FastQCFastQC Report
Fri 27 May 2016
SRR939439_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939439_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2300205
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95290.41426742399047045No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76050.3306227053675651No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49140.21363313269904205No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT30460.1324229796909406No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25220.10964240143813268No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA169000.028.2698331
GTACATG131100.027.903611
GGTATCA122850.027.3367021
TACATGG134750.026.9691522
ACATGGG134500.025.607083
TATCAAC203750.023.5339972
ATCAACG207800.023.144173
GAGTACT97200.022.91353612-13
TCAACGC209600.022.7916414
CAACGCA211350.022.4117245
CATGGGA88650.022.3001444
AACGCAG213750.022.2417036
GTATAGG7450.022.1365381
CATGGGG46850.021.966724
GTACTTT104800.021.29188314-15
AGTACTT101250.020.6487912-13
ACGCAGA236600.019.6937567
CGCAGAG237900.019.5965278
ATGGGAT38400.019.4898725
ACTTTTT129450.018.90133916-17
Produced by FastQC (version 0.11.5)