##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939439_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2300205 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.976586869431202 31.0 31.0 31.0 30.0 34.0 2 31.231470238522217 31.0 31.0 34.0 30.0 34.0 3 31.221462000126074 31.0 31.0 34.0 28.0 34.0 4 35.16431796296417 35.0 35.0 37.0 33.0 37.0 5 34.79614990837773 35.0 35.0 37.0 32.0 37.0 6 34.760727413426196 35.0 35.0 37.0 32.0 37.0 7 34.620054734251944 35.0 35.0 37.0 30.0 37.0 8 34.63677759156249 35.0 35.0 37.0 30.0 37.0 9 35.97135037964008 37.0 35.0 39.0 30.0 39.0 10-11 35.83228386165581 37.0 35.0 39.0 30.0 39.0 12-13 35.79827602322402 37.0 35.0 39.0 30.0 39.0 14-15 36.63830354251034 38.0 35.5 40.0 31.0 41.0 16-17 36.658046782786755 38.0 35.5 40.0 31.0 41.0 18-19 36.63797922359094 38.0 35.0 40.0 30.0 41.0 20-21 36.54055616781983 38.0 35.0 40.0 30.0 41.0 22-23 36.401644853393506 38.0 35.0 40.0 30.0 41.0 24-25 36.18136122649938 38.0 34.0 40.0 29.5 41.0 26-27 35.80445112500843 38.0 34.0 40.0 28.0 41.0 28-29 35.5355742205586 38.0 34.0 40.0 27.0 41.0 30-31 35.30836208077106 38.0 34.0 40.0 27.0 41.0 32-33 35.07907338693725 37.5 33.0 40.0 27.0 41.0 34-35 35.01815272986538 38.0 33.0 40.0 26.5 41.0 36-37 35.00162789838297 38.0 33.0 40.0 26.0 41.0 38-39 34.96063698670336 38.0 33.0 40.0 26.0 41.0 40-41 34.740551603009294 38.0 33.0 40.0 25.5 41.0 42-43 34.423823746144365 37.5 33.0 40.0 24.5 41.0 44-45 34.20575644344743 37.0 33.0 40.0 24.0 41.0 46-47 33.84830373814508 37.0 33.0 40.0 23.5 41.0 48-49 33.509034194778295 37.0 32.0 40.0 22.5 41.0 50-51 33.235945057071 37.0 32.0 40.0 21.0 41.0 52-53 32.877478529087625 37.0 31.0 39.5 19.0 41.0 54-55 32.65763421086382 36.0 31.0 39.0 18.5 40.5 56-57 32.264396651602794 36.0 31.0 39.0 17.5 40.0 58-59 31.846719096776155 35.5 30.0 39.0 14.0 40.0 60-61 31.547174708341213 35.0 30.0 39.0 13.0 40.0 62-63 31.17716768722788 35.0 29.0 38.0 10.0 40.0 64-65 30.749205179538343 34.5 29.0 38.0 8.0 40.0 66-67 29.038619818668337 33.5 25.0 37.5 4.5 39.5 68-69 25.349285824524337 30.0 15.5 35.0 2.0 39.0 70-71 24.74044443864786 29.5 14.0 35.0 2.0 38.0 72-73 26.58827930554016 31.0 21.5 35.0 2.0 38.0 74-75 27.21086316219641 32.0 23.5 35.0 2.0 38.0 76-77 27.25988227136277 31.5 25.0 34.5 2.0 36.5 78-79 28.09322016950663 33.0 26.0 35.0 2.0 37.0 80-81 27.949098015176908 33.0 26.0 35.0 2.0 37.0 82-83 27.5487213096224 33.0 26.0 35.0 2.0 36.0 84-85 27.201907221312883 32.0 26.0 35.0 2.0 36.0 86-87 26.765899561126073 32.0 25.0 35.0 2.0 35.5 88-89 26.280899745892214 32.0 24.5 34.0 2.0 35.0 90-91 25.827266048026154 32.0 23.5 34.0 2.0 35.0 92-93 25.27136103086464 31.5 21.0 34.0 2.0 35.0 94-95 23.89389489197702 31.0 10.5 34.0 2.0 35.0 96-97 23.101454435582916 30.0 4.5 34.0 2.0 35.0 98-99 22.78695551048711 30.0 2.0 34.0 2.0 35.0 100-101 21.537283198671425 29.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.39308273206611943 1101 2 0.2043987786337702 1101 3 0.35290184331368124 1101 4 0.32167422694559633 1101 5 0.3227764548950063 1101 6 0.4597281629084691 1101 7 0.505079960483009 1101 8 0.43597240184279684 1101 9 0.6205094661556885 1101 10-11 0.725835672267408 1101 12-13 0.5441981506566052 1101 14-15 0.547904491218631 1101 16-17 0.5701672596488692 1101 18-19 0.7488726082379102 1101 20-21 0.6403673333302606 1101 22-23 0.7283063095121562 1101 24-25 0.6521698498608401 1101 26-27 0.6784364199435657 1101 28-29 0.5350131234869977 1101 30-31 0.7852106191569064 1101 32-33 0.7035880102932097 1101 34-35 0.8207598365189668 1101 36-37 0.6652810902702626 1101 38-39 0.6493080686708552 1101 40-41 0.975016087127031 1101 42-43 1.012935882710515 1101 44-45 0.7002407528050583 1101 46-47 0.8119166387887802 1101 48-49 0.6603140220741963 1101 50-51 0.7879535101420956 1101 52-53 1.0107415762875434 1101 54-55 0.8997414007271658 1101 56-57 0.7042196932975386 1101 58-59 0.8954172027981429 1101 60-61 0.6544768456870393 1101 62-63 0.6137233730792637 1101 64-65 0.26918511565966696 1101 66-67 -2.616284381021906 1101 68-69 -7.63505931200427 1101 70-71 -11.349581779716274 1101 72-73 -11.04017093665594 1101 74-75 -9.495988900541743 1101 76-77 -4.47881021209999 1101 78-79 -1.4068244530645622 1101 80-81 -0.809047028474442 1101 82-83 -0.5249419992499895 1101 84-85 -0.6423159304673369 1101 86-87 -0.5892550158482663 1101 88-89 -0.49013686217712404 1101 90-91 -0.5825095311427155 1101 92-93 -1.2788020790925891 1101 94-95 -4.727431163254696 1101 96-97 -1.8998549437735015 1101 98-99 -1.3396650155309757 1101 100-101 -0.9834556077873522 1102 1 0.4205501340400062 1102 2 0.28607227614629593 1102 3 0.462518708933505 1102 4 0.23018592886890588 1102 5 0.5123015872579373 1102 6 0.5348849923542787 1102 7 0.5136286999437587 1102 8 0.49148141979292603 1102 9 0.6023443310200776 1102 10-11 0.6541833105296249 1102 12-13 0.6891214673871744 1102 14-15 0.6710205993459226 1102 16-17 0.7165581263859906 1102 18-19 0.6529035317913809 1102 20-21 0.8134101006716605 1102 22-23 0.853371440946951 1102 24-25 0.7133410437619006 1102 26-27 0.6693325579946006 1102 28-29 0.7532994750516551 1102 30-31 0.5765632793684929 1102 32-33 0.5425859672074793 1102 34-35 0.74662359536201 1102 36-37 0.8012747918328884 1102 38-39 0.6968753104522492 1102 40-41 0.9117592187345238 1102 42-43 0.9759413011759435 1102 44-45 0.7820904796789279 1102 46-47 0.7754521404873387 1102 48-49 0.6782428630078172 1102 50-51 0.6358292414205167 1102 52-53 0.6843512881701486 1102 54-55 0.7460601455981575 1102 56-57 0.5912212799492309 1102 58-59 0.4097038234062893 1102 60-61 0.2682029394398917 1102 62-63 0.32812665752461356 1102 64-65 0.2631481586002984 1102 66-67 -2.706990367592052 1102 68-69 -7.637037192869695 1102 70-71 -11.145985026566027 1102 72-73 -9.137871980579934 1102 74-75 -7.415955584380985 1102 76-77 -3.826317844299915 1102 78-79 -1.0901759037552914 1102 80-81 -0.44917896258580825 1102 82-83 -0.34808731739391874 1102 84-85 -0.24475548820213433 1102 86-87 -0.16498187384910779 1102 88-89 -0.20753083231329583 1102 90-91 -0.16778053078027 1102 92-93 -0.49286810459767594 1102 94-95 -4.108796861501858 1102 96-97 -1.0087298837820917 1102 98-99 -0.13590099134525957 1102 100-101 -0.019760912232385408 1103 1 0.424779163504585 1103 2 0.2777109616490243 1103 3 0.36436204430397723 1103 4 0.25011646164756485 1103 5 0.44521817232106997 1103 6 0.4886995545149375 1103 7 0.5571064735004398 1103 8 0.39173378118293556 1103 9 0.7047542112221521 1103 10-11 0.635403977499891 1103 12-13 0.4672233652297777 1103 14-15 0.7979286473028395 1103 16-17 0.6836582356364076 1103 18-19 0.6899920435080702 1103 20-21 0.7053368368212816 1103 22-23 0.6428440540140556 1103 24-25 0.8603869761304566 1103 26-27 0.6973949321973407 1103 28-29 0.7207567718234529 1103 30-31 0.7194509708811765 1103 32-33 0.7604612117231326 1103 34-35 0.7730805429100087 1103 36-37 0.6769287016057319 1103 38-39 0.7773684474966416 1103 40-41 0.9386190714470288 1103 42-43 0.8824779213030354 1103 44-45 0.7605223704190678 1103 46-47 0.692868084913556 1103 48-49 0.4940936712957509 1103 50-51 0.5095155228241097 1103 52-53 0.6176427579068502 1103 54-55 0.6567883362177227 1103 56-57 0.6076386330687669 1103 58-59 0.8530198449920903 1103 60-61 0.7628663632017343 1103 62-63 0.5210244364140344 1103 64-65 0.43079121647942387 1103 66-67 -3.0297209437877335 1103 68-69 -7.952575056516675 1103 70-71 -11.452774892940731 1103 72-73 -9.378537322713079 1103 74-75 -7.824601410094711 1103 76-77 -3.92846462683967 1103 78-79 -1.0761821756260233 1103 80-81 -0.3400167339716411 1103 82-83 -0.11236950910362964 1103 84-85 -0.24701452967717685 1103 86-87 -0.03635041822294838 1103 88-89 -0.10116150926476308 1103 90-91 -0.10697636038737102 1103 92-93 -0.08844658910794934 1103 94-95 0.8514883693987834 1103 96-97 0.9561156082813262 1103 98-99 0.7002956428923568 1103 100-101 0.5398239243757068 1104 1 0.3323003158417279 1104 2 0.11962831781283967 1104 3 0.2686129906888439 1104 4 0.1537710059016888 1104 5 0.4266103674139501 1104 6 0.5067728907969311 1104 7 0.47347462261762274 1104 8 0.3749581271271367 1104 9 0.5671327032347762 1104 10-11 0.5022424852997176 1104 12-13 0.547263730099921 1104 14-15 0.734929783139961 1104 16-17 0.633792415160606 1104 18-19 0.6081181401255122 1104 20-21 0.6390805477951389 1104 22-23 0.4471348335757668 1104 24-25 0.6210480111328209 1104 26-27 0.8240796257988521 1104 28-29 0.9344555576345996 1104 30-31 0.7771800824917747 1104 32-33 0.7147948919230416 1104 34-35 0.6780462077924838 1104 36-37 0.8646618651867257 1104 38-39 0.7130237711780794 1104 40-41 0.8020551072819941 1104 42-43 0.939715654817185 1104 44-45 0.9376037013817893 1104 46-47 0.8262619738293893 1104 48-49 0.9133286551959614 1104 50-51 0.9908753635030862 1104 52-53 0.9054365841880383 1104 54-55 0.8300703241430298 1104 56-57 0.8971530130354992 1104 58-59 1.1553289714784256 1104 60-61 0.7478390890881279 1104 62-63 0.9465505644656496 1104 64-65 0.7250565828540907 1104 66-67 -2.78189752672926 1104 68-69 -7.333828076505423 1104 70-71 -8.569174607696038 1104 72-73 -7.794502246730602 1104 74-75 -6.956280661143342 1104 76-77 -3.615954073311883 1104 78-79 -0.6171267229359181 1104 80-81 0.24749677016183824 1104 82-83 0.3748803686361235 1104 84-85 0.36741507150025754 1104 86-87 0.45527599653405204 1104 88-89 0.5371257108732088 1104 90-91 0.4090511252349991 1104 92-93 0.37360044179395047 1104 94-95 1.2746594905062487 1104 96-97 1.508007779126654 1104 98-99 0.9870754540886537 1104 100-101 0.8027159532413961 1105 1 0.49084256190895914 1105 2 0.2958963335291678 1105 3 0.41619866166481145 1105 4 0.22777001271658293 1105 5 0.3326285113667993 1105 6 0.47676649393727644 1105 7 0.5570554856548853 1105 8 0.4303879892332674 1105 9 0.7649784756931766 1105 10-11 0.773218483131707 1105 12-13 0.7654665848719375 1105 14-15 0.9449448473721134 1105 16-17 0.8341579458261847 1105 18-19 0.8829762038701148 1105 20-21 0.9890610476671569 1105 22-23 1.0085389187175124 1105 24-25 0.9237535863588562 1105 26-27 0.9919282732667583 1105 28-29 1.1030166058843847 1105 30-31 1.0809472502336135 1105 32-33 1.0753316703617273 1105 34-35 1.2666985642416861 1105 36-37 1.1208436831827058 1105 38-39 0.9679051912721732 1105 40-41 1.0966564848043205 1105 42-43 1.1296404037430037 1105 44-45 1.0026293640174515 1105 46-47 1.0821024848305711 1105 48-49 0.9648516290625722 1105 50-51 1.1108738654026595 1105 52-53 1.2447553836109364 1105 54-55 1.3693015007519804 1105 56-57 1.3199453554352587 1105 58-59 1.3927932948590254 1105 60-61 1.4166322991012272 1105 62-63 1.433738812466391 1105 64-65 1.3750770320710366 1105 66-67 1.927205755030581 1105 68-69 -4.940594964046994 1105 70-71 -5.270463528922093 1105 72-73 -1.652115758136386 1105 74-75 1.008173547723107 1105 76-77 1.254917188901981 1105 78-79 1.1970748019434296 1105 80-81 1.0041969367488583 1105 82-83 1.2493297692718386 1105 84-85 1.3408546706751636 1105 86-87 1.3761406662299187 1105 88-89 1.3813809410469986 1105 90-91 1.383392650958303 1105 92-93 1.4696613867390518 1105 94-95 2.1669040902995214 1105 96-97 2.2918943802244165 1105 98-99 2.010099184379179 1105 100-101 1.8235778048730928 1106 1 0.005648137848723422 1106 2 0.12029739919307758 1106 3 0.1403514579703362 1106 4 0.11404972264213598 1106 5 0.4007085783824138 1106 6 0.32765852568294207 1106 7 0.3548698360014839 1106 8 0.25100054238589564 1106 9 0.436601529984614 1106 10-11 0.4922014108512158 1106 12-13 0.40146716143281935 1106 14-15 0.400180014281176 1106 16-17 0.4023924804067107 1106 18-19 0.2683201088632714 1106 20-21 0.407463077019834 1106 22-23 0.3833636120470203 1106 24-25 0.3993672592906776 1106 26-27 0.6678725097381673 1106 28-29 0.5092266888542767 1106 30-31 0.5786969218978371 1106 32-33 0.7099148926761174 1106 34-35 0.6854436827273034 1106 36-37 0.6765057087408195 1106 38-39 0.5382762321002872 1106 40-41 0.76104324267029 1106 42-43 0.8173120774012759 1106 44-45 0.8045623330610923 1106 46-47 0.8172895322162645 1106 48-49 0.8064954501681783 1106 50-51 0.8241692295167908 1106 52-53 0.9907671845197967 1106 54-55 0.8116286674804698 1106 56-57 0.8053508999208816 1106 58-59 0.8317382563099969 1106 60-61 0.8722622656317718 1106 62-63 0.9134269178147463 1106 64-65 0.7601901946845224 1106 66-67 1.5413698996122172 1106 68-69 -5.2939288225995575 1106 70-71 -6.430736610462969 1106 72-73 -2.2943090529752794 1106 74-75 0.5561860355479027 1106 76-77 0.8631003995317066 1106 78-79 0.5940689103410044 1106 80-81 0.7601198162721197 1106 82-83 0.779240414137778 1106 84-85 0.7755992061084314 1106 86-87 0.845066055888001 1106 88-89 0.744096159215097 1106 90-91 0.6258624678190792 1106 92-93 0.7345871287071049 1106 94-95 1.625481038309605 1106 96-97 1.5623150070588103 1106 98-99 1.411822331292143 1106 100-101 1.4823528313479777 1107 1 0.031107473052244927 1107 2 -0.020921585988673996 1107 3 -0.05112752944271293 1107 4 0.007172692370126299 1107 5 0.17749496136960374 1107 6 0.1165602699183097 1107 7 0.14535850594548805 1107 8 0.02557038809376877 1107 9 0.18442111923298654 1107 10-11 0.22435249773916865 1107 12-13 0.2749425054272834 1107 14-15 0.3049474573341229 1107 16-17 0.14838927268181834 1107 18-19 0.2195942021495796 1107 20-21 0.11478925714530419 1107 22-23 0.13460170311197572 1107 24-25 0.3516885557311369 1107 26-27 0.2700889071728554 1107 28-29 0.31882691347581726 1107 30-31 0.34026479144579724 1107 32-33 0.25215705881338835 1107 34-35 0.28096867031997874 1107 36-37 0.3551109108986523 1107 38-39 0.25709476567713807 1107 40-41 0.2878005690524148 1107 42-43 0.2670167255568643 1107 44-45 0.2190269130595226 1107 46-47 0.12531355255029553 1107 48-49 0.2188573885638263 1107 50-51 0.060194789384730996 1107 52-53 0.30021707617129323 1107 54-55 0.32712539402860585 1107 56-57 0.30383855801134274 1107 58-59 0.22139098171078686 1107 60-61 0.2610359898836627 1107 62-63 0.4874214272040689 1107 64-65 0.24281558791983215 1107 66-67 0.941710017274378 1107 68-69 -5.681846153916641 1107 70-71 -5.879508805746969 1107 72-73 -2.349025359510968 1107 74-75 0.27682285999349077 1107 76-77 0.1881634772259595 1107 78-79 0.24276857288039366 1107 80-81 0.10521820007227234 1107 82-83 0.2591548416589511 1107 84-85 0.1621386794287183 1107 86-87 0.27544495401803104 1107 88-89 0.23873032101905522 1107 90-91 0.25868922177365405 1107 92-93 0.3768760806400202 1107 94-95 1.2879061398601088 1107 96-97 1.5121087673160254 1107 98-99 1.064390096112021 1107 100-101 0.8673916164773345 1108 1 0.02818502415628288 1108 2 0.00617505539609553 1108 3 0.07992454255546377 1108 4 0.008567406574570668 1108 5 0.11976054837732164 1108 6 0.13962179645565698 1108 7 0.20465362862367442 1108 8 0.04569569947536678 1108 9 0.15495107861612212 1108 10-11 0.22187160962972285 1108 12-13 0.229695130540982 1108 14-15 0.1273912789886964 1108 16-17 0.03451613228741479 1108 18-19 0.19032745397385042 1108 20-21 0.20815331164816087 1108 22-23 0.24045959881430434 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0.8195854725167919 1108 92-93 0.7538499782107273 1108 94-95 1.5256185524483605 1108 96-97 1.661972033140259 1108 98-99 1.4175055447688365 1108 100-101 1.3930027823866986 1109 1 0.0332331116994844 1109 2 0.08220213024509704 1109 3 0.056740527090660464 1109 4 -0.05872162182471641 1109 5 0.04035086552086398 1109 6 0.2543909765661425 1109 7 0.15376718985436355 1109 8 0.01902895652719394 1109 9 0.22161761814694358 1109 10-11 0.2118940681762851 1109 12-13 0.18591995565768116 1109 14-15 -0.05834441322305395 1109 16-17 0.1272761115769825 1109 18-19 0.12947181704075916 1109 20-21 0.2473680444038564 1109 22-23 0.16851151854964286 1109 24-25 0.2950826001687403 1109 26-27 0.2830031417905232 1109 28-29 0.4100409081426051 1109 30-31 0.3860596511067129 1109 32-33 0.38431657316326806 1109 34-35 0.3654776855342874 1109 36-37 0.5119296238735629 1109 38-39 0.383373330307343 1109 40-41 0.26372951378629494 1109 42-43 0.38851272419815785 1109 44-45 0.3812947222388203 1109 46-47 0.4494298980002043 1109 48-49 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0.6295285318014869 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9.0 3 37.0 4 70.0 5 179.0 6 466.0 7 1081.0 8 2086.0 9 3699.0 10 6025.0 11 9059.0 12 11882.0 13 15355.0 14 18526.0 15 23225.0 16 27770.0 17 31689.0 18 28799.0 19 24156.0 20 23173.0 21 23660.0 22 25300.0 23 28522.0 24 34600.0 25 43014.0 26 54095.0 27 67724.0 28 82537.0 29 99201.0 30 116617.0 31 136220.0 32 156971.0 33 182047.0 34 211030.0 35 241528.0 36 255182.0 37 216468.0 38 92341.0 39 5861.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.45410313754122 20.446699456921372 14.888720845235417 22.21047656030199 2 16.110752865374053 21.5513871502013 38.90929899160226 23.428560992822376 3 19.611512886894864 25.754747946378693 30.171136920404923 24.462602246321524 4 13.002536730421854 17.42762058164381 36.548003330137966 33.02183935779637 5 12.988723169215936 38.85261431273675 33.756933627162475 14.401728890884849 6 31.329424986033853 36.75607174143174 17.02070032888373 14.893802943650675 7 28.105842740103597 33.90623879175986 20.343404174845286 17.644514293291248 8 26.663797357192077 35.07074369458375 19.16159646640191 19.103862481822272 9 26.74131218739199 16.71611878071737 20.534343678063475 36.008225353827164 10-11 25.22831660656333 27.30256651037625 26.391734649737742 21.077382233322684 12-13 26.240639421268973 24.775161344314963 27.4467710486674 21.53742818574866 14-15 22.982690673222603 25.941426959770975 26.482856962748976 24.593025404257446 16-17 21.474711856389785 29.341076433186092 27.19578332043952 21.988428389984605 18-19 21.290517375551367 27.34131065922495 30.23538096484371 21.132791000379967 20-21 22.484054128254304 26.17824901144616 30.279560047621874 21.05813681267766 22-23 21.875441536732595 26.362150330079277 30.26232444499512 21.50008368819301 24-25 21.748996285113716 26.613301857877886 30.438069650313775 21.19963220669462 26-27 21.535650743034022 26.66660145487507 30.526335238604513 21.271412563486397 28-29 21.140261033982945 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28.565943513286335 21.757230518627317 54-55 21.70333333405838 27.459945373578897 28.730943886001874 22.105777406360843 56-57 21.896829462832667 27.69934042458808 28.381335137895014 22.022494974684236 58-59 22.04809454550198 27.32008026627881 28.54711947979103 22.084705708428174 60-61 21.923767142718944 27.584198644126012 28.910050793643894 21.58198341951115 62-63 21.792831803005008 27.435613174735668 28.983592445742907 21.787962576516417 64-65 21.614724122691747 27.582622328392002 28.7597069592292 22.042946589687055 66-67 21.26075790961926 27.577209922045064 28.953161326126793 22.20887084220888 68-69 21.322288943387054 27.179509547200325 29.49134420924607 22.006857300166555 70-71 20.99960048275549 27.253153301194978 29.713661756546344 22.03358445950319 72-73 21.709482945608848 27.39534817815089 28.643027880629617 22.252140995610645 74-75 21.337588145740824 27.883846930380567 28.525486748667163 22.253078175211453 76-77 21.57550254080352 27.712346344108322 28.27605383451101 22.436097280577147 78-79 21.492816546725056 27.667164635949092 28.34661879436941 22.493400022956443 80-81 21.517547713290075 27.897354610297537 28.091405358252636 22.493692318159756 82-83 21.664612501929177 27.81036907579977 28.03361004780009 22.49140837447097 84-85 21.452457498353407 27.7484180757802 27.94437887057893 22.854745555287465 86-87 21.442334194996786 27.744237245250638 28.134007361092632 22.679421198659945 88-89 21.425753335325926 27.656875798168628 27.85696818194169 23.06040268456376 90-91 21.354151608618047 27.665456660753225 28.01591564637858 22.964476084250148 92-93 21.531978718894862 27.652915826599205 28.023824403710606 22.791281050795327 94-95 20.97430965756087 27.353822395975858 28.403192577783898 23.26867536867938 96-97 20.795855976262594 27.232370762355952 28.536681926699114 23.43509133468234 98-99 21.02670696429868 27.306624195014802 27.952199390665715 23.714469450020808 100-101 20.87169166207975 27.13891432342821 28.17262182709557 23.816772187396467 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2763.0 1 1878.5 2 1071.5 3 1259.0 4 1262.0 5 1225.0 6 1794.0 7 3163.5 8 4556.5 9 5306.0 10 5067.0 11 4343.0 12 4081.5 13 4057.5 14 4104.0 15 4265.0 16 4417.5 17 4568.5 18 4831.0 19 5152.0 20 5637.5 21 6284.5 22 7105.5 23 8309.5 24 9874.0 25 11800.0 26 14494.5 27 17594.0 28 20243.0 29 23425.5 30 27326.5 31 32100.5 32 38337.0 33 44243.0 34 49626.5 35 54875.5 36 61580.5 37 67398.5 38 69319.5 39 70001.0 40 70065.5 41 70367.5 42 71464.0 43 73011.0 44 75314.0 45 77467.0 46 78935.0 47 82643.0 48 89556.0 49 88426.5 50 80393.0 51 75313.5 52 72524.0 53 71689.0 54 70121.5 55 66007.5 56 60606.5 57 55119.0 58 50128.0 59 44533.0 60 37937.5 61 31417.5 62 25348.5 63 19576.5 64 14603.5 65 10899.5 66 8008.0 67 5945.0 68 4187.0 69 2799.0 70 2010.0 71 1512.5 72 1267.0 73 1097.5 74 845.0 75 611.0 76 418.0 77 244.5 78 150.0 79 101.5 80 64.5 81 42.5 82 28.0 83 19.5 84 12.0 85 6.5 86 2.5 87 0.5 88 0.5 89 0.5 90 1.5 91 1.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023389219656508874 2 0.018172293339071954 3 0.0 4 0.0 5 8.694877195728207E-5 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.8258350886116673E-4 18-19 8.260133335941796E-4 20-21 6.521157896796154E-5 22-23 0.0 24-25 0.0 26-27 3.2605789483980776E-4 28-29 0.016933273338680683 30-31 0.004151803860960219 32-33 0.007760177897187424 34-35 0.009542627722311707 36-37 0.09840427266265399 38-39 0.10118663336528701 40-41 0.11651135442275797 42-43 0.1009692614353938 44-45 0.09599144424083941 46-47 0.09727393862720932 48-49 0.10027367125973555 50-51 0.09816516353977145 52-53 0.07621059862055773 54-55 0.06523331616095088 56-57 0.03682280492390896 58-59 0.015650778952310773 60-61 0.009238307020461219 62-63 0.0019563473690388466 64-65 0.00502129158053304 66-67 0.43350483978601906 68-69 0.825426429383468 70-71 0.9218526174840938 72-73 0.2863875176342978 74-75 0.152660306363998 76-77 0.014868240004695235 78-79 0.008564454037792284 80-81 0.0014563919302844747 82-83 0.0 84-85 0.0 86-87 1.3042315793592308E-4 88-89 7.608017546262181E-4 90-91 0.003412739299323321 92-93 0.0018476614040922438 94-95 0.22854484708971592 96-97 0.23608765305701013 98-99 0.01382485474120785 100-101 0.007955812634091309 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2300205.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.29192468704493 #Duplication Level Percentage of deduplicated Percentage of total 1 73.21875617198175 36.090934149084305 2 14.082683698351056 13.883251685011913 3 5.206199888176486 7.698708383810914 4 2.5235549105426065 4.975635143763543 5 1.3490989014151542 3.3249840721965422 6 0.8206278072653403 2.427019444309079 7 0.5492592535831479 1.8951832032898124 8 0.3782656041250128 1.4916351736183748 9 0.26280063735700626 1.1658554301878061 >10 1.415641469256921 12.94368061995603 >50 0.12030606437642079 4.10764097545767 >100 0.06807977062959698 6.171175601055722 >500 0.0029424935284258842 0.9428994566778861 >1k 0.001515829999492025 1.41538205395033 >5k 8.91664705583544E-5 0.35376859771593344 >10k+ 1.783329411167088E-4 1.112246009914202 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13323 0.5792092443934345 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11983 0.5209535671820555 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8049 0.3499253327420817 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4394 0.1910264519901487 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3937 0.17115865759790974 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3286 0.14285683232581442 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2662 0.11572881547514244 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2568 0.11164222319315018 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 4.3474385978641034E-5 7 0.0 0.0 0.0 0.0 4.3474385978641034E-5 8 0.0 0.0 0.0 0.0 4.3474385978641034E-5 9 0.0 0.0 0.0 0.0 4.3474385978641034E-5 10-11 0.0 0.0 0.0 0.0 4.3474385978641034E-5 12-13 0.0 0.0 0.0 0.0 4.3474385978641034E-5 14-15 0.0 0.0 0.0 4.3474385978641034E-5 1.304231579359231E-4 16-17 0.0 0.0 0.0 1.304231579359231E-4 1.7389754391456414E-4 18-19 0.0 0.0 0.0 1.9563473690388465E-4 2.1737192989320516E-4 20-21 0.0 0.0 0.0 2.391091228825257E-4 2.1737192989320516E-4 22-23 0.0 0.0 0.0 3.912694738077693E-4 2.8258350886116673E-4 24-25 0.0 0.0 0.0 7.390645616368976E-4 3.477950878291283E-4 26-27 0.0 0.0 0.0 0.0016085522812097183 3.912694738077693E-4 28-29 0.0 0.0 0.0 0.0032171045624194367 3.912694738077693E-4 30-31 0.0 0.0 0.0 0.008390556493877719 3.912694738077693E-4 32-33 0.0 0.0 0.0 0.01984605719924963 3.912694738077693E-4 34-35 0.0 0.0 0.0 0.0378227158014177 3.912694738077693E-4 36-37 0.0 0.0 0.0 0.06766788177575478 3.912694738077693E-4 38-39 0.0 0.0 0.0 0.11279429442158417 4.130066667970898E-4 40-41 0.0 0.0 0.0 0.16207251092837377 4.3474385978641033E-4 42-43 0.0 0.0 0.0 0.21322012603224494 4.3474385978641033E-4 44-45 0.0 0.0 0.0 0.2753232864027337 4.3474385978641033E-4 46-47 0.0 0.0 0.0 0.34970796081218847 4.3474385978641033E-4 48-49 0.0 0.0 0.0 0.42904871522320837 4.3474385978641033E-4 50-51 0.0 0.0 0.0 0.5202579770063973 4.3474385978641033E-4 52-53 0.0 0.0 0.0 0.6052286644016511 4.3474385978641033E-4 54-55 0.0 0.0 0.0 0.6997202423262274 4.3474385978641033E-4 56-57 0.0 0.0 0.0 0.8092322206064242 4.7821824576505136E-4 58-59 0.0 0.0 0.0 0.9161792101138811 4.7821824576505136E-4 60-61 0.0 0.0 0.0 1.0272997406752875 4.7821824576505136E-4 62-63 0.0 4.3474385978641034E-5 0.0 1.1340293582528513 4.7821824576505136E-4 64-65 0.0 4.3474385978641034E-5 0.0 1.248214833025752 4.7821824576505136E-4 66-67 0.0 4.3474385978641034E-5 0.0 1.3688779913094702 4.7821824576505136E-4 68-69 0.0 4.3474385978641034E-5 0.0 1.5123652022319751 4.7821824576505136E-4 70-71 0.0 4.3474385978641034E-5 0.0 1.7030003847483157 4.999554387543719E-4 72-73 0.0 4.3474385978641034E-5 0.0 1.9144163237624472 5.651670177223335E-4 74-75 0.0 4.3474385978641034E-5 0.0 2.140070124184583 5.651670177223335E-4 76-77 0.0 4.3474385978641034E-5 0.0 2.390417375842588 5.651670177223335E-4 78-79 0.0 4.3474385978641034E-5 0.0 2.6783482341791274 5.651670177223335E-4 80-81 0.0 4.3474385978641034E-5 0.0 2.9964068419988656 5.869042107116539E-4 82-83 0.0 4.3474385978641034E-5 0.0 3.325986162102943 6.086414037009745E-4 84-85 0.0 6.521157896796155E-5 0.0 3.646653233081399 6.086414037009745E-4 86-87 0.0 8.694877195728207E-5 0.0 3.9706243573942324 6.303785966902951E-4 88-89 0.0 8.694877195728207E-5 0.0 4.331766081718804 6.521157896796155E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 12890 0.0 29.745443 1 GTATCAA 17645 0.0 26.618084 1 GTACATG 14920 0.0 25.03123 1 TACATGG 15065 0.0 24.595129 2 ACATGGG 15060 0.0 23.3093 3 GAGTACT 11375 0.0 21.864717 12-13 TATCAAC 21810 0.0 21.377207 2 ATCAACG 21840 0.0 21.322437 3 TCAACGC 22020 0.0 21.126627 4 CAACGCA 22235 0.0 20.87973 5 CATGGGA 10080 0.0 20.772928 4 AACGCAG 22545 0.0 20.613644 6 GTACTTT 11975 0.0 20.512056 14-15 AGTACTT 11885 0.0 19.670887 12-13 ATGGGAT 4355 0.0 19.036446 5 CGTACAC 200 1.31553825E-5 18.94942 3 AGAGTAC 20395 0.0 18.396595 10-11 CATGGGG 4905 0.0 18.25403 4 ACTTTTT 13770 0.0 18.23383 16-17 CGCAGAG 25440 0.0 18.156137 8 >>END_MODULE