FastQCFastQC Report
Fri 27 May 2016
SRR939439_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939439_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2300205
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133230.5792092443934345No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119830.5209535671820555No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80490.3499253327420817No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT43940.1910264519901487No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39370.17115865759790974No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32860.14285683232581442No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26620.11572881547514244No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT25680.11164222319315018No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA128900.029.7454431
GTATCAA176450.026.6180841
GTACATG149200.025.031231
TACATGG150650.024.5951292
ACATGGG150600.023.30933
GAGTACT113750.021.86471712-13
TATCAAC218100.021.3772072
ATCAACG218400.021.3224373
TCAACGC220200.021.1266274
CAACGCA222350.020.879735
CATGGGA100800.020.7729284
AACGCAG225450.020.6136446
GTACTTT119750.020.51205614-15
AGTACTT118850.019.67088712-13
ATGGGAT43550.019.0364465
CGTACAC2001.31553825E-518.949423
AGAGTAC203950.018.39659510-11
CATGGGG49050.018.254034
ACTTTTT137700.018.2338316-17
CGCAGAG254400.018.1561378
Produced by FastQC (version 0.11.5)