0.0 0.0 0.0 0.6241754742444691 0.0 52-53 0.0 0.0 0.0 0.7263950055130852 0.0 54-55 0.0 0.0 0.0 0.8375232058644496 0.0 56-57 0.0 0.0 0.0 0.9615405444631945 0.0 58-59 0.0 0.0 0.0 1.0870200962244572 0.0 60-61 0.0 0.0 0.0 1.2237641049414139 0.0 62-63 0.0 0.0 0.0 1.352465941264522 2.7078021528110234E-5 64-65 0.0 0.0 0.0 1.4869624741946454 5.415604305622047E-5 66-67 0.0 0.0 0.0 1.633508726704778 5.415604305622047E-5 68-69 0.0 0.0 0.0 1.7947854229262026 5.415604305622047E-5 70-71 0.0 0.0 0.0 2.0044234655968323 5.415604305622047E-5 72-73 0.0 0.0 0.0 2.2382421814920637 5.415604305622047E-5 74-75 0.0 0.0 0.0 2.486628872969419 5.415604305622047E-5 76-77 0.0 0.0 0.0 2.760035656338748 5.415604305622047E-5 78-79 0.0 0.0 0.0 3.0826432048246537 5.415604305622047E-5 80-81 0.0 0.0 0.0 3.4353885912713458 5.415604305622047E-5 82-83 0.0 0.0 0.0 3.800481555534856 5.415604305622047E-5 84-85 0.0 0.0 0.0 4.171910776835944 5.415604305622047E-5 86-87 0.0 0.0 0.0 4.54780787168917 5.415604305622047E-5 88-89 0.0 0.0 0.0 4.953057541878868 5.415604305622047E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 12620 0.0 33.79913 1 TATCGCG 60 0.0039923987 31.600233 7 GTATCAA 18540 0.0 29.967623 1 GTACATG 15005 0.0 23.652159 1 TATCAAC 23440 0.0 23.616335 2 ATCAACG 23835 0.0 23.181385 3 TCAACGC 23890 0.0 23.131779 4 CAACGCA 24190 0.0 22.847792 5 TACATGG 15340 0.0 22.727999 2 AACGCAG 24630 0.0 22.458876 6 GAGTACT 12690 0.0 22.259089 12-13 ACATGGG 15305 0.0 21.877615 3 AGTACTT 13450 0.0 21.494644 12-13 CATTCCG 465 0.0 21.40661 9 TTAGGCA 600 0.0 21.345766 4 TATATCG 135 3.851377E-4 21.06682 5 GTACTTT 13995 0.0 20.268145 14-15 ACGCAGA 27665 0.0 19.875078 7 CGCAGAG 27815 0.0 19.716774 8 CATGGGG 4125 0.0 19.318996 4 >>END_MODULE